##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937476_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2133799 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.643480009129256 31.0 31.0 34.0 30.0 34.0 2 31.7400158121735 33.0 31.0 34.0 30.0 34.0 3 31.792369384370318 33.0 31.0 34.0 30.0 34.0 4 35.42186213415603 37.0 35.0 37.0 33.0 37.0 5 35.43240295829176 37.0 35.0 37.0 33.0 37.0 6 35.45292082337652 37.0 35.0 37.0 33.0 37.0 7 35.34204861845 37.0 35.0 37.0 33.0 37.0 8 35.34449823999355 37.0 35.0 37.0 33.0 37.0 9 36.95745428693143 39.0 37.0 39.0 33.0 39.0 10-11 36.84155724133342 39.0 37.0 39.0 33.0 39.0 12-13 36.68363186973094 39.0 37.0 39.0 32.5 39.0 14-15 37.83574062036771 40.0 38.0 41.0 33.0 41.0 16-17 37.746480807236296 40.0 37.5 41.0 33.0 41.0 18-19 37.685304942030626 40.0 37.0 41.0 32.0 41.0 20-21 37.56058349450909 40.0 37.0 41.0 32.0 41.0 22-23 37.398873324057234 40.0 37.0 41.0 32.0 41.0 24-25 37.33468147655894 39.5 37.0 41.0 32.0 41.0 26-27 37.37108579580364 40.0 37.0 41.0 32.0 41.0 28-29 37.28814686856634 40.0 37.0 41.0 31.5 41.0 30-31 37.08254573181448 39.0 36.5 41.0 31.0 41.0 32-33 36.85636627442416 39.0 36.0 41.0 30.5 41.0 34-35 36.88099699175039 39.0 36.0 41.0 30.5 41.0 36-37 36.74999332177023 39.0 36.0 41.0 30.0 41.0 38-39 36.639189773732205 39.0 36.0 41.0 30.0 41.0 40-41 36.420997947791705 39.0 35.5 41.0 30.0 41.0 42-43 36.15769948340964 39.0 35.0 41.0 30.0 41.0 44-45 35.94411657330423 39.0 35.0 40.0 29.0 41.0 46-47 35.63830918469828 39.0 35.0 40.0 27.5 41.0 48-49 35.34100587731085 39.0 35.0 40.0 26.5 41.0 50-51 34.38669152998947 37.5 34.0 39.5 25.0 40.5 52-53 34.52983762763034 38.0 34.0 39.5 25.5 40.5 54-55 34.8972860142872 38.0 34.0 40.0 26.0 41.0 56-57 34.667535227076215 38.0 34.0 40.0 24.5 41.0 58-59 34.38674729906613 38.0 34.0 40.0 24.0 41.0 60-61 34.06281800675696 38.0 33.5 40.0 23.0 41.0 62-63 33.73861197797918 37.0 33.0 40.0 22.5 41.0 64-65 33.30046363317257 37.0 33.0 39.5 21.5 41.0 66-67 32.93222721540314 36.0 32.0 39.0 20.5 41.0 68-69 32.4262639077064 36.0 31.5 39.0 18.5 40.0 70-71 31.93953343309281 35.0 31.0 38.5 17.0 40.0 72-73 31.454746674827383 35.0 31.0 37.5 12.5 40.0 74-75 30.939530386882737 35.0 30.0 37.0 8.0 39.0 76-77 30.42900104461573 34.5 30.0 36.5 7.0 39.0 78-79 29.90344263916142 34.0 29.0 36.0 2.0 38.5 80-81 29.38188578211912 34.0 29.0 35.5 2.0 37.0 82-83 28.880115465421063 34.0 29.0 35.0 2.0 37.0 84-85 28.310691634966552 34.0 27.5 35.0 2.0 36.0 86-87 27.80522579680654 33.0 26.5 35.0 2.0 36.0 88-89 27.3043346631993 33.0 26.0 35.0 2.0 35.5 90-91 26.745969043944626 32.5 25.0 35.0 2.0 35.0 92-93 26.16656442335946 32.0 24.5 34.5 2.0 35.0 94-95 25.54638768693771 32.0 21.5 34.0 2.0 35.0 96-97 24.727243990647665 31.5 18.0 34.0 2.0 35.0 98-99 23.70849128713623 31.0 2.0 34.0 2.0 35.0 100-101 21.09522312082816 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.0314606791215013 1101 2 0.06595798336024927 1101 3 -0.0511156640450281 1101 4 -0.013724649912376208 1101 5 0.04760823577024098 1101 6 -0.12390960747486446 1101 7 -0.13886619101209874 1101 8 -0.23559220119309998 1101 9 -0.22095517801530917 1101 10-11 -0.2599813928275765 1101 12-13 -0.14354508922912856 1101 14-15 -0.10438687000771552 1101 16-17 -0.06542482679366657 1101 18-19 0.12698028276464157 1101 20-21 0.1351948412748314 1101 22-23 -0.013948294515657267 1101 24-25 -0.014759251274405472 1101 26-27 -0.029637463830447075 1101 28-29 -0.1284590669782304 1101 30-31 -0.07934495582571088 1101 32-33 -0.3385263884516263 1101 34-35 -0.0215362102099661 1101 36-37 -0.0020253358622568385 1101 38-39 -0.06881647864435791 1101 40-41 -0.2560324292589371 1101 42-43 -0.10314087401290806 1101 44-45 -0.11367084895987034 1101 46-47 -0.05927781246183628 1101 48-49 0.0838807884469901 1101 50-51 0.15695371311446848 1101 52-53 0.22057372172998413 1101 54-55 0.09308625323965458 1101 56-57 0.09454416195237769 1101 58-59 0.0014427287262961386 1101 60-61 0.14415307593980486 1101 62-63 -0.03829300583910111 1101 64-65 -0.15156934270667222 1101 66-67 -0.05359959639375944 1101 68-69 -0.021393593065134553 1101 70-71 0.03650896016145211 1101 72-73 0.035909496363817794 1101 74-75 0.1103306167193665 1101 76-77 0.17508303331274888 1101 78-79 0.20306504717863305 1101 80-81 0.28873582755760197 1101 82-83 0.15600841942695354 1101 84-85 0.23864587304220564 1101 86-87 0.2415273397478579 1101 88-89 0.07992995184261176 1101 90-91 0.10947074733854834 1101 92-93 0.3531008882552662 1101 94-95 0.30613210062328733 1101 96-97 0.4416754299177832 1101 98-99 0.5527229346050184 1101 100-101 0.8993428621087034 1102 1 0.07037672999945244 1102 2 0.03134334998384247 1102 3 0.09568470292382614 1102 4 -0.016394052404208992 1102 5 0.17046585777259082 1102 6 0.17450039144001295 1102 7 0.22702286996194232 1102 8 0.14879781172972883 1102 9 0.12465155369297065 1102 10-11 0.18908550007078162 1102 12-13 0.18359501987504956 1102 14-15 0.3416736403946601 1102 16-17 0.3193145957236325 1102 18-19 0.38943581738499233 1102 20-21 0.24916599673348827 1102 22-23 0.1671536971782217 1102 24-25 0.16064189895593728 1102 26-27 0.05731906219771332 1102 28-29 0.15134294740457932 1102 30-31 0.04156319344775028 1102 32-33 -0.01509108644217605 1102 34-35 0.002562299225665754 1102 36-37 0.05378549761083207 1102 38-39 0.21061028048793418 1102 40-41 0.02684768438303564 1102 42-43 -0.21110981976661947 1102 44-45 -0.178920476565402 1102 46-47 0.057966172504272606 1102 48-49 -0.013441325372539836 1102 50-51 0.010391185369847733 1102 52-53 -0.05458653120231105 1102 54-55 -0.11105556246700843 1102 56-57 -0.10481877222011349 1102 58-59 -0.135868139273434 1102 60-61 0.024141928767527077 1102 62-63 -0.028799265025647003 1102 64-65 0.046012904681631994 1102 66-67 -0.06825161718866468 1102 68-69 -0.028949106969498928 1102 70-71 -0.03935593216475297 1102 72-73 0.14262423726604823 1102 74-75 -0.0366604359485585 1102 76-77 0.14324049451959908 1102 78-79 0.016251560086665506 1102 80-81 -0.07794063809825502 1102 82-83 -0.011640809642504024 1102 84-85 -0.026258784108261324 1102 86-87 0.11611958013219237 1102 88-89 -0.1291647596626504 1102 90-91 -0.05022778577249909 1102 92-93 0.01759379056460375 1102 94-95 0.21476316868022494 1102 96-97 0.457921693642124 1102 98-99 0.32438731593695636 1102 100-101 0.5873738571664617 1103 1 0.20918807852505594 1103 2 0.07094562284023809 1103 3 0.13408176496680824 1103 4 0.09715066879515177 1103 5 -0.06891317458933344 1103 6 0.08652189585520631 1103 7 0.12151654885470009 1103 8 0.1865801480812621 1103 9 0.17165856300513838 1103 10-11 0.11078486246805141 1103 12-13 0.12220483079104127 1103 14-15 0.046839002741940305 1103 16-17 0.12163123664731046 1103 18-19 -0.025218629509595303 1103 20-21 0.07233767071903685 1103 22-23 0.045420397983690464 1103 24-25 0.12535368994767992 1103 26-27 -0.09512912169125087 1103 28-29 -0.1429585870992156 1103 30-31 -0.05874428302563928 1103 32-33 0.06446059180062491 1103 34-35 0.07482097488760786 1103 36-37 0.0108863179268468 1103 38-39 -0.0660475282706372 1103 40-41 -0.011031993005289564 1103 42-43 0.07872823050377065 1103 44-45 -0.033460962482763534 1103 46-47 0.1710940695364016 1103 48-49 0.150178352212734 1103 50-51 0.038090202339006396 1103 52-53 0.08635912371348553 1103 54-55 0.20679227644835407 1103 56-57 0.1301039723274613 1103 58-59 0.10045980078570693 1103 60-61 0.07001758525139223 1103 62-63 0.17441302706412642 1103 64-65 0.17576301471109446 1103 66-67 0.26553458553370035 1103 68-69 0.46222789388118457 1103 70-71 0.49992666818172893 1103 72-73 0.2849078579890083 1103 74-75 0.29864648374925196 1103 76-77 0.3803431859045787 1103 78-79 0.38091286251731304 1103 80-81 0.3065140362031826 1103 82-83 0.2816725428964304 1103 84-85 0.34771752528018496 1103 86-87 0.43018513269218417 1103 88-89 0.551395667153173 1103 90-91 0.49904476988801605 1103 92-93 0.3639428577950632 1103 94-95 0.032536013333775315 1103 96-97 0.006649594007321014 1103 98-99 0.25798841918587456 1103 100-101 0.7487707881588612 1104 1 -0.03932727899267974 1104 2 -0.09822723098169561 1104 3 -0.1469337894774121 1104 4 -0.10064943342343469 1104 5 -0.10765310157082553 1104 6 0.050934258075365335 1104 7 -0.11286461654705704 1104 8 -0.09892386463926783 1104 9 -0.0200820655841909 1104 10-11 -0.12956702225576322 1104 12-13 -0.10323926132292627 1104 14-15 -0.020692125381174264 1104 16-17 -0.02603266572111096 1104 18-19 0.03332633109594951 1104 20-21 0.005706542412731608 1104 22-23 -0.2747379125886056 1104 24-25 -0.11859856041823491 1104 26-27 -0.13841200520786145 1104 28-29 -0.1960265184918626 1104 30-31 -0.26828266903451237 1104 32-33 -0.3559471284501967 1104 34-35 -0.20757929329878522 1104 36-37 -0.08442472516066601 1104 38-39 -0.11212905766580405 1104 40-41 -0.051825267828562005 1104 42-43 -0.01048312931479245 1104 44-45 -0.14298643377821207 1104 46-47 -0.12759385053526984 1104 48-49 -0.32748780336102357 1104 50-51 -0.32548050525906547 1104 52-53 -0.3459684190653576 1104 54-55 -0.3293794544346724 1104 56-57 -0.39282385732288816 1104 58-59 -0.37906634830321906 1104 60-61 -0.3141259860607235 1104 62-63 -0.1527265562577611 1104 64-65 -0.04635356942963398 1104 66-67 -0.17528809087120578 1104 68-69 -0.2411707824064777 1104 70-71 -0.3316128938904086 1104 72-73 -0.28611106710382117 1104 74-75 -0.2960702993330244 1104 76-77 -0.40736979237489024 1104 78-79 -0.22506014664755725 1104 80-81 -0.10314470394362729 1104 82-83 -0.10683372852505002 1104 84-85 -0.05767560409226391 1104 86-87 0.12723215140844601 1104 88-89 -0.15294917218229287 1104 90-91 0.12382700583708228 1104 92-93 0.1932196887991715 1104 94-95 0.25674304076975574 1104 96-97 0.1627279359413052 1104 98-99 0.2323302725663261 1104 100-101 0.5995351376877949 1105 1 0.0989742428300886 1105 2 0.07872794791797588 1105 3 -0.12190689396735621 1105 4 -0.017601619974961125 1105 5 -0.10524034276833305 1105 6 -0.022259467968041236 1105 7 0.038770487971383716 1105 8 0.025280847596583556 1105 9 0.031509756755824014 1105 10-11 -0.032664636926817536 1105 12-13 0.07716611279183638 1105 14-15 0.003992965600460252 1105 16-17 -0.08899478758092982 1105 18-19 -0.023975621767448274 1105 20-21 0.036779940437789094 1105 22-23 0.08909323704305194 1105 24-25 0.0809586433036813 1105 26-27 0.10029579912106357 1105 28-29 -0.029395742191262286 1105 30-31 0.04934056939863041 1105 32-33 0.06200081537915736 1105 34-35 0.052997855848396114 1105 36-37 0.046385417474596125 1105 38-39 0.17891945755955163 1105 40-41 0.06871702402513336 1105 42-43 0.16516458937597633 1105 44-45 0.13846172022797276 1105 46-47 0.03184861088481483 1105 48-49 0.10208576921766621 1105 50-51 -0.04401453965681412 1105 52-53 -0.0239502583620137 1105 54-55 -0.06236893490603279 1105 56-57 -0.20283263843974453 1105 58-59 -0.1088267645245935 1105 60-61 0.055371121336072804 1105 62-63 0.1860695045296481 1105 64-65 0.17048480102305774 1105 66-67 -0.006884793588547211 1105 68-69 0.006234602395238653 1105 70-71 -0.07840743000519979 1105 72-73 0.018622809839424548 1105 74-75 0.22210207060670228 1105 76-77 0.10813172873541532 1105 78-79 0.23465759298965594 1105 80-81 0.34541914706593957 1105 82-83 0.25554078878069575 1105 84-85 0.34378076105970123 1105 86-87 0.2526277469569713 1105 88-89 0.1948824441131798 1105 90-91 0.4291520496536094 1105 92-93 0.6285390150933594 1105 94-95 0.7096911270918795 1105 96-97 0.7130793587315978 1105 98-99 1.0602960814265252 1105 100-101 1.2852505048204108 1106 1 -0.10044068787679095 1106 2 0.017853503570378848 1106 3 -0.03418390666383431 1106 4 0.14090349347981146 1106 5 0.025398984347503983 1106 6 -0.0423599212060779 1106 7 -0.39940610054077297 1106 8 -0.22243008241300544 1106 9 -0.5192576246323668 1106 10-11 -0.6969754100463135 1106 12-13 -0.33819803927533343 1106 14-15 -0.39475118733832915 1106 16-17 -0.23989926621191415 1106 18-19 -0.48378288665021785 1106 20-21 -0.2932194784878064 1106 22-23 -0.18355722491109816 1106 24-25 -0.32348730224965294 1106 26-27 -0.5532436703580004 1106 28-29 -0.16409115025076204 1106 30-31 -0.31238060931080014 1106 32-33 -0.253826743527263 1106 34-35 -0.03791541153298539 1106 36-37 -0.32978690014067524 1106 38-39 -0.1723049751665613 1106 40-41 -0.13083373282428568 1106 42-43 -0.049951516553882414 1106 44-45 -0.07881652725161814 1106 46-47 -0.18372890444101841 1106 48-49 0.02492072229591713 1106 50-51 -0.012697534514302333 1106 52-53 0.21159079454481144 1106 54-55 -0.10741182546067307 1106 56-57 -0.16263663477897694 1106 58-59 -0.1295709392536608 1106 60-61 -0.4389005745295762 1106 62-63 -0.3062724795489089 1106 64-65 -0.16013055677350252 1106 66-67 -0.25748402657032443 1106 68-69 -0.3891055440355089 1106 70-71 -0.07288595204244785 1106 72-73 -0.2624187297172149 1106 74-75 -0.015581149417119633 1106 76-77 -0.01849836656078807 1106 78-79 -0.006550206481563237 1106 80-81 -0.39164940518718083 1106 82-83 -0.13994371832293595 1106 84-85 -0.30205892777495436 1106 86-87 -0.027775155509690563 1106 88-89 0.21115180115435095 1106 90-91 0.40906703120449706 1106 92-93 0.4461521066997385 1106 94-95 0.4657925899466946 1106 96-97 0.5018875024894065 1106 98-99 0.7090928942334038 1106 100-101 1.1766355319856423 1107 1 -0.01758508194426156 1107 2 -0.06140569876574631 1107 3 0.017497745017816868 1107 4 -0.045036207844503906 1107 5 -0.037284486028269725 1107 6 0.019870156408615003 1107 7 0.0893897190242754 1107 8 0.004788852498236906 1107 9 0.061810025666055424 1107 10-11 0.048256281339220664 1107 12-13 0.07874911613335911 1107 14-15 0.037937918427701334 1107 16-17 -0.03250537804880338 1107 18-19 0.10863623269197831 1107 20-21 0.13705537486396935 1107 22-23 0.07590638839866415 1107 24-25 0.04269779470467938 1107 26-27 0.028060922485053652 1107 28-29 0.06600055291786333 1107 30-31 0.13373225218667528 1107 32-33 0.10692148260557843 1107 34-35 0.08011527572846688 1107 36-37 -0.10904994478833885 1107 38-39 0.05049017839782266 1107 40-41 0.041568224193056835 1107 42-43 0.010681440232922057 1107 44-45 -0.06320393471595054 1107 46-47 -0.05952874562547805 1107 48-49 -0.04252209130074647 1107 50-51 -0.07901967391337195 1107 52-53 -0.197331052838571 1107 54-55 -0.21274751541174908 1107 56-57 0.03495807820035424 1107 58-59 -0.0849898792879884 1107 60-61 0.11444095573502722 1107 62-63 0.039713585667932705 1107 64-65 0.06776846727936459 1107 66-67 0.007731527106763281 1107 68-69 -0.0164359059812611 1107 70-71 0.27367261759304995 1107 72-73 0.14921322806858583 1107 74-75 0.12586330356579367 1107 76-77 0.04388668428580189 1107 78-79 0.1379873072867177 1107 80-81 0.1598721501805329 1107 82-83 0.28192671893211596 1107 84-85 0.3225280237350745 1107 86-87 0.2817614617192312 1107 88-89 0.4855799371209457 1107 90-91 0.6596920472141257 1107 92-93 0.8187525831463702 1107 94-95 0.8288436414592866 1107 96-97 0.9582567742432921 1107 98-99 1.2857534132016148 1107 100-101 1.3391320098667379 1108 1 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0.5542560644829706 2309 44-45 0.6380890265441792 2309 46-47 0.5243114292090709 2309 48-49 0.7002876318091609 2309 50-51 0.5706450375477772 2309 52-53 0.37736922343299995 2309 54-55 0.41123192915868856 2309 56-57 0.532647654102874 2309 58-59 0.566478844296121 2309 60-61 0.5506240268852665 2309 62-63 0.399182329869312 2309 64-65 0.4799272512721089 2309 66-67 0.5669933253460471 2309 68-69 0.7712563501678531 2309 70-71 0.6434731969386576 2309 72-73 0.6383955982312592 2309 74-75 0.7165445500866277 2309 76-77 0.6218432024393188 2309 78-79 0.7608740359426776 2309 80-81 0.7193697585758869 2309 82-83 0.9109986320096546 2309 84-85 0.9534645121997052 2309 86-87 1.0124727063845107 2309 88-89 0.9202875840706213 2309 90-91 1.0195331910608978 2309 92-93 1.25777310765001 2309 94-95 1.5098388891759242 2309 96-97 1.5502575267740646 2309 98-99 1.2642019112326643 2309 100-101 1.3962057991168848 2310 1 0.39063227701320713 2310 2 0.30874211023185794 2310 3 0.33210584984674085 2310 4 0.3235383139695216 2310 5 0.2061683726878698 2310 6 0.2821064641435882 2310 7 0.28122809176534247 2310 8 0.31943562285964333 2310 9 0.22220932062007392 2310 10-11 0.3619004144446478 2310 12-13 0.30880170125752926 2310 14-15 0.23576078229035602 2310 16-17 0.3153890837136615 2310 18-19 0.21860967347105031 2310 20-21 0.35654426985637855 2310 22-23 0.5008399236816317 2310 24-25 0.4752891053595647 2310 26-27 0.4359256251204471 2310 28-29 0.3656710959152676 2310 30-31 0.46614916795714834 2310 32-33 0.5019532283410371 2310 34-35 0.446289867940429 2310 36-37 0.6782162954019526 2310 38-39 0.615902926290552 2310 40-41 0.5702287168186118 2310 42-43 0.723907852258165 2310 44-45 0.8374063140500922 2310 46-47 0.8645108158807204 2310 48-49 0.87470700331248 2310 50-51 0.811121548610771 2310 52-53 0.9154336393971221 2310 54-55 1.0206816373331122 2310 56-57 0.9352330034692073 2310 58-59 0.8575164738589862 2310 60-61 0.7868385654051409 2310 62-63 0.8688999295351678 2310 64-65 1.0522275898117712 2310 66-67 1.0196678436414786 2310 68-69 0.9287137029635488 2310 70-71 0.9820685046470246 2310 72-73 1.1128642943039786 2310 74-75 1.1223160742845195 2310 76-77 0.981482514808107 2310 78-79 0.9463742612694013 2310 80-81 0.9288102613292679 2310 82-83 0.9266464738193072 2310 84-85 1.1566755554074781 2310 86-87 0.98266156864943 2310 88-89 1.1207473055448034 2310 90-91 1.242368955358156 2310 92-93 1.1890010558578368 2310 94-95 1.3684951450151814 2310 96-97 1.4316415549563928 2310 98-99 1.6603984236146445 2310 100-101 1.9366024840661566 2311 1 0.2769106573261766 2311 2 0.4025186580964295 2311 3 0.2913766224515655 2311 4 0.14751275233018646 2311 5 0.26793831003984536 2311 6 0.3084881488073705 2311 7 0.35690374108591527 2311 8 0.36086277104986664 2311 9 0.23211835641904344 2311 10-11 0.4793649420281909 2311 12-13 0.497459373893669 2311 14-15 0.5689507469973023 2311 16-17 0.6463563123156817 2311 18-19 0.5720666219040851 2311 20-21 0.4987123146031749 2311 22-23 0.5100492973662085 2311 24-25 0.6334902899675114 2311 26-27 0.5163007470998693 2311 28-29 0.6019077658254375 2311 30-31 0.5840772347513692 2311 32-33 0.5652081628337626 2311 34-35 0.4553763044789747 2311 36-37 0.5981425377578518 2311 38-39 0.6651205050719042 2311 40-41 0.7071711782872754 2311 42-43 0.7162648533719107 2311 44-45 0.8275127179493396 2311 46-47 0.7306842712550008 2311 48-49 0.7067141478296719 2311 50-51 0.8079886829261653 2311 52-53 0.7246902144448555 2311 54-55 0.7649822410601317 2311 56-57 0.7179122856462996 2311 58-59 0.7452388722640251 2311 60-61 0.7907119561648628 2311 62-63 0.6794949987224399 2311 64-65 0.6113545526559605 2311 66-67 0.8088293612268345 2311 68-69 0.7948366600767045 2311 70-71 0.7916045034062762 2311 72-73 0.9623740200368935 2311 74-75 0.7716037934092448 2311 76-77 0.9602219342615221 2311 78-79 0.9679193733975993 2311 80-81 0.881243801554561 2311 82-83 0.9216806953880301 2311 84-85 0.9787827189863201 2311 86-87 0.9090377739616606 2311 88-89 1.1390002793119436 2311 90-91 1.185455557670302 2311 92-93 1.4316636585173192 2311 94-95 1.3085313040919004 2311 96-97 1.1427480844628164 2311 98-99 1.1381310122205193 2311 100-101 1.1462829891137787 2312 1 0.4397509335006937 2312 2 0.42851855117955395 2312 3 0.4009540834342502 2312 4 0.3439387326610017 2312 5 0.3927211718521306 2312 6 0.33506903524226317 2312 7 0.48505242838754015 2312 8 0.3519957198349175 2312 9 0.5344546076431556 2312 10-11 0.5266633229424755 2312 12-13 0.5569771200389297 2312 14-15 0.5932070725697685 2312 16-17 0.6486836635910791 2312 18-19 0.6912967087828576 2312 20-21 0.7111292704447507 2312 22-23 0.7846445846818142 2312 24-25 0.7053139041456333 2312 26-27 0.713026623392345 2312 28-29 0.6720108701157912 2312 30-31 0.7608429933882874 2312 32-33 0.7221379037600784 2312 34-35 0.29449930412003766 2312 36-37 0.38469650698811364 2312 38-39 0.7186979026094065 2312 40-41 0.6769313261380461 2312 42-43 0.769640313129166 2312 44-45 0.8186596187992166 2312 46-47 0.8180750119010938 2312 48-49 0.9159529701696627 2312 50-51 1.0707088538521674 2312 52-53 0.8653521415216616 2312 54-55 0.6671919250267706 2312 56-57 0.731574000651726 2312 58-59 0.8771666599012633 2312 60-61 0.8635655264541953 2312 62-63 0.7919796294215615 2312 64-65 0.924225053408783 2312 66-67 0.9072414987595323 2312 68-69 1.036824797003156 2312 70-71 1.0500126051206493 2312 72-73 1.0161670136917884 2312 74-75 1.0005899277003678 2312 76-77 1.037075781829781 2312 78-79 1.1167748341182744 2312 80-81 0.965631910988094 2312 82-83 0.9992801706729004 2312 84-85 1.191591948145021 2312 86-87 1.0921733110750331 2312 88-89 1.0612789070397213 2312 90-91 1.0788197794065049 2312 92-93 1.2605718374820718 2312 94-95 1.3778893681210107 2312 96-97 1.4620199394177398 2312 98-99 1.385051864947222 2312 100-101 1.1783106147895737 2313 1 0.3370250608689105 2313 2 0.3300647873187721 2313 3 0.39028597373825136 2313 4 0.28109998119237645 2313 5 0.45656022521067285 2313 6 0.378732779196973 2313 7 0.2532296331002186 2313 8 0.39151330908737236 2313 9 0.3613903850629896 2313 10-11 0.3993060992100581 2313 12-13 0.3179169639758399 2313 14-15 0.376506096192486 2313 16-17 0.4739672101910841 2313 18-19 0.4062874754832677 2313 20-21 0.3722523475626289 2313 22-23 0.43247084403923 2313 24-25 0.5286358327489964 2313 26-27 0.636681463927161 2313 28-29 0.653838163762714 2313 30-31 0.5888852006633627 2313 32-33 0.5764818529999189 2313 34-35 0.5637471184170906 2313 36-37 0.5635772751346622 2313 38-39 0.5794080303161664 2313 40-41 0.6652972353698061 2313 42-43 0.8024365201123516 2313 44-45 0.6558864767383525 2313 46-47 0.8285948423362655 2313 48-49 0.8765451742125947 2313 50-51 1.0242429650796083 2313 52-53 1.0107156323020305 2313 54-55 0.9231814075288156 2313 56-57 0.9732806916117553 2313 58-59 0.8399978807827111 2313 60-61 0.8939307865893298 2313 62-63 0.9467466910330913 2313 64-65 0.9253693028058407 2313 66-67 0.8161988656397412 2313 68-69 0.9791984780674596 2313 70-71 0.9228393143140323 2313 72-73 0.9889609348418738 2313 74-75 0.7869530006331011 2313 76-77 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0.2908712620755125 2314 36-37 0.32264997256346106 2314 38-39 0.3785964990088573 2314 40-41 0.30276715033116375 2314 42-43 0.37837935821352175 2314 44-45 0.3410764046123589 2314 46-47 0.49315430885644673 2314 48-49 0.532405850382375 2314 50-51 0.6319189292001539 2314 52-53 0.4489602736180771 2314 54-55 0.5096465245041344 2314 56-57 0.6975121807707296 2314 58-59 0.6095308646588293 2314 60-61 0.4620905649916267 2314 62-63 0.5576815693717307 2314 64-65 0.630649820977915 2314 66-67 0.6756610577440227 2314 68-69 0.8171912771833618 2314 70-71 0.7257121833040117 2314 72-73 0.6358720848876267 2314 74-75 0.706290928022888 2314 76-77 0.8856243110585496 2314 78-79 0.9877671317110526 2314 80-81 0.7586687621688526 2314 82-83 1.0091697517259455 2314 84-85 1.121559213549201 2314 86-87 1.2273263688011866 2314 88-89 1.406082575650828 2314 90-91 1.2656123604112928 2314 92-93 1.366835154164523 2314 94-95 1.4543376009861397 2314 96-97 1.3180899340593193 2314 98-99 1.5217549908598684 2314 100-101 1.3654095271070759 2315 1 0.20830390086779715 2315 2 0.25597760169447525 2315 3 0.220159527679602 2315 4 0.1910622236385464 2315 5 0.3061493450978645 2315 6 0.20828942944327622 2315 7 0.3467410856064106 2315 8 0.2156651556822311 2315 9 0.25188234650760677 2315 10-11 0.30501593194502874 2315 12-13 0.39099387991657863 2315 14-15 0.39878416619702506 2315 16-17 0.3216591753465181 2315 18-19 0.33699633412901164 2315 20-21 0.41279783752950294 2315 22-23 0.22624075488687367 2315 24-25 0.48791240915006284 2315 26-27 0.6172325201181934 2315 28-29 0.5065293403697311 2315 30-31 0.48430048737029807 2315 32-33 0.5485397788392561 2315 34-35 0.29824620462863294 2315 36-37 0.4336907333369595 2315 38-39 0.6610283581667744 2315 40-41 0.583942601952053 2315 42-43 0.6520686854663182 2315 44-45 0.8337520153169251 2315 46-47 0.6870214563093455 2315 48-49 0.6352237525989466 2315 50-51 0.8294381076213355 2315 52-53 0.6678967108507337 2315 54-55 0.527393369344523 2315 56-57 0.6318510997087614 2315 58-59 0.6928677331850039 2315 60-61 0.762715587086582 2315 62-63 0.9762816330052075 2315 64-65 0.9805476539047007 2315 66-67 0.9098175607312626 2315 68-69 0.809004124074157 2315 70-71 0.913102503946007 2315 72-73 0.9313330740345869 2315 74-75 0.9131057192697476 2315 76-77 0.7365954285330076 2315 78-79 1.0263502264188666 2315 80-81 1.0261283725905983 2315 82-83 1.0709076525284331 2315 84-85 1.2546365989205732 2315 86-87 1.422686604952066 2315 88-89 1.3174865774891273 2315 90-91 1.2992834814209466 2315 92-93 1.243641284188918 2315 94-95 1.3950566592107663 2315 96-97 1.5157114563133582 2315 98-99 1.3156585507990606 2315 100-101 1.1115561669062757 2316 1 -0.10947609691219995 2316 2 -0.16892638320950937 2316 3 0.0436382711583434 2316 4 0.0213119738882952 2316 5 0.1080274669759902 2316 6 0.18051720167104435 2316 7 0.19983909870442318 2316 8 -0.03757049755341768 2316 9 -0.07405837943311866 2316 10-11 -0.25240886547977226 2316 12-13 0.13754288646558877 2316 14-15 0.2019854093982687 2316 16-17 0.16333083701818651 2316 18-19 0.2523920495247225 2316 20-21 0.1282756930073603 2316 22-23 0.1491600578061778 2316 24-25 0.27952080075845487 2316 26-27 0.34944253232820444 2316 28-29 0.3784551922955828 2316 30-31 0.27489877796858764 2316 32-33 0.2883532986527797 2316 34-35 0.3213142476966695 2316 36-37 0.33802861767483705 2316 38-39 0.549162446300862 2316 40-41 0.4752712732807254 2316 42-43 0.5558093892070275 2316 44-45 0.4087237102886192 2316 46-47 0.41507784436573303 2316 48-49 0.5293718267470169 2316 50-51 0.3456774238606499 2316 52-53 0.5608637438177624 2316 54-55 0.5340911160422692 2316 56-57 0.4237658516235072 2316 58-59 0.5112493515666259 2316 60-61 0.6608219251929199 2316 62-63 0.5863293630529327 2316 64-65 0.6599327132897699 2316 66-67 0.63073886165256 2316 68-69 0.6699076649776998 2316 70-71 0.5575447283882298 2316 72-73 0.460075442776958 2316 74-75 0.5139559801200093 2316 76-77 0.567548919486498 2316 78-79 0.572159972228615 2316 80-81 0.7638728503350762 2316 82-83 0.5493003709211486 2316 84-85 0.5404041646881375 2316 86-87 0.5507851730506381 2316 88-89 0.7009009409034874 2316 90-91 0.8874109095483753 2316 92-93 0.8717487322963642 2316 94-95 0.9343129584670642 2316 96-97 0.9555671561690602 2316 98-99 1.0219189570594658 2316 100-101 0.5037195590696655 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9039.0 3 2271.0 4 1745.0 5 1949.0 6 2188.0 7 2597.0 8 3164.0 9 4045.0 10 5065.0 11 6351.0 12 8194.0 13 9954.0 14 11194.0 15 13372.0 16 16210.0 17 20163.0 18 17751.0 19 14447.0 20 14915.0 21 15664.0 22 17591.0 23 19314.0 24 21814.0 25 25159.0 26 28848.0 27 33842.0 28 40716.0 29 48130.0 30 59422.0 31 72912.0 32 92664.0 33 118946.0 34 158251.0 35 215740.0 36 301081.0 37 393090.0 38 278092.0 39 27904.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.90443758971384 19.16477743921778 14.691995235622882 23.238789735445508 2 15.242413083016428 20.111624254084685 40.450123254520584 24.1958394083783 3 19.218853915781484 24.289098635685598 29.505412544478304 26.98663490405462 4 12.676132416537458 16.49960145776744 37.424548228776864 33.39971789691823 5 13.815992496046286 37.171109026679524 34.312128965045275 14.700769512228916 6 31.905348073462598 36.13076305069934 17.706760382065653 14.257128493772406 7 28.577073334601895 32.34366741135926 20.777252342727735 18.302006911311107 8 26.232892929422004 35.1100602634907 19.932332762706164 18.724714044381138 9 25.949169970983395 17.009358877216584 20.358740518634843 36.68273063316518 10-11 24.69214073298036 26.82819921355652 27.52451176141729 20.955148292045834 12-13 25.46110899178977 24.004117329805446 28.132135092015087 22.4026385863897 14-15 22.570920116034124 25.53852267637579 26.238828215992953 25.651728991597132 16-17 22.05618677214635 28.783453050334696 26.84550105787406 22.314859119644886 18-19 21.93307161885453 27.429733326506273 29.120018339116765 21.51717671552244 20-21 22.660863144376748 26.527581605280005 29.312844861073863 21.498710389269387 22-23 22.30864764091409 26.40895300428993 29.145762278768906 22.13663707602707 24-25 22.039841479524437 26.631345956260088 29.272414501687948 22.05639806252752 26-27 21.916692775722197 26.892194104817957 29.110297333212458 22.080815786247392 28-29 21.932578853792126 27.011257059004954 29.136246283018856 21.919917804184067 30-31 22.09079714941695 26.80617341313112 29.144200245519595 21.958829191932335 32-33 21.845451098836826 26.793752046562737 29.147821846508148 22.212975008092293 34-35 22.171772436594658 26.872466711050368 29.051394727235223 21.904366125119747 36-37 22.174499468872998 26.740195404947052 28.993287108961706 22.092018017218237 38-39 21.979076761207576 26.95558538215031 29.015311376732207 22.05002647990991 40-41 22.25354011282425 26.88860618765011 28.90021833543246 21.957635364093182 42-43 21.971346847535553 26.910294321766337 28.781713763926792 22.336645066771318 44-45 22.086722681495587 27.09481859308959 28.764176066873897 22.05428265854092 46-47 22.08572037339426 27.223920273273066 28.354993761805996 22.335365591526674 48-49 22.175656922723086 27.230551326202644 28.29982519022691 22.293966560847355 50-51 22.31975638932977 27.38782905154033 28.158650462290808 22.133764096839094 52-53 22.287692538694547 27.468810675932975 27.952115079459254 22.291381705913217 54-55 22.23596790839077 27.319848830863613 28.102334936848806 22.341848323896812 56-57 22.19657911503959 27.651390164585848 28.023934123387367 22.12809659698719 58-59 22.227912589385532 27.299391662962446 28.23771520565511 22.234980541996904 60-61 22.26520748450746 27.37380084267394 28.263788313393555 22.097203359425045 62-63 22.13291948335442 27.40309607875736 28.37960262512011 22.084381812768108 64-65 22.024559785405952 27.271092167993476 28.32424476945792 22.380103277142656 66-67 22.110967747995037 27.37613015857735 28.154728849994303 22.35817324343331 68-69 22.009639148326652 27.378867840349404 28.237796967366613 22.373696043957334 70-71 22.140043685574696 27.218573930391436 28.22066052476228 22.420721859271584 72-73 22.07356932808678 27.282512954554157 28.148419846139795 22.495497871219275 74-75 22.021623771918307 27.5791036427327 27.85202503587641 22.54724754947258 76-77 22.157731643514726 27.316576064619074 27.939642267135202 22.586050024731 78-79 22.131683262953427 27.346309288151165 27.989486343730896 22.53252110516451 80-81 22.02817352554667 27.587379447876675 27.835575686584672 22.548871339991976 82-83 22.17775975379257 27.5160190516759 27.77822263728528 22.527998557246256 84-85 22.270828392223535 27.40682730263544 27.755237185011588 22.567107120129435 86-87 22.090497072349706 27.38492595333279 27.961743396788265 22.562833577529247 88-89 22.19577742156227 27.210752719483107 27.926758417470072 22.66671144148456 90-91 22.238698846657165 27.305605319449754 27.855455902509323 22.600239931383758 92-93 22.17704589730921 27.202247993131657 27.93717605021257 22.68353005934657 94-95 22.219289493156317 27.191846428738327 27.83034120606709 22.758522872038274 96-97 22.13133272585509 27.18454969892251 27.833909498266806 22.850208076955596 98-99 22.10128853019531 27.05657409218899 27.879065159752802 22.963072217862898 100-101 22.48075849272629 26.688366243523355 28.088397520218315 22.742477743532042 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3248.0 1 1902.5 2 544.0 3 506.5 4 407.5 5 373.0 6 616.0 7 1146.0 8 1819.5 9 2236.0 10 2140.5 11 1978.0 12 2066.5 13 2203.5 14 2359.0 15 2534.0 16 2583.0 17 2674.0 18 2901.0 19 3170.5 20 3534.5 21 4122.0 22 4894.0 23 5929.0 24 7255.5 25 9019.0 26 11590.0 27 14724.0 28 17553.5 29 20887.5 30 25282.5 31 28886.0 32 32620.0 33 36853.5 34 41438.5 35 46341.0 36 52316.5 37 57858.0 38 61000.0 39 63423.5 40 65878.5 41 69463.5 42 72837.0 43 75513.0 44 78507.0 45 80928.5 46 82625.0 47 84546.0 48 84892.0 49 83873.0 50 81843.0 51 77642.0 52 74002.5 53 71977.0 54 69556.5 55 64250.0 56 56776.0 57 50004.0 58 44751.0 59 39094.5 60 32603.0 61 26688.5 62 21080.5 63 16028.5 64 12117.0 65 9134.0 66 6696.0 67 5074.0 68 3921.5 69 2826.0 70 2044.0 71 1496.0 72 1104.0 73 839.0 74 605.5 75 415.5 76 297.5 77 188.0 78 109.0 79 74.0 80 53.0 81 38.5 82 24.5 83 16.5 84 11.0 85 5.0 86 6.0 87 7.5 88 4.5 89 3.5 90 3.5 91 4.0 92 3.5 93 1.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.25794369572766696 2 0.22827829612817327 3 0.21890534206830164 4 0.22448224973392528 5 0.22438852019332653 6 0.25161695173725357 7 0.25325721869773116 8 0.24121297273079612 9 0.23516741736217892 10-11 0.24505588389534347 12-13 0.24533707251713963 14-15 0.23162912720457737 16-17 0.245454234442888 18-19 0.23507368782158022 20-21 0.22785651319547906 22-23 0.22776278365488034 24-25 0.2219749845229096 26-27 0.23001229262924952 28-29 0.244798127658697 30-31 0.24613377361222868 32-33 0.24510274866564286 34-35 0.2526011119135401 36-37 0.22661459678254606 38-39 0.22619281384985185 40-41 0.22192811975261026 42-43 0.22745816264793448 44-45 0.24629780030827647 46-47 0.25206216705509754 48-49 0.25707669747712886 50-51 0.2570532650919792 52-53 0.27872822135543224 54-55 0.2556238895978487 56-57 0.22441195257847624 58-59 0.209462090852981 60-61 0.20559574730328398 62-63 0.21206308560459539 64-65 0.20540828822208654 66-67 0.20604096262112787 68-69 0.20908717269058613 70-71 0.21051654818471655 72-73 0.21222711230064312 74-75 0.2162574825463879 76-77 0.2292624563044598 78-79 0.22300600946949548 80-81 0.21731193987812347 82-83 0.21264889523333733 84-85 0.21342216394327676 86-87 0.22038158233273145 88-89 0.21679642740483054 90-91 0.22762218934398226 92-93 0.21609345585034018 94-95 0.21428916219381486 96-97 0.22396673726063232 98-99 0.22966080685200432 100-101 0.20704855518256407 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2133799.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.25675206886899 #Duplication Level Percentage of deduplicated Percentage of total 1 80.1841577103565 49.11821068714398 2 11.575607504273817 14.181682378716811 3 3.491768633728729 6.416832164345227 4 1.5177328065901796 3.718855289603333 5 0.8383676192453544 2.567783869734032 6 0.515853276220925 1.89596977472274 7 0.3377057469731455 1.4480730050196324 8 0.22540103864665909 1.1045868432355161 9 0.17436915314107576 0.9613159184179374 >10 1.041754308983886 11.644997314722778 >50 0.0685699032320324 2.869443715947334 >100 0.027791859888299436 2.893137743524947 >500 4.6021935966970417E-4 0.17173348406996236 >1k 3.068129064463804E-4 0.43537139901282845 >5k 1.534064532231902E-4 0.5720064117830966 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6892 0.322991996903176 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5282 0.24753971672120942 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3482 0.16318313018236488 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3171 0.1486081866192645 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 2.3432385149679048E-5 0.0 22-23 0.0 0.0 0.0 9.372954059871619E-5 0.0 24-25 0.0 0.0 0.0 2.3432385149679046E-4 0.0 26-27 0.0 0.0 0.0 5.623772435922971E-4 0.0 28-29 0.0 0.0 0.0 0.001523105034729138 0.0 30-31 0.0 0.0 0.0 0.003655452083349931 0.0 32-33 0.0 0.0 0.0 0.008201334802387666 0.0 34-35 0.0 0.0 0.0 0.014270322556154541 0.0 36-37 0.0 0.0 0.0 0.02254195451399124 0.0 38-39 0.0 0.0 0.0 0.034539335710626916 0.0 40-41 0.0 0.0 0.0 0.04993441275396605 0.0 42-43 0.0 0.0 0.0 0.06743840446077629 0.0 44-45 0.0 0.0 0.0 0.08897276641333134 0.0 46-47 0.0 0.0 0.0 0.11505301108492412 0.0 48-49 0.0 0.0 0.0 0.14209398354765373 0.0 50-51 0.0 0.0 0.0 0.17541483523049733 0.0 52-53 0.0 0.0 0.0 0.21885847729800229 0.0 54-55 0.0 0.0 0.0 0.2725655040610667 0.0 56-57 0.0 0.0 0.0 0.3283345807173028 0.0 58-59 0.0 0.0 0.0 0.3977413055306521 0.0 60-61 0.0 0.0 0.0 0.4770599292623157 0.0 62-63 0.0 0.0 0.0 0.5539415849384126 0.0 64-65 0.0 0.0 0.0 0.6365407425910313 0.0 66-67 0.0 0.0 0.0 0.749508271397634 0.0 68-69 0.0 0.0 0.0 0.8747543700226685 0.0 70-71 0.0 0.0 0.0 0.9968136642673466 0.0 72-73 0.0 0.0 0.0 1.1362832206782363 0.0 74-75 0.0 0.0 0.0 1.29079636835522 0.0 76-77 0.0 0.0 0.0 1.4628135077390139 0.0 78-79 0.0 0.0 0.0 1.6660191517570304 0.0 80-81 2.3432385149679048E-5 0.0 0.0 1.895164446135742 0.0 82-83 4.6864770299358096E-5 0.0 0.0 2.1412044902073717 0.0 84-85 4.6864770299358096E-5 0.0 0.0 2.3876194524413967 0.0 86-87 4.6864770299358096E-5 0.0 0.0 2.652241377936722 0.0 88-89 4.6864770299358096E-5 0.0 0.0 2.9580574365251833 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3530 0.0 54.516445 1 GTATCAA 6595 0.0 43.590096 1 ATCAACG 8095 0.0 34.91931 3 TCAACGC 8265 0.0 34.204285 4 CAACGCA 8385 0.0 33.772236 5 TATCAAC 8450 0.0 33.395283 2 AACGCAG 8745 0.0 32.489094 6 ACGCAGA 10305 0.0 27.29353 7 CGCAGAG 10345 0.0 26.912443 8 TGGTATC 1575 0.0 25.940195 2 TAGGCGA 95 0.0012250423 25.006493 4 GCAGAGT 11325 0.0 24.373838 9 GTACATG 7545 0.0 24.372437 1 TACATGG 7385 0.0 24.123268 2 ACATGGG 7550 0.0 22.65276 3 GAGTACT 6860 0.0 21.331068 12-13 GTGGTAT 2025 0.0 21.11858 1 GTACTTT 6950 0.0 20.712065 14-15 CAGAGTA 10955 0.0 20.469652 10-11 AGAGTAC 10520 0.0 20.435429 10-11 >>END_MODULE