##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937475_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1767351 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.89606705176278 33.0 31.0 34.0 30.0 34.0 2 32.01788439308321 33.0 31.0 34.0 30.0 34.0 3 32.04934503672445 34.0 31.0 34.0 30.0 34.0 4 35.669874292090256 37.0 35.0 37.0 35.0 37.0 5 35.688635138124795 37.0 35.0 37.0 35.0 37.0 6 35.74353707893904 37.0 35.0 37.0 35.0 37.0 7 35.6934389377096 37.0 35.0 37.0 33.0 37.0 8 35.68077365503513 37.0 35.0 37.0 33.0 37.0 9 37.30559238091358 39.0 37.0 39.0 34.0 39.0 10-11 37.213174406215856 39.0 37.0 39.0 34.0 39.0 12-13 37.099080488256156 39.0 37.0 39.0 33.5 39.0 14-15 38.21828855728149 40.0 38.0 41.0 33.5 41.0 16-17 38.157093299520014 40.0 38.0 41.0 33.0 41.0 18-19 38.142933972934635 40.0 38.0 41.0 33.5 41.0 20-21 38.049651427475354 40.0 38.0 41.0 33.0 41.0 22-23 38.11807756354001 40.0 38.0 41.0 34.0 41.0 24-25 38.10100229099936 40.0 38.0 41.0 34.0 41.0 26-27 38.01808384412604 40.0 38.0 41.0 33.5 41.0 28-29 37.87811560918007 40.0 38.0 41.0 33.0 41.0 30-31 37.61002964323443 40.0 37.0 41.0 33.0 41.0 32-33 37.32832781943145 39.5 36.5 41.0 32.5 41.0 34-35 37.27393624695944 40.0 36.0 41.0 32.5 41.0 36-37 37.124588154814745 40.0 36.0 41.0 32.0 41.0 38-39 36.96582569053912 39.5 36.0 41.0 31.0 41.0 40-41 36.72185717494713 39.0 36.0 41.0 31.0 41.0 42-43 36.410938743916745 39.0 35.0 41.0 30.0 41.0 44-45 36.05880580597742 39.0 35.0 41.0 30.0 41.0 46-47 35.54607488834985 39.0 35.0 40.5 27.5 41.0 48-49 35.131679558842585 39.0 35.0 40.0 25.5 41.0 50-51 34.155708175682136 38.0 34.0 39.5 23.5 40.5 52-53 34.24259272776036 38.0 34.0 39.5 24.0 40.5 54-55 34.54987351126064 38.0 34.5 40.0 23.5 41.0 56-57 34.29960715217294 38.0 34.0 40.0 22.5 41.0 58-59 34.065450496251174 38.0 34.0 40.0 20.5 41.0 60-61 33.76140421455614 38.0 34.0 40.0 18.5 41.0 62-63 33.40775516578201 38.0 33.0 40.0 17.0 41.0 64-65 33.02068151714063 37.0 33.0 40.0 12.5 41.0 66-67 32.59623696707672 36.5 32.0 39.5 8.0 41.0 68-69 32.11177151567516 36.0 31.5 39.0 7.5 40.5 70-71 31.660233026716256 36.0 31.0 39.0 2.0 40.0 72-73 31.17360077313448 35.0 31.0 38.0 2.0 40.0 74-75 30.62280639216545 35.0 30.0 37.5 2.0 39.5 76-77 30.09386675312374 34.5 29.5 37.0 2.0 39.0 78-79 29.531122567050915 34.0 29.0 36.5 2.0 39.0 80-81 28.9203485894992 34.0 28.5 36.0 2.0 38.0 82-83 28.34121065934271 34.0 27.5 35.5 2.0 37.0 84-85 27.722757392278048 33.5 26.0 35.0 2.0 37.0 86-87 27.110592349793563 33.0 25.5 35.0 2.0 36.0 88-89 26.519508575263206 32.5 25.0 35.0 2.0 36.0 90-91 25.918749303335897 32.0 23.0 35.0 2.0 35.0 92-93 25.244935782422395 32.0 19.5 34.5 2.0 35.0 94-95 24.456528725759625 31.0 12.5 34.0 2.0 35.0 96-97 23.59442493313439 31.0 2.0 34.0 2.0 35.0 98-99 22.410628675345194 31.0 2.0 34.0 2.0 35.0 100-101 19.27443925966036 25.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.13223932866810983 1101 2 0.21595083120261194 1101 3 0.1071833026974005 1101 4 0.0789225583406008 1101 5 0.1313706553859575 1101 6 0.08847994067406972 1101 7 0.15978625450705408 1101 8 0.12665281665798744 1101 9 0.11432398672726407 1101 10-11 0.18620414844683353 1101 12-13 0.34027550782326443 1101 14-15 0.34938915664098147 1101 16-17 0.5033039697337998 1101 18-19 0.3351336214130356 1101 20-21 0.41410815975307713 1101 22-23 0.30669292343348076 1101 24-25 0.340333532428879 1101 26-27 0.3225203465225377 1101 28-29 0.21901281981600818 1101 30-31 0.3091240093290608 1101 32-33 0.36634828276663 1101 34-35 0.3713829657011729 1101 36-37 0.33835870277114566 1101 38-39 0.5071152284611316 1101 40-41 0.6039275415815339 1101 42-43 0.5247079804587216 1101 44-45 0.6449347034619137 1101 46-47 0.8095525748667214 1101 48-49 0.7103040395733231 1101 50-51 0.6345047898316523 1101 52-53 0.6415054612876574 1101 54-55 0.7894291121168635 1101 56-57 0.7821493175412897 1101 58-59 0.8025644732609862 1101 60-61 1.0534709250965122 1101 62-63 1.1596457484811182 1101 64-65 0.8761272625090584 1101 66-67 0.9239672468403626 1101 68-69 0.8668412877438101 1101 70-71 0.9337869335017217 1101 72-73 1.0048585530930971 1101 74-75 1.1108693745681286 1101 76-77 1.16018706311446 1101 78-79 1.3188248442347579 1101 80-81 1.502228037824974 1101 82-83 1.4543312980179302 1101 84-85 1.593804429127335 1101 86-87 1.61063663227214 1101 88-89 1.7635942799699933 1101 90-91 1.7686637906355749 1101 92-93 1.815013698239337 1101 94-95 1.921626216523606 1101 96-97 1.9462380967677184 1101 98-99 2.392978984509252 1101 100-101 2.270135282149475 1102 1 0.2390623639267382 1102 2 0.23896832067190132 1102 3 0.1322378157274855 1102 4 0.1461110186751995 1102 5 0.07247203340170927 1102 6 0.08702509084829302 1102 7 0.007240954113299836 1102 8 0.07209653270333405 1102 9 0.22404999975194073 1102 10-11 0.16452256065565507 1102 12-13 0.18766781079676775 1102 14-15 0.3895670352665235 1102 16-17 0.3714629534314824 1102 18-19 0.4248142412527045 1102 20-21 0.3593845593007785 1102 22-23 0.3096569826807567 1102 24-25 0.3921312130276746 1102 26-27 0.4553187931240359 1102 28-29 0.4654384572041792 1102 30-31 0.5668828917892981 1102 32-33 0.47102776719216166 1102 34-35 0.5848089675324388 1102 36-37 0.5914736391221993 1102 38-39 0.5488502464743235 1102 40-41 0.6609720921928002 1102 42-43 0.7423500657746516 1102 44-45 0.6156471561055028 1102 46-47 0.7012049867522947 1102 48-49 0.6869168464018216 1102 50-51 0.42049500685795493 1102 52-53 0.6883128725463479 1102 54-55 0.8996173278332833 1102 56-57 0.8861824780637093 1102 58-59 0.9331425057300322 1102 60-61 0.8590018093839333 1102 62-63 0.7888989849959174 1102 64-65 0.7381427351204408 1102 66-67 0.9180195472010908 1102 68-69 0.9925840926229696 1102 70-71 0.9323326681877262 1102 72-73 1.101280201188363 1102 74-75 1.095427596179345 1102 76-77 1.1326606497576641 1102 78-79 1.1038643528473955 1102 80-81 1.1172197713670968 1102 82-83 1.0534956215229876 1102 84-85 1.2197906831637582 1102 86-87 1.5258493989446826 1102 88-89 1.4248276173656862 1102 90-91 1.5982990640511936 1102 92-93 1.7951813595989279 1102 94-95 1.7901601645295315 1102 96-97 1.7179732404509487 1102 98-99 2.001673772622272 1102 100-101 1.813517892748603 1103 1 0.34568000132560783 1103 2 0.3227233687684077 1103 3 0.24068255532846905 1103 4 0.20401009090272026 1103 5 0.23798818074175188 1103 6 0.22244709463716106 1103 7 0.23878009476108275 1103 8 0.23606310819553045 1103 9 0.35799140373366356 1103 10-11 0.23486763762845442 1103 12-13 0.3045706574371039 1103 14-15 0.47366661512826624 1103 16-17 0.44963011669683794 1103 18-19 0.36394838595998635 1103 20-21 0.38042562985194905 1103 22-23 0.28994953580237137 1103 24-25 0.39554286575542363 1103 26-27 0.39555878955061985 1103 28-29 0.3084669603926642 1103 30-31 0.36287377696989864 1103 32-33 0.3098612923334585 1103 34-35 0.5299715970356615 1103 36-37 0.5518023992807883 1103 38-39 0.750328908495554 1103 40-41 0.8512956761639643 1103 42-43 0.6249135069145666 1103 44-45 0.8131737201943281 1103 46-47 0.6897415051487812 1103 48-49 0.6569946402949753 1103 50-51 0.6671020751126875 1103 52-53 0.7521195642794254 1103 54-55 0.9521149039587016 1103 56-57 0.8620040007561656 1103 58-59 1.0161482218700115 1103 60-61 1.0025969999963777 1103 62-63 0.9027071218574676 1103 64-65 0.690247728958937 1103 66-67 1.1208925939785814 1103 68-69 1.0907871410557917 1103 70-71 1.1055492154342375 1103 72-73 1.2194874404937543 1103 74-75 1.0967603987564019 1103 76-77 1.0122638765981655 1103 78-79 1.1158342862926602 1103 80-81 1.0945218938666947 1103 82-83 1.2557480841532787 1103 84-85 1.2919270625651613 1103 86-87 1.424927866793201 1103 88-89 1.617062383740965 1103 90-91 1.6020609962142345 1103 92-93 2.0171849514000577 1103 94-95 1.8959731281201755 1103 96-97 1.7776061384064832 1103 98-99 1.7376175991648104 1103 100-101 1.7830399690566416 1104 1 0.2810240059307638 1104 2 0.2910465333315031 1104 3 0.1752539281267005 1104 4 0.1653044076952952 1104 5 0.21936558127921302 1104 6 0.22324964856108664 1104 7 0.2868015303957634 1104 8 0.2341121149237324 1104 9 0.29637214699904746 1104 10-11 0.400109857165603 1104 12-13 0.4765663700251963 1104 14-15 0.666488655474673 1104 16-17 0.5818041223563952 1104 18-19 0.4899536266050575 1104 20-21 0.42970308196944274 1104 22-23 0.44706291355342387 1104 24-25 0.44299845278460737 1104 26-27 0.4553189566585232 1104 28-29 0.3989867075650224 1104 30-31 0.4774794034677825 1104 32-33 0.5884762759047888 1104 34-35 0.6956703069649564 1104 36-37 0.6971977060679819 1104 38-39 0.6317470244850867 1104 40-41 0.6002371812749416 1104 42-43 0.6194610916297805 1104 44-45 0.6169364620291446 1104 46-47 0.8276956445174548 1104 48-49 0.8069377526511019 1104 50-51 0.8677987973584962 1104 52-53 0.9893239408877363 1104 54-55 1.1651304549103685 1104 56-57 1.1860111920382863 1104 58-59 1.2502398077707113 1104 60-61 1.2496503839028392 1104 62-63 1.2413346513449994 1104 64-65 1.2181107412327066 1104 66-67 1.2228607568460887 1104 68-69 1.2359806516610448 1104 70-71 1.0340041869227292 1104 72-73 1.363289928874 1104 74-75 1.2486885229816345 1104 76-77 1.3383296382870284 1104 78-79 1.3709534618100996 1104 80-81 1.394887762990205 1104 82-83 1.7367926011390686 1104 84-85 1.5565099981243158 1104 86-87 1.397902139472027 1104 88-89 1.5342313738837632 1104 90-91 1.694206155528132 1104 92-93 1.7852860511091002 1104 94-95 1.8265374307496174 1104 96-97 1.8790381966776657 1104 98-99 2.092786359904718 1104 100-101 1.9436781009476327 1105 1 0.1739893641112964 1105 2 0.10739187432322694 1105 3 0.1168999237777868 1105 4 0.1130706187728947 1105 5 0.12174362463257893 1105 6 0.06712663634704086 1105 7 0.16546879605574816 1105 8 0.09145167694328649 1105 9 0.26916907876481844 1105 10-11 0.2002102600789044 1105 12-13 0.2211934597443843 1105 14-15 0.3831436483384678 1105 16-17 0.32987847257821556 1105 18-19 0.2967235762312157 1105 20-21 0.368736631810485 1105 22-23 0.37966015820759935 1105 24-25 0.24405662350263668 1105 26-27 0.3737111591445341 1105 28-29 0.386699663586505 1105 30-31 0.3500377112108026 1105 32-33 0.4146360544414307 1105 34-35 0.5613504522712631 1105 36-37 0.510961383502341 1105 38-39 0.48937382889675973 1105 40-41 0.48769163673338056 1105 42-43 0.5750234798682143 1105 44-45 0.6224193968759621 1105 46-47 0.4988820840028865 1105 48-49 0.5505347802464229 1105 50-51 0.45736160845714124 1105 52-53 0.3724966729481025 1105 54-55 0.6038924173335047 1105 56-57 0.6541033643049232 1105 58-59 0.6278548480849082 1105 60-61 0.5695833524694152 1105 62-63 0.6795333864934392 1105 64-65 0.8750356751036321 1105 66-67 0.8962107489482491 1105 68-69 0.7717320394129459 1105 70-71 0.9234037289531756 1105 72-73 1.0634526702464377 1105 74-75 0.9102642154855722 1105 76-77 0.9543154073877673 1105 78-79 0.993882299926053 1105 80-81 1.09921349621467 1105 82-83 1.0921457404187365 1105 84-85 1.2509963162045459 1105 86-87 1.417763904290691 1105 88-89 1.5998032008403626 1105 90-91 1.516789388100893 1105 92-93 1.5753215368899873 1105 94-95 1.5547164195227126 1105 96-97 1.8186063023237686 1105 98-99 1.9856890981883772 1105 100-101 1.9301954742751484 1106 1 0.13646336318081254 1106 2 -0.3570953718455705 1106 3 0.021516732676289507 1106 4 0.2001072515215867 1106 5 2.51129088063351E-4 1106 6 0.03917517063338494 1106 7 0.17388120187525402 1106 8 0.153732405859067 1106 9 0.19946365602996252 1106 10-11 0.044813766221331264 1106 12-13 0.015981005636731993 1106 14-15 0.11899281217363011 1106 16-17 0.21251516192437236 1106 18-19 0.34007296249755115 1106 20-21 0.2972349058621475 1106 22-23 0.356836955912037 1106 24-25 0.350954890342436 1106 26-27 0.36355026791474643 1106 28-29 0.4245786147774666 1106 30-31 0.39806481528233206 1106 32-33 0.3829391221102938 1106 34-35 0.42561648230790183 1106 36-37 0.5586192714213354 1106 38-39 0.593331300840056 1106 40-41 0.6891466697727324 1106 42-43 0.5007320554424908 1106 44-45 0.2632247976224704 1106 46-47 0.5338854998402169 1106 48-49 0.3496678669914459 1106 50-51 0.2779641049384125 1106 52-53 0.16249832305426537 1106 54-55 0.42796444819088464 1106 56-57 0.3524455367461101 1106 58-59 0.12236089605713829 1106 60-61 0.27812698288325777 1106 62-63 0.3871437858465683 1106 64-65 0.4493216122404675 1106 66-67 0.8635933239055902 1106 68-69 0.80875242586702 1106 70-71 0.7823884394065743 1106 72-73 0.8780501129346483 1106 74-75 0.740432036377328 1106 76-77 0.8718118631870659 1106 78-79 0.8687548674402841 1106 80-81 0.924187402196246 1106 82-83 0.9252269197625438 1106 84-85 0.9625846080546303 1106 86-87 0.902894723217603 1106 88-89 1.2419641579176144 1106 90-91 1.2564894528900972 1106 92-93 1.360735225559246 1106 94-95 1.419398895591275 1106 96-97 1.2059187497290829 1106 98-99 1.2917291138506641 1106 100-101 0.916096669367473 1107 1 0.3530914605355342 1107 2 0.33087417062457547 1107 3 0.3337706032234493 1107 4 0.2582946717014494 1107 5 0.32946229773821756 1107 6 0.24821889622884896 1107 7 0.3624126114113224 1107 8 0.2352606509331423 1107 9 0.32216033451195614 1107 10-11 0.37930994485837743 1107 12-13 0.3686890965224876 1107 14-15 0.4631805724748048 1107 16-17 0.5539811181154093 1107 18-19 0.5227312356149625 1107 20-21 0.5265179334753327 1107 22-23 0.5207491679343192 1107 24-25 0.43729318433396 1107 26-27 0.515490084782769 1107 28-29 0.5880692180926488 1107 30-31 0.6242855006601147 1107 32-33 0.5354028758491225 1107 34-35 0.7422696302640048 1107 36-37 0.8150384359233414 1107 38-39 0.9215348184279932 1107 40-41 0.9278101264055962 1107 42-43 0.8805998332832488 1107 44-45 0.7784095885312254 1107 46-47 0.9447666307769182 1107 48-49 1.0801946715517445 1107 50-51 0.8624463202618742 1107 52-53 0.9941070134871879 1107 54-55 1.1229917162498282 1107 56-57 1.0797313937017563 1107 58-59 1.1487144621623813 1107 60-61 1.102901152337033 1107 62-63 1.0842442933609178 1107 64-65 1.133844300332548 1107 66-67 1.2456356273271467 1107 68-69 1.3239716040405725 1107 70-71 1.3507419855046834 1107 72-73 1.352020620749105 1107 74-75 1.221402749264925 1107 76-77 1.421753008862357 1107 78-79 1.4021221851169265 1107 80-81 1.370083313404578 1107 82-83 1.4903676532908356 1107 84-85 1.3387881623223201 1107 86-87 1.415738679228582 1107 88-89 1.4979275348553855 1107 90-91 1.3906074966590403 1107 92-93 1.4806325722795748 1107 94-95 1.4745448143022841 1107 96-97 1.576524474104108 1107 98-99 1.9200684298733783 1107 100-101 1.690755881153006 1108 1 0.20049343880354442 1108 2 0.19182950951156386 1108 3 0.29336919350443935 1108 4 0.15268228086462443 1108 5 0.13885845279317977 1108 6 0.1575415107067215 1108 7 0.11556258474955428 1108 8 0.0956809887430623 1108 9 0.20029883092939116 1108 10-11 0.1915005094169402 1108 12-13 0.1132324828144533 1108 14-15 0.2164832771436167 1108 16-17 0.26700601067377505 1108 18-19 0.25450787178245093 1108 20-21 0.31457143005167865 1108 22-23 0.32809360268714016 1108 24-25 0.3208209779047664 1108 26-27 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60-61 -0.7450925363444725 2316 62-63 -0.5852546148707134 2316 64-65 -0.36098475566170407 2316 66-67 -0.26246103156253753 2316 68-69 -0.31677117234134045 2316 70-71 -0.33368274230101136 2316 72-73 -0.3424020132542509 2316 74-75 -0.20549737636321552 2316 76-77 -0.1587424745068695 2316 78-79 -0.13806573364354335 2316 80-81 -0.09569797398475188 2316 82-83 -0.020398603588798636 2316 84-85 -0.01717132260230514 2316 86-87 0.2309325802814648 2316 88-89 0.024750638468692188 2316 90-91 0.2762802802197015 2316 92-93 0.6557380101833807 2316 94-95 0.6899858588105374 2316 96-97 0.7645479246903726 2316 98-99 1.0408490442548093 2316 100-101 1.5584070088151272 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6481.0 3 839.0 4 779.0 5 924.0 6 1118.0 7 1373.0 8 1641.0 9 2173.0 10 2844.0 11 4318.0 12 6461.0 13 9225.0 14 10725.0 15 14620.0 16 20204.0 17 27612.0 18 22337.0 19 13727.0 20 12798.0 21 12902.0 22 13979.0 23 15570.0 24 17636.0 25 20245.0 26 23550.0 27 27441.0 28 32186.0 29 38646.0 30 47359.0 31 58099.0 32 73309.0 33 95041.0 34 125754.0 35 171892.0 36 237981.0 37 320151.0 38 249457.0 39 25954.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.90357527026682 20.74167629286734 15.844603320116821 21.510145116749023 2 14.163370653787865 21.383355186972018 42.08017892938542 22.3730952298547 3 17.801558351957414 26.687046773821095 31.84902380308005 23.66237107114144 4 10.961576977391914 18.116482677271232 41.12967238218655 29.792267963150298 5 11.578046737943884 38.83959675644025 36.05907037017705 13.523286135438816 6 27.693189004625363 39.165506308472835 19.336171129910017 13.805133556991784 7 25.29835182629716 34.64358009713556 22.86480143247445 17.19326664409283 8 24.441021144235574 34.59829695856288 21.834901516152687 19.125780381048855 9 25.604031071424117 16.72176508560298 22.756233174088692 34.917970668884216 10-11 22.798935650297416 27.606378950360128 29.62170542723611 19.972979972106344 12-13 24.514015200612405 25.82515807145031 29.48531374201276 20.17551298592453 14-15 21.4253850305248 26.984197207775395 29.175170986818767 22.41524677488104 16-17 19.34929690739074 29.964710666600876 29.793652117048058 20.892340308960325 18-19 18.94533105247145 28.46997096749772 33.18495254962306 19.399745430407766 20-21 20.56024890373015 27.102762395845858 33.0177989306123 19.31918976981169 22-23 19.711265674653415 27.433759055733898 32.82930205666425 20.02567321294844 24-25 19.48062001655442 27.511242570999254 33.2635200795338 19.744617332912526 26-27 19.461772631999203 27.573689535337405 33.32262451342449 19.641913319238903 28-29 18.975528538033554 28.029807818015428 33.278874161311265 19.715789482639757 30-31 19.581464660961473 27.582086385703054 33.231641251209695 19.604807702125775 32-33 19.176549563660775 27.67213994392011 33.132910252603416 20.018400239815705 34-35 19.73770820967121 27.8156181111395 32.88292670061 19.563746978579285 36-37 19.610393624602324 27.48750790813715 32.88931500432023 20.01278346294029 38-39 19.258577514052018 28.18878129664158 32.84636121961067 19.706279969695732 40-41 19.614854634843407 27.893665402128732 32.84251682742743 19.64896313560043 42-43 19.347391858660835 28.024843924394073 32.224139769334776 20.40362444761032 44-45 19.803615830166738 28.01024992776449 31.913292439104545 20.272841802964223 46-47 19.960031784004887 28.25648512237466 31.339885239551236 20.44359785406922 48-49 19.89251626790391 28.38245491142518 31.195295672432692 20.529733148238215 50-51 19.88090551292771 28.73243847011746 30.8542876624828 20.532368354472027 52-53 20.03339452258343 29.09005599511394 30.37681741575982 20.499732066542816 54-55 20.223401769244905 28.523231832795236 30.450445033839813 20.80292136412004 56-57 20.462966428384295 28.90951507666205 29.870158223577135 20.757360271376516 58-59 20.46967717207123 28.68786534061319 30.074766985725017 20.767690501590565 60-61 20.406692514694026 28.735111774385054 30.434833146409535 20.42336256451139 62-63 20.261402143884304 28.668753770850252 30.412190790279485 20.65765329498596 64-65 20.13514337189375 28.7783940703613 30.344038680431428 20.742423877313517 66-67 20.24600093957117 28.718783853551642 29.988792622693566 21.04642258418362 68-69 20.31451606729885 28.542416744704553 30.239857697455697 20.9032094905409 70-71 20.261412891855663 28.458712402156582 30.408974716648178 20.870899989339577 72-73 20.280923979672266 28.057269002246667 30.607053465207297 21.05475355287377 74-75 19.984722972022738 28.68392430215625 30.212846313325382 21.118506412495634 76-77 19.80176878526171 28.4203434124829 30.43605989868674 21.34182790356865 78-79 19.81535810840317 28.236280597600683 30.41898175585892 21.529379538137224 80-81 19.846674352702863 28.412597235635612 30.283247257667274 21.457481153994255 82-83 19.85732877042464 28.475300468671588 30.405047930388235 21.26232283051554 84-85 19.875568804832334 28.224966067312018 30.239212693603417 21.66025243425223 86-87 19.60232499660515 28.33790185017136 30.36111836385012 21.69865478937337 88-89 20.0069352256727 27.87926696422546 30.243169539899096 21.870628270202744 90-91 19.754095571849003 27.614928352301405 30.608537615750585 22.022438460099007 92-93 19.807734020247327 27.573652103768627 30.92352033026311 21.69509354572094 94-95 19.574933669329344 27.56631224153153 30.822908592111315 22.035845497027807 96-97 19.38840003323204 27.5042482853033 30.89190314958643 22.21544853187823 98-99 19.428398215172844 27.569035018272476 30.457498066134786 22.545068700419893 100-101 19.58989048985145 26.85452390287853 31.392899800496306 22.162685806773712 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3013.0 1 1705.0 2 409.0 3 419.0 4 363.0 5 353.5 6 535.0 7 1026.5 8 1720.0 9 2130.0 10 1983.0 11 1788.5 12 1869.5 13 2007.0 14 2201.0 15 2449.5 16 2740.5 17 3074.5 18 3484.5 19 3984.5 20 4571.5 21 5354.0 22 6294.5 23 7440.5 24 8915.0 25 11191.5 26 15316.5 27 20057.5 28 23361.0 29 27259.0 30 32967.0 31 38846.0 32 45927.5 33 53536.5 34 61124.5 35 67881.0 36 73073.0 37 76156.0 38 74403.5 39 71549.5 40 69696.5 41 67888.5 42 65678.0 43 64908.0 44 64515.5 45 62489.5 46 60604.0 47 60570.5 48 65865.0 49 63352.5 50 52477.0 51 45077.0 52 39195.0 53 34704.5 54 30828.5 55 27537.5 56 24149.5 57 20409.5 58 17287.5 59 14376.0 60 11831.0 61 9960.5 62 8093.0 63 5971.0 64 4317.0 65 3306.5 66 2577.0 67 1947.5 68 1505.0 69 1189.5 70 953.0 71 746.5 72 551.5 73 459.0 74 368.0 75 288.0 76 228.0 77 157.5 78 96.5 79 66.0 80 47.0 81 28.5 82 21.5 83 15.0 84 10.0 85 4.0 86 5.5 87 7.5 88 4.5 89 1.5 90 1.5 91 2.0 92 1.0 93 0.5 94 1.0 95 0.5 96 1.0 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.32059279679022445 2 0.2754404756044498 3 0.27430883848199933 4 0.2738561836330191 5 0.27402592920138674 6 0.2402465610962395 7 0.24035972480848455 8 0.2416045256431801 9 0.2515063504646219 10-11 0.2593995193937141 12-13 0.2524399510906436 14-15 0.24197230770797654 16-17 0.23832277798807366 18-19 0.22533724200795427 20-21 0.23192902824622838 22-23 0.22827949852632554 24-25 0.23059935462734904 26-27 0.22672349748295612 28-29 0.24084067058552602 30-31 0.2553539166809536 32-33 0.24502772793859284 34-35 0.24412241824063244 36-37 0.23399426599470052 38-39 0.22083898444621358 40-41 0.21846254648906754 42-43 0.2321553556707185 44-45 0.22754393439673276 46-47 0.23764379571460337 48-49 0.24293419926205945 50-51 0.2573908635013645 52-53 0.27082905433046406 54-55 0.24550867371563428 56-57 0.22049949330947843 58-59 0.20774028475384912 60-61 0.2098621043584438 62-63 0.21631243595641161 64-65 0.20757053918548157 66-67 0.2139077070712043 68-69 0.2141340344956944 70-71 0.21608610853192148 72-73 0.21778356421559725 74-75 0.22174429414417396 76-77 0.2323816830952086 78-79 0.23198561010235094 80-81 0.22519578736764798 82-83 0.2181796372084549 84-85 0.20171431707680024 86-87 0.20649548391915357 88-89 0.22010342031662075 90-91 0.21846254648906754 92-93 0.22115018465488748 94-95 0.21716116379824948 96-97 0.22590306056917953 98-99 0.228760444303367 100-101 0.21073912312834292 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1767351.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.32943081803046 #Duplication Level Percentage of deduplicated Percentage of total 1 76.66148837564327 43.182980058630434 2 13.647899400271255 15.375568101580377 3 4.5166068401529325 7.632536776039131 4 1.9573075522917711 4.4101608142571145 5 0.9658151527764152 2.72019089156623 6 0.5534026565946076 1.870371399549613 7 0.3459773605303518 1.3642095456219463 8 0.2340253224632704 1.0546010569089648 9 0.1641747737012339 0.8323084401542514 >10 0.8135960191389952 8.628375306610392 >50 0.08394602858748441 3.235452777436025 >100 0.05092102754610322 5.3952746751359735 >500 0.0030246814389562372 1.184528707871762 >1k 0.0015123407194781186 1.4971734092590203 >5k 2.0164542926374916E-4 0.8916479222125264 >10k+ 1.0082271463187458E-4 0.7246201171662181 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12759 0.7219279022672916 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9207 0.5209491493200842 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6493 0.3673859918035523 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3641 0.2060145381421121 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3055 0.1728575704543127 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2830 0.16012665282674465 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2342 0.13251470703895266 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2199 0.12442350161343163 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 5.658185612252462E-5 14-15 0.0 0.0 0.0 0.0 5.658185612252462E-5 16-17 0.0 0.0 0.0 0.0 5.658185612252462E-5 18-19 0.0 0.0 0.0 0.0 5.658185612252462E-5 20-21 0.0 0.0 0.0 2.829092806126231E-5 5.658185612252462E-5 22-23 0.0 0.0 0.0 3.3949113673514765E-4 5.658185612252462E-5 24-25 0.0 0.0 0.0 5.941094892865084E-4 5.658185612252462E-5 26-27 0.0 0.0 0.0 0.0010467643382667054 5.658185612252462E-5 28-29 0.0 0.0 0.0 0.0024047288852072964 5.658185612252462E-5 30-31 0.0 0.0 0.0 0.005375276331639838 5.658185612252462E-5 32-33 0.0 0.0 0.0 0.00995840667756433 5.658185612252462E-5 34-35 0.0 0.0 0.0 0.014286918670937464 5.658185612252462E-5 36-37 0.0 0.0 0.0 0.019662195002577303 5.658185612252462E-5 38-39 0.0 0.0 0.0 0.028969910334732604 5.658185612252462E-5 40-41 0.0 0.0 0.0 0.039890208566379856 5.658185612252462E-5 42-43 0.0 0.0 0.0 0.050980252366394675 5.658185612252462E-5 44-45 0.0 0.0 0.0 0.06170251410161309 5.658185612252462E-5 46-47 0.0 0.0 0.0 0.07675328783020463 5.658185612252462E-5 48-49 0.0 0.0 0.0 0.08968224195420152 5.658185612252462E-5 50-51 0.0 0.0 0.0 0.10289410535881101 5.658185612252462E-5 52-53 0.0 0.0 0.0 0.13308052560017788 5.658185612252462E-5 54-55 0.0 0.0 0.0 0.17727095523186961 5.658185612252462E-5 56-57 0.0 0.0 0.0 0.2507424954069678 5.658185612252462E-5 58-59 0.0 0.0 0.0 0.3526747092116959 5.658185612252462E-5 60-61 0.0 0.0 0.0 0.47664555597614733 5.658185612252462E-5 62-63 0.0 0.0 0.0 0.5568786279578872 5.658185612252462E-5 64-65 0.0 0.0 0.0 0.6583864778416963 5.658185612252462E-5 66-67 0.0 0.0 0.0 0.8412873277577573 5.658185612252462E-5 68-69 0.0 0.0 0.0 1.0828069806167535 5.658185612252462E-5 70-71 0.0 0.0 0.0 1.3320217659084133 5.658185612252462E-5 72-73 0.0 0.0 0.0 1.6221735241047193 5.658185612252462E-5 74-75 0.0 0.0 0.0 1.9256503094178803 5.658185612252462E-5 76-77 0.0 0.0 0.0 2.2179521781468425 5.658185612252462E-5 78-79 0.0 0.0 0.0 2.5165346329054046 5.658185612252462E-5 80-81 0.0 0.0 0.0 2.797378675769556 5.658185612252462E-5 82-83 0.0 0.0 0.0 3.0665385653444055 5.658185612252462E-5 84-85 0.0 0.0 0.0 3.5166189398710275 5.658185612252462E-5 86-87 0.0 0.0 0.0 4.119357162216221 5.658185612252462E-5 88-89 0.0 0.0 0.0 4.847565650513112 5.658185612252462E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 15075 0.0 27.150341 1 GTACATG 8745 0.0 26.092209 1 TACATGG 8855 0.0 25.163994 2 ACATGGG 8940 0.0 24.232485 3 GGTATCA 10755 0.0 23.823624 1 GAGTACT 9335 0.0 23.56136 12-13 TCAACGC 17645 0.0 23.074366 4 CAACGCA 17685 0.0 22.997925 5 ATCAACG 17735 0.0 22.957272 3 TATCAAC 17800 0.0 22.848045 2 AACGCAG 18160 0.0 22.441706 6 AGTACTT 9835 0.0 22.266924 12-13 CATGGGG 3445 0.0 21.51325 4 GTACTTT 10365 0.0 21.216087 14-15 ACGCAGA 19660 0.0 20.611591 7 GTATAGC 1115 0.0 20.464252 1 CGCAGAG 19705 0.0 20.443977 8 AGAGTAC 14925 0.0 20.434626 10-11 GTGTAGC 1280 0.0 20.425945 1 TAGCCCT 1735 0.0 20.26296 4 >>END_MODULE