FastQCFastQC Report
Thu 26 May 2016
SRR937476_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937476_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2133799
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75360.3531729089759626No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65810.30841705334007563No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41610.19500430921562903No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40450.056.7508741
GTATCAA72700.040.597671
ATCAACG91600.031.7866133
TCAACGC93050.031.3423294
TATCAAC94550.030.8953322
CAACGCA94950.030.6651295
AACGCAG98650.029.6105946
ACGCAGA115050.025.393277
TGGTATC17600.025.368482
CGCAGAG115600.025.1902688
GCAGAGT126200.022.7356689
GAGTACT76500.022.29272512-13
GTACTTT79250.021.33633614-15
GTGGTAT23000.021.2840041
GGACCGA4100.020.8524826
CAGAGTA122850.020.47486110-11
AGTACTT79300.020.42731712-13
AGAGTAC117250.020.13602310-11
GTACATG75550.019.9420011
TACATGG74700.019.9022882
Produced by FastQC (version 0.11.5)