FastQCFastQC Report
Thu 26 May 2016
SRR937477_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937477_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2052801
Sequences flagged as poor quality0
Sequence length101
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73750.35926521859644456No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63780.3106974324349998No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40870.19909382351236188No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39100.054.3301351
GTATCAA69850.041.706531
ATCAACG89900.032.2337533
TATCAAC90450.032.0377462
TCAACGC91050.031.8788014
CAACGCA93500.031.0942825
AACGCAG97150.030.0194576
TGGTATC17350.027.6542972
ACGCAGA113300.025.6152787
CGCAGAG114050.025.3635398
GTGGTAT20900.024.7850741
GCAGAGT122900.022.6868429
CCCGTAA1050.002190452522.6303231
GTACATG74050.022.2697031
TACATGG72850.021.3887312
GAGTACT74700.021.26979612-13
TGCACCG3201.8189894E-1120.7835035
ACATGGG73350.020.4657523
GTACTTT77400.020.28185814-15
CAGAGTA118000.020.18723110-11
Produced by FastQC (version 0.11.5)