FastQCFastQC Report
Thu 26 May 2016
SRR937475_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937475_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1767351
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127590.7219279022672916No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92070.5209491493200842No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64930.3673859918035523No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT36410.2060145381421121No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN30550.1728575704543127No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT28300.16012665282674465No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT23420.13251470703895266No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21990.12442350161343163No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA150750.027.1503411
GTACATG87450.026.0922091
TACATGG88550.025.1639942
ACATGGG89400.024.2324853
GGTATCA107550.023.8236241
GAGTACT93350.023.5613612-13
TCAACGC176450.023.0743664
CAACGCA176850.022.9979255
ATCAACG177350.022.9572723
TATCAAC178000.022.8480452
AACGCAG181600.022.4417066
AGTACTT98350.022.26692412-13
CATGGGG34450.021.513254
GTACTTT103650.021.21608714-15
ACGCAGA196600.020.6115917
GTATAGC11150.020.4642521
CGCAGAG197050.020.4439778
AGAGTAC149250.020.43462610-11
GTGTAGC12800.020.4259451
TAGCCCT17350.020.262964
Produced by FastQC (version 0.11.5)