##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937477_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2052801 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.701386057391826 31.0 31.0 34.0 30.0 34.0 2 31.943989699927076 31.0 31.0 34.0 30.0 34.0 3 32.02341288804906 33.0 31.0 34.0 30.0 34.0 4 35.740099503069224 37.0 35.0 37.0 35.0 37.0 5 35.57729658159754 37.0 35.0 37.0 33.0 37.0 6 35.65805209564882 37.0 35.0 37.0 33.0 37.0 7 35.59735502856828 37.0 35.0 37.0 33.0 37.0 8 35.57061887635479 37.0 35.0 37.0 33.0 37.0 9 37.19127572521642 39.0 37.0 39.0 33.0 39.0 10-11 37.09199479150682 39.0 37.0 39.0 33.0 39.0 12-13 36.98975034599067 39.0 37.0 39.0 33.0 39.0 14-15 38.080145615673416 40.0 37.0 41.0 33.0 41.0 16-17 38.03458518385367 40.0 37.0 41.0 33.0 41.0 18-19 38.011600247661605 40.0 37.0 41.0 33.0 41.0 20-21 37.88345144025163 40.0 37.0 41.0 33.0 41.0 22-23 37.73356526034428 39.0 37.0 41.0 32.0 41.0 24-25 37.58029809026788 39.0 36.5 41.0 32.0 41.0 26-27 37.50544573000501 39.0 36.5 41.0 32.0 41.0 28-29 37.39684509117055 39.0 36.0 41.0 32.0 41.0 30-31 37.49868691607224 39.0 37.0 41.0 32.0 41.0 32-33 37.438116018065074 39.0 36.0 41.0 32.0 41.0 34-35 37.317971883295066 39.0 36.0 41.0 31.5 41.0 36-37 37.14256106656222 39.0 36.0 41.0 31.0 41.0 38-39 37.00568272326446 39.0 36.0 41.0 31.0 41.0 40-41 36.82530406015975 39.0 35.0 40.5 31.0 41.0 42-43 36.608706104488455 39.0 35.0 40.0 30.0 41.0 44-45 36.330311608382885 39.0 35.0 40.0 30.0 41.0 46-47 36.01352493495473 38.5 35.0 40.0 29.5 41.0 48-49 35.73148566276029 38.0 35.0 40.0 28.5 41.0 50-51 35.50183481009606 38.0 35.0 40.0 28.0 41.0 52-53 35.24645447853932 38.0 34.0 40.0 27.0 41.0 54-55 34.9820006907635 38.0 34.0 40.0 26.5 41.0 56-57 34.69712870365905 38.0 34.0 40.0 26.0 41.0 58-59 34.42553126191969 38.0 33.0 40.0 26.0 41.0 60-61 34.062063005620125 37.0 33.0 40.0 25.0 41.0 62-63 33.64039987314894 36.5 32.5 39.0 23.5 40.5 64-65 33.244966024470955 36.0 32.0 39.0 23.0 40.0 66-67 32.76760460463532 35.5 31.0 39.0 22.0 40.0 68-69 32.33155430068477 35.0 31.0 38.0 21.5 40.0 70-71 31.905107460489354 35.0 31.0 38.0 20.0 40.0 72-73 31.406742787050476 34.5 30.0 37.0 18.5 39.0 74-75 30.91315768065195 34.0 30.0 36.5 18.0 39.0 76-77 29.676515648618643 33.0 28.5 35.0 17.0 37.5 78-79 30.354172664569045 34.0 29.5 36.0 17.5 38.0 80-81 30.210340895196367 34.0 30.0 35.0 15.5 37.0 82-83 29.833144566862543 34.0 29.0 35.0 11.0 37.0 84-85 29.39537417411624 34.0 29.0 35.0 7.0 36.0 86-87 28.92708767191754 33.5 29.0 35.0 4.5 36.0 88-89 28.43167287038539 33.0 28.5 35.0 2.0 35.5 90-91 27.855072410818195 33.0 27.0 35.0 2.0 35.0 92-93 27.335843074901074 32.5 26.0 34.0 2.0 35.0 94-95 26.754162726927746 32.0 25.0 34.0 2.0 35.0 96-97 26.072879202611453 32.0 24.5 34.0 2.0 35.0 98-99 25.314636927787937 32.0 22.0 34.0 2.0 35.0 100-101 23.186150045718023 30.0 9.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.04364996570645374 1101 2 0.1431242383611817 1101 3 -0.053840930044835034 1101 4 0.06752980445123313 1101 5 0.0633162968099299 1101 6 0.06483101602390917 1101 7 0.00394691338171782 1101 8 0.05982890603235802 1101 9 0.08742628527001983 1101 10-11 -0.006305102768926929 1101 12-13 0.09333902177871067 1101 14-15 0.09320438104860074 1101 16-17 0.0752100571053873 1101 18-19 0.014330029190503524 1101 20-21 0.04010689138950596 1101 22-23 0.023311471369055425 1101 24-25 7.348649928289319E-4 1101 26-27 -0.025129983645456377 1101 28-29 -0.0831533988362807 1101 30-31 -0.01176225363112593 1101 32-33 -0.017447472956988008 1101 34-35 -0.18512690414636523 1101 36-37 -0.19288463719326643 1101 38-39 -0.20776176539304458 1101 40-41 -0.258111110922151 1101 42-43 -0.0759057678687256 1101 44-45 -0.09163597745116903 1101 46-47 -0.1101498749791574 1101 48-49 -0.025992918652320895 1101 50-51 -0.1606783451082947 1101 52-53 -0.1808622283411978 1101 54-55 -0.12492641625605216 1101 56-57 -0.1834126803611582 1101 58-59 -0.30664998900241613 1101 60-61 -0.24212655098020264 1101 62-63 -0.2951123077584157 1101 64-65 -0.19049774647224638 1101 66-67 -0.23704202737621927 1101 68-69 -0.11230440759271687 1101 70-71 -0.5356477189735394 1101 72-73 -0.7137659132361165 1101 74-75 -0.40560200171272953 1101 76-77 -0.30440275626610713 1101 78-79 -0.3429570612663646 1101 80-81 -0.3695560844142243 1101 82-83 -0.2564357129853114 1101 84-85 -0.5082913896127579 1101 86-87 -0.5668896444785361 1101 88-89 -0.39980765092207093 1101 90-91 -0.2876799290396299 1101 92-93 -0.27728803671240954 1101 94-95 -0.3368355651628079 1101 96-97 -0.3122582053576224 1101 98-99 -0.1385699270335934 1101 100-101 0.32868960647605405 1102 1 0.08349894916024425 1102 2 0.08327625863572052 1102 3 0.14822256487907026 1102 4 0.08879121193024275 1102 5 0.03239274467350839 1102 6 0.0679983795841892 1102 7 0.03328949651726276 1102 8 0.05599652401257771 1102 9 0.053428766687964924 1102 10-11 0.07957790397485098 1102 12-13 0.14037309176725188 1102 14-15 0.09260824780540844 1102 16-17 0.0772308838868696 1102 18-19 0.06093325170099462 1102 20-21 0.035349354755837226 1102 22-23 0.05799871407889867 1102 24-25 0.09047893170859567 1102 26-27 0.134697088302687 1102 28-29 -0.004912194393291713 1102 30-31 0.11482319629978122 1102 32-33 0.06676940289402467 1102 34-35 0.12398121347742119 1102 36-37 0.0825087690867008 1102 38-39 -0.06604530469414271 1102 40-41 0.07500287483072299 1102 42-43 0.06255690787335766 1102 44-45 0.1577040905504603 1102 46-47 0.13326393295103145 1102 48-49 0.18303621834090222 1102 50-51 0.17170658121746385 1102 52-53 0.09942348157085235 1102 54-55 0.21618794308527356 1102 56-57 0.2156844291919171 1102 58-59 0.1516314382062589 1102 60-61 0.13465504257997196 1102 62-63 -0.03848910562865626 1102 64-65 -0.06151729999140798 1102 66-67 0.17077860520902988 1102 68-69 0.1455840154086303 1102 70-71 0.17838727084094685 1102 72-73 0.14562279296000824 1102 74-75 0.191060911630057 1102 76-77 0.25116775187039053 1102 78-79 0.2563261061902047 1102 80-81 0.33474806929650214 1102 82-83 0.4075738832775677 1102 84-85 0.15892190207553725 1102 86-87 0.3030161193829457 1102 88-89 0.354985975364297 1102 90-91 0.19672575624813504 1102 92-93 0.19496061896014183 1102 94-95 0.3336133939922341 1102 96-97 0.0842282416940634 1102 98-99 0.0939414148058546 1102 100-101 0.5392643494547507 1103 1 0.11904294569253793 1103 2 0.12618913249787767 1103 3 0.1789984643155691 1103 4 0.13213754916475295 1103 5 0.008552259194537726 1103 6 0.002111946987746194 1103 7 0.06840002967721404 1103 8 0.03865798911877505 1103 9 0.11411363881627068 1103 10-11 0.0821786842089196 1103 12-13 0.2192634255340522 1103 14-15 0.14072268213570283 1103 16-17 0.1968667746541044 1103 18-19 0.18967187328746604 1103 20-21 0.14241480772506065 1103 22-23 0.10806373358475696 1103 24-25 0.09438010205970215 1103 26-27 0.1507352330794518 1103 28-29 -0.04002272755323588 1103 30-31 0.020978376187088088 1103 32-33 0.09212701017621328 1103 34-35 0.15519057628626598 1103 36-37 0.052816528081059744 1103 38-39 0.1673747213136565 1103 40-41 0.21631193421520578 1103 42-43 0.23464186669433929 1103 44-45 0.05193902769827474 1103 46-47 0.12112695852537314 1103 48-49 0.3867640033430675 1103 50-51 0.19424667657940375 1103 52-53 0.44294320415428956 1103 54-55 0.132962975595035 1103 56-57 0.01802513140257389 1103 58-59 0.14382909750404593 1103 60-61 0.037559247174684174 1103 62-63 0.08743200403758067 1103 64-65 0.02084074075414577 1103 66-67 0.2696082541036944 1103 68-69 0.3235207297562681 1103 70-71 0.19594253742091539 1103 72-73 0.3487003829036581 1103 74-75 0.4056252809408498 1103 76-77 0.27349111554615746 1103 78-79 0.3072580524407442 1103 80-81 0.31892665620590677 1103 82-83 0.18477370989221598 1103 84-85 0.27053872045438254 1103 86-87 0.3793056729156703 1103 88-89 0.3317956849438346 1103 90-91 0.42234344155372483 1103 92-93 0.5534348278894861 1103 94-95 0.5952085392024635 1103 96-97 0.3356369976541842 1103 98-99 0.33754783006376243 1103 100-101 0.646979997482493 1104 1 0.05889091454512396 1104 2 0.07666073364077874 1104 3 0.059364970410882734 1104 4 0.05429353808534643 1104 5 -0.017590504944784868 1104 6 -0.017923980138043305 1104 7 -0.08490864547176358 1104 8 0.07640599294712302 1104 9 0.10926939524119916 1104 10-11 0.05053429084838257 1104 12-13 -0.04096937988108351 1104 14-15 -0.07899457289618539 1104 16-17 -0.03280796925496787 1104 18-19 -0.042845918346628764 1104 20-21 -0.18017154708397243 1104 22-23 -0.10746767757544262 1104 24-25 -0.06217143592402152 1104 26-27 -0.048395461750715185 1104 28-29 -0.030839609651756916 1104 30-31 -0.052305972650813715 1104 32-33 -0.090985360086691 1104 34-35 0.07038635294070872 1104 36-37 -0.040852583569765955 1104 38-39 -0.11125483380836698 1104 40-41 0.058206030871822634 1104 42-43 0.32222515997241175 1104 44-45 0.1923643823002621 1104 46-47 0.015829007308163057 1104 48-49 0.12243173329483881 1104 50-51 -0.1896351971844794 1104 52-53 0.10534170393958675 1104 54-55 0.041898608220165556 1104 56-57 -0.0065047286188004705 1104 58-59 0.14034581091875253 1104 60-61 0.04410305066735276 1104 62-63 -0.03664849956905414 1104 64-65 -0.08420498677383392 1104 66-67 0.041611498349332976 1104 68-69 0.0667630984685843 1104 70-71 0.06427799197760464 1104 72-73 -0.06704104872758876 1104 74-75 -0.06016144217377217 1104 76-77 0.1144034204300226 1104 78-79 -0.03319055172791252 1104 80-81 -0.14586656738431003 1104 82-83 0.029225074509032112 1104 84-85 0.04464511939519156 1104 86-87 -0.0942915033590026 1104 88-89 0.03091006267130325 1104 90-91 0.10133827852743238 1104 92-93 -0.11360418215465629 1104 94-95 -0.24112714102804844 1104 96-97 -0.009541169435529184 1104 98-99 0.1912290514016668 1104 100-101 0.48898453419016974 1105 1 -0.031151836028481483 1105 2 0.04686588895180677 1105 3 0.0670857504418052 1105 4 0.09242555042251155 1105 5 -0.02861431862044128 1105 6 -0.01610071136062885 1105 7 -0.01082135274432261 1105 8 -0.056282668472285025 1105 9 0.007353215627588838 1105 10-11 -0.02128536905559031 1105 12-13 -0.06655363077909726 1105 14-15 0.053187162220474704 1105 16-17 0.04105053212276033 1105 18-19 0.02034187037003221 1105 20-21 0.08329079125234529 1105 22-23 0.08335299980267052 1105 24-25 -0.041085782490675626 1105 26-27 -0.018636289318095578 1105 28-29 -0.027979310356087694 1105 30-31 0.0021382200959862985 1105 32-33 -0.06280237625998808 1105 34-35 -0.1629983882788011 1105 36-37 -0.10951633525174742 1105 38-39 0.048944833045347025 1105 40-41 -0.006816796656082147 1105 42-43 -0.20272848872902216 1105 44-45 -0.0700676253832242 1105 46-47 -0.2131771002529277 1105 48-49 -0.10906301210937386 1105 50-51 -0.12806904751202097 1105 52-53 0.061577368264742915 1105 54-55 -0.10856995682620152 1105 56-57 -0.07466544716307766 1105 58-59 -0.17931563220690805 1105 60-61 -0.2237884419340972 1105 62-63 -0.09424063313032605 1105 64-65 -0.22477078859652266 1105 66-67 -0.3338289498970113 1105 68-69 -0.4795169461271698 1105 70-71 -0.2094893731228673 1105 72-73 -0.3168840093148191 1105 74-75 -0.36767005376428585 1105 76-77 -0.3272542421607696 1105 78-79 -0.22896215802426667 1105 80-81 -0.325908791022556 1105 82-83 -0.3976906207224218 1105 84-85 -0.32663208681163525 1105 86-87 -0.0763421112450331 1105 88-89 0.06919798268722488 1105 90-91 -0.07897909228603694 1105 92-93 -0.22089627944611934 1105 94-95 -0.27729482525940696 1105 96-97 -0.41412675650398967 1105 98-99 -0.11100676611500404 1105 100-101 0.17686904082384913 1106 1 -0.1463901961025016 1106 2 0.037782203629639355 1106 3 -0.026250438420806432 1106 4 0.011440833858088695 1106 5 -0.07056032444886284 1106 6 -0.29807133304748135 1106 7 -0.21720304954916259 1106 8 -0.03642403212207057 1106 9 -0.25514691986055027 1106 10-11 -0.2735262493958288 1106 12-13 -0.29735888629851104 1106 14-15 -0.2072589731159198 1106 16-17 -0.07395497523639705 1106 18-19 -0.13706179943360297 1106 20-21 -0.08266685197916956 1106 22-23 -0.06436906151570554 1106 24-25 0.019304550421423983 1106 26-27 0.05784106398002109 1106 28-29 0.09349654976931987 1106 30-31 0.026475159843194263 1106 32-33 -0.05032831055814313 1106 34-35 -0.07889267912641884 1106 36-37 -0.002607225178941519 1106 38-39 0.09837165281921756 1106 40-41 0.042530131837203555 1106 42-43 -0.34557045424514143 1106 44-45 -0.2627614696619389 1106 46-47 -0.07334577457346114 1106 48-49 -0.05700734872394975 1106 50-51 -0.2332746512266013 1106 52-53 -0.06873138396987599 1106 54-55 -0.1941413672969503 1106 56-57 -0.09484878784079598 1106 58-59 -0.10912418092900822 1106 60-61 -0.09697014442009078 1106 62-63 -0.02111432193220253 1106 64-65 -0.012278296508092978 1106 66-67 -0.17390688267321508 1106 68-69 0.09936555519687573 1106 70-71 0.2708612963380297 1106 72-73 0.458459093367253 1106 74-75 0.11397064963779258 1106 76-77 0.03469951172026242 1106 78-79 0.10183408987006004 1106 80-81 0.13045029327946978 1106 82-83 -0.020977443314439626 1106 84-85 -0.12425336364436035 1106 86-87 0.2119876694959153 1106 88-89 0.19190603733547462 1106 90-91 -0.03645403819356474 1106 92-93 -0.14403152351725268 1106 94-95 -0.7097237145444844 1106 96-97 -0.1312757422491444 1106 98-99 0.07237670717290001 1106 100-101 0.3004137580759938 1107 1 -0.03621432622587761 1107 2 -0.009294179162282035 1107 3 0.015729876721749747 1107 4 0.03666610071267229 1107 5 -0.064437132359771 1107 6 0.006752103947114563 1107 7 -0.06317079595644515 1107 8 -0.07212959101491379 1107 9 0.053190772482686555 1107 10-11 -0.06232778352086399 1107 12-13 -0.011845647538748949 1107 14-15 -0.06991615161968667 1107 16-17 -0.1590915216067046 1107 18-19 -0.0879563976499469 1107 20-21 0.07962786637950359 1107 22-23 -0.04045852133734229 1107 24-25 -0.061481919704725385 1107 26-27 -0.08643822071107365 1107 28-29 -0.13720850082907532 1107 30-31 -0.1054314063968178 1107 32-33 -0.1353735487633969 1107 34-35 0.020975441570406872 1107 36-37 -0.1816972857121968 1107 38-39 -0.12839513244949075 1107 40-41 -0.15995672182020115 1107 42-43 -0.10687233493457171 1107 44-45 -0.08913733029684323 1107 46-47 -0.10133976745733264 1107 48-49 -0.23930557893512372 1107 50-51 -0.15528851380226882 1107 52-53 -0.07376183542776005 1107 54-55 -0.2000349027158279 1107 56-57 -0.21121448258688247 1107 58-59 -0.1442366064176568 1107 60-61 -0.04860204682755409 1107 62-63 -0.10616131606371226 1107 64-65 0.03422399081689065 1107 66-67 -0.05147677315886767 1107 68-69 -0.08921926066191332 1107 70-71 -0.21068853962148992 1107 72-73 -0.10827331271379848 1107 74-75 -0.05508660279971167 1107 76-77 0.028064975423419014 1107 78-79 -0.07280737771461787 1107 80-81 0.07751456942197876 1107 82-83 0.1502354396392107 1107 84-85 0.11603959878805625 1107 86-87 0.23309482720581798 1107 88-89 0.11437958292024675 1107 90-91 0.283774509470593 1107 92-93 0.35706934982501437 1107 94-95 0.31487062028292456 1107 96-97 0.4413887372111418 1107 98-99 0.613933566837428 1107 100-101 0.8609472824048119 1108 1 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78-79 -0.48466780548306687 2316 80-81 -0.46609459913047147 2316 82-83 -0.45808283176745945 2316 84-85 -0.6121901990074008 2316 86-87 -0.7133005301195325 2316 88-89 -0.8505266146124235 2316 90-91 -0.6502370242179971 2316 92-93 -0.6222675360790682 2316 94-95 -0.42931099881335655 2316 96-97 -0.487430738663015 2316 98-99 -0.7662290727713028 2316 100-101 -0.9268608787748356 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 6.0 4 22.0 5 56.0 6 124.0 7 337.0 8 772.0 9 1403.0 10 2109.0 11 3516.0 12 4965.0 13 6744.0 14 8797.0 15 11559.0 16 14918.0 17 18478.0 18 16938.0 19 12890.0 20 12344.0 21 12725.0 22 14080.0 23 15866.0 24 18999.0 25 22922.0 26 28339.0 27 34908.0 28 42616.0 29 53098.0 30 64644.0 31 79502.0 32 99344.0 33 124659.0 34 162163.0 35 218954.0 36 305743.0 37 391423.0 38 233120.0 39 13709.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.148104752788335 20.69092741660347 15.582932539999728 22.578035290608472 2 16.179015890970437 21.49886910616275 38.80853526474315 23.513579738123667 3 19.630836111245074 24.844541677444624 30.090203580376272 25.434418630934026 4 13.802360774376085 17.802992106882254 36.594535953558086 31.80011116518357 5 13.70908881844678 37.50961054046074 34.342151806099494 14.439148834992988 6 31.746477130515814 37.09102830717639 17.08626408502334 14.07623047728445 7 28.20112616858624 32.748425200494346 20.784966492124664 18.26548213879475 8 26.206290819226997 35.98687841636866 19.475779678595245 18.331051085809097 9 25.78301549931045 17.01031907135665 20.242390762670126 36.964274666662774 10-11 24.746967679770226 27.32632145054489 27.236590395269683 20.690120474415203 12-13 25.47281189996519 24.287082836133365 28.080904330322486 22.15920093357896 14-15 22.509074186928007 25.898784149072412 26.16929259095256 25.422849073047022 16-17 21.95680438581236 29.16334803032539 26.824105210393018 22.055742373469226 18-19 21.83808854340971 27.698374075226972 29.20002961806819 21.263507763295127 20-21 22.501815811664162 26.701175613223104 29.54716506860626 21.249843506506476 22-23 22.28315051192835 26.565129440477598 29.394077049266176 21.757642998327874 24-25 22.117432946365373 26.781678850855684 29.440392790909183 21.660495411869757 26-27 21.942773465044695 27.008778132393207 29.32515869399005 21.723289708572043 28-29 21.932878052677744 27.151571336951125 29.332061528898272 21.583489081472862 30-31 22.114199087003563 26.91220434908206 29.38090443252902 21.59269213138536 32-33 21.87403942223333 26.893985340030525 29.30788225453904 21.924092983197106 34-35 22.1731916537453 26.922117633418924 29.285254635008464 21.61943607782732 36-37 22.261266435470365 26.84173965230921 29.208091773143135 21.68890213907729 38-39 22.071209045591853 27.03364817144964 29.16395695442471 21.731185828533796 40-41 22.301504139953167 27.022078613562638 29.028386092952996 21.6480311535312 42-43 22.081537866018937 26.949366542809972 28.965097065466054 22.003998525705036 44-45 22.181180737928326 27.141987947199947 28.934222070234767 21.742609244636963 46-47 22.18181401899161 27.332094051006408 28.47567786648584 22.010414063516144 48-49 22.314705614426337 27.314654464801997 28.35623131516401 22.014408605607656 50-51 22.42102376216691 27.492703871441996 28.225239562919153 21.86103280347194 52-53 22.49024625377716 27.557566466501136 27.943064135296115 22.00912314442559 54-55 22.38714322528097 27.471026173506345 28.074518669856452 22.06731193135623 56-57 22.333850188108833 27.74976726920924 27.986906670446864 21.929475872235056 58-59 22.41430124011046 27.475239928273616 28.14510515144917 21.965353680166757 60-61 22.385852306190422 27.488465759710756 28.269715379133192 21.85596655496563 62-63 22.23077151657662 27.52448970942629 28.38119720323597 21.86354157076112 64-65 22.202888200777426 27.38480919115131 28.303968164466053 22.108334443605212 66-67 22.193407933842586 27.544145779352213 28.095733585476623 22.166712701328574 68-69 22.11288381094904 27.529117532581093 28.183784010237716 22.174214646232148 70-71 22.25549383500885 27.404239378293365 28.147102422494925 22.193164364202865 72-73 22.202993719546132 27.44985805955044 28.068331143232587 22.278817077670837 74-75 22.11851026962672 27.71001670400589 27.75982669532994 22.411646331037446 76-77 22.195673131492043 27.441870887631097 27.99745323584702 22.36500274502984 78-79 22.26491998006626 27.431908889366284 27.93288292435555 22.370288206211903 80-81 22.127400561476733 27.704779956751775 27.819769183666608 22.348050298104884 82-83 22.249713195910942 27.557500870764006 27.80119300758235 22.391592925742707 84-85 22.239932657045873 27.55130126229067 27.759239196093482 22.449526884569966 86-87 22.07690857516145 27.497063767993097 27.924991268028414 22.501036388817035 88-89 22.17555427925064 27.42270195698463 27.846147775648976 22.55559598811575 90-91 22.23679457233008 27.505835909545656 27.749308374989983 22.508061143134285 92-93 22.062839600113406 27.414528186626736 27.854126741173683 22.668505472086174 94-95 22.0449035245014 27.40652893290679 27.79726334895589 22.751304193635917 96-97 21.955830107253455 27.414737229765574 27.75125304401157 22.878179618969398 98-99 21.92594645796945 27.32377288857138 27.732799882696717 23.01748077076245 100-101 21.96518668503733 27.30230912992047 27.89993218185397 22.832572003188233 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 399.0 1 281.0 2 222.5 3 314.0 4 286.5 5 270.0 6 504.5 7 1082.0 8 1781.5 9 2208.0 10 2100.5 11 1878.0 12 1947.5 13 2157.0 14 2371.0 15 2548.0 16 2684.5 17 2859.0 18 3060.5 19 3318.0 20 3662.0 21 4167.5 22 4839.5 23 5849.5 24 7194.5 25 8951.5 26 11608.5 27 14821.5 28 17461.5 29 20620.0 30 24923.0 31 28485.5 32 31870.0 33 35911.0 34 40715.5 35 45327.0 36 50970.5 37 56470.5 38 59610.0 39 62122.5 40 64081.0 41 67265.5 42 70522.0 43 73096.5 44 76245.0 45 78522.0 46 80184.5 47 81945.0 48 81866.5 49 81028.5 50 79319.5 51 74854.0 52 70840.5 53 68522.0 54 66527.5 55 61491.5 56 53712.0 57 47062.0 58 41767.0 59 36417.5 60 30455.5 61 24510.0 62 19091.0 63 14477.5 64 10844.0 65 7952.0 66 5824.5 67 4521.0 68 3416.0 69 2434.0 70 1791.0 71 1279.5 72 944.0 73 765.5 74 567.0 75 387.5 76 248.5 77 140.0 78 87.5 79 53.0 80 30.0 81 20.0 82 14.0 83 10.0 84 5.5 85 2.0 86 0.5 87 0.5 88 0.5 89 1.0 90 2.0 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.050516343279256005 2 0.0 3 0.0 4 0.0 5 0.017878011555917988 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0027036230009630743 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0011447773067140947 24-25 1.9485571178112248E-4 26-27 0.0014370608743857782 28-29 0.003629187631923406 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.014346251779885142 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 2.435696397264031E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 1.7049874780848218E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.922835676716837E-4 84-85 0.0012178481986320155 86-87 0.0 88-89 0.0 90-91 0.010108140048645728 92-93 9.255646309603318E-4 94-95 0.0 96-97 0.0 98-99 1.2178481986320155E-4 100-101 0.012324623770155996 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2052801.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.693995492028044 #Duplication Level Percentage of deduplicated Percentage of total 1 79.5828339999369 48.30200168034985 2 11.955466379096162 14.512500450359106 3 3.625023808716482 6.600515361141975 4 1.566138241173775 3.8022074939877526 5 0.8131396784361508 2.467634798869643 6 0.4991187522489126 1.8176106779389296 7 0.3351236910605852 1.423799705615207 8 0.26611778305868833 1.292140122025002 9 0.1924292574332429 1.0511370434868792 >10 1.0670837086440172 11.814193466090497 >50 0.06938821121311478 2.8771883375810003 >100 0.027335416753259383 2.876122522960896 >500 4.0053611438665163E-4 0.1510145717955133 >1k 2.4032166863197912E-4 0.3432591361481904 >5k 1.6021444575465275E-4 0.6686746316495841 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7375 0.35926521859644456 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6378 0.3106974324349998 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4087 0.19909382351236188 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.4614178383584185E-4 0.0 14-15 0.0 0.0 0.0 1.9485571178112248E-4 0.0 16-17 0.0 4.871392794528062E-5 0.0 1.9485571178112248E-4 0.0 18-19 0.0 4.871392794528062E-5 0.0 1.9485571178112248E-4 0.0 20-21 0.0 4.871392794528062E-5 0.0 1.9485571178112248E-4 0.0 22-23 0.0 4.871392794528062E-5 0.0 3.1664053164432403E-4 0.0 24-25 0.0 4.871392794528062E-5 0.0 4.627823154801659E-4 0.0 26-27 0.0 4.871392794528062E-5 0.0 7.06351955206569E-4 0.0 28-29 0.0 4.871392794528062E-5 0.0 0.0016075596221942605 0.0 30-31 0.0 4.871392794528062E-5 0.0 0.003994542091513011 0.0 32-33 0.0 4.871392794528062E-5 0.0 0.008451866498506187 0.0 34-35 0.0 4.871392794528062E-5 0.0 0.014735963203447387 0.0 36-37 0.0 4.871392794528062E-5 0.0 0.021555913115786675 0.0 38-39 0.0 4.871392794528062E-5 0.0 0.03086027335333527 0.0 40-41 0.0 4.871392794528062E-5 0.0 0.042649043916093185 0.0 42-43 0.0 4.871392794528062E-5 0.0 0.05694658176803305 0.0 44-45 0.0 4.871392794528062E-5 0.0 0.07501944903573216 0.0 46-47 0.0 4.871392794528062E-5 0.0 0.09562544055658585 0.0 48-49 0.0 4.871392794528062E-5 0.0 0.11920298168210167 0.0 50-51 0.0 4.871392794528062E-5 0.0 0.15001454110749168 0.0 52-53 0.0 4.871392794528062E-5 0.0 0.19368657751043575 0.0 54-55 4.871392794528062E-5 4.871392794528062E-5 0.0 0.24690654379065483 0.0 56-57 4.871392794528062E-5 4.871392794528062E-5 0.0 0.2991765884759409 0.0 58-59 4.871392794528062E-5 4.871392794528062E-5 0.0 0.368715720617829 0.0 60-61 4.871392794528062E-5 4.871392794528062E-5 0.0 0.4496295549349401 0.0 62-63 4.871392794528062E-5 4.871392794528062E-5 0.0 0.5229927304205327 0.0 64-65 4.871392794528062E-5 4.871392794528062E-5 0.0 0.6012272987006534 0.0 66-67 4.871392794528062E-5 4.871392794528062E-5 0.0 0.710005499802465 0.0 68-69 4.871392794528062E-5 4.871392794528062E-5 0.0 0.8325210285848458 0.0 70-71 4.871392794528062E-5 4.871392794528062E-5 0.0 0.9486550328063947 0.0 72-73 4.871392794528062E-5 4.871392794528062E-5 0.0 1.092458548100863 0.0 74-75 4.871392794528062E-5 4.871392794528062E-5 0.0 1.2584025436464614 0.0 76-77 4.871392794528062E-5 4.871392794528062E-5 0.0 1.4377428693770122 0.0 78-79 4.871392794528062E-5 4.871392794528062E-5 0.0 1.6395159589263644 0.0 80-81 4.871392794528062E-5 4.871392794528062E-5 0.0 1.8679842809897305 0.0 82-83 4.871392794528062E-5 4.871392794528062E-5 0.0 2.1175944477813484 0.0 84-85 4.871392794528062E-5 4.871392794528062E-5 0.0 2.3679109665281732 0.0 86-87 4.871392794528062E-5 4.871392794528062E-5 0.0 2.6457752115280533 0.0 88-89 4.871392794528062E-5 4.871392794528062E-5 0.0 2.962367029244432 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3910 0.0 54.330135 1 GTATCAA 6985 0.0 41.70653 1 ATCAACG 8990 0.0 32.233753 3 TATCAAC 9045 0.0 32.037746 2 TCAACGC 9105 0.0 31.878801 4 CAACGCA 9350 0.0 31.094282 5 AACGCAG 9715 0.0 30.019457 6 TGGTATC 1735 0.0 27.654297 2 ACGCAGA 11330 0.0 25.615278 7 CGCAGAG 11405 0.0 25.363539 8 GTGGTAT 2090 0.0 24.785074 1 GCAGAGT 12290 0.0 22.686842 9 CCCGTAA 105 0.0021904525 22.630323 1 GTACATG 7405 0.0 22.269703 1 TACATGG 7285 0.0 21.388731 2 GAGTACT 7470 0.0 21.269796 12-13 TGCACCG 320 1.8189894E-11 20.783503 5 ACATGGG 7335 0.0 20.465752 3 GTACTTT 7740 0.0 20.281858 14-15 CAGAGTA 11800 0.0 20.187231 10-11 >>END_MODULE