FastQCFastQC Report
Thu 26 May 2016
SRR937478_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937478_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1960358
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64610.32958265786147223No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50980.26005454105831693No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31330.1598177475746777No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN28980.14783014122930607No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33050.054.394621
GTATCAA60000.043.1204451
ATCAACG74800.034.3855253
TATCAAC76200.033.691382
TCAACGC76650.033.494444
CAACGCA77650.033.0622375
AACGCAG80400.031.9643386
CGCAGAG93350.027.224758
ACGCAGA93850.027.0797087
GTACATG65750.025.0274121
TACATGG66200.024.2020362
TGGTATC16650.023.9852942
GCAGAGT102900.023.8209349
ACATGGG67250.022.4809573
GAGTACT63050.022.11185812-13
GTGGTAT20450.022.093571
GTACTTT65050.021.35735714-15
TATACCG1353.774515E-421.1299025
AGAGTAC93400.020.75067310-11
CAGAGTA97750.020.58048210-11
Produced by FastQC (version 0.11.5)