FastQCFastQC Report
Thu 26 May 2016
SRR937478_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937478_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1960358
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69030.35212956000893714No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61770.31509550806536357No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39430.2011367311480862No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34900.057.7433171
GTATCAA62850.042.0465931
ATCAACG80800.032.158333
TCAACGC81600.031.959484
TATCAAC82950.031.6111432
CAACGCA83450.031.194055
TGGTATC14400.030.3488922
AACGCAG87250.029.8316486
GTGGTAT18350.025.6425041
ACGCAGA104150.024.5868237
CGCAGAG104800.024.2529958
GCAGAGT113500.021.9753769
GAGTACT69750.021.21713412-13
GTACATG68900.021.0398161
TACATGG68850.020.6983072
GTACTTT72850.020.01570914-15
CAGAGTA110050.019.90140710-11
ACATGGG68600.019.8735433
AGTACTT72050.019.35294312-13
AGAGTAC105500.019.34120810-11
Produced by FastQC (version 0.11.5)