##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937475_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1767351 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.246403798679495 33.0 31.0 34.0 31.0 34.0 2 32.43470199185108 34.0 31.0 34.0 31.0 34.0 3 32.493241580195445 34.0 31.0 34.0 31.0 34.0 4 36.124599471185974 37.0 35.0 37.0 35.0 37.0 5 36.068135871142744 37.0 35.0 37.0 35.0 37.0 6 36.11633965182921 37.0 35.0 37.0 35.0 37.0 7 36.06074571491458 37.0 35.0 37.0 35.0 37.0 8 36.04240187715966 37.0 35.0 37.0 35.0 37.0 9 37.731672995347274 39.0 37.0 39.0 35.0 39.0 10-11 37.65818787552671 39.0 37.0 39.0 35.0 39.0 12-13 37.566094963592406 39.0 37.0 39.0 35.0 39.0 14-15 38.758886321958684 40.0 38.0 41.0 35.0 41.0 16-17 38.73661344011461 40.0 38.0 41.0 35.0 41.0 18-19 38.78291352425183 40.0 38.0 41.0 35.0 41.0 20-21 38.711155282680124 40.0 38.0 41.0 34.5 41.0 22-23 38.64930084629482 40.0 38.0 41.0 34.0 41.0 24-25 38.67424467465716 40.0 38.0 41.0 34.0 41.0 26-27 38.51650096670102 40.0 38.0 41.0 34.0 41.0 28-29 38.36773510185583 40.0 38.0 41.0 34.0 41.0 30-31 38.34116086730933 40.0 38.0 41.0 34.0 41.0 32-33 38.17110183545883 40.0 37.0 41.0 34.0 41.0 34-35 38.018123451425325 40.0 37.0 41.0 34.0 41.0 36-37 37.817880262607716 40.0 37.0 41.0 33.0 41.0 38-39 37.67933308097825 40.0 37.0 41.0 33.0 41.0 40-41 37.4691691124174 40.0 37.0 41.0 33.0 41.0 42-43 37.20929175924873 40.0 36.5 41.0 32.5 41.0 44-45 36.805882645835496 40.0 36.0 41.0 31.5 41.0 46-47 36.30933272451256 40.0 36.0 41.0 30.5 41.0 48-49 35.872705535006915 39.0 36.0 41.0 29.0 41.0 50-51 35.58238007051231 39.0 35.0 41.0 27.5 41.0 52-53 35.359301010382204 39.0 35.0 40.5 27.0 41.0 54-55 35.151108919507216 39.0 35.0 40.0 26.5 41.0 56-57 34.86589449407616 39.0 35.0 40.0 25.5 41.0 58-59 34.63179781492188 38.0 34.5 40.0 25.0 41.0 60-61 34.316161588727994 38.0 34.0 40.0 23.5 41.0 62-63 33.97354430444207 38.0 34.0 40.0 22.5 41.0 64-65 33.59905870424154 37.5 33.5 40.0 21.5 41.0 66-67 33.18546202763345 37.0 33.0 39.5 19.5 41.0 68-69 32.751002489035855 36.0 32.5 39.0 18.0 40.0 70-71 32.33566309125918 36.0 32.0 39.0 16.5 40.0 72-73 31.870556273202098 35.0 31.5 38.0 12.5 40.0 74-75 31.387709911613484 35.0 31.0 37.5 8.0 39.5 76-77 30.04509460769253 33.5 29.5 36.0 7.0 38.5 78-79 30.667635913862043 34.0 31.0 36.0 6.5 39.0 80-81 30.456318524164132 34.0 31.0 36.0 2.0 38.5 82-83 30.043005322655205 34.0 30.0 36.0 2.0 37.0 84-85 29.585510744611568 34.0 30.0 35.0 2.0 37.0 86-87 29.21246543555864 34.0 29.5 35.0 2.0 36.0 88-89 28.7577215844504 34.0 29.0 35.0 2.0 36.0 90-91 28.21461356572633 34.0 29.0 35.0 2.0 35.5 92-93 27.755215574042733 33.5 27.5 35.0 2.0 35.0 94-95 27.285248091635445 33.0 26.5 35.0 2.0 35.0 96-97 26.653974790519825 33.0 25.5 35.0 2.0 35.0 98-99 25.752863749193 32.5 24.0 34.0 2.0 35.0 100-101 22.895035564525664 29.5 9.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.22355977014910167 1101 2 0.21935363995299184 1101 3 0.24332867123098367 1101 4 0.14163249539144118 1101 5 0.182363931777779 1101 6 0.19975119209247083 1101 7 0.24665475398580128 1101 8 0.15947916158258124 1101 9 0.20781868068345943 1101 10-11 0.24130143030299678 1101 12-13 0.2356497607548249 1101 14-15 0.36681902439252667 1101 16-17 0.3142703898601127 1101 18-19 0.3551162365885503 1101 20-21 0.4188012274675046 1101 22-23 0.42699392327093477 1101 24-25 0.3527528027232876 1101 26-27 0.28247488718602654 1101 28-29 0.17305151012953957 1101 30-31 0.37272869208009496 1101 32-33 0.3409106744391295 1101 34-35 0.3664689935440748 1101 36-37 0.4413470441726375 1101 38-39 0.3615990171070891 1101 40-41 0.410186106347453 1101 42-43 0.347633473565665 1101 44-45 0.42081804615440177 1101 46-47 0.12936709384146639 1101 48-49 0.25704183103174927 1101 50-51 0.2902381468675088 1101 52-53 0.25555792694373736 1101 54-55 0.40183877807110235 1101 56-57 0.46019867786119306 1101 58-59 0.481148301078143 1101 60-61 0.44348864788358355 1101 62-63 0.3529425204383614 1101 64-65 0.4127499027175574 1101 66-67 0.4685962691705896 1101 68-69 0.492362718723534 1101 70-71 0.5083441558345569 1101 72-73 0.46328131913708503 1101 74-75 0.5939460920059894 1101 76-77 0.5904843550950609 1101 78-79 0.5069308339837519 1101 80-81 0.5039388083979972 1101 82-83 0.4429764983235067 1101 84-85 0.5639198239202372 1101 86-87 0.6742148841701479 1101 88-89 0.7395491702137242 1101 90-91 0.8414854455864322 1101 92-93 0.9312945660659189 1101 94-95 0.9002326782222916 1101 96-97 1.1077108473064925 1101 98-99 1.6165678911534123 1101 100-101 2.3134338952964946 1102 1 0.1386737543304548 1102 2 0.15578330754991043 1102 3 0.12597594847281357 1102 4 0.1418096024614357 1102 5 0.1093516883017287 1102 6 0.14365092026526582 1102 7 0.16084959338972737 1102 8 0.12336480886654755 1102 9 0.2845229928314552 1102 10-11 0.12410521057449841 1102 12-13 0.16057667486285965 1102 14-15 0.17968845410483425 1102 16-17 0.20337973710950052 1102 18-19 0.22907343103720734 1102 20-21 0.24286551908745224 1102 22-23 0.12154194807410335 1102 24-25 0.07742758767410152 1102 26-27 0.15405808703148693 1102 28-29 0.13990267794024192 1102 30-31 0.149722396011434 1102 32-33 0.1083207231903387 1102 34-35 0.10517878315198459 1102 36-37 0.17008024055486004 1102 38-39 0.20475609383507987 1102 40-41 0.30354558489280237 1102 42-43 0.24022402686159694 1102 44-45 -0.10565916855343005 1102 46-47 -0.054247345820698456 1102 48-49 -0.21371241420425946 1102 50-51 -0.2555621505905421 1102 52-53 -0.19752696550170867 1102 54-55 -0.10163409868254547 1102 56-57 -0.22420762913782966 1102 58-59 -0.1833979029708317 1102 60-61 -0.15193889179819564 1102 62-63 -0.19836811571796176 1102 64-65 -0.09797895717175464 1102 66-67 -0.06453371251373596 1102 68-69 -0.1644186846834117 1102 70-71 -0.1455623263718806 1102 72-73 -0.03252963104818818 1102 74-75 -0.09559865806992107 1102 76-77 -0.2263233688774342 1102 78-79 -0.35515609519300284 1102 80-81 -0.16817390067090798 1102 82-83 -0.07259650296037634 1102 84-85 0.0017917743396083097 1102 86-87 0.054464347953654624 1102 88-89 0.11976005621960795 1102 90-91 0.15739185239084819 1102 92-93 0.38946997162210906 1102 94-95 0.4194330134982529 1102 96-97 0.6778145601545589 1102 98-99 1.058581399805206 1102 100-101 1.8373344359406651 1103 1 0.17290260750277753 1103 2 0.2010125443381554 1103 3 0.10600854268862037 1103 4 0.08368784907391813 1103 5 0.026338253861958094 1103 6 0.1582890863709565 1103 7 0.14099976851302642 1103 8 0.12185957618772392 1103 9 0.11725462721921076 1103 10-11 0.20142286820517086 1103 12-13 0.15875367813935526 1103 14-15 0.2638006788407097 1103 16-17 0.21102939330030068 1103 18-19 0.2503897328746234 1103 20-21 0.388974904802744 1103 22-23 0.326322564434534 1103 24-25 0.24644800733940286 1103 26-27 0.2704736954699811 1103 28-29 0.3484933426730663 1103 30-31 0.2089762761813816 1103 32-33 0.08363480872618112 1103 34-35 0.12761029719995065 1103 36-37 0.18795746506739164 1103 38-39 0.19440848131868904 1103 40-41 0.12125560147236314 1103 42-43 0.07321332108712397 1103 44-45 -0.10075952615704864 1103 46-47 -0.10195727700630641 1103 48-49 -0.2330165388636658 1103 50-51 -0.4075044361006306 1103 52-53 -0.36976164648302756 1103 54-55 -0.4275013728286652 1103 56-57 -0.3531871516673917 1103 58-59 -0.5541820690922918 1103 60-61 -0.4166893837987544 1103 62-63 -0.2769854036584931 1103 64-65 -0.22826139439405324 1103 66-67 -0.1944105284490405 1103 68-69 -0.2503445456515365 1103 70-71 -0.351735521077984 1103 72-73 -0.4239428418871789 1103 74-75 -0.35918483633571796 1103 76-77 -0.4677230480589145 1103 78-79 -0.42203696931179735 1103 80-81 -0.4555910685344031 1103 82-83 -0.49558248652794745 1103 84-85 -0.47081023202421335 1103 86-87 -0.4158394114823807 1103 88-89 -0.21848835610970596 1103 90-91 -0.0750715136023885 1103 92-93 0.2072065917030912 1103 94-95 0.20461220286000525 1103 96-97 0.33842137616868584 1103 98-99 0.7495818950901487 1103 100-101 1.1713341705625275 1104 1 0.1330965740640977 1104 2 0.22349901526667537 1104 3 0.16078917082509747 1104 4 0.10076767843236212 1104 5 0.06528830102491412 1104 6 0.057715507885248485 1104 7 0.06690655114451971 1104 8 0.10095862124472887 1104 9 0.2227671360889758 1104 10-11 0.12287232405009973 1104 12-13 0.18794466246301766 1104 14-15 0.2611018107516472 1104 16-17 0.20982217836546369 1104 18-19 0.19493478845851797 1104 20-21 0.343358281214428 1104 22-23 0.23538141411437152 1104 24-25 0.22331981386584943 1104 26-27 0.24907653208667568 1104 28-29 0.18087837050062916 1104 30-31 0.24517028263885265 1104 32-33 0.2821497099404482 1104 34-35 0.28372377754111255 1104 36-37 0.3541402721039262 1104 38-39 0.13514861913140663 1104 40-41 0.22321646191846156 1104 42-43 0.11277979948193462 1104 44-45 0.17974678455991722 1104 46-47 0.21966736570936973 1104 48-49 0.18243157333572668 1104 50-51 0.07828451750869192 1104 52-53 0.16837017326614045 1104 54-55 0.1656162552878655 1104 56-57 0.0719464648336654 1104 58-59 0.038707937205607834 1104 60-61 0.171295532604546 1104 62-63 0.24302144993116315 1104 64-65 0.2063621640010922 1104 66-67 0.2427646028520627 1104 68-69 0.43666672643739446 1104 70-71 0.435078656875973 1104 72-73 0.42761406997936646 1104 74-75 0.39944199496908794 1104 76-77 0.48659068818760076 1104 78-79 0.3695537046767612 1104 80-81 0.4436766588950576 1104 82-83 0.2027710489839052 1104 84-85 0.4842435422946352 1104 86-87 0.4922393107577321 1104 88-89 0.48484917106955194 1104 90-91 0.6895283422015055 1104 92-93 0.8426691805076594 1104 94-95 0.7474401920082521 1104 96-97 0.999074469118181 1104 98-99 1.1655946091401468 1104 100-101 1.8312767913410184 1105 1 0.13066811817576962 1105 2 0.16159397024895128 1105 3 0.13431924790034344 1105 4 0.09020356111081895 1105 5 0.12627410002841088 1105 6 0.01913426367011084 1105 7 -0.038095054809581086 1105 8 0.0933432678310524 1105 9 0.25692769702253315 1105 10-11 0.16175417894994837 1105 12-13 0.0816922416587218 1105 14-15 0.23362099864898056 1105 16-17 0.24110574201453971 1105 18-19 0.32078160822965884 1105 20-21 0.3468774842390374 1105 22-23 0.24417810243649996 1105 24-25 0.21219123797950346 1105 26-27 0.21124303276370426 1105 28-29 0.3264752594878644 1105 30-31 0.3027797082701582 1105 32-33 0.3803976896572223 1105 34-35 0.2961534752734707 1105 36-37 0.1876992246911655 1105 38-39 0.334992104568947 1105 40-41 0.13075167593603254 1105 42-43 0.014616954681123673 1105 44-45 0.016434973004997744 1105 46-47 0.13580015084889396 1105 48-49 0.2100502899303862 1105 50-51 0.10957705198568846 1105 52-53 -0.07763525777862412 1105 54-55 -0.0680604438133301 1105 56-57 0.039841806581748074 1105 58-59 0.09607602997592579 1105 60-61 0.12446833854232864 1105 62-63 0.1266026185099065 1105 64-65 0.18030261247916712 1105 66-67 0.2881949862371016 1105 68-69 0.2387137990099859 1105 70-71 0.23165669059010696 1105 72-73 0.14031714929520334 1105 74-75 0.1645619736361077 1105 76-77 0.23391799711160033 1105 78-79 0.15518820781777976 1105 80-81 0.2893064760977282 1105 82-83 0.26466958575391786 1105 84-85 0.36534207822033693 1105 86-87 0.4022305967417452 1105 88-89 0.3575371160709899 1105 90-91 0.4880055529565084 1105 92-93 0.43421489637445276 1105 94-95 0.6217560784298293 1105 96-97 0.9797147188660311 1105 98-99 1.1151790839087248 1105 100-101 1.5890134268302454 1106 1 0.15618675516456904 1106 2 0.27139462096302225 1106 3 0.19879920381389837 1106 4 0.19860769680206403 1106 5 0.1339762938857021 1106 6 0.1861022739517253 1106 7 0.16691828910115447 1106 8 0.1150623492118541 1106 9 0.10581677578903737 1106 10-11 0.0317148390080888 1106 12-13 -0.0850537293973943 1106 14-15 0.13257316164027344 1106 16-17 0.23382214990980543 1106 18-19 0.2550783788425619 1106 20-21 0.28374230605304973 1106 22-23 0.2584376065188465 1106 24-25 0.2754857435179403 1106 26-27 0.2737216960287725 1106 28-29 0.20921130795787235 1106 30-31 0.18835758446203243 1106 32-33 0.2790016057785252 1106 34-35 -0.025095933373165735 1106 36-37 0.38305141261043474 1106 38-39 0.4103110238395544 1106 40-41 0.3589817391456833 1106 42-43 0.41359635814227147 1106 44-45 0.25078414722680975 1106 46-47 0.40626313301010697 1106 48-49 0.2904768952857424 1106 50-51 0.3770208494090994 1106 52-53 0.32410993525832055 1106 54-55 0.36860801144820954 1106 56-57 0.2609097819406898 1106 58-59 0.36777424006148607 1106 60-61 0.27613445582684903 1106 62-63 0.45852519599925046 1106 64-65 0.41569182392047566 1106 66-67 0.3157503872775749 1106 68-69 0.4051993198183723 1106 70-71 0.6036568583443582 1106 72-73 0.5664288446283869 1106 74-75 0.4384088492717986 1106 76-77 0.1400306801567801 1106 78-79 0.443227760196109 1106 80-81 0.49448620244663033 1106 82-83 0.36417941407625065 1106 84-85 0.49332642847241814 1106 86-87 0.4755162795035446 1106 88-89 0.7375632277271293 1106 90-91 0.6899373922055965 1106 92-93 0.7669868277943159 1106 94-95 0.42019169156185043 1106 96-97 0.6074488095497479 1106 98-99 0.822140219401259 1106 100-101 1.3131766250730585 1107 1 0.19450583897112494 1107 2 0.13915120414436188 1107 3 0.11667491281677655 1107 4 0.10637605084833979 1107 5 0.11860581174707363 1107 6 0.19239201729351407 1107 7 0.14541478618511405 1107 8 0.14603547962837382 1107 9 0.18873343273001808 1107 10-11 0.1299861391051209 1107 12-13 0.22722702573860687 1107 14-15 0.2743904097921188 1107 16-17 0.24280910525721566 1107 18-19 0.34664853886630453 1107 20-21 0.37185581653846356 1107 22-23 0.32975179191163306 1107 24-25 0.19705631202071316 1107 26-27 0.17657267120992515 1107 28-29 0.23372692686194796 1107 30-31 0.2969986247664096 1107 32-33 0.3121863583402771 1107 34-35 0.2759266396778912 1107 36-37 0.25533231779994736 1107 38-39 0.19694659052500896 1107 40-41 0.13351486429365167 1107 42-43 0.3146823288051408 1107 44-45 0.20248817166219624 1107 46-47 0.24341942708832676 1107 48-49 0.22099544690248507 1107 50-51 0.25727730754583433 1107 52-53 0.1393584155496228 1107 54-55 0.20139670608263316 1107 56-57 0.09598304140322256 1107 58-59 0.04784721608960041 1107 60-61 0.19259507678165733 1107 62-63 0.013770108351316424 1107 64-65 0.17808853708149996 1107 66-67 0.22304607273871824 1107 68-69 0.18707881517171643 1107 70-71 0.1467497281345871 1107 72-73 0.1450068707910468 1107 74-75 0.2528255745704904 1107 76-77 0.18891540737615387 1107 78-79 0.13526208577223287 1107 80-81 0.19093251829928448 1107 82-83 0.2868253661324829 1107 84-85 0.14119416796616946 1107 86-87 0.4070128882940125 1107 88-89 0.5686350600139782 1107 90-91 0.5643261325229751 1107 92-93 0.487013220541062 1107 94-95 0.6228502231047699 1107 96-97 0.6558187698355837 1107 98-99 0.8859936187803683 1107 100-101 1.6821478081283487 1108 1 0.16856049383363114 1108 2 0.19469376233234925 1108 3 0.08008825500205319 1108 4 0.0865408417741591 1108 5 0.11870998687429335 1108 6 0.11842326276895676 1108 7 0.0889192021511036 1108 8 0.08030582997461977 1108 9 0.14037277843959117 1108 10-11 0.12650112796367807 1108 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88-89 0.14089554398143278 2316 90-91 0.3349183046933497 2316 92-93 0.4299028921132084 2316 94-95 0.44856548094157134 2316 96-97 0.6594156231497728 2316 98-99 0.8315659746071198 2316 100-101 1.4487767115741512 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 5.0 4 16.0 5 30.0 6 56.0 7 186.0 8 401.0 9 855.0 10 1617.0 11 2857.0 12 4929.0 13 7008.0 14 9152.0 15 13190.0 16 18974.0 17 27852.0 18 23990.0 19 12713.0 20 9140.0 21 8607.0 22 9095.0 23 10107.0 24 11372.0 25 13342.0 26 15972.0 27 19318.0 28 24238.0 29 29878.0 30 37442.0 31 47620.0 32 61866.0 33 81266.0 34 111818.0 35 161357.0 36 246677.0 37 384485.0 38 331053.0 39 28864.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.79746914261616 21.654765240476735 15.898720419790614 20.649045197116486 2 14.88566787242602 22.755921149788584 40.523812191239884 21.834598786545513 3 18.455304011483854 27.339164659425318 31.6140370531943 22.591494275896526 4 11.829851568816833 19.246318360076746 39.93140015763706 28.99242991346937 5 11.870012267050521 39.76318028942997 35.17786091609066 13.188946527428852 6 28.04462724156096 40.08649102526889 18.299760489003035 13.569121244167118 7 25.824185461744726 35.39098911308506 21.963548836648748 16.821276588521464 8 25.11606353237133 35.453229154819844 20.747435003007325 18.683272309801506 9 26.028502544203157 17.196187967189314 22.0588892642152 34.71642022439232 10-11 23.30023860568727 28.44723543880078 28.52333803528558 19.729187920226373 12-13 25.23998450699802 26.537594154079546 28.589115458796282 19.63330588012615 14-15 21.897829010762436 27.863225810832144 28.384797360569575 21.854147817835845 16-17 19.55449143944808 30.829586199911617 29.075831569393966 20.540090791246335 18-19 19.254041783437472 29.109978719563912 32.69885268970341 18.93712680729521 20-21 20.900738903295274 27.637919844920273 32.64061031522353 18.820730936560917 22-23 20.201415800816935 27.928654321553477 32.40719522400626 19.46273465362333 24-25 19.912796043343963 28.065421073685986 32.82290840925204 19.19887447371801 26-27 19.868314848203905 28.134948847733703 33.06667745512065 18.930058848941748 28-29 19.396014300963593 28.624928456988048 32.85065553627623 19.12840170577213 30-31 19.95583212813775 28.12578507458633 32.88014018744469 19.038242609831226 32-33 19.76050004837754 28.274466446638442 32.617270273160536 19.347763231823485 34-35 20.179070258256566 28.33339274428226 32.4903485498919 18.997188447569272 36-37 20.111143739981475 27.977832360408318 32.604304408122665 19.30671949148754 38-39 19.70489158771845 28.759207638779067 32.47177059672091 19.06413017678157 40-41 20.116717052809545 28.53250429597743 32.3975260149229 18.953252636290134 42-43 19.816370581606332 28.76510050783711 31.75375403073958 19.664774879816978 44-45 20.299391575301115 28.647082554625534 31.387907665200633 19.665618204872718 46-47 20.550982798549917 28.936923112613172 30.756765351081928 19.75532873775498 48-49 20.51825019478304 29.1334318989267 30.481890694038704 19.866427212251555 50-51 20.48000086004421 29.46285146527204 30.079961479072352 19.9771861956114 52-53 20.73742001447364 29.881415745938412 29.45057320249345 19.9305910370945 54-55 20.77470745765838 29.4253376946628 29.501610036715963 20.29834481096285 56-57 21.000129572450522 29.662811744809037 29.071672802968962 20.26538587977148 58-59 21.12650514810018 29.49694203358586 29.137816992776195 20.238735825537766 60-61 20.916162097964694 29.639471729158494 29.514482408983838 19.929883763892967 62-63 20.79100303222167 29.4996862536078 29.62014902529266 20.089161688877873 64-65 20.804865586971687 29.673901788609054 29.286004873961087 20.235227750458172 66-67 20.889087679810068 29.602467195254366 29.004934503672448 20.50351062126312 68-69 20.82283032629059 29.48757773639758 29.332798068974412 20.356793868337416 70-71 20.90886303852489 29.491057520549113 29.381232137815296 20.218847303110703 72-73 20.995361693158817 29.095409929286685 29.481638444052454 20.42758993350204 74-75 20.600548504513252 29.859999513396033 29.034102450503607 20.505349531587104 76-77 20.481839770368197 29.358542813510162 29.458692698847035 20.70092471727461 78-79 20.35866106958946 29.36270157993517 29.293275642472832 20.985361708002543 80-81 20.409697903811978 29.531230638396107 29.223991159650797 20.83508029814112 82-83 20.341144678115864 29.65823291900162 29.28080215283499 20.71982025004753 84-85 20.210179438446442 29.460428540631806 29.108997836870742 21.22039418405101 86-87 19.985645183101717 29.44120890530517 29.279243342154444 21.293902569438668 88-89 20.410093976804834 29.122002363989946 29.06061105009701 21.407292609108207 90-91 20.27362191691523 28.84653145712696 29.322797921890377 21.557048704067434 92-93 20.202498011119623 28.858484512761084 29.57578284934415 21.36323462677514 94-95 19.954525162234326 28.97698306674792 29.59092449660537 21.477567274412383 96-97 19.930647618950623 28.96906160689077 29.41809521707912 21.682195557079492 98-99 19.96486254805938 29.128664748535233 28.952977488832115 21.953495214573273 100-101 19.818214433855438 29.18655995545337 29.337170802263774 21.658054808427416 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 856.0 1 545.0 2 254.5 3 289.5 4 276.5 5 298.0 6 479.0 7 929.5 8 1461.0 9 1774.5 10 1813.5 11 1758.0 12 1885.0 13 2085.5 14 2268.5 15 2531.0 16 2831.5 17 3206.5 18 3681.0 19 4245.0 20 4790.5 21 5528.5 22 6544.5 23 7597.0 24 9140.5 25 11517.5 26 15382.5 27 19800.0 28 23092.5 29 27156.5 30 32577.0 31 38262.5 32 45459.0 33 53489.0 34 61249.5 35 67410.0 36 72898.5 37 76434.5 38 74803.5 39 71965.5 40 70495.5 41 68558.0 42 65840.5 43 65518.0 44 65222.5 45 62753.5 46 60436.0 47 60805.0 48 67293.5 49 64524.5 50 52411.5 51 44663.5 52 38830.5 53 34627.5 54 30963.0 55 27265.5 56 23379.5 57 19923.0 58 17071.0 59 14347.5 60 11854.0 61 9688.0 62 7771.5 63 5743.0 64 4183.5 65 3272.5 66 2658.0 67 2092.5 68 1568.5 69 1211.5 70 961.0 71 781.5 72 621.5 73 511.5 74 401.5 75 332.5 76 263.0 77 152.5 78 87.0 79 52.5 80 29.5 81 19.5 82 12.5 83 5.5 84 1.5 85 0.5 86 1.0 87 1.0 88 1.0 89 1.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04277588322862861 2 0.0 3 0.0 4 0.0 5 8.487278418378692E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005743058396436249 14-15 0.0 16-17 0.0 18-19 0.0 20-21 1.1316371224504922E-4 22-23 6.789822734702954E-4 24-25 0.0 26-27 0.001669164755614476 28-29 0.003253456727045165 30-31 1.6974556836757385E-4 32-33 1.1316371224504922E-4 34-35 0.0 36-37 0.0 38-39 2.8290928061262305E-5 40-41 0.0 42-43 0.008515569346439954 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 1.9803649642883615E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.960729928576723E-4 84-85 2.2632742449009844E-4 86-87 0.0 88-89 0.0 90-91 0.009505751828584135 92-93 0.0014145464030631155 94-95 0.0 96-97 0.0 98-99 3.394911367351477E-4 100-101 0.012108517210220267 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1767351.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.890423242522616 #Duplication Level Percentage of deduplicated Percentage of total 1 75.64053729349736 41.51941106331885 2 14.026125988093535 15.397999838787992 3 4.742180939681912 7.809009566152912 4 2.06147895411172 4.526218091869806 5 1.073510213182978 2.9462714978392164 6 0.6325998586508312 2.0834204390702467 7 0.37869578061428094 1.4550740174652759 8 0.2691984359632088 1.1821132868996522 9 0.17211564033776783 0.8502750310318086 >10 0.8592836346400264 8.826597765473498 >50 0.08540635236765237 3.209905233071458 >100 0.0535537162916122 5.607468918045109 >500 0.003333767885245124 1.2411999516508103 >1k 0.0015627036962086516 1.2373685581119664 >5k 2.0836049282782024E-4 0.7234471591031745 >10k+ 2.0836049282782024E-4 1.3842195821082477 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13648 0.7722291723602159 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10558 0.5973912369416149 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7364 0.4166687884862712 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5287 0.2991482733197876 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2465 0.1394742753420232 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2214 0.1252722294552695 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2015 0.1140124400868871 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 2.829092806126231E-5 0.0 22-23 0.0 0.0 0.0 3.3949113673514765E-4 0.0 24-25 0.0 0.0 0.0 5.941094892865084E-4 0.0 26-27 0.0 0.0 0.0 0.0010467643382667054 0.0 28-29 0.0 0.0 0.0 0.0024047288852072964 0.0 30-31 0.0 0.0 0.0 0.005403567259701101 0.0 32-33 0.0 0.0 0.0 0.010071570389809381 0.0 34-35 0.0 0.0 0.0 0.014541537023488826 0.0 36-37 0.0 0.0 0.0 0.02000168613931245 0.0 38-39 0.0 0.0 0.0 0.029592310752080374 0.0 40-41 0.0 0.0 0.0 0.04071064548015646 0.0 42-43 0.0 0.0 0.0 0.05188556206435507 0.0 44-45 0.0 0.0 0.0 0.06263611472763475 0.0 46-47 0.0 0.0 0.0 0.07734739731949114 0.0 48-49 0.0 0.0 0.0 0.09036122422767182 0.0 50-51 0.0 0.0 0.0 0.10394086969707772 0.0 52-53 0.0 0.0 0.0 0.13486285406803744 0.0 54-55 0.0 0.0 0.0 0.1797605569012607 0.0 56-57 0.0 0.0 0.0 0.2539393702778905 0.0 58-59 0.0 0.0 0.0 0.3565222754280276 0.0 60-61 0.0 0.0 0.0 0.4802102129118664 0.0 62-63 0.0 5.658185612252462E-5 0.0 0.5606696123180964 0.0 64-65 0.0 5.658185612252462E-5 0.0 0.6629696081876209 0.0 66-67 0.0 5.658185612252462E-5 0.0 0.8468323496577647 0.0 68-69 0.0 5.658185612252462E-5 0.0 1.0910113497545195 0.0 70-71 0.0 5.658185612252462E-5 0.0 1.3425459911472029 0.0 72-73 0.0 5.658185612252462E-5 0.0 1.637450625257801 0.0 74-75 0.0 5.658185612252462E-5 0.0 1.9460763594781116 0.0 76-77 0.0 5.658185612252462E-5 0.0 2.2432159769055495 0.0 78-79 0.0 5.658185612252462E-5 0.0 2.546438143866159 0.0 80-81 0.0 5.658185612252462E-5 0.0 2.8303658978889876 0.0 82-83 0.0 5.658185612252462E-5 0.0 3.1030055716153724 0.0 84-85 0.0 5.658185612252462E-5 0.0 3.5588855863945534 0.0 86-87 0.0 5.658185612252462E-5 0.0 4.17313821646068 0.0 88-89 0.0 5.658185612252462E-5 0.0 4.918377843450452 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 8875 0.0 24.89542 1 GTATCAA 18490 0.0 24.46442 1 TACATGG 9155 0.0 23.76196 2 GAGTACT 11350 0.0 23.269707 12-13 AGTACTT 11635 0.0 23.230461 12-13 ACATGGG 9260 0.0 22.671824 3 GGTATCA 13810 0.0 22.605106 1 GTACTTT 12140 0.0 21.675354 14-15 CATGGGT 2060 0.0 20.751545 4 TCAACGC 21860 0.0 20.511477 4 CAACGCA 21925 0.0 20.450668 5 ATCAACG 21920 0.0 20.433664 3 TAGCCCG 140 4.8545515E-4 20.356277 5 AGAGTAC 17845 0.0 20.283852 10-11 TATCAAC 22215 0.0 20.1837 2 AACGCAG 22330 0.0 20.101025 6 TACTTTT 13975 0.0 19.356062 14-15 TAGCCCT 1610 0.0 19.176203 4 CCGTACA 125 0.006030925 19.000805 9 TTACGTA 125 0.0060338825 18.99919 4 >>END_MODULE