FastQCFastQC Report
Thu 26 May 2016
SRR937474_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937474_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1765502
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131810.7465865232664703No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94870.5373542482534712No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66440.37632356123074345No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT36840.20866586387327796No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT29090.16476900054488752No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN25100.14216919607001297No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT23330.1321437188969483No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22150.1254600674482385No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA153100.029.2408731
CGTATAG950.001215628625.0393091
GTACATG88150.024.394511
TATCAAC183800.024.2912122
TCAACGC182350.024.2771454
CAACGCA183000.024.2408185
GGTATCA105550.024.2042711
ATCAACG183400.024.1886373
AGTACTT104800.023.95484712-13
GAGTACT101650.023.78592712-13
AACGCAG187000.023.7020936
TACATGG89600.023.5084932
CATTCCG3400.022.3550389
ACATGGG91000.022.101823
GTACTTT109850.022.09654414-15
TAGCCCT19300.021.680994
ACGCAGA202700.021.6787727
CGCAGAG202450.021.6579978
AGAGTAC159450.021.4954310-11
CATGGGG34700.020.5549094
Produced by FastQC (version 0.11.5)