##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937477_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2052801 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.519359645674374 31.0 31.0 34.0 30.0 34.0 2 31.612685788831943 33.0 31.0 34.0 30.0 34.0 3 31.664969473417052 33.0 31.0 34.0 30.0 34.0 4 35.31581629198349 37.0 35.0 37.0 33.0 37.0 5 35.2945521752961 37.0 35.0 37.0 33.0 37.0 6 35.353326016501356 37.0 35.0 37.0 33.0 37.0 7 35.28841616893211 37.0 35.0 37.0 33.0 37.0 8 35.269651563887585 37.0 35.0 37.0 33.0 37.0 9 36.86646148360216 39.0 37.0 39.0 33.0 39.0 10-11 36.7637123617925 39.0 37.0 39.0 33.0 39.0 12-13 36.63615956929094 39.0 37.0 39.0 32.0 39.0 14-15 37.76187389815184 40.0 37.5 41.0 32.5 41.0 16-17 37.68914936226161 40.0 37.0 41.0 32.0 41.0 18-19 37.62272402439399 40.0 37.0 41.0 32.0 41.0 20-21 37.544575679766325 40.0 37.0 41.0 32.0 41.0 22-23 37.39733442257676 40.0 37.0 41.0 32.0 41.0 24-25 37.24197620714331 39.0 37.0 41.0 31.5 41.0 26-27 37.06790940768248 39.0 36.0 41.0 31.0 41.0 28-29 36.878053206326385 39.0 36.0 40.5 31.0 41.0 30-31 36.8935308390828 39.0 36.0 41.0 31.0 41.0 32-33 36.75271543612849 39.0 36.0 41.0 30.0 41.0 34-35 36.548658394067424 39.0 35.5 40.0 30.0 41.0 36-37 36.566830637748126 39.0 36.0 41.0 30.0 41.0 38-39 36.54780175964451 39.0 36.0 41.0 30.0 41.0 40-41 36.38767834777945 39.0 35.5 41.0 30.0 41.0 42-43 36.159612402760914 39.0 35.0 41.0 30.0 41.0 44-45 35.95947147336736 39.0 35.0 40.5 29.0 41.0 46-47 35.66447112993417 39.0 35.0 40.0 27.5 41.0 48-49 35.39136136430175 39.0 35.0 40.0 27.0 41.0 50-51 34.48719822330562 38.0 34.0 39.5 25.0 40.5 52-53 34.63849174859132 38.0 34.0 39.5 26.0 40.5 54-55 34.99241231858325 38.0 34.0 40.0 26.0 41.0 56-57 34.760245391540636 38.0 34.0 40.0 25.5 41.0 58-59 34.507760859430604 38.0 34.0 40.0 25.0 41.0 60-61 34.2249989161151 38.0 34.0 40.0 23.5 41.0 62-63 33.89861145819785 37.0 33.0 40.0 23.0 41.0 64-65 33.53071924653193 37.0 33.0 40.0 22.0 41.0 66-67 33.13388292386841 36.0 32.5 39.0 21.5 41.0 68-69 32.619337919262506 36.0 32.0 39.0 19.5 40.0 70-71 32.185533814529514 35.0 31.0 38.5 18.0 40.0 72-73 31.757351540651044 35.0 31.0 38.0 17.5 40.0 74-75 31.283968830880344 35.0 31.0 37.0 13.5 39.0 76-77 30.82558343453652 35.0 30.0 36.5 9.5 39.0 78-79 30.337507142679684 34.0 30.0 36.0 7.0 38.5 80-81 29.873602458299658 34.0 29.5 36.0 4.5 37.5 82-83 29.413359843452923 34.0 29.0 35.0 2.0 37.0 84-85 28.91900456985358 34.0 29.0 35.0 2.0 36.5 86-87 28.451189131338108 34.0 28.5 35.0 2.0 36.0 88-89 28.042547475376328 34.0 27.0 35.0 2.0 36.0 90-91 27.57564371802235 33.0 26.5 35.0 2.0 35.0 92-93 27.079713035993258 33.0 25.5 35.0 2.0 35.0 94-95 26.484441745692834 32.5 25.0 35.0 2.0 35.0 96-97 25.801301490012914 32.0 23.5 34.5 2.0 35.0 98-99 25.02735822907335 32.0 19.0 34.0 2.0 35.0 100-101 23.137975624524735 30.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.09127003385259158 1101 2 0.08478636002087114 1101 3 0.19001020735350593 1101 4 0.08650786822786927 1101 5 0.12269954168479558 1101 6 0.01886374403154889 1101 7 0.17937459033515069 1101 8 -0.018902638366753877 1101 9 0.1558485100066207 1101 10-11 0.13146327629390697 1101 12-13 0.2172956464936746 1101 14-15 0.28131100775714657 1101 16-17 0.3109564156544238 1101 18-19 0.3767620112544776 1101 20-21 0.4027951935657583 1101 22-23 0.20362000134502978 1101 24-25 0.1767470968519902 1101 26-27 0.4087918657194862 1101 28-29 0.3750924181630211 1101 30-31 0.292301824535798 1101 32-33 0.40012419645427144 1101 34-35 0.18933946459344497 1101 36-37 0.35232865691214954 1101 38-39 0.42024437612849397 1101 40-41 0.39297891044008537 1101 42-43 0.5225578943482958 1101 44-45 0.5113104056422273 1101 46-47 0.6201186959333853 1101 48-49 0.6059178109544803 1101 50-51 0.5992119165846788 1101 52-53 0.577565055481692 1101 54-55 0.6843691668943634 1101 56-57 0.7443771391030012 1101 58-59 0.6697757880126076 1101 60-61 0.6485387724899496 1101 62-63 0.7158477676639663 1101 64-65 0.6093103042018058 1101 66-67 0.7968162517322384 1101 68-69 0.7342733585045664 1101 70-71 0.8271588388250777 1101 72-73 0.9420717587337748 1101 74-75 0.8971831845437457 1101 76-77 0.9511081099471745 1101 78-79 0.9279776212888144 1101 80-81 1.1182006725344777 1101 82-83 1.092987185731193 1101 84-85 0.8506543954547219 1101 86-87 1.1158248439090066 1101 88-89 1.0320015762512504 1101 90-91 0.9548217357535407 1101 92-93 0.9626745524633193 1101 94-95 0.9133908739556809 1101 96-97 0.9141820632797923 1101 98-99 1.1808533057469859 1101 100-101 1.8093060033892812 1102 1 0.013899825323186121 1102 2 -0.17816391531653508 1102 3 -0.02537400260475664 1102 4 -0.2033462016870473 1102 5 -0.1101272576290242 1102 6 -0.04439633069777216 1102 7 -0.09914451896641197 1102 8 -0.11669891213191619 1102 9 0.06360161376519358 1102 10-11 -0.06009575601122208 1102 12-13 0.08049009134180807 1102 14-15 0.03277256602793699 1102 16-17 0.07228144555797655 1102 18-19 0.11258052167126209 1102 20-21 0.13725431091652496 1102 22-23 0.16849890772294884 1102 24-25 0.25986025908257915 1102 26-27 0.1535308663199828 1102 28-29 0.21474827111023842 1102 30-31 0.07823854430898791 1102 32-33 -0.05681953992248623 1102 34-35 0.07702303981949399 1102 36-37 0.05981276922973677 1102 38-39 0.2663801867718476 1102 40-41 0.18198678699467763 1102 42-43 0.11124194256678521 1102 44-45 -0.027928977557031942 1102 46-47 -0.05491573214201395 1102 48-49 0.030855208728723937 1102 50-51 -0.07740667769574117 1102 52-53 0.015531544519440388 1102 54-55 0.046704823335595336 1102 56-57 0.05433823203525634 1102 58-59 -0.08222173793270571 1102 60-61 -0.07004244783055213 1102 62-63 0.07650586078554511 1102 64-65 0.06585080531801424 1102 66-67 0.16918483162523756 1102 68-69 0.25836409787061854 1102 70-71 0.12355962018300914 1102 72-73 0.11222541248896789 1102 74-75 -0.007447994707209915 1102 76-77 -0.09550644810523679 1102 78-79 0.013163928186695983 1102 80-81 -0.006764473096232848 1102 82-83 0.17594881986291355 1102 84-85 0.2091328673229107 1102 86-87 0.15652205530756547 1102 88-89 0.09664013038640462 1102 90-91 0.2885272042708671 1102 92-93 0.1886979920387759 1102 94-95 0.02396404739780067 1102 96-97 0.27109913691179344 1102 98-99 0.35336000485849084 1102 100-101 0.5228402916596728 1103 1 -0.04635158343277368 1103 2 -0.0927716309645632 1103 3 -0.07955692414788729 1103 4 -0.09411343185609411 1103 5 -0.30475231181194573 1103 6 -0.20607816858246508 1103 7 -0.11171495676441623 1103 8 -0.15180944529186036 1103 9 -0.08377593283211837 1103 10-11 -0.1381191630333376 1103 12-13 -0.03307731221259047 1103 14-15 0.012183055841546775 1103 16-17 -0.06555990684774571 1103 18-19 -0.09028033658621126 1103 20-21 -0.0109901624254789 1103 22-23 -0.012038586858750477 1103 24-25 -0.05049951551646359 1103 26-27 -0.13580593472033087 1103 28-29 -0.3281646027519258 1103 30-31 -0.2663648367053142 1103 32-33 -0.19877879436548085 1103 34-35 -0.08270821288964925 1103 36-37 -0.13134457797442423 1103 38-39 -0.023823541013150873 1103 40-41 0.04652948313685812 1103 42-43 0.005476879714599647 1103 44-45 -0.08818038631298464 1103 46-47 -0.05751651237608968 1103 48-49 -0.07122541545852812 1103 50-51 -0.05876775268490064 1103 52-53 -0.07441210524215336 1103 54-55 0.02178067942575268 1103 56-57 0.07861218088658006 1103 58-59 0.012273277238513458 1103 60-61 0.053928076660135105 1103 62-63 0.14174209832346918 1103 64-65 0.18808747631931055 1103 66-67 0.24287360492388643 1103 68-69 0.13656089024166818 1103 70-71 0.22347292750853853 1103 72-73 0.33567578093283146 1103 74-75 0.305295828439732 1103 76-77 0.23284205644612754 1103 78-79 0.38290818479701016 1103 80-81 0.299477399465534 1103 82-83 0.39961591999295365 1103 84-85 0.31316407668571955 1103 86-87 0.3747708632832918 1103 88-89 0.4041823930652093 1103 90-91 0.4523763590172898 1103 92-93 0.6780781394163213 1103 94-95 0.6325466221702349 1103 96-97 0.792772305529045 1103 98-99 0.8693981496352627 1103 100-101 1.028908778872502 1104 1 0.0638032409813718 1104 2 0.0747638983742327 1104 3 0.14326039602335428 1104 4 0.006073763108574326 1104 5 0.087474515459256 1104 6 0.03279536502163438 1104 7 -0.028621816714078818 1104 8 0.02786029286196623 1104 9 0.14039764279083045 1104 10-11 0.05794050556907848 1104 12-13 0.11701256091409817 1104 14-15 0.26647772649143064 1104 16-17 0.14113178306387653 1104 18-19 0.08199259157041894 1104 20-21 0.06526878501630762 1104 22-23 0.15358793365092538 1104 24-25 0.1658374258324926 1104 26-27 0.096692866387464 1104 28-29 0.10611757471288286 1104 30-31 0.0010262908839209217 1104 32-33 -0.06276265278687276 1104 34-35 0.037227931004700565 1104 36-37 0.12462559178059962 1104 38-39 0.13587314156315955 1104 40-41 -0.06990318521462768 1104 42-43 -0.05127252849081998 1104 44-45 -0.02323343164669467 1104 46-47 -0.08114948487098417 1104 48-49 0.061035303449038736 1104 50-51 -0.14143544963371113 1104 52-53 -0.095915465366879 1104 54-55 -0.19182605111176798 1104 56-57 -0.05929213525442378 1104 58-59 -0.17568720845434171 1104 60-61 -0.04670594879491574 1104 62-63 0.18180971197209317 1104 64-65 0.08870046469619552 1104 66-67 0.17071910021432757 1104 68-69 0.2732100800929089 1104 70-71 0.10370871240819213 1104 72-73 0.08991808935483903 1104 74-75 0.15422158230370542 1104 76-77 0.15532798271458148 1104 78-79 0.22217965289560127 1104 80-81 0.2446478781577035 1104 82-83 0.30577653891448975 1104 84-85 0.3325594883901921 1104 86-87 0.31526146785867937 1104 88-89 0.32009667536067 1104 90-91 0.3835564432282901 1104 92-93 0.42876018509056735 1104 94-95 0.5257899957210732 1104 96-97 0.45682334985127326 1104 98-99 0.4248401615100512 1104 100-101 0.7103273970001176 1105 1 0.11281583473648027 1105 2 0.14132018023141413 1105 3 0.152025449670262 1105 4 0.16300452941564458 1105 5 0.09417238901252034 1105 6 0.12081964533386724 1105 7 0.04652242505038373 1105 8 0.14645044561709142 1105 9 0.04588608199199484 1105 10-11 0.1920921821922832 1105 12-13 0.20151162555855962 1105 14-15 0.17028286977892293 1105 16-17 0.10074055800435389 1105 18-19 0.2413653682789274 1105 20-21 0.16794440342746242 1105 22-23 0.26920579071165207 1105 24-25 0.2870865197662411 1105 26-27 0.14982234635658642 1105 28-29 -0.04694347609038374 1105 30-31 0.08180783561574856 1105 32-33 0.011034916031157138 1105 34-35 0.17244615376942818 1105 36-37 0.22630283970767096 1105 38-39 0.04917434813648214 1105 40-41 0.17279409730186046 1105 42-43 0.2951209249077138 1105 44-45 0.16931789339655268 1105 46-47 0.013185072726948022 1105 48-49 0.0826861901219118 1105 50-51 0.0306148305021523 1105 52-53 -0.058653801761316515 1105 54-55 -0.018971243894959855 1105 56-57 0.19777364239442363 1105 58-59 0.1804725663142932 1105 60-61 0.1988465628488214 1105 62-63 0.15567797342401946 1105 64-65 0.11773188688385972 1105 66-67 0.06986303849299702 1105 68-69 0.2020144130588335 1105 70-71 0.2171863246268657 1105 72-73 0.1515103141791414 1105 74-75 0.0211190683874527 1105 76-77 0.14699416499908935 1105 78-79 0.2515790244482261 1105 80-81 0.25005620869791656 1105 82-83 0.19270109969756888 1105 84-85 0.20417322702554586 1105 86-87 0.12026143254857757 1105 88-89 0.15860601687436215 1105 90-91 0.20002123570720087 1105 92-93 0.2137591502329812 1105 94-95 0.2511719287915959 1105 96-97 0.26541761125358576 1105 98-99 0.3723631941081571 1105 100-101 0.5972999433056962 1106 1 0.017388082804234983 1106 2 0.056298477835124316 1106 3 -0.025307403754748492 1106 4 -0.05801381063229627 1106 5 -0.025007647997234983 1106 6 -0.11598876248056911 1106 7 0.004633043919078261 1106 8 -0.10482547886986282 1106 9 0.08584125317238289 1106 10-11 -0.0877076392250018 1106 12-13 -0.23490883767257031 1106 14-15 -0.2094233807279906 1106 16-17 -0.3100679753935651 1106 18-19 -0.0899201319488796 1106 20-21 -0.07926435407717491 1106 22-23 0.031837116092106044 1106 24-25 -0.0862629076889263 1106 26-27 0.20643470937807962 1106 28-29 0.2894033937225231 1106 30-31 0.10685968552405001 1106 32-33 0.31927986437933953 1106 34-35 0.2842242402732822 1106 36-37 0.015057866317533808 1106 38-39 -0.0945590358787527 1106 40-41 0.15408757731371736 1106 42-43 0.13727229337527547 1106 44-45 0.08950220585125379 1106 46-47 0.1736149868629795 1106 48-49 0.3188555611317838 1106 50-51 0.1794964833901318 1106 52-53 0.3351684437835516 1106 54-55 0.3347595013720124 1106 56-57 0.2656350714821727 1106 58-59 0.16193564009720518 1106 60-61 0.14010441975914745 1106 62-63 0.3970221502934095 1106 64-65 0.16337587788221697 1106 66-67 0.23195814854861396 1106 68-69 0.1697378685034181 1106 70-71 -0.006958427534492273 1106 72-73 0.3106489796527683 1106 74-75 0.21666236834024133 1106 76-77 0.12184289483051103 1106 78-79 0.08375033676949784 1106 80-81 0.15331870696460115 1106 82-83 0.018718353971610924 1106 84-85 0.12463785976299135 1106 86-87 0.14591970866854354 1106 88-89 0.2136582106422047 1106 90-91 0.4008815573429594 1106 92-93 0.42355113015756274 1106 94-95 0.5440774709478404 1106 96-97 0.5701504267792927 1106 98-99 0.6134720888202345 1106 100-101 0.8260849305473847 1107 1 0.034932928236514726 1107 2 -0.14674703762164754 1107 3 -0.06813721753414725 1107 4 -0.04890073822344476 1107 5 0.09976939557746078 1107 6 0.0545939277832872 1107 7 -0.04890219012740715 1107 8 0.06962061881508674 1107 9 0.08864685365294633 1107 10-11 0.007638416872218556 1107 12-13 0.1930716623043267 1107 14-15 0.12291588270715437 1107 16-17 0.022836141791408693 1107 18-19 0.1392979263663605 1107 20-21 0.021176013743165356 1107 22-23 0.1615727105655651 1107 24-25 0.024460811178038 1107 26-27 0.13117127423183206 1107 28-29 0.10505858525711176 1107 30-31 0.22731252966249116 1107 32-33 0.13715066517413277 1107 34-35 0.20374108330271667 1107 36-37 0.1229084277518524 1107 38-39 0.2151849685572671 1107 40-41 0.2675069202276319 1107 42-43 0.17077434965732863 1107 44-45 0.18239319979793578 1107 46-47 0.22301787952042673 1107 48-49 0.1087004851186606 1107 50-51 0.005615793958043014 1107 52-53 0.007944840438049994 1107 54-55 0.11578182011884763 1107 56-57 0.15410991090734427 1107 58-59 0.06813100999693944 1107 60-61 0.029237546694126593 1107 62-63 0.20501032089550364 1107 64-65 0.26940036843256365 1107 66-67 0.23932148665683428 1107 68-69 0.20052101161140712 1107 70-71 0.18425316157875926 1107 72-73 0.24458687531377876 1107 74-75 0.17165049273375743 1107 76-77 0.1047653106153632 1107 78-79 0.30503881696912316 1107 80-81 0.38277418499242444 1107 82-83 0.36340621497618386 1107 84-85 0.42138135590457537 1107 86-87 0.4233292553623187 1107 88-89 0.5443487517447423 1107 90-91 0.336183992535414 1107 92-93 0.494422993749815 1107 94-95 0.4832359201877061 1107 96-97 0.6176001749424991 1107 98-99 0.8394594276677907 1107 100-101 1.103808603205767 1108 1 -0.10393331832423769 1108 2 -0.2747628359708081 1108 3 -0.11630354397419396 1108 4 -0.19528327128459466 1108 5 -0.04393345345067701 1108 6 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2316 68-69 -0.6411128302748601 2316 70-71 -0.7392003751757485 2316 72-73 -1.27130114570965 2316 74-75 -1.1961407329031495 2316 76-77 -0.995230017510778 2316 78-79 -0.8989217640951921 2316 80-81 -0.9389203912540189 2316 82-83 -0.9491866750157847 2316 84-85 -1.238386802355155 2316 86-87 -1.4019959508571098 2316 88-89 -1.3919265968208485 2316 90-91 -1.455867982061271 2316 92-93 -1.2151457338945377 2316 94-95 -1.188411061343789 2316 96-97 -1.2693835464970213 2316 98-99 -1.4291724432716926 2316 100-101 -1.391216727110315 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8674.0 3 1789.0 4 1633.0 5 1805.0 6 2100.0 7 2528.0 8 3036.0 9 3815.0 10 4807.0 11 5915.0 12 7612.0 13 9302.0 14 10639.0 15 12591.0 16 15039.0 17 18266.0 18 16280.0 19 13068.0 20 13343.0 21 14192.0 22 15355.0 23 17364.0 24 19464.0 25 22289.0 26 25708.0 27 30679.0 28 36843.0 29 44698.0 30 54381.0 31 67452.0 32 85809.0 33 110803.0 34 148299.0 35 202992.0 36 286697.0 37 384243.0 38 296728.0 39 36552.0 40 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.90851326417146 19.222845412221858 14.67492698901475 23.193714334591935 2 15.220341997437803 20.17924621411631 40.45435490222712 24.146056886218766 3 19.1915381804039 24.28893627250749 29.51947864121502 27.000046905873592 4 12.67395533961418 16.57912506608373 37.32438340582592 33.42253618847617 5 13.848014788933313 37.17546616015352 34.25920497758601 14.71731407332716 6 31.940739496717296 36.12203609606349 17.66420350024801 14.273020906971205 7 28.48414243780113 32.32851978508946 20.87990477064686 18.30743300646255 8 26.232090249616004 35.13963087336794 19.880469840141473 18.74780903687458 9 25.926394260039242 16.95394624743133 20.464868079231067 36.65479141329836 10-11 24.717560054803457 26.869928702773276 27.504411522006826 20.908099720416438 12-13 25.392463921248186 23.981319833041553 28.203546341174025 22.42266990453623 14-15 22.556640758010644 25.52981830581652 26.23387502871123 25.6796659074616 16-17 22.070748966258705 28.788419558595272 26.876492510532714 22.264338964613312 18-19 21.97495528252074 27.421383156546327 29.10888218018215 21.494779380750785 20-21 22.568235475612543 26.571632359345188 29.361173003805995 21.498959161236275 22-23 22.276452413068558 26.421925367866205 29.182636705238856 22.11898551382638 24-25 22.010384865842216 26.657263304236444 29.304906182788088 22.027445647133252 26-27 21.936481254956945 26.893587561939725 29.09828652249725 22.07164466060608 28-29 21.90771239238979 27.04182938695442 29.151753522154777 21.89870469850101 30-31 22.11384261360349 26.768719000224355 29.170653085946263 21.946785300225894 32-33 21.847412363460233 26.80325993841536 29.139550763631515 22.209776934492893 34-35 22.19125382934837 26.92497528468383 29.02642402938987 21.857346856577937 36-37 22.145199239300744 26.743324048753685 29.023078498431808 22.088398213513763 38-39 21.93128491579206 26.98864267341017 29.04637556718904 22.033696843608734 40-41 22.260275477145022 26.904003776254022 28.8809235225577 21.954797224043258 42-43 22.002994235583955 26.86669891628588 28.81954190245743 22.310764945672734 44-45 22.041839406728666 27.072801407454655 28.81271278975841 22.072646396058268 46-47 22.121232831573064 27.257274384841256 28.33750314261236 22.28398964097332 48-49 22.173994872294355 27.238647756207694 28.305955544749768 22.281401826748187 50-51 22.30238870288985 27.371796968798872 28.213357850377353 22.11245647793393 52-53 22.306953469214758 27.449750062624727 27.960562777813315 22.282733690347197 54-55 22.202134864323664 27.320936191863375 28.134999089355023 22.341929854457938 56-57 22.16435442232289 27.6177301949353 28.04284701114983 22.175068371591973 58-59 22.235751873782178 27.30798841872672 28.239565934072637 22.216693773418463 60-61 22.23210333057888 27.391554971366205 28.241685511003254 22.13465618705166 62-63 22.098398518931408 27.388982087484763 28.439138008708554 22.073481384875276 64-65 22.041589682404688 27.255125376461276 28.348641518081248 22.354643423052792 66-67 22.036929373281414 27.446195105925675 28.126722247199126 22.39015327359378 68-69 22.027100636077172 27.438271424164682 28.216284786080493 22.31834315367765 70-71 22.101865855869953 27.279066151962393 28.227137951207226 22.391930040960432 72-73 22.047752845328418 27.315518954658057 28.167378319142998 22.469349880870524 74-75 22.016284132733254 27.613222559576688 27.849625601624506 22.520867706065548 76-77 22.13305884595698 27.303813247693533 27.949529016080405 22.61359889026908 78-79 22.157529047246413 27.285080154827522 28.04074059067413 22.51665020725194 80-81 22.042249344788363 27.53463000035875 27.878080727150085 22.545039927702803 82-83 22.11691107552917 27.52025892368976 27.851504530188237 22.51132547059283 84-85 22.183035348230636 27.428799846940173 27.84583419559641 22.54233060923279 86-87 22.07611419740959 27.397967688110388 27.96570397420281 22.560214140277207 88-89 22.15506441186298 27.29610272899521 27.92956760908622 22.619265250055587 90-91 22.178784720781582 27.334190802302967 27.929480905858206 22.557543571057252 92-93 22.14855409485794 27.23657712018858 27.98042885947534 22.63443992547814 94-95 22.101794248534407 27.26216559962335 27.87459175860666 22.761448393235586 96-97 22.058864444744906 27.19935061480241 27.929762528267833 22.812022412184856 98-99 22.01481599361884 27.141615485644703 27.904406551138266 22.939161969598192 100-101 22.186205474420937 26.926010027368445 28.145766554370226 22.74201794384039 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2949.0 1 1695.0 2 444.0 3 449.0 4 387.0 5 359.5 6 591.5 7 1135.5 8 1866.5 9 2306.5 10 2168.0 11 1960.0 12 2036.0 13 2178.5 14 2281.5 15 2401.0 16 2504.5 17 2689.0 18 2900.0 19 3036.0 20 3375.5 21 3947.5 22 4685.5 23 5689.0 24 6925.0 25 8717.0 26 11256.0 27 14332.0 28 16958.0 29 20214.0 30 24560.5 31 28204.5 32 31752.0 33 35497.5 34 39948.0 35 44735.5 36 50542.5 37 55879.5 38 58634.0 39 61020.0 40 63460.5 41 66783.5 42 69873.0 43 72630.5 44 75243.5 45 77415.0 46 79561.5 47 81513.0 48 81476.5 49 80271.5 50 78931.0 51 75102.0 52 71147.0 53 68767.5 54 66569.5 55 61805.0 56 54697.0 57 48198.5 58 42998.5 59 37600.5 60 31406.5 61 25433.5 62 19954.0 63 15217.0 64 11461.0 65 8559.5 66 6432.0 67 4905.5 68 3719.0 69 2707.0 70 1947.5 71 1442.0 72 1105.5 73 830.5 74 600.0 75 431.0 76 302.0 77 188.0 78 116.5 79 69.5 80 44.0 81 31.5 82 19.0 83 15.5 84 16.5 85 15.0 86 10.0 87 5.5 88 5.0 89 5.5 90 5.0 91 4.5 92 4.0 93 3.0 94 3.5 95 2.0 96 0.5 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.33495696855174956 2 0.29808052509717214 3 0.29954194293553055 4 0.29983422650320224 5 0.28570718739907086 6 0.2186768225463647 7 0.22861446384720197 8 0.22481477746747006 9 0.22233036714226073 10-11 0.23163472737980934 12-13 0.23122065899227445 14-15 0.21368364493197342 16-17 0.22690947636911712 18-19 0.2136105740400555 20-21 0.2169231211403346 22-23 0.21599755650937427 24-25 0.2066931962718257 26-27 0.2211368759076014 28-29 0.22174580000691738 30-31 0.22902853223473685 32-33 0.23019766650542356 34-35 0.21804354148307606 36-37 0.21543734633800354 38-39 0.20515870754154933 40-41 0.2050856366496314 42-43 0.204501069514288 44-45 0.2223547241062334 46-47 0.21171073085018954 48-49 0.22001645556485994 50-51 0.22905288919870948 52-53 0.23811367979653164 54-55 0.23392428199323756 56-57 0.1982656867372921 58-59 0.18528342493987485 60-61 0.19475828392523192 62-63 0.19534285106057528 64-65 0.19249308627577638 66-67 0.2066444823438804 68-69 0.1945147142855055 70-71 0.20053088438674765 72-73 0.2143169259952621 74-75 0.20367293273921824 76-77 0.21319650565252063 78-79 0.21083388014717452 80-81 0.19658505622317993 82-83 0.21049288265155755 84-85 0.19139702289700755 86-87 0.19731576514235913 88-89 0.20588941646072853 90-91 0.20148080598168064 92-93 0.20822768500210204 94-95 0.2059381303886738 96-97 0.19943482100797885 98-99 0.2080815432182662 100-101 0.1903983873741293 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2052801.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.703812725199334 #Duplication Level Percentage of deduplicated Percentage of total 1 80.44560318709435 49.63800433622169 2 11.421199565836385 14.094631182149927 3 3.462213698059218 6.408953569189975 4 1.4981532441863339 3.6976706885169355 5 0.8208264490814557 2.5324060747006256 6 0.4845325282248798 1.7938502628513209 7 0.3355324285502544 1.449254109414736 8 0.2404949559644033 1.1871564579346081 9 0.18418705657228146 1.0228539280627575 >10 1.0116984771104562 11.371165928775202 >50 0.06767308075751419 2.840246887043855 >100 0.02694075570594008 2.7817636231850216 >500 5.510008330743792E-4 0.22152680707809413 >1k 2.361432141747127E-4 0.38714978715618587 >5k 1.5742880944980846E-4 0.5733663577190784 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6599 0.3214632105109068 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5206 0.2536047088831309 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3384 0.1648479321668296 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3108 0.15140288805393218 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 4.871392794528062E-5 0.0 10-11 0.0 0.0 0.0 4.871392794528062E-5 0.0 12-13 0.0 0.0 0.0 4.871392794528062E-5 0.0 14-15 0.0 0.0 0.0 9.742785589056124E-5 0.0 16-17 0.0 0.0 0.0 9.742785589056124E-5 0.0 18-19 0.0 0.0 0.0 9.742785589056124E-5 0.0 20-21 0.0 4.871392794528062E-5 0.0 9.742785589056124E-5 0.0 22-23 0.0 4.871392794528062E-5 0.0 1.7049874780848218E-4 0.0 24-25 0.0 4.871392794528062E-5 0.0 3.653544595896046E-4 0.0 26-27 0.0 4.871392794528062E-5 0.0 6.576380272612884E-4 0.0 28-29 0.0 9.742785589056124E-5 0.0 0.0015832026582216201 0.0 30-31 0.0 9.742785589056124E-5 0.0 0.00392147119959509 0.0 32-33 0.0 9.742785589056124E-5 0.0 0.008451866498506187 0.0 34-35 0.0 9.742785589056124E-5 0.0 0.014638535347556825 0.0 36-37 0.0 9.742785589056124E-5 0.0 0.021336700440032913 0.0 38-39 0.0 9.742785589056124E-5 0.0 0.03044620496580039 0.0 40-41 0.0 9.742785589056124E-5 0.0 0.042259332492530936 0.0 42-43 0.0 9.742785589056124E-5 0.0 0.05653251338049815 0.0 44-45 0.0 9.742785589056124E-5 0.0 0.07436181100847086 0.0 46-47 0.0 9.742785589056124E-5 0.0 0.09484601770946136 0.0 48-49 0.0 9.742785589056124E-5 0.0 0.11861841454675831 2.435696397264031E-5 50-51 0.0 9.742785589056124E-5 0.0 0.14945433093612093 4.871392794528062E-5 52-53 0.0 1.2178481986320155E-4 0.0 0.19344300787070934 4.871392794528062E-5 54-55 0.0 1.4614178383584185E-4 0.0 0.2467116880788737 4.871392794528062E-5 56-57 0.0 1.4614178383584185E-4 0.0 0.29895737580018716 4.871392794528062E-5 58-59 0.0 1.4614178383584185E-4 0.0 0.3690080041855007 4.871392794528062E-5 60-61 0.0 1.4614178383584185E-4 0.0 0.4505307626019278 4.871392794528062E-5 62-63 0.0 1.4614178383584185E-4 0.0 0.5244054343309459 4.871392794528062E-5 64-65 0.0 1.4614178383584185E-4 0.0 0.6029322861787383 4.871392794528062E-5 66-67 0.0 1.4614178383584185E-4 0.0 0.7123681253078111 4.871392794528062E-5 68-69 0.0 1.4614178383584185E-4 0.0 0.8354682212255353 4.871392794528062E-5 70-71 0.0 1.4614178383584185E-4 0.0 0.9521867925824277 4.871392794528062E-5 72-73 0.0 1.9485571178112248E-4 0.0 1.0957467382371697 4.871392794528062E-5 74-75 0.0 1.9485571178112248E-4 0.0 1.260813883079753 4.871392794528062E-5 76-77 0.0 1.9485571178112248E-4 0.0 1.4395209277470151 4.871392794528062E-5 78-79 0.0 1.9485571178112248E-4 0.0 1.6407825210529419 4.871392794528062E-5 80-81 0.0 1.9485571178112248E-4 0.0 1.8680329949176757 4.871392794528062E-5 82-83 0.0 1.9485571178112248E-4 0.0 2.116815024934224 4.871392794528062E-5 84-85 0.0 1.9485571178112248E-4 0.0 2.3668392601133768 4.871392794528062E-5 86-87 0.0 1.9485571178112248E-4 0.0 2.6453855001044912 4.871392794528062E-5 88-89 0.0 1.9485571178112248E-4 0.0 2.9628785254878576 4.871392794528062E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3080 0.0 54.807495 1 GTATCAA 5885 0.0 43.228447 1 ATCAACG 7530 0.0 33.517437 3 TCAACGC 7550 0.0 33.304367 4 TATCAAC 7715 0.0 32.77772 2 CAACGCA 7780 0.0 32.382473 5 AACGCAG 8075 0.0 31.298834 6 TGGTATC 1410 0.0 29.329401 2 ACGCAGA 9715 0.0 25.91744 7 CGCAGAG 9680 0.0 25.912361 8 GTACATG 7320 0.0 24.490215 1 TACATGG 7235 0.0 23.651957 2 GCAGAGT 10450 0.0 23.501245 9 GTGGTAT 1905 0.0 23.463629 1 ACATGGG 7280 0.0 22.067675 3 GAGTACT 6155 0.0 21.223707 12-13 GTGTAGG 875 0.0 21.19429 1 AGAGTAC 9695 0.0 20.480715 10-11 AGTACTT 6435 0.0 20.189491 12-13 GTACTTT 6520 0.0 19.923365 14-15 >>END_MODULE