FastQCFastQC Report
Thu 26 May 2016
SRR937475_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937475_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1767351
Sequences flagged as poor quality0
Sequence length101
%GC41

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136480.7722291723602159No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105580.5973912369416149No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73640.4166687884862712No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT52870.2991482733197876No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24650.1394742753420232No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT22140.1252722294552695No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT20150.1140124400868871No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG88750.024.895421
GTATCAA184900.024.464421
TACATGG91550.023.761962
GAGTACT113500.023.26970712-13
AGTACTT116350.023.23046112-13
ACATGGG92600.022.6718243
GGTATCA138100.022.6051061
GTACTTT121400.021.67535414-15
CATGGGT20600.020.7515454
TCAACGC218600.020.5114774
CAACGCA219250.020.4506685
ATCAACG219200.020.4336643
TAGCCCG1404.8545515E-420.3562775
AGAGTAC178450.020.28385210-11
TATCAAC222150.020.18372
AACGCAG223300.020.1010256
TACTTTT139750.019.35606214-15
TAGCCCT16100.019.1762034
CCGTACA1250.00603092519.0008059
TTACGTA1250.006033882518.999194
Produced by FastQC (version 0.11.5)