##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937478_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1960358 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.55392688478329 31.0 31.0 34.0 30.0 34.0 2 31.664450064733074 33.0 31.0 34.0 30.0 34.0 3 31.70937604253917 33.0 31.0 34.0 30.0 34.0 4 35.35090733427262 37.0 35.0 37.0 33.0 37.0 5 35.34952646404381 37.0 35.0 37.0 33.0 37.0 6 35.38050805006024 37.0 35.0 37.0 33.0 37.0 7 35.318658632759934 37.0 35.0 37.0 33.0 37.0 8 35.308745137367765 37.0 35.0 37.0 33.0 37.0 9 36.90744904757192 39.0 37.0 39.0 33.0 39.0 10-11 36.77703256241972 39.0 37.0 39.0 33.0 39.0 12-13 36.63274080550593 39.0 37.0 39.0 32.0 39.0 14-15 37.7761291560011 40.0 37.5 41.0 33.0 41.0 16-17 37.720299302474345 40.0 37.0 41.0 32.0 41.0 18-19 37.671358751819824 40.0 37.0 41.0 32.0 41.0 20-21 37.59245811224277 40.0 37.0 41.0 32.0 41.0 22-23 37.438356157395745 40.0 37.0 41.0 32.0 41.0 24-25 37.27035699601807 39.5 37.0 41.0 31.5 41.0 26-27 37.10553072449012 39.0 36.5 41.0 31.0 41.0 28-29 37.169875604353905 39.0 37.0 41.0 31.0 41.0 30-31 37.02697849066344 39.0 36.5 41.0 31.0 41.0 32-33 36.82777584502422 39.0 36.0 41.0 30.5 41.0 34-35 36.71246042814629 39.0 36.0 41.0 30.0 41.0 36-37 36.71984020265686 39.0 36.0 41.0 30.0 41.0 38-39 36.63839563998005 39.0 36.0 41.0 30.0 41.0 40-41 36.455566789331336 39.0 35.5 41.0 30.0 41.0 42-43 36.22142205658355 39.0 35.0 41.0 30.0 41.0 44-45 36.009571721083596 39.0 35.0 41.0 29.0 41.0 46-47 35.6993829698453 39.0 35.0 40.0 27.5 41.0 48-49 35.40416189287875 39.0 35.0 40.0 27.0 41.0 50-51 34.55442475302981 38.0 34.0 39.5 25.0 40.5 52-53 34.662727420195694 38.0 34.0 39.5 26.0 40.5 54-55 34.98039567262714 38.0 34.0 40.0 26.0 41.0 56-57 34.76628656602519 38.0 34.0 40.0 25.5 41.0 58-59 34.52039754983528 38.0 34.0 40.0 25.0 41.0 60-61 34.219917739514926 38.0 34.0 40.0 23.5 41.0 62-63 33.8736116056353 37.5 33.0 40.0 23.0 41.0 64-65 33.49668478920687 37.0 33.0 40.0 22.0 41.0 66-67 33.116314469091876 36.0 32.5 39.0 21.5 41.0 68-69 32.61427938162315 36.0 32.0 39.0 19.5 40.0 70-71 32.16113026294177 35.0 31.0 38.5 18.0 40.0 72-73 31.75903559451896 35.0 31.0 38.0 17.5 40.0 74-75 31.279021484851235 35.0 31.0 37.0 12.5 39.0 76-77 30.822594138417575 35.0 30.5 36.5 8.0 39.0 78-79 30.32624806285383 34.0 30.0 36.0 7.0 38.5 80-81 29.826810970241148 34.0 29.5 35.5 3.5 37.5 82-83 29.32212075549466 34.0 29.0 35.0 2.0 37.0 84-85 28.859388183178787 34.0 29.0 35.0 2.0 36.5 86-87 28.387994182695202 34.0 28.0 35.0 2.0 36.0 88-89 27.94847267692942 33.5 27.0 35.0 2.0 36.0 90-91 27.485090223316355 33.0 26.0 35.0 2.0 35.0 92-93 26.978502140935483 33.0 25.5 35.0 2.0 35.0 94-95 26.386725536866226 32.5 25.0 35.0 2.0 35.0 96-97 25.675619708236965 32.0 23.5 34.0 2.0 35.0 98-99 24.841596279863168 32.0 18.0 34.0 2.0 35.0 100-101 22.85703708200237 30.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1346827946097342 1101 2 0.1370454787398856 1101 3 0.1502155814719046 1101 4 0.05406020934123745 1101 5 0.1120754506490016 1101 6 0.17446736894694936 1101 7 0.05671815694466176 1101 8 0.08450098780545545 1101 9 0.12510735543100537 1101 10-11 0.2242677739497907 1101 12-13 0.1431522823747713 1101 14-15 0.26028677538220535 1101 16-17 0.4033887709881938 1101 18-19 0.3821910335748413 1101 20-21 0.3897851184152259 1101 22-23 0.41714800504306737 1101 24-25 0.3412721329853383 1101 26-27 0.3432168329270766 1101 28-29 0.3433511097395794 1101 30-31 0.2912752934083471 1101 32-33 0.30181384612218665 1101 34-35 0.17589127315671504 1101 36-37 0.29406440020319025 1101 38-39 0.47716417912882747 1101 40-41 0.28196725351482144 1101 42-43 0.38663795158632297 1101 44-45 0.6125429560023647 1101 46-47 0.7006802691878065 1101 48-49 0.6297735842092109 1101 50-51 0.4512846632639196 1101 52-53 0.5062526754550802 1101 54-55 0.5584202276947678 1101 56-57 0.4217875903069981 1101 58-59 0.4903500167572261 1101 60-61 0.5182077714325928 1101 62-63 0.5683346294244842 1101 64-65 0.5755533697140862 1101 66-67 0.5260325128686887 1101 68-69 0.6691731219912 1101 70-71 0.6781732797388642 1101 72-73 0.6839791356103966 1101 74-75 0.5272175711027884 1101 76-77 0.44005525346015517 1101 78-79 0.5408207475535463 1101 80-81 0.5715486858549141 1101 82-83 0.6021743872399554 1101 84-85 0.7180128249602973 1101 86-87 0.7215324908090892 1101 88-89 0.759687500392296 1101 90-91 0.8566975978511628 1101 92-93 1.0833035885943865 1101 94-95 1.0283304723198938 1101 96-97 1.1767548635781928 1101 98-99 1.3652217755855567 1101 100-101 1.7628198793950425 1102 1 0.01555209040074601 1102 2 -0.05884816856498887 1102 3 0.0058425724381194755 1102 4 -0.03736205462336528 1102 5 -0.10810096469196395 1102 6 -0.11832497025075384 1102 7 -0.006992762022406396 1102 8 0.06665127973314355 1102 9 0.12814669547108792 1102 10-11 0.18030675573699284 1102 12-13 0.11210095538671538 1102 14-15 0.15997838742337933 1102 16-17 0.23363451310419947 1102 18-19 0.2335461009167048 1102 20-21 0.1690059667967958 1102 22-23 0.2123353132083281 1102 24-25 0.08273312699186164 1102 26-27 0.18829085571970694 1102 28-29 0.2763103754179852 1102 30-31 0.30375943829743335 1102 32-33 0.15378568938169934 1102 34-35 0.13336471887171086 1102 36-37 0.1954407690681066 1102 38-39 0.1856508995656938 1102 40-41 0.1536522039887842 1102 42-43 0.34381656485716405 1102 44-45 0.1996763519492717 1102 46-47 0.18272453762572383 1102 48-49 0.07827424746117373 1102 50-51 0.031969991355779825 1102 52-53 0.09723922644802485 1102 54-55 -0.01284208119668051 1102 56-57 -0.055208217165962026 1102 58-59 0.019006858903260593 1102 60-61 0.032338427229028355 1102 62-63 -0.10442556383843282 1102 64-65 0.027097730135857034 1102 66-67 0.020536235793976232 1102 68-69 -0.0316808754332456 1102 70-71 0.06778249361583022 1102 72-73 0.07353751630734706 1102 74-75 0.2063909751829982 1102 76-77 0.1975441911919269 1102 78-79 0.1662799406131228 1102 80-81 0.03600564225457248 1102 82-83 0.10559231343986397 1102 84-85 0.20572765566160456 1102 86-87 0.10493424721649447 1102 88-89 0.06915651151750524 1102 90-91 0.25189458852690194 1102 92-93 0.34605739140037883 1102 94-95 0.25566468353417804 1102 96-97 0.36136644967023557 1102 98-99 0.3136135105946103 1102 100-101 0.795151234268733 1103 1 0.06337194310230387 1103 2 -0.07402586709665826 1103 3 0.13181439765390124 1103 4 0.020406495017695647 1103 5 6.376375562666681E-5 1103 6 -0.09509706734482393 1103 7 -0.16765979491781735 1103 8 -0.039883110077006734 1103 9 -0.03893275444092126 1103 10-11 0.15734281685374185 1103 12-13 0.1179049050706098 1103 14-15 0.0703865772912593 1103 16-17 -0.02215314250421585 1103 18-19 0.13183196417681842 1103 20-21 -0.03948790197537022 1103 22-23 -0.2156499941910468 1103 24-25 -0.07285993080089526 1103 26-27 0.10258029436553073 1103 28-29 0.005372488433692979 1103 30-31 0.027284824467024293 1103 32-33 0.013557944761075191 1103 34-35 -0.03705082660737702 1103 36-37 0.11205001851643459 1103 38-39 0.13030597726515936 1103 40-41 0.06986574017560088 1103 42-43 0.04225186806663572 1103 44-45 0.11206403921475072 1103 46-47 0.08854897129021566 1103 48-49 0.06131164934756583 1103 50-51 0.012926407031613962 1103 52-53 0.05041750225550601 1103 54-55 0.035061569607321985 1103 56-57 -0.06369929266804775 1103 58-59 -0.04170998730860731 1103 60-61 -0.04146535485866565 1103 62-63 -0.15068304747452288 1103 64-65 -0.06071476695448297 1103 66-67 0.027186546312499615 1103 68-69 0.01862778091074091 1103 70-71 0.19597357191118903 1103 72-73 0.1453618009974349 1103 74-75 0.26179129734014595 1103 76-77 0.34987517507476085 1103 78-79 0.14282671392063406 1103 80-81 0.05454690350912017 1103 82-83 0.059493115108374184 1103 84-85 0.10973413604819271 1103 86-87 0.16623674905539332 1103 88-89 0.16110770377828487 1103 90-91 0.2068358833006947 1103 92-93 0.14186050310324916 1103 94-95 0.2828669442207641 1103 96-97 0.5040280636216892 1103 98-99 0.5444302627663795 1103 100-101 0.8967643477406746 1104 1 0.0445945346323775 1104 2 0.021733752610536783 1104 3 0.022554780654825635 1104 4 -0.1042341820854773 1104 5 -0.07062476770103387 1104 6 -0.02857976875250756 1104 7 0.08278460820266531 1104 8 0.1169224953782404 1104 9 0.09920593399665023 1104 10-11 0.1402195304190954 1104 12-13 0.11928622876542505 1104 14-15 0.20602810474531452 1104 16-17 0.13880203082474196 1104 18-19 0.16404094467652186 1104 20-21 0.18775275454606088 1104 22-23 0.08152137046865704 1104 24-25 -0.0371639700578541 1104 26-27 -0.08468944740583595 1104 28-29 -0.020777245538276645 1104 30-31 0.008947847202627202 1104 32-33 -0.03906360937702402 1104 34-35 -0.02980948402790773 1104 36-37 -0.012670187156537338 1104 38-39 0.11375545902053119 1104 40-41 0.08152529651270157 1104 42-43 0.14348415468001718 1104 44-45 0.1660726041077183 1104 46-47 0.20255286015603957 1104 48-49 0.2719124195379834 1104 50-51 0.10641138326331401 1104 52-53 0.18314192899082826 1104 54-55 0.2275093344121828 1104 56-57 0.30481308074972446 1104 58-59 0.34889345489732193 1104 60-61 0.3732511316575895 1104 62-63 0.1552533405021279 1104 64-65 0.3081941660627763 1104 66-67 0.39118005403697254 1104 68-69 0.40440651997217003 1104 70-71 0.5251243584406282 1104 72-73 0.5298481330964684 1104 74-75 0.44947303501682967 1104 76-77 0.4952931198254227 1104 78-79 0.5422029427318193 1104 80-81 0.5569894506620727 1104 82-83 0.5110469462911595 1104 84-85 0.5630746970631222 1104 86-87 0.6139352048831874 1104 88-89 0.46766732131898436 1104 90-91 0.3723455792867121 1104 92-93 0.5742684404336522 1104 94-95 0.7337097123566174 1104 96-97 0.695266741407309 1104 98-99 0.6874962019708057 1104 100-101 0.941320567488674 1105 1 0.037855671481953124 1105 2 -0.05657879141225308 1105 3 0.00836285219452293 1105 4 -0.009286791221754243 1105 5 -0.040798555104537115 1105 6 -0.04326911674835543 1105 7 0.05195532881171516 1105 8 -0.0407219360449389 1105 9 0.03878385894336134 1105 10-11 0.02061490472148364 1105 12-13 -0.0018600218789330825 1105 14-15 0.08210347713028199 1105 16-17 0.04932911666886497 1105 18-19 0.05541871987866642 1105 20-21 0.006781475381913538 1105 22-23 0.05298771532402924 1105 24-25 0.1381146188661262 1105 26-27 0.2820011333176069 1105 28-29 0.16524612405785177 1105 30-31 -0.027869694446650328 1105 32-33 -0.008135597305887643 1105 34-35 -0.030122495242288494 1105 36-37 -0.1526673146079176 1105 38-39 0.11497182059963507 1105 40-41 -0.1073410197960385 1105 42-43 0.10877773952412184 1105 44-45 -0.009546809312844573 1105 46-47 -0.018322519526563497 1105 48-49 0.1301169979440786 1105 50-51 -6.958672569084001E-4 1105 52-53 0.015610278191147131 1105 54-55 0.04291106015926971 1105 56-57 -0.06960458424384797 1105 58-59 0.03677897292372734 1105 60-61 0.13436723306177356 1105 62-63 0.1070812723350798 1105 64-65 0.04881787041993135 1105 66-67 0.05405248819461406 1105 68-69 0.0288443965470222 1105 70-71 0.3067986786786392 1105 72-73 0.30919409705832024 1105 74-75 0.2992846201521253 1105 76-77 0.26083215912067104 1105 78-79 0.14334080109836123 1105 80-81 0.3553850088361905 1105 82-83 0.4342177176974573 1105 84-85 0.4632068593410139 1105 86-87 0.5557395338819404 1105 88-89 0.35283871016225987 1105 90-91 0.513057356056418 1105 92-93 0.760430697577192 1105 94-95 0.8954217692266404 1105 96-97 0.836802900283935 1105 98-99 0.7609187059388951 1105 100-101 1.0831089239723184 1106 1 -0.10824646615688138 1106 2 -0.12836105427156497 1106 3 -0.18546028822294502 1106 4 -0.20774944293521003 1106 5 -0.13418554174967312 1106 6 -0.146224681790315 1106 7 -0.1533976479874184 1106 8 -0.15007144479283596 1106 9 -0.16091423087078027 1106 10-11 -0.5734736342736824 1106 12-13 -0.5789119246190708 1106 14-15 -0.5655912174352196 1106 16-17 -0.5584066010599997 1106 18-19 -0.32068807271443944 1106 20-21 -0.18413783396406558 1106 22-23 -0.21808643192033372 1106 24-25 -0.18934872888433318 1106 26-27 -0.2743955129091731 1106 28-29 -0.15426795166347773 1106 30-31 -0.24414912848362746 1106 32-33 -0.16327794293954412 1106 34-35 -0.23760605123025158 1106 36-37 -0.15532598843129364 1106 38-39 -0.2185564803089619 1106 40-41 -0.2728715246379636 1106 42-43 -0.3179514050122094 1106 44-45 -0.1681977439989808 1106 46-47 0.007467526592783713 1106 48-49 -0.07401405335859579 1106 50-51 -0.1428520486942304 1106 52-53 -0.3517549625372709 1106 54-55 -0.2839855855159854 1106 56-57 -0.161176193876301 1106 58-59 -0.29722502680711926 1106 60-61 -0.36125330433173986 1106 62-63 -0.3783419369139338 1106 64-65 -0.04734140623106242 1106 66-67 0.173058269383084 1106 68-69 -0.20093247450743235 1106 70-71 -0.1139339030229749 1106 72-73 0.09968613234331869 1106 74-75 -0.08610202233525399 1106 76-77 0.02088250322327312 1106 78-79 0.0726927696917592 1106 80-81 0.28663729081170075 1106 82-83 0.031648006448023125 1106 84-85 -0.022149062654307272 1106 86-87 0.05879558857655098 1106 88-89 0.30732412261658126 1106 90-91 0.2534904322641971 1106 92-93 0.4161441575649931 1106 94-95 0.43254034150142573 1106 96-97 0.602574867090361 1106 98-99 0.8066321218039043 1106 100-101 0.7160608157920656 1107 1 -0.039317616617889684 1107 2 -0.052009383100600104 1107 3 -0.0025039853683317403 1107 4 0.025230744696621343 1107 5 0.04049943003575862 1107 6 0.09450878808978302 1107 7 0.11301577460299939 1107 8 0.09615225195992849 1107 9 0.19021598806209994 1107 10-11 0.03350835507740868 1107 12-13 0.06246905175572692 1107 14-15 0.16784539847356683 1107 16-17 0.26081235581870743 1107 18-19 0.07034990080944681 1107 20-21 -0.14608087695012273 1107 22-23 0.10961216618502334 1107 24-25 0.012694128476653077 1107 26-27 0.044476078602237124 1107 28-29 0.07096495218012677 1107 30-31 0.17689205148980136 1107 32-33 0.1366766868900342 1107 34-35 0.13383222679728135 1107 36-37 0.07898040418209717 1107 38-39 0.17174477326012294 1107 40-41 -0.032216580052832455 1107 42-43 0.1146368105222706 1107 44-45 0.23857746207539066 1107 46-47 0.33617986444350123 1107 48-49 0.40243435661324867 1107 50-51 0.2806610421268161 1107 52-53 0.31016637692975735 1107 54-55 0.5067314158647491 1107 56-57 0.36848128314960604 1107 58-59 0.33495867439540206 1107 60-61 0.4050788663909728 1107 62-63 0.48706243563623985 1107 64-65 0.41461036178776567 1107 66-67 0.6161683291277456 1107 68-69 0.5945838402132182 1107 70-71 0.4992379517663039 1107 72-73 0.3680921342933594 1107 74-75 0.5376625675554898 1107 76-77 0.6851835953792502 1107 78-79 0.7072894877706801 1107 80-81 0.8387917195292722 1107 82-83 0.7365395870095242 1107 84-85 0.7585308791497241 1107 86-87 0.9362432329267634 1107 88-89 0.7650709701531753 1107 90-91 0.9771135675610942 1107 92-93 1.0335143890619278 1107 94-95 1.09191506202772 1107 96-97 0.9586347295402149 1107 98-99 0.9117496807142338 1107 100-101 1.1402860874132408 1108 1 -0.01913385208595031 1108 2 0.04810990227605316 1108 3 -0.01372048761987088 1108 4 0.02515561682898948 1108 5 -0.2607560715337982 1108 6 -0.12987216711951532 1108 7 -0.030374334185687246 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48-49 -0.311621390455997 2316 50-51 -0.15971335958142419 2316 52-53 -0.1665040144518244 2316 54-55 -0.3236783178558298 2316 56-57 -0.37131458136125417 2316 58-59 -0.33236584930855884 2316 60-61 -0.5347835755140267 2316 62-63 -0.6558116720155596 2316 64-65 -0.5437210865683824 2316 66-67 -0.6754067276477684 2316 68-69 -0.6444644423463686 2316 70-71 -0.9443318632646545 2316 72-73 -0.6212743324149237 2316 74-75 -0.8675023453284076 2316 76-77 -0.801684522569051 2316 78-79 -0.8680338235159581 2316 80-81 -1.1832753313711422 2316 82-83 -0.9214622165366464 2316 84-85 -0.9343957816659696 2316 86-87 -1.2307510427745214 2316 88-89 -1.3082621822223466 2316 90-91 -1.3727317889107091 2316 92-93 -1.658703143337064 2316 94-95 -1.6633368614029997 2316 96-97 -1.9092773792516198 2316 98-99 -1.8513120801862115 2316 100-101 -2.5050245417469057 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8145.0 3 1802.0 4 1688.0 5 1777.0 6 2131.0 7 2433.0 8 2992.0 9 3653.0 10 4482.0 11 5648.0 12 7185.0 13 8972.0 14 9987.0 15 12293.0 16 14246.0 17 17636.0 18 15888.0 19 12266.0 20 12894.0 21 13397.0 22 14979.0 23 16309.0 24 18495.0 25 21176.0 26 24759.0 27 29245.0 28 34959.0 29 41937.0 30 51641.0 31 63938.0 32 80866.0 33 104562.0 34 140055.0 35 193076.0 36 275269.0 37 371432.0 38 284781.0 39 33353.0 40 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.85466378772848 19.18671019883307 14.672397924134039 23.286228089304416 2 15.134291838312535 20.12031541735082 40.57009879945537 24.175293944881275 3 19.21089362607856 24.265578695744715 29.47334921492542 27.050178463251306 4 12.671006655297349 16.48643075589585 37.45903438578825 33.38352820301855 5 13.830873722225121 37.092183993442404 34.36205148042289 14.714890803909588 6 31.925764712318067 36.100771115702116 17.692307299039825 14.28115687293999 7 28.489967468137788 32.306173692744636 20.904635228060418 18.29922361105715 8 26.190608863153148 35.12799054864193 19.9352187313566 18.746181856848324 9 26.000306709265175 16.887003194888177 20.39861980830671 36.71407028753993 10-11 24.632476346956132 26.885539182539965 27.515605295254975 20.966379175248928 12-13 25.411473903368908 23.996170849634275 28.18471944060663 22.407635806390182 14-15 22.516608427580458 25.569195370216296 26.26527833477877 25.648917867424476 16-17 22.051206156578363 28.74736440403517 26.908626453523667 22.292802985862803 18-19 21.968572363309207 27.390862061914696 29.136225616488776 21.50433995828733 20-21 22.575809852405598 26.56359338061466 29.402491853555684 21.458104913424062 22-23 22.25227569201075 26.382309101541257 29.221914730045267 22.143500476402732 24-25 22.04423439023742 26.616917075723777 29.314087375008434 22.024761159030366 26-27 21.92089939233977 26.845277919327216 29.156881014821717 22.0769416735113 28-29 21.889085362954738 27.012789899358587 29.198482940751568 21.899641796935107 30-31 22.11942585483583 26.766889077320016 29.160447289033044 21.95323777881111 32-33 21.834787810002915 26.788980741604167 29.126393766966764 22.249837681426154 34-35 22.132941009552027 26.91912923549269 29.07175460505377 21.876175149901513 36-37 22.130063460737837 26.719242652281107 29.047286440726914 22.10340744625414 38-39 21.919665070510867 26.960040051695106 29.04529915207045 22.07499572572358 40-41 22.242357024024614 26.875394511214978 28.96171037062971 21.920538094130695 42-43 21.941478350209827 26.87510542484295 28.83357953761303 22.349836687334196 44-45 22.074330910877233 27.05907490700457 28.78546464998091 22.081129532137286 46-47 22.079673443931963 27.20286682283899 28.395169648893436 22.32229008433561 48-49 22.143279290628143 27.213975165441024 28.391847786075957 22.250897757854872 50-51 22.314395019089993 27.360314540734482 28.196056225690135 22.12923421448539 52-53 22.316262042024643 27.400318742743206 28.045193660634858 22.23822555459729 54-55 22.207450139748275 27.27775372095437 28.1989624834549 22.31583365584246 56-57 22.145513348638765 27.561606743112044 28.083148148526732 22.20973175972246 58-59 22.238253568017925 27.28784639142669 28.254071446478008 22.21982859407738 60-61 22.24185611026187 27.354341678715315 28.308267544901977 22.095534666120837 62-63 22.08861215621312 27.387896943543023 28.46950724285081 22.053983657393044 64-65 22.040113122408986 27.237820520519474 28.377477427520574 22.34458892955097 66-67 22.042226564037197 27.38782930091384 28.16623847603316 22.40370565901579 68-69 21.96741234075021 27.365104333556662 28.292252522418515 22.375230803274608 70-71 22.08436046039144 27.178126959804427 28.328390004850473 22.409122574953656 72-73 22.01364598983259 27.301920655563595 28.212786260273525 22.471647094330287 74-75 22.00345981940346 27.52107696526457 27.937747784839 22.537715430492973 76-77 22.12506919522945 27.33067167036134 28.024464059627245 22.51979507478196 78-79 22.078714657450014 27.28666943697772 28.0831083094509 22.551507596121358 80-81 22.03035200598637 27.587321691804114 27.886614694823876 22.495711607385644 82-83 22.1117074682811 27.416696757106646 27.955922552446168 22.51567322216609 84-85 22.195630326311182 27.364415188248394 27.89795662318355 22.541997862256874 86-87 22.04749117337342 27.37801470469865 28.02769837199622 22.546795749931707 88-89 22.13993437358098 27.232153010083653 27.9766022216697 22.651310394665664 90-91 22.190579384225376 27.26609457415055 27.938831418407815 22.604494623216258 92-93 22.12955379718937 27.171595727962323 28.01864384907579 22.680206625772513 94-95 22.083343341675608 27.18026412824443 27.937968039061666 22.798424491018302 96-97 22.00230181354697 27.150003219677032 27.997417103537657 22.85027786323834 98-99 22.00456268944655 27.09494886716042 27.932251191719594 22.968237251673443 100-101 22.20364309820748 26.903749803249806 28.141512963027317 22.751094135515405 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2824.0 1 1578.5 2 370.5 3 401.5 4 346.0 5 337.0 6 524.0 7 1002.5 8 1673.5 9 2096.5 10 2014.0 11 1830.0 12 1924.0 13 2084.5 14 2190.5 15 2342.0 16 2493.5 17 2578.5 18 2703.0 19 2952.5 20 3321.5 21 3839.0 22 4442.5 23 5413.5 24 6703.0 25 8357.5 26 10712.0 27 13830.0 28 16437.0 29 19293.5 30 23177.0 31 26621.0 32 30151.0 33 34089.0 34 38498.5 35 42819.0 36 48138.5 37 53226.0 38 56018.0 39 58589.5 40 60706.0 41 63622.0 42 66841.0 43 69352.5 44 72011.5 45 74212.0 46 76096.5 47 77791.0 48 78204.0 49 77274.5 50 75116.0 51 71305.0 52 67977.5 53 66090.0 54 63981.0 55 58884.5 56 51840.0 57 45704.5 58 40735.0 59 35688.0 60 29793.0 61 24276.5 62 19217.5 63 14462.5 64 10884.5 65 8163.5 66 6082.0 67 4665.0 68 3486.0 69 2477.5 70 1842.5 71 1391.0 72 1021.0 73 794.0 74 594.5 75 397.0 76 248.5 77 161.0 78 104.5 79 60.5 80 43.0 81 37.5 82 29.5 83 25.5 84 19.0 85 13.5 86 13.5 87 11.5 88 10.0 89 8.5 90 6.0 91 5.0 92 4.5 93 5.0 94 3.5 95 1.5 96 6.5 97 10.5 98 7.5 99 4.5 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3499360831031883 2 0.3058114895340545 3 0.30065936936008625 4 0.30479126771742715 5 0.3052503675349095 6 0.22245936711559827 7 0.21026771640690117 8 0.2161339918525086 9 0.20955356113526202 10-11 0.2311567581023466 12-13 0.21988330702861417 14-15 0.21411905376466953 16-17 0.21457815358215185 18-19 0.2100636720435757 20-21 0.20317717478134095 22-23 0.2055746960504153 24-25 0.1951684335208161 26-27 0.19572955551996116 28-29 0.21450163694590477 30-31 0.22656575992752342 32-33 0.22077600111816312 34-35 0.22906530337826048 36-37 0.20157032542015285 38-39 0.1982546045161139 40-41 0.195015400248322 42-43 0.2034322302354978 44-45 0.20807423950115234 46-47 0.2135069206746931 48-49 0.22312251129640606 50-51 0.22906530337826048 52-53 0.2480414291675296 54-55 0.2214391452989709 56-57 0.19149563498095756 58-59 0.19254136234300062 60-61 0.18909811371188326 62-63 0.1977699991532159 64-65 0.18958271907478125 66-67 0.18746575880527944 68-69 0.18389498244708366 70-71 0.19634168860993756 72-73 0.19447978379459263 74-75 0.19881572651525894 76-77 0.200830664603098 78-79 0.198892243151506 80-81 0.1996574095139765 82-83 0.19162316270803598 84-85 0.18473666544580122 86-87 0.18550183180827173 88-89 0.1875422754415265 90-91 0.1937401229775378 92-93 0.18774631980485196 94-95 0.18654755917031482 96-97 0.18568037062618156 98-99 0.1957040499745455 100-101 0.1817015055413348 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1960358.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.25288983981555 #Duplication Level Percentage of deduplicated Percentage of total 1 80.69484860611857 50.23487520917293 2 11.319040275239137 14.092859346937953 3 3.4524857941948324 6.44781653458617 4 1.4090880554880352 3.508792139715862 5 0.8003124494529527 2.491088137661381 6 0.5061693239601314 1.8906301898870377 7 0.31147617980041076 1.3573204616189085 8 0.21918035444301118 1.0915688368154 9 0.19475031265007733 1.0911392783707412 >10 1.0033962142194748 11.311177988129186 >50 0.06254878591033215 2.645487828197434 >100 0.0258849822485858 2.6960505164671895 >500 4.0933313716599265E-4 0.17547022741114504 >1k 2.4559988229959275E-4 0.3766264828836336 >5k 1.6373325486639519E-4 0.5890968221450502 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6461 0.32958265786147223 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5098 0.26005454105831693 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3133 0.1598177475746777 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2898 0.14783014122930607 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.5505545415684277E-5 0.0 12-13 0.0 0.0 0.0 1.020221816627371E-4 0.0 14-15 0.0 0.0 0.0 1.275277270784214E-4 0.0 16-17 0.0 0.0 0.0 2.040443633254742E-4 0.0 18-19 0.0 0.0 0.0 3.060665449882113E-4 0.0 20-21 0.0 0.0 0.0 3.315720904038956E-4 0.0 22-23 0.0 0.0 0.0 4.59099817482317E-4 0.0 24-25 0.0 0.0 0.0 7.396608170548441E-4 0.0 26-27 0.0 0.0 0.0 8.416829987175811E-4 0.0 28-29 0.0 0.0 0.0 0.0016833659974351623 0.0 30-31 0.0 5.101109083136855E-5 0.0 0.003927853994015379 0.0 32-33 0.0 5.101109083136855E-5 0.0 0.007677169170120968 0.0 34-35 0.0 5.101109083136855E-5 0.0 0.011885584163708874 0.0 36-37 0.0 5.101109083136855E-5 0.0 0.017573320791406468 0.0 38-39 0.0 5.101109083136855E-5 0.0 0.02545453432485291 0.0 40-41 0.0 5.101109083136855E-5 0.0 0.037008546398157885 0.0 42-43 0.0 5.101109083136855E-5 0.0 0.04960828583350592 0.0 44-45 0.0 5.101109083136855E-5 0.0 0.06501363526457923 0.0 46-47 0.0 5.101109083136855E-5 0.0 0.08577514923294623 0.0 48-49 5.101109083136855E-5 5.101109083136855E-5 0.0 0.10689374083713281 0.0 50-51 5.101109083136855E-5 5.101109083136855E-5 0.0 0.13308793597904056 0.0 52-53 5.101109083136855E-5 7.651663624705283E-5 0.0 0.1712697374663199 0.0 54-55 5.101109083136855E-5 1.020221816627371E-4 0.0 0.222076783934363 0.0 56-57 5.101109083136855E-5 1.020221816627371E-4 0.0 0.2723992250395081 0.0 58-59 5.101109083136855E-5 1.020221816627371E-4 0.0 0.3413407142981027 0.0 60-61 5.101109083136855E-5 1.020221816627371E-4 0.0 0.4196937498150848 2.5505545415684277E-5 62-63 5.101109083136855E-5 1.020221816627371E-4 0.0 0.49016557179862047 5.101109083136855E-5 64-65 5.101109083136855E-5 1.020221816627371E-4 0.0 0.5670902967723243 5.101109083136855E-5 66-67 5.101109083136855E-5 1.020221816627371E-4 0.0 0.6812021069620957 5.101109083136855E-5 68-69 5.101109083136855E-5 1.020221816627371E-4 0.0 0.8050570355006585 5.101109083136855E-5 70-71 5.101109083136855E-5 1.020221816627371E-4 0.0 0.9166948077851087 5.101109083136855E-5 72-73 5.101109083136855E-5 1.020221816627371E-4 0.0 1.0556745247551724 5.101109083136855E-5 74-75 5.101109083136855E-5 1.020221816627371E-4 0.0 1.2177112547810145 5.101109083136855E-5 76-77 5.101109083136855E-5 1.020221816627371E-4 0.0 1.3897206530643893 5.101109083136855E-5 78-79 5.101109083136855E-5 1.020221816627371E-4 0.0 1.5906023287583186 5.101109083136855E-5 80-81 5.101109083136855E-5 1.020221816627371E-4 0.0 1.8176781995941558 7.651663624705283E-5 82-83 5.101109083136855E-5 1.020221816627371E-4 0.0 2.073422303477222 1.020221816627371E-4 84-85 5.101109083136855E-5 1.020221816627371E-4 0.0 2.3356958269867034 1.020221816627371E-4 86-87 5.101109083136855E-5 1.020221816627371E-4 0.0 2.6118188616569014 1.020221816627371E-4 88-89 5.101109083136855E-5 1.020221816627371E-4 0.0 2.9284957135380374 1.020221816627371E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3305 0.0 54.39462 1 GTATCAA 6000 0.0 43.120445 1 ATCAACG 7480 0.0 34.385525 3 TATCAAC 7620 0.0 33.69138 2 TCAACGC 7665 0.0 33.49444 4 CAACGCA 7765 0.0 33.062237 5 AACGCAG 8040 0.0 31.964338 6 CGCAGAG 9335 0.0 27.22475 8 ACGCAGA 9385 0.0 27.079708 7 GTACATG 6575 0.0 25.027412 1 TACATGG 6620 0.0 24.202036 2 TGGTATC 1665 0.0 23.985294 2 GCAGAGT 10290 0.0 23.820934 9 ACATGGG 6725 0.0 22.480957 3 GAGTACT 6305 0.0 22.111858 12-13 GTGGTAT 2045 0.0 22.09357 1 GTACTTT 6505 0.0 21.357357 14-15 TATACCG 135 3.774515E-4 21.129902 5 AGAGTAC 9340 0.0 20.750673 10-11 CAGAGTA 9775 0.0 20.580482 10-11 >>END_MODULE