##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937478_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1960358 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.778008404587325 31.0 31.0 34.0 30.0 34.0 2 32.0265002616869 31.0 31.0 34.0 30.0 34.0 3 32.10594544465858 33.0 31.0 34.0 30.0 34.0 4 35.81495369723285 37.0 35.0 37.0 35.0 37.0 5 35.61966589775949 37.0 35.0 37.0 33.0 37.0 6 35.71165368774479 37.0 35.0 37.0 33.0 37.0 7 35.66630278755207 37.0 35.0 37.0 33.0 37.0 8 35.63259618906343 37.0 35.0 37.0 33.0 37.0 9 37.2694410918822 39.0 37.0 39.0 34.0 39.0 10-11 37.16769181955541 39.0 37.0 39.0 33.0 39.0 12-13 37.059792905173445 39.0 37.0 39.0 33.0 39.0 14-15 38.17467472777932 40.0 37.0 41.0 33.0 41.0 16-17 38.13179863065828 40.0 37.0 41.0 33.0 41.0 18-19 38.115977285781476 40.0 37.0 41.0 33.0 41.0 20-21 38.00828955731555 40.0 37.0 41.0 33.0 41.0 22-23 37.85968787333742 40.0 37.0 41.0 33.0 41.0 24-25 37.702374260211656 39.0 37.0 41.0 32.0 41.0 26-27 37.44312952022028 39.0 36.0 40.5 32.0 41.0 28-29 37.424241898673614 39.0 36.0 41.0 32.0 41.0 30-31 37.57979537411023 39.0 37.0 41.0 32.0 41.0 32-33 37.55522333165677 39.5 36.5 41.0 32.0 41.0 34-35 37.438387274161144 39.0 36.0 41.0 32.0 41.0 36-37 37.275349961588645 39.0 36.0 41.0 31.5 41.0 38-39 37.125832883585545 39.0 36.0 41.0 31.0 41.0 40-41 36.95608047101601 39.0 36.0 41.0 31.0 41.0 42-43 36.74463235796727 39.0 35.5 40.0 30.5 41.0 44-45 36.46940584321843 39.0 35.0 40.0 30.0 41.0 46-47 36.1696024399625 39.0 35.0 40.0 30.0 41.0 48-49 35.870858282007674 39.0 35.0 40.0 29.0 41.0 50-51 35.649900936461606 38.5 35.0 40.0 28.0 41.0 52-53 35.399661694445605 38.0 34.5 40.0 27.5 41.0 54-55 35.174668606448414 38.0 34.0 40.0 27.0 41.0 56-57 34.90975066799024 38.0 34.0 40.0 26.5 41.0 58-59 34.62840613806254 38.0 34.0 40.0 26.0 41.0 60-61 34.25621544636235 37.0 33.0 40.0 26.0 41.0 62-63 33.85066809225662 37.0 33.0 39.5 24.5 41.0 64-65 33.471921455162786 36.0 32.0 39.0 23.5 40.0 66-67 33.05122151158105 36.0 32.0 39.0 22.5 40.0 68-69 32.57500772818026 35.0 31.0 38.5 22.0 40.0 70-71 32.16753062450838 35.0 31.0 38.0 21.5 40.0 72-73 31.704931446195033 35.0 31.0 37.0 20.0 39.0 74-75 31.2275584357551 34.0 30.0 37.0 18.5 39.0 76-77 29.984800225264976 33.5 28.5 35.5 18.0 37.5 78-79 30.646749216214587 34.0 30.0 36.0 18.0 38.0 80-81 30.47064923855745 34.0 30.0 35.5 18.0 37.0 82-83 30.095566473062576 34.0 30.0 35.0 15.5 37.0 84-85 29.70248444416785 34.0 29.0 35.0 10.0 36.0 86-87 29.30163342613951 34.0 29.0 35.0 7.0 36.0 88-89 28.866540448224253 33.5 29.0 35.0 3.5 35.5 90-91 28.318070474882646 33.0 28.0 35.0 2.0 35.0 92-93 27.775681533679055 33.0 27.0 35.0 2.0 35.0 94-95 27.23947386140695 32.5 26.0 34.0 2.0 35.0 96-97 26.657722211963325 32.0 25.0 34.0 2.0 35.0 98-99 25.8061272481863 32.0 24.5 34.0 2.0 35.0 100-101 24.02568816512086 30.5 17.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.146284794492491 1101 2 0.19071985772210098 1101 3 0.15585252331722188 1101 4 0.08281088602753783 1101 5 0.02958798357695258 1101 6 0.07044509336012084 1101 7 0.10903058185427739 1101 8 0.10615913193795024 1101 9 0.15678925991593218 1101 10-11 0.13796451121920938 1101 12-13 0.21115953508268603 1101 14-15 0.295913138603936 1101 16-17 0.1931974223199262 1101 18-19 0.24441121803000243 1101 20-21 0.15164519780936558 1101 22-23 0.15997618471033803 1101 24-25 0.2001451531020706 1101 26-27 0.2123086270648642 1101 28-29 0.09212624747480902 1101 30-31 0.19191269823099333 1101 32-33 0.20913364949627322 1101 34-35 0.18233852607909995 1101 36-37 0.31822867507572283 1101 38-39 0.20009455387199893 1101 40-41 0.22657501399533686 1101 42-43 0.17323196829962484 1101 44-45 0.17900186545524832 1101 46-47 0.24093360706173428 1101 48-49 0.350908440512093 1101 50-51 0.1795912220822089 1101 52-53 0.26805740615002094 1101 54-55 0.23902520589440002 1101 56-57 0.30971236929046597 1101 58-59 0.2549164086110238 1101 60-61 0.09350917190991481 1101 62-63 0.12659739222228694 1101 64-65 0.2200658664146431 1101 66-67 0.11050481342516605 1101 68-69 0.0012953611459778358 1101 70-71 -0.062273185046223034 1101 72-73 -0.2410656834453384 1101 74-75 -0.1680419158097095 1101 76-77 -0.09807753677477749 1101 78-79 -0.051828291837207274 1101 80-81 -0.14646443101031892 1101 82-83 -0.11747001235115206 1101 84-85 -0.25291076961699943 1101 86-87 -0.25982097742605603 1101 88-89 -0.24156170425805712 1101 90-91 -0.21342434418523837 1101 92-93 -0.11277929788328223 1101 94-95 -0.2361303668674033 1101 96-97 -0.26105382269525634 1101 98-99 -0.3453978815525929 1101 100-101 0.19341959672328102 1102 1 0.14532138469959932 1102 2 0.12699828799350144 1102 3 0.028906096647716595 1102 4 0.0027781591421103258 1102 5 -0.018701600946528174 1102 6 0.03932259992001974 1102 7 0.1071182860342148 1102 8 0.09060224234761449 1102 9 0.11540233711458825 1102 10-11 0.08589439047830894 1102 12-13 0.0846764165397147 1102 14-15 0.06382094173913799 1102 16-17 0.08656538676574854 1102 18-19 0.049826162016678666 1102 20-21 0.10329267696776157 1102 22-23 0.11044627059420264 1102 24-25 0.14033676998449351 1102 26-27 0.06648808271412321 1102 28-29 0.06876477031100592 1102 30-31 0.002559102630726784 1102 32-33 -7.773682324057063E-4 1102 34-35 0.12372980601015371 1102 36-37 0.14647255814973192 1102 38-39 0.08280643140761157 1102 40-41 0.11217324790542449 1102 42-43 0.286788935350053 1102 44-45 0.24934917710621107 1102 46-47 0.19099267037567813 1102 48-49 0.27775562144916677 1102 50-51 0.198325911612514 1102 52-53 0.2778599797613097 1102 54-55 0.252866838752972 1102 56-57 0.27787675895341835 1102 58-59 0.19917564814664956 1102 60-61 0.22496135483190471 1102 62-63 0.31072001153501816 1102 64-65 0.2629492212108744 1102 66-67 0.28812320632206934 1102 68-69 0.21225354238320904 1102 70-71 0.30105430650578313 1102 72-73 0.4158984622183475 1102 74-75 0.3941522378904594 1102 76-77 0.19947714520316495 1102 78-79 0.19820753345156916 1102 80-81 0.22089437378058818 1102 82-83 0.20332027415433274 1102 84-85 0.3494817787229323 1102 86-87 0.26083278568164303 1102 88-89 0.36363635149384166 1102 90-91 0.22984146977500686 1102 92-93 0.3016946029097163 1102 94-95 0.2014527816913514 1102 96-97 0.18436102446631608 1102 98-99 0.1106280288454542 1102 100-101 0.2978541865237254 1103 1 0.10030783733970239 1103 2 0.04666849590590516 1103 3 0.06869250459549647 1103 4 0.09414509241089064 1103 5 0.003544933070848799 1103 6 0.016660799750560784 1103 7 0.02357064681298482 1103 8 -0.010639831748527229 1103 9 0.06937110835507809 1103 10-11 0.042604756303575186 1103 12-13 0.0491958344222283 1103 14-15 0.07569746057974669 1103 16-17 0.014709910559751904 1103 18-19 0.014694738659351003 1103 20-21 -0.04312122979288091 1103 22-23 0.09009357369193793 1103 24-25 0.08440945087200902 1103 26-27 -0.060930656403762384 1103 28-29 -0.14768246947260621 1103 30-31 0.017489596760206894 1103 32-33 0.16152279657053015 1103 34-35 0.19989250468149322 1103 36-37 0.10243293095817307 1103 38-39 0.25424524482647826 1103 40-41 0.00987438662852469 1103 42-43 0.012057322050360142 1103 44-45 0.06573449940302822 1103 46-47 -0.043261666176121594 1103 48-49 -0.06863826687590091 1103 50-51 -0.007204763148429549 1103 52-53 0.11113155078950854 1103 54-55 0.010974770708905623 1103 56-57 0.03976026096922425 1103 58-59 -0.16141829418162956 1103 60-61 -0.25800109430730345 1103 62-63 -0.13313246656108646 1103 64-65 -0.1374637172161428 1103 66-67 -0.1679642602750775 1103 68-69 -0.08108683619798995 1103 70-71 -0.08046634972646416 1103 72-73 -0.27091264754785627 1103 74-75 -0.32755193618613276 1103 76-77 -0.4354699223287959 1103 78-79 -0.03946297240964114 1103 80-81 -0.09300540368889898 1103 82-83 -0.20154094687712742 1103 84-85 -0.2746213488084699 1103 86-87 -0.0048500291897255465 1103 88-89 -0.1526330500945967 1103 90-91 0.005029317187972282 1103 92-93 -0.05212659056147828 1103 94-95 -0.10377109890566416 1103 96-97 -0.010307554038536892 1103 98-99 -0.11914823022103604 1103 100-101 0.0752529072060284 1104 1 -0.054586915941186476 1104 2 0.026383384629099282 1104 3 0.06922275524138399 1104 4 0.013119110670871237 1104 5 -0.12622908302262914 1104 6 -0.11260733881150031 1104 7 -0.12073317579407217 1104 8 -0.01760891761229999 1104 9 -0.10831340966206682 1104 10-11 0.014621803040377301 1104 12-13 -0.006660212547473066 1104 14-15 -0.054601570453549186 1104 16-17 -0.05647655459842582 1104 18-19 -0.1599274329309779 1104 20-21 -0.12503096260938662 1104 22-23 -0.13609633078350925 1104 24-25 -0.07829742722089605 1104 26-27 -0.2813941427701607 1104 28-29 -0.22310615544579093 1104 30-31 -0.20539359824818604 1104 32-33 -0.23354805697594117 1104 34-35 -0.26469056160074445 1104 36-37 -0.21083750770372234 1104 38-39 -0.3434715359725615 1104 40-41 -0.27884853438994384 1104 42-43 -0.09146666921865432 1104 44-45 -0.0762878191481633 1104 46-47 -0.12291697617506259 1104 48-49 -0.3070041669028001 1104 50-51 -0.25708398082561246 1104 52-53 -0.04436639193908576 1104 54-55 -0.23355111805219764 1104 56-57 -0.2557549054269259 1104 58-59 -0.31777209431125897 1104 60-61 -0.3637760781036974 1104 62-63 -0.3988100662245557 1104 64-65 -0.17482093138720955 1104 66-67 -0.3389286592792047 1104 68-69 -0.32802503139594563 1104 70-71 -0.34555448110296894 1104 72-73 -0.19641920211023134 1104 74-75 -0.4213510547820931 1104 76-77 -0.24585395234202778 1104 78-79 -0.37440264443779014 1104 80-81 -0.2696054986537746 1104 82-83 -0.32009800433809943 1104 84-85 -0.3011538309483903 1104 86-87 -0.2806412075666245 1104 88-89 -0.10882093250425484 1104 90-91 -0.03260394507215736 1104 92-93 -0.057686278513401135 1104 94-95 -0.2917309608996739 1104 96-97 -0.14623587180617648 1104 98-99 -0.09012960013449955 1104 100-101 0.1964147284373574 1105 1 -0.06920310874464874 1105 2 -0.03959347686647874 1105 3 -0.0756064604741411 1105 4 -0.05241506353578984 1105 5 -0.273861984901302 1105 6 -0.07246391886170045 1105 7 -0.013746479307279458 1105 8 -0.10381166946380915 1105 9 -0.11836214282519819 1105 10-11 -0.01386051826509771 1105 12-13 0.002934654354795896 1105 14-15 -0.0328877142162014 1105 16-17 -0.1231944477861262 1105 18-19 -0.033966175491599415 1105 20-21 -0.12162361196134697 1105 22-23 -0.0028692781722199356 1105 24-25 -0.09460593751287405 1105 26-27 -0.03229337927320586 1105 28-29 -0.1906337460428631 1105 30-31 -0.17052209203463775 1105 32-33 -0.13282999136378493 1105 34-35 -0.06142349363580735 1105 36-37 -0.044521145301843035 1105 38-39 -0.02613584807944136 1105 40-41 -0.06186442069282805 1105 42-43 -0.2753757822092595 1105 44-45 -0.1516677163874789 1105 46-47 -7.086337644039986E-4 1105 48-49 -0.17657945897803273 1105 50-51 -0.09967037281510471 1105 52-53 -0.03175955226843996 1105 54-55 0.01901315924784086 1105 56-57 -0.027926562923262566 1105 58-59 0.006260602661328107 1105 60-61 -0.20552690901432413 1105 62-63 -0.15217296928015145 1105 64-65 0.06457335435160161 1105 66-67 -0.22634031409974398 1105 68-69 -0.2159409919987425 1105 70-71 -0.33858756692492165 1105 72-73 -0.26356958736443303 1105 74-75 -0.11884091947897346 1105 76-77 -0.32237803971758794 1105 78-79 -0.14047952643113248 1105 80-81 -0.1436738431320954 1105 82-83 -0.2714407687887537 1105 84-85 -0.23756113115691235 1105 86-87 -0.17526213667691692 1105 88-89 -0.07718005975028674 1105 90-91 -0.1932503329048565 1105 92-93 -8.803773040320095E-4 1105 94-95 0.05669148046617423 1105 96-97 -0.006427713696520954 1105 98-99 -0.14997841750658836 1105 100-101 -0.17367095078088113 1106 1 -0.2321171321804698 1106 2 -0.0975763373689702 1106 3 -0.16081308198112865 1106 4 -0.0827345897452787 1106 5 -0.2144263815607701 1106 6 -0.18608396207996947 1106 7 -0.17584202500138701 1106 8 -0.11554777988550313 1106 9 -0.21316436793745908 1106 10-11 -0.2559413048861856 1106 12-13 -0.4130141896832811 1106 14-15 -0.18788169697080548 1106 16-17 -0.1666192697230926 1106 18-19 -0.20819714285066482 1106 20-21 -0.29941501265032855 1106 22-23 -0.27850710066844897 1106 24-25 -0.2226800549743615 1106 26-27 -0.048258934817738464 1106 28-29 -0.13604613417780342 1106 30-31 -0.2749858291687133 1106 32-33 -0.18938603116695418 1106 34-35 -0.1441093250360197 1106 36-37 -0.23163238216290694 1106 38-39 -0.21706150007638314 1106 40-41 -0.25926536778849396 1106 42-43 -0.478801420417831 1106 44-45 -0.1288857414293645 1106 46-47 -0.0598271045567742 1106 48-49 -0.2782207164334096 1106 50-51 0.010472794605512092 1106 52-53 -0.03642774337747312 1106 54-55 -0.020797178942309813 1106 56-57 0.11645919821140183 1106 58-59 0.05554686139074505 1106 60-61 0.26704636515034963 1106 62-63 0.2925684766898371 1106 64-65 0.11318067366050855 1106 66-67 0.349210310734108 1106 68-69 0.2743781922591211 1106 70-71 0.17438425736489904 1106 72-73 0.15814803560853719 1106 74-75 0.2240662318324098 1106 76-77 0.20497119546901033 1106 78-79 0.11132013183786782 1106 80-81 0.28512603906006007 1106 82-83 0.21906759192828318 1106 84-85 0.11107150360236062 1106 86-87 0.37095455475366634 1106 88-89 0.36970713261735355 1106 90-91 0.3627398321254063 1106 92-93 0.38849193189523845 1106 94-95 0.32760595836672124 1106 96-97 0.5249775088974324 1106 98-99 0.5264393855976728 1106 100-101 0.5187419149832557 1107 1 -0.11516243036937013 1107 2 -0.05551731688477446 1107 3 -0.03152445571572571 1107 4 -0.025462383991559534 1107 5 -0.15741592679025018 1107 6 -0.10512002045916802 1107 7 -0.11308068300409957 1107 8 -0.10510609691392858 1107 9 -0.19270198482563217 1107 10-11 -0.07241787490129781 1107 12-13 -0.0646590764868833 1107 14-15 -0.08733387397555248 1107 16-17 -0.15994189307869533 1107 18-19 -0.045699405906042045 1107 20-21 -0.1650822268127996 1107 22-23 -0.03265858032494151 1107 24-25 0.025355722022524674 1107 26-27 -0.10475352202552557 1107 28-29 -0.21111031770014677 1107 30-31 -0.07905213069275874 1107 32-33 -0.16702431748326063 1107 34-35 -0.12247996879074918 1107 36-37 -0.25348858926027873 1107 38-39 -0.08187802354344598 1107 40-41 -0.23927086455914548 1107 42-43 -0.05750775980852296 1107 44-45 -0.23723810624044006 1107 46-47 -0.2895795753769903 1107 48-49 -0.2603551212007673 1107 50-51 -0.3185596428850843 1107 52-53 -0.14842717188782473 1107 54-55 -0.3170742074578996 1107 56-57 -0.1371976811497504 1107 58-59 -0.14909769168035325 1107 60-61 -0.1832476684541149 1107 62-63 -0.011861004323399982 1107 64-65 -0.12121351459516205 1107 66-67 -0.25263863277348264 1107 68-69 -0.1704065284634737 1107 70-71 -0.45130202099418426 1107 72-73 -0.2800231102718307 1107 74-75 -0.27153253444795666 1107 76-77 -0.1826743762496683 1107 78-79 -0.17780509637627162 1107 80-81 -0.1342197579825921 1107 82-83 -0.14591307121783004 1107 84-85 -0.2757348290264261 1107 86-87 -0.13253491304114817 1107 88-89 -0.09151882173763681 1107 90-91 0.0022749187540718196 1107 92-93 0.016391096691478424 1107 94-95 -0.05932416066453783 1107 96-97 -4.4396471697538686E-4 1107 98-99 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66-67 0.23972607864404694 2316 68-69 0.44745091599001086 2316 70-71 0.570542979593931 2316 72-73 0.5366100792000488 2316 74-75 0.4836979002428663 2316 76-77 0.465819979374654 2316 78-79 0.3056858964487503 2316 80-81 0.23010448523676885 2316 82-83 0.11369578847653017 2316 84-85 0.23097682073576564 2316 86-87 0.18369903826310718 2316 88-89 0.27105126171407434 2316 90-91 0.2769628419314536 2316 92-93 0.33621291356820393 2316 94-95 0.5127343701390039 2316 96-97 0.591202494994274 2316 98-99 0.13592401043232627 2316 100-101 -0.2074707232235653 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 10.0 4 18.0 5 56.0 6 124.0 7 287.0 8 612.0 9 1284.0 10 2024.0 11 3186.0 12 4529.0 13 6044.0 14 7748.0 15 10490.0 16 13321.0 17 17399.0 18 16031.0 19 11623.0 20 10916.0 21 11536.0 22 12425.0 23 14107.0 24 16368.0 25 20097.0 26 24987.0 27 30651.0 28 38251.0 29 47262.0 30 58272.0 31 72430.0 32 90210.0 33 115191.0 34 150919.0 35 205700.0 36 293333.0 37 390472.0 38 246732.0 39 15710.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.3288716441005 20.636316360780345 15.523371559129714 22.511440435989442 2 16.227291137639146 21.45286728240454 38.796485131797354 23.523356448158957 3 19.63835177044193 24.788686556231056 30.090473270698514 25.4824884026285 4 13.650312851020068 17.6911054001361 36.70656074043618 31.952021008407648 5 13.690018545114116 37.543882025524404 34.33463344701888 14.431465982342603 6 31.79108101683468 36.98691769564539 17.134829454620025 14.087171832899909 7 28.27718202491586 32.80997654510043 20.709431644628175 18.203409785355532 8 26.214395533876978 35.974092487188564 19.449304667820876 18.362207311113583 9 25.78733068143676 17.043621624213536 20.214062941564755 36.95498475278495 10-11 24.789477228138942 27.32585068645625 27.188069730120723 20.696602355284085 12-13 25.507158321753092 24.299854711371488 28.08429699281107 22.108689974064347 14-15 22.523079968046652 25.909731793886625 26.122320514926358 25.444867723140362 16-17 21.954561360731052 29.13648935551568 26.827931428851258 22.08101785490201 18-19 21.900923198721866 27.65094436832456 29.170411730918538 21.277720702035037 20-21 22.548075402554023 26.692190916148988 29.538380234630612 21.221353446666374 22-23 22.3193541714437 26.581943030640943 29.31693545157727 21.78176734633809 24-25 22.115803987763393 26.811171695623276 29.41703688173573 21.655987434877602 26-27 21.948056751155747 27.06004782400765 29.259901163717522 21.73199426111908 28-29 21.940557300179357 27.152595408476625 29.28799964087276 21.618847650471253 30-31 22.070207736976172 26.910338744895974 29.378202092035586 21.641251426092268 32-33 21.889598736557303 26.96520227427847 29.245959156439795 21.899239832724433 34-35 22.16755817049743 27.073881403294703 29.186607752257498 21.57195267395037 36-37 22.23220341014852 26.8848312894428 29.148222936208732 21.734742364199946 38-39 22.03560891105271 27.082712696495324 29.20429596317396 21.67738242927801 40-41 22.281491441869292 27.0169530259269 29.01449633179246 21.687059200411355 42-43 22.088696906745245 26.978510674769506 28.936946211895503 21.995846206589746 44-45 22.17965345606605 27.161652912797795 28.873993646567016 21.78469998456914 46-47 22.295238930848345 27.34123053034191 28.4226401504215 21.940890388388244 48-49 22.314342584364695 27.421726031673803 28.29891274961002 21.96501863435148 50-51 22.458015321691242 27.46105558270479 28.226502506174896 21.854426589429075 52-53 22.467251475561447 27.623918212793548 27.911570120827573 21.997260190817432 54-55 22.40993736858268 27.492401898020667 28.029038573566666 22.06862215982999 56-57 22.398663917508944 27.786659375481413 27.933290756076186 21.881385950933453 58-59 22.42539372910458 27.516989243801387 28.09313400919628 21.964483017897752 60-61 22.385018450711552 27.490769543114062 28.270652605289442 21.85355940088494 62-63 22.232393266944097 27.572055716353848 28.321791223847885 21.873759792854162 64-65 22.196047864726747 27.408641686875562 28.32735143274851 22.067959015649183 66-67 22.24348817919992 27.52747202296723 28.127438967780378 22.10160083005247 68-69 22.127693003012716 27.537062108043532 28.18615273332728 22.149092155616472 70-71 22.282154586050098 27.42501624703243 28.114329117436714 22.17850004948076 72-73 22.238565608934692 27.428229945754808 28.031640139199066 22.301564306111434 74-75 22.16347728323092 27.750722061990718 27.71695271986035 22.36884793491801 76-77 22.222420598686565 27.473272738958904 27.956577319040704 22.347729343313823 78-79 22.260831950082586 27.475695765773395 27.89556805440639 22.36790422973763 80-81 22.162355039232633 27.729603470386532 27.779568833855855 22.32847265652498 82-83 22.255286045859158 27.629020328002653 27.768204657331598 22.34748896880659 84-85 22.246126598369585 27.556332851618354 27.767442358713236 22.430098191298832 86-87 22.13215647346046 27.52989504978172 27.881361465609857 22.456587011147963 88-89 22.152764954156332 27.503012204913592 27.826499037420717 22.51772380350936 90-91 22.219237860278806 27.508675055529537 27.794867713693066 22.477219370498588 92-93 22.08661469540084 27.42948323584895 27.838065738448027 22.645836330302185 94-95 22.039775387964852 27.487632361027934 27.738734455645346 22.733857795361867 96-97 22.023196783444657 27.441951929188445 27.703562308517114 22.83128897884978 98-99 21.885280480980487 27.39274887021515 27.70060535757008 23.02136529123428 100-101 21.931767223105663 27.377747439855156 27.847815184912527 22.842670152126658 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 358.0 1 266.5 2 207.0 3 277.5 4 269.5 5 262.0 6 483.0 7 978.5 8 1611.5 9 2054.5 10 2023.0 11 1837.0 12 1942.0 13 2194.5 14 2369.0 15 2512.0 16 2674.5 17 2794.5 18 2914.5 19 3054.0 20 3401.0 21 3994.5 22 4698.5 23 5630.5 24 6808.5 25 8511.5 26 10869.5 27 13796.0 28 16427.0 29 19611.0 30 23780.0 31 27079.0 32 30535.0 33 34517.5 34 38631.0 35 43019.5 36 48777.5 37 54027.5 38 56821.5 39 59267.5 40 61372.5 41 64448.5 42 67557.0 43 70153.0 44 73047.5 45 75144.0 46 76846.0 47 78104.0 48 77755.5 49 77271.0 50 75830.0 51 71371.5 52 67738.5 53 65524.5 54 63263.0 55 58568.5 56 51574.5 57 45036.5 58 39514.5 59 34526.0 60 29006.5 61 23589.5 62 18538.5 63 14028.5 64 10420.0 65 7584.5 66 5504.0 67 4279.0 68 3259.5 69 2321.5 70 1722.5 71 1278.0 72 928.0 73 706.0 74 527.0 75 355.5 76 240.0 77 138.0 78 74.0 79 42.5 80 24.0 81 14.5 82 7.5 83 3.0 84 3.5 85 4.0 86 1.5 87 1.0 88 0.5 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.043920549205808325 2 0.0 3 0.0 4 0.0 5 0.013926027796963615 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.006223353081426964 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0011987606345371612 24-25 5.611219991450541E-4 26-27 0.002321004632827269 28-29 0.0025505545415684277 30-31 9.437051803803183E-4 32-33 0.0 34-35 0.0 36-37 1.7853881790978995E-4 38-39 3.060665449882113E-4 40-41 0.0 42-43 0.01132446216456382 44-45 2.550554541568428E-5 46-47 0.0 48-49 0.0 50-51 0.0 52-53 1.275277270784214E-4 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 4.080887266509485E-4 84-85 5.101109083136856E-5 86-87 0.0 88-89 0.0 90-91 0.007141552716391598 92-93 0.0022444879965802162 94-95 0.0 96-97 0.0 98-99 0.0014793216341096882 100-101 0.01096738452874424 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1960358.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.18974668058613 #Duplication Level Percentage of deduplicated Percentage of total 1 79.9002506336745 48.89076095989885 2 11.75599746278804 14.38693013451227 3 3.5016292201070645 6.427914148430689 4 1.5836572893395982 3.876143534542148 5 0.8229570310249336 2.517826612871147 6 0.538131640985482 1.9756883275625858 7 0.34082174876469407 1.4598357529102202 8 0.24022422898606266 1.1759407774557031 9 0.18726515097229765 1.0312836435086952 >10 1.0341204558799189 11.487425363188617 >50 0.06692823596114739 2.8010494129795593 >100 0.02735018959832325 2.8527696207424276 >500 2.5001696919926066E-4 0.10030700115644398 >1k 2.500169691992546E-4 0.3491111997544497 >5k 1.6667797946616974E-4 0.6670135104861528 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6903 0.35212956000893714 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6177 0.31509550806536357 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3943 0.2011367311480862 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.5505545415684277E-5 0.0 12-13 0.0 0.0 0.0 5.101109083136855E-5 0.0 14-15 0.0 0.0 0.0 7.651663624705283E-5 2.5505545415684277E-5 16-17 0.0 0.0 0.0 1.5303327249410566E-4 5.101109083136855E-5 18-19 0.0 0.0 0.0 2.040443633254742E-4 5.101109083136855E-5 20-21 0.0 0.0 0.0 2.295499087411585E-4 5.101109083136855E-5 22-23 0.0 0.0 0.0 3.570776358195799E-4 5.101109083136855E-5 24-25 0.0 0.0 0.0 6.376386353921069E-4 5.101109083136855E-5 26-27 0.0 0.0 0.0 7.396608170548441E-4 5.101109083136855E-5 28-29 0.0 0.0 0.0 0.0015303327249410565 5.101109083136855E-5 30-31 0.0 0.0 0.0 0.003851337357768326 5.101109083136855E-5 32-33 0.0 0.0 0.0 0.007575146988458231 5.101109083136855E-5 34-35 0.0 0.0 0.0 0.01180906752746182 5.101109083136855E-5 36-37 0.0 0.0 0.0 0.017624331882237835 5.101109083136855E-5 38-39 0.0 0.0 0.0 0.0256330731427627 5.101109083136855E-5 40-41 0.0 0.0 0.0 0.03716157967065199 5.101109083136855E-5 42-43 0.0 0.0 0.0 0.049888846833078454 5.101109083136855E-5 44-45 0.0 0.0 0.0 0.06549824062747722 5.101109083136855E-5 46-47 0.0 0.0 0.0 0.08682087659498927 5.101109083136855E-5 48-49 0.0 0.0 0.0 0.10834755692582682 5.101109083136855E-5 50-51 0.0 0.0 0.0 0.1346437742493973 5.101109083136855E-5 52-53 0.0 0.0 0.0 0.17318265337249625 5.101109083136855E-5 54-55 0.0 0.0 0.0 0.22386217211346093 5.101109083136855E-5 56-57 0.0 0.0 0.0 0.2742356243094374 5.101109083136855E-5 58-59 0.0 2.5505545415684277E-5 0.0 0.3430495858409535 5.101109083136855E-5 60-61 0.0 5.101109083136855E-5 0.0 0.42135161026710427 5.101109083136855E-5 62-63 0.0 5.101109083136855E-5 0.0 0.49223152097729084 5.101109083136855E-5 64-65 0.0 5.101109083136855E-5 0.0 0.5693857958597358 5.101109083136855E-5 66-67 0.0 5.101109083136855E-5 0.0 0.6825028897782957 5.101109083136855E-5 68-69 0.0 5.101109083136855E-5 0.0 0.8064598404985213 5.101109083136855E-5 70-71 0.0 5.101109083136855E-5 0.0 0.9174344686021635 5.101109083136855E-5 72-73 0.0 5.101109083136855E-5 0.0 1.056261152299733 5.101109083136855E-5 74-75 0.0 5.101109083136855E-5 0.0 1.2187059710522261 5.101109083136855E-5 76-77 0.0 5.101109083136855E-5 0.0 1.3918121077884753 5.101109083136855E-5 78-79 0.0 5.101109083136855E-5 0.0 1.592260189210338 5.101109083136855E-5 80-81 0.0 5.101109083136855E-5 0.0 1.8211469537706888 5.101109083136855E-5 82-83 0.0 5.101109083136855E-5 0.0 2.079237567831998 5.101109083136855E-5 84-85 0.0 5.101109083136855E-5 0.0 2.3417661467956363 5.101109083136855E-5 86-87 0.0 5.101109083136855E-5 0.0 2.61921546982745 5.101109083136855E-5 88-89 0.0 5.101109083136855E-5 0.0 2.9353567052548564 5.101109083136855E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3490 0.0 57.743317 1 GTATCAA 6285 0.0 42.046593 1 ATCAACG 8080 0.0 32.15833 3 TCAACGC 8160 0.0 31.95948 4 TATCAAC 8295 0.0 31.611143 2 CAACGCA 8345 0.0 31.19405 5 TGGTATC 1440 0.0 30.348892 2 AACGCAG 8725 0.0 29.831648 6 GTGGTAT 1835 0.0 25.642504 1 ACGCAGA 10415 0.0 24.586823 7 CGCAGAG 10480 0.0 24.252995 8 GCAGAGT 11350 0.0 21.975376 9 GAGTACT 6975 0.0 21.217134 12-13 GTACATG 6890 0.0 21.039816 1 TACATGG 6885 0.0 20.698307 2 GTACTTT 7285 0.0 20.015709 14-15 CAGAGTA 11005 0.0 19.901407 10-11 ACATGGG 6860 0.0 19.873543 3 AGTACTT 7205 0.0 19.352943 12-13 AGAGTAC 10550 0.0 19.341208 10-11 >>END_MODULE