FastQCFastQC Report
Thu 26 May 2016
SRR937477_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937477_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2052801
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65990.3214632105109068No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52060.2536047088831309No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33840.1648479321668296No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN31080.15140288805393218No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30800.054.8074951
GTATCAA58850.043.2284471
ATCAACG75300.033.5174373
TCAACGC75500.033.3043674
TATCAAC77150.032.777722
CAACGCA77800.032.3824735
AACGCAG80750.031.2988346
TGGTATC14100.029.3294012
ACGCAGA97150.025.917447
CGCAGAG96800.025.9123618
GTACATG73200.024.4902151
TACATGG72350.023.6519572
GCAGAGT104500.023.5012459
GTGGTAT19050.023.4636291
ACATGGG72800.022.0676753
GAGTACT61550.021.22370712-13
GTGTAGG8750.021.194291
AGAGTAC96950.020.48071510-11
AGTACTT64350.020.18949112-13
GTACTTT65200.019.92336514-15
Produced by FastQC (version 0.11.5)