FastQCFastQC Report
Thu 26 May 2016
SRR937476_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937476_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2133799
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68920.322991996903176No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52820.24753971672120942No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34820.16318313018236488No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN31710.1486081866192645No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35300.054.5164451
GTATCAA65950.043.5900961
ATCAACG80950.034.919313
TCAACGC82650.034.2042854
CAACGCA83850.033.7722365
TATCAAC84500.033.3952832
AACGCAG87450.032.4890946
ACGCAGA103050.027.293537
CGCAGAG103450.026.9124438
TGGTATC15750.025.9401952
TAGGCGA950.001225042325.0064934
GCAGAGT113250.024.3738389
GTACATG75450.024.3724371
TACATGG73850.024.1232682
ACATGGG75500.022.652763
GAGTACT68600.021.33106812-13
GTGGTAT20250.021.118581
GTACTTT69500.020.71206514-15
CAGAGTA109550.020.46965210-11
AGAGTAC105200.020.43542910-11
Produced by FastQC (version 0.11.5)