##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937474_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1765502 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.894005784190558 33.0 31.0 34.0 30.0 34.0 2 32.01240666960445 33.0 31.0 34.0 30.0 34.0 3 32.043016660417265 34.0 31.0 34.0 30.0 34.0 4 35.6591779561847 37.0 35.0 37.0 35.0 37.0 5 35.684270536085485 37.0 35.0 37.0 35.0 37.0 6 35.74386888261809 37.0 35.0 37.0 35.0 37.0 7 35.69724814811878 37.0 35.0 37.0 35.0 37.0 8 35.69578567455602 37.0 35.0 37.0 33.0 37.0 9 37.34073878137776 39.0 37.0 39.0 35.0 39.0 10-11 37.23707478099713 39.0 37.0 39.0 34.0 39.0 12-13 37.137692565627226 39.0 37.0 39.0 34.0 39.0 14-15 38.3259041904229 40.0 38.0 41.0 34.0 41.0 16-17 38.30596963356598 40.0 38.0 41.0 34.0 41.0 18-19 38.33961587129326 40.0 38.0 41.0 34.0 41.0 20-21 38.314125953977964 40.0 38.0 41.0 34.0 41.0 22-23 38.172491733229414 40.0 38.0 41.0 34.0 41.0 24-25 38.032331597471995 40.0 38.0 41.0 33.0 41.0 26-27 37.879431175948824 40.0 38.0 41.0 33.0 41.0 28-29 37.924245625323564 40.0 38.0 41.0 33.0 41.0 30-31 37.74625432313303 40.0 37.0 41.0 33.0 41.0 32-33 37.507216361125614 40.0 37.0 41.0 33.0 41.0 34-35 37.360308852666265 40.0 36.5 41.0 33.0 41.0 36-37 37.32633551250579 40.0 37.0 41.0 33.0 41.0 38-39 37.21641833314264 40.0 37.0 41.0 32.0 41.0 40-41 37.027835425844884 40.0 36.0 41.0 31.5 41.0 42-43 36.73826424439055 40.0 36.0 41.0 31.0 41.0 44-45 36.415175400537635 40.0 36.0 41.0 30.0 41.0 46-47 35.949491136232076 39.5 36.0 41.0 29.0 41.0 48-49 35.55362270900854 39.0 35.0 41.0 27.0 41.0 50-51 34.66675002350607 38.5 34.0 40.0 25.0 40.5 52-53 34.74385330631175 38.5 35.0 40.0 25.5 40.5 54-55 35.075050042424195 39.0 35.0 40.5 25.0 41.0 56-57 34.89983896931298 39.0 35.0 41.0 23.5 41.0 58-59 34.70281285436097 39.0 35.0 40.0 23.0 41.0 60-61 34.457488011908225 38.5 34.5 40.0 22.5 41.0 62-63 34.16140876645849 38.0 34.0 40.0 21.5 41.0 64-65 33.848556104722626 38.0 34.0 40.0 19.5 41.0 66-67 33.50121353586685 37.5 34.0 40.0 18.5 41.0 68-69 33.06023527585921 37.0 33.0 39.5 15.0 41.0 70-71 32.64875712403611 36.0 33.0 39.0 10.5 41.0 72-73 32.27126477341855 36.0 32.0 39.0 8.0 40.0 74-75 31.822751262813636 35.5 32.0 38.5 6.5 40.0 76-77 31.383096988845097 35.0 31.5 37.5 2.0 39.5 78-79 30.908112536830885 35.0 31.0 37.0 2.0 39.0 80-81 30.413739548298445 35.0 31.0 36.5 2.0 39.0 82-83 29.92026828630044 34.5 30.0 36.0 2.0 37.5 84-85 29.46364518420257 34.0 30.0 35.5 2.0 37.0 86-87 29.044833424147917 34.0 29.0 35.0 2.0 37.0 88-89 28.63870162707264 34.0 29.0 35.0 2.0 36.0 90-91 28.225954714296556 34.0 29.0 35.0 2.0 36.0 92-93 27.814382538224255 34.0 27.5 35.0 2.0 35.5 94-95 27.338414513265917 34.0 26.5 35.0 2.0 35.0 96-97 26.77413477866352 33.5 25.5 35.0 2.0 35.0 98-99 26.100639648100085 33.0 24.5 35.0 2.0 35.0 100-101 24.218222919033792 31.0 11.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.24845982035732206 1101 2 0.18891474597089797 1101 3 0.15608987154184106 1101 4 0.12497277498992077 1101 5 0.14133426805970828 1101 6 0.1640226834523162 1101 7 0.15194631885410104 1101 8 0.34223064163878547 1101 9 0.2869508599252981 1101 10-11 0.2745094402972512 1101 12-13 0.31184372697575924 1101 14-15 0.42607022597987765 1101 16-17 0.5692879380489586 1101 18-19 0.41177926983154833 1101 20-21 0.4134263636187825 1101 22-23 0.39076038453505646 1101 24-25 0.31094076241844704 1101 26-27 0.4074994822749858 1101 28-29 0.3488572136272481 1101 30-31 0.46773110004796337 1101 32-33 0.5363040503222507 1101 34-35 0.36299546927764226 1101 36-37 0.41844751045285733 1101 38-39 0.44455911546912574 1101 40-41 0.36608458744112937 1101 42-43 0.5889375633165628 1101 44-45 0.7292027430726407 1101 46-47 0.765150016322032 1101 48-49 0.6958986583459321 1101 50-51 0.6014634666829721 1101 52-53 0.6874306912004826 1101 54-55 0.813912167157099 1101 56-57 0.7436261296165867 1101 58-59 0.8322304483369081 1101 60-61 0.6655914869505679 1101 62-63 0.6279102196675552 1101 64-65 0.7925127931043932 1101 66-67 0.9394849716178584 1101 68-69 1.0911262570471436 1101 70-71 1.0434333378416554 1101 72-73 0.9279370920705077 1101 74-75 0.9806224252044338 1101 76-77 0.9416297715436741 1101 78-79 0.8664586900413482 1101 80-81 0.9733689464695559 1101 82-83 0.8220852549435236 1101 84-85 1.0375073567180237 1101 86-87 0.971228972479441 1101 88-89 0.9034480567926515 1101 90-91 1.1536891608990842 1101 92-93 1.2910315253959368 1101 94-95 1.2071839430932592 1101 96-97 1.0995287510249447 1101 98-99 1.056910685641487 1101 100-101 1.485708843371171 1102 1 0.24904577286635643 1102 2 0.2202039695678124 1102 3 0.34063929647588154 1102 4 0.16941551174034686 1102 5 0.21170940574253905 1102 6 0.1536260288248883 1102 7 0.27999135074529136 1102 8 0.2527752252602937 1102 9 0.3606367893804574 1102 10-11 0.39331851583710176 1102 12-13 0.36866831264649846 1102 14-15 0.502277672379627 1102 16-17 0.603836722597741 1102 18-19 0.49043458887148006 1102 20-21 0.5840089861244806 1102 22-23 0.5475314980369319 1102 24-25 0.4925956459964098 1102 26-27 0.4736256637857821 1102 28-29 0.5698717759879628 1102 30-31 0.6312326625879834 1102 32-33 0.6110001875183926 1102 34-35 0.6871841011585715 1102 36-37 0.7377179835694889 1102 38-39 0.7849510952841854 1102 40-41 0.9024408593743232 1102 42-43 0.8928772134100598 1102 44-45 0.7474985498299915 1102 46-47 0.9455881679140248 1102 48-49 0.833637522046331 1102 50-51 0.6921340140842887 1102 52-53 0.7447451715918803 1102 54-55 0.8094598886663675 1102 56-57 0.706213222011371 1102 58-59 0.6745339781404383 1102 60-61 0.884912230309773 1102 62-63 0.778666514654617 1102 64-65 0.7255525097979572 1102 66-67 0.867516875611301 1102 68-69 0.8370389981521882 1102 70-71 0.8251372108916755 1102 72-73 1.011502565059054 1102 74-75 1.0403031291159017 1102 76-77 1.0714438677039198 1102 78-79 1.1522466200600476 1102 80-81 1.1754281052594848 1102 82-83 1.0853044075857525 1102 84-85 1.1478393324346179 1102 86-87 1.0897002447968696 1102 88-89 1.2872309405755367 1102 90-91 1.302625160796623 1102 92-93 1.312975927148031 1102 94-95 1.2409690682245405 1102 96-97 1.3314490742914238 1102 98-99 1.45272320645401 1102 100-101 1.9951353143361032 1103 1 0.1260505986416689 1103 2 0.04126705948335996 1103 3 0.06912379012430492 1103 4 0.050570149452362045 1103 5 0.0956832188147132 1103 6 0.07859409354705349 1103 7 0.01822497504448961 1103 8 0.18477437365288552 1103 9 0.1077643885572428 1103 10-11 0.18479090956358846 1103 12-13 0.15799838148476653 1103 14-15 0.18602350305169324 1103 16-17 0.2098511999236905 1103 18-19 0.14546262094869178 1103 20-21 0.019306339044788956 1103 22-23 -0.07162523320995717 1103 24-25 0.0317429740781634 1103 26-27 0.21002478379145373 1103 28-29 0.16297109470203708 1103 30-31 0.0354720707208358 1103 32-33 0.07985175944373424 1103 34-35 0.05701768287740805 1103 36-37 0.08072238354109373 1103 38-39 0.017263386817788273 1103 40-41 0.2270709424060442 1103 42-43 0.1252755527756122 1103 44-45 0.26805067530014526 1103 46-47 0.11970994777350796 1103 48-49 0.23933885623127082 1103 50-51 0.13934056436460907 1103 52-53 0.23087816428225238 1103 54-55 0.08503206239427641 1103 56-57 -0.034380951574604524 1103 58-59 0.07192149499516631 1103 60-61 0.0504194689727413 1103 62-63 -0.03888901724517524 1103 64-65 0.05856915162607379 1103 66-67 0.1899239084691544 1103 68-69 0.3343182898986967 1103 70-71 0.36452494064825913 1103 72-73 0.3010091650164739 1103 74-75 0.2950863240630319 1103 76-77 0.3068680746445267 1103 78-79 0.4218956868402586 1103 80-81 0.1544049344412315 1103 82-83 0.151578092916818 1103 84-85 0.2585908759730451 1103 86-87 0.2520095202208985 1103 88-89 0.27443376507344297 1103 90-91 0.3491575218781655 1103 92-93 0.22898703352798933 1103 94-95 0.30135246897679124 1103 96-97 0.4053198073834672 1103 98-99 0.3781170748806666 1103 100-101 0.7030925210638017 1104 1 0.22035166508209514 1104 2 0.045493593688824774 1104 3 0.1282624273794788 1104 4 0.11216461016557133 1104 5 0.18188426427298765 1104 6 0.11325842480954407 1104 7 0.17597058019697442 1104 8 0.2610754948293419 1104 9 0.42167737650618875 1104 10-11 0.3462220744376552 1104 12-13 0.3007828036090743 1104 14-15 0.4059442270269855 1104 16-17 0.25349452167122877 1104 18-19 0.3508892427318031 1104 20-21 0.37125631030836814 1104 22-23 0.3776682157988631 1104 24-25 0.3954776137782048 1104 26-27 0.35086333985483975 1104 28-29 0.24194743961204068 1104 30-31 0.42907981404531625 1104 32-33 0.29259658916905096 1104 34-35 0.33683376979822555 1104 36-37 0.18966260266768842 1104 38-39 0.23744000175890534 1104 40-41 0.30183261258493843 1104 42-43 0.43863981196899005 1104 44-45 0.33828658761301966 1104 46-47 0.3874754403522189 1104 48-49 0.43017513162651255 1104 50-51 0.3826919605896535 1104 52-53 0.4413318347895583 1104 54-55 0.569944986484515 1104 56-57 0.5435453737216562 1104 58-59 0.6049017808997235 1104 60-61 0.6491888204419567 1104 62-63 0.6006705401227563 1104 64-65 0.6250282774915874 1104 66-67 0.606116652295384 1104 68-69 0.6304315752467744 1104 70-71 0.678861220604837 1104 72-73 0.7148090318254603 1104 74-75 0.7111253508563244 1104 76-77 0.7430768743744949 1104 78-79 0.8406423228547482 1104 80-81 0.8538708019617829 1104 82-83 0.7815364935298597 1104 84-85 1.0551831016314743 1104 86-87 1.026255759399131 1104 88-89 0.8820074078478299 1104 90-91 0.9441222627486496 1104 92-93 0.9951875316259127 1104 94-95 1.1431233621776649 1104 96-97 1.1453628663980346 1104 98-99 1.4706237116249632 1104 100-101 1.6857352680829685 1105 1 0.09787261269319103 1105 2 0.038091415455262734 1105 3 0.16769990253648714 1105 4 0.09456702689186613 1105 5 0.10503841007154335 1105 6 0.10187278207164496 1105 7 0.06592208667602506 1105 8 0.14257609106115865 1105 9 0.17472769847137215 1105 10-11 0.18292674094531947 1105 12-13 0.09560337758156123 1105 14-15 0.19519758579954782 1105 16-17 0.16179343255444678 1105 18-19 0.18861640705329563 1105 20-21 0.08509556621105929 1105 22-23 0.07052933353476476 1105 24-25 0.16947443387519456 1105 26-27 0.27908020924031973 1105 28-29 0.23048865910484295 1105 30-31 0.11413093098625637 1105 32-33 0.20124910526730844 1105 34-35 0.2551191660936425 1105 36-37 0.24106430391580602 1105 38-39 0.48783637666947044 1105 40-41 0.475906227339685 1105 42-43 0.4019031874360337 1105 44-45 0.3526673809988168 1105 46-47 0.42187388219974054 1105 48-49 0.47241457832338796 1105 50-51 0.31692838637866316 1105 52-53 0.27135123219793655 1105 54-55 0.20981270251917294 1105 56-57 0.24331928261742775 1105 58-59 0.3335015106079666 1105 60-61 0.40053344243097655 1105 62-63 0.2957963847844951 1105 64-65 0.46060662235206706 1105 66-67 0.46610345569787626 1105 68-69 0.5235173531305506 1105 70-71 0.4870354792899505 1105 72-73 0.5591887122452093 1105 74-75 0.562805293618073 1105 76-77 0.4462685430286015 1105 78-79 0.4550041958176223 1105 80-81 0.31595840828978794 1105 82-83 0.38881306559440887 1105 84-85 0.5200990726943537 1105 86-87 0.3330508941475223 1105 88-89 0.4585945769391735 1105 90-91 0.6848827365541972 1105 92-93 0.7612118578839606 1105 94-95 0.7998630076184803 1105 96-97 0.8810356543780031 1105 98-99 1.0682513449821478 1105 100-101 1.3021526303534188 1106 1 0.1461070449276285 1106 2 0.1673124166762605 1106 3 0.18647401731059787 1106 4 0.14335823568307404 1106 5 0.15544622447935552 1106 6 0.1561096563085158 1106 7 0.1688524896064294 1106 8 0.2003831728682428 1106 9 0.26530156904524205 1106 10-11 -0.05136874135016711 1106 12-13 -0.1570559630777808 1106 14-15 0.05771529031725464 1106 16-17 0.11019147895250114 1106 18-19 0.13096072939762138 1106 20-21 0.17706870418419385 1106 22-23 0.09617035917580097 1106 24-25 0.25254403094479727 1106 26-27 0.18666262682274493 1106 28-29 0.29982931844551075 1106 30-31 0.2914960103513309 1106 32-33 0.33605985007805117 1106 34-35 0.3597198986943724 1106 36-37 0.44069556154706646 1106 38-39 0.4092087820418655 1106 40-41 0.40662417605764034 1106 42-43 0.3005112460440884 1106 44-45 0.34362464595609055 1106 46-47 0.6900936624195211 1106 48-49 0.7485073744161852 1106 50-51 0.7093459687962422 1106 52-53 0.5721399989867066 1106 54-55 0.5267772934837609 1106 56-57 0.4883546916528374 1106 58-59 0.6220656131720759 1106 60-61 0.616340524238062 1106 62-63 0.6220869830750928 1106 64-65 0.8899228616683033 1106 66-67 0.9765259221203451 1106 68-69 0.7772585008716959 1106 70-71 0.8240757723302394 1106 72-73 0.9098334008898945 1106 74-75 0.7313620701748427 1106 76-77 0.9095432683033238 1106 78-79 0.9461055389189639 1106 80-81 1.1287178710492505 1106 82-83 1.0202514050327487 1106 84-85 0.8319330165282999 1106 86-87 1.121224276320902 1106 88-89 1.2091562375008316 1106 90-91 1.2287337744052351 1106 92-93 1.2343254109975135 1106 94-95 1.3184124581679306 1106 96-97 1.356056411398761 1106 98-99 1.6112452399760464 1106 100-101 1.639144083344874 1107 1 0.1424440412646213 1107 2 0.13541183708522198 1107 3 0.18503437849843252 1107 4 0.21616784958446544 1107 5 0.15447572260903542 1107 6 0.1858919782324122 1107 7 0.16038605102601622 1107 8 0.25907656239546384 1107 9 0.16874432371774617 1107 10-11 0.2922687582561423 1107 12-13 0.3672952106435261 1107 14-15 0.46169017299639137 1107 16-17 0.42502832843241833 1107 18-19 0.30795225416958516 1107 20-21 0.14388175183108132 1107 22-23 0.3049538771974625 1107 24-25 0.38964188609903516 1107 26-27 0.5389619471784499 1107 28-29 0.41191294567610015 1107 30-31 0.4116338875377963 1107 32-33 0.4553548555458278 1107 34-35 0.5020870296637838 1107 36-37 0.5109415856023247 1107 38-39 0.6045305235543736 1107 40-41 0.4540712800434292 1107 42-43 0.24286728033174398 1107 44-45 0.3738726256641698 1107 46-47 0.47061173462925154 1107 48-49 0.5049683532826847 1107 50-51 0.33198023315216574 1107 52-53 0.42896936571409583 1107 54-55 0.5216598188102068 1107 56-57 0.4645279551686343 1107 58-59 0.611218881419525 1107 60-61 0.6215803187700004 1107 62-63 0.5309520964992913 1107 64-65 0.655169995391752 1107 66-67 0.8362025215989846 1107 68-69 0.771646070244266 1107 70-71 0.814735882066536 1107 72-73 0.7839404476124159 1107 74-75 0.7318427432709917 1107 76-77 0.7412785695947122 1107 78-79 0.8265237776155487 1107 80-81 0.8510428199788791 1107 82-83 0.752798668473833 1107 84-85 0.878051082292437 1107 86-87 0.9018111992293605 1107 88-89 0.878785681575387 1107 90-91 0.9897515342232346 1107 92-93 0.958142186104034 1107 94-95 1.1236689892792917 1107 96-97 1.2125629489190963 1107 98-99 1.4527968930153996 1107 100-101 1.7354927623270235 1108 1 0.045828320740604056 1108 2 0.016138202729120366 1108 3 0.025926352941976205 1108 4 -0.008586435366403578 1108 5 -0.03996595674068715 1108 6 0.023139157617492856 1108 7 0.09663013408712828 1108 8 0.01026355207176266 1108 9 0.16188303222059375 1108 10-11 0.10520933607944727 1108 12-13 0.17818385169260154 1108 14-15 0.28730550844458236 1108 16-17 0.25137141134421626 1108 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-2.4880429973073923 2316 98-99 -2.6750541659862854 2316 100-101 -3.385195684253322 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5894.0 3 924.0 4 807.0 5 844.0 6 1037.0 7 1269.0 8 1531.0 9 1889.0 10 2577.0 11 3870.0 12 5985.0 13 8604.0 14 9894.0 15 13946.0 16 18758.0 17 26126.0 18 21096.0 19 11787.0 20 10328.0 21 9928.0 22 10484.0 23 11574.0 24 12944.0 25 14623.0 26 16585.0 27 19546.0 28 23488.0 29 27568.0 30 34329.0 31 43051.0 32 55765.0 33 74349.0 34 102623.0 35 147231.0 36 222096.0 37 353244.0 38 376994.0 39 61906.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.92955601772723 20.727357677111762 15.789901479372508 21.553184825788495 2 14.171489538907469 21.39025979596509 42.03531552577592 22.402935139351513 3 17.74864699202482 26.679209719502573 31.83538152069746 23.736761767775146 4 10.970151076762084 18.133358633043052 41.008822137549 29.887668152645873 5 11.560207294390077 38.79532008258143 36.051998577436 13.592474045592496 6 27.64022047011676 39.24407812952336 19.319460290970603 13.796241109389278 7 25.32387485201022 34.63874602286336 22.79366109971588 17.24371802541054 8 24.475346656601374 34.61202272636974 21.74358479535483 19.169045821674054 9 25.540840042339813 16.733818216903575 22.779273923271855 34.94606781748475 10-11 22.847173654380644 27.558005140874492 29.580549828334757 20.014271376410107 12-13 24.560369856365632 25.830317891432742 29.417317292601453 20.191994959600173 14-15 21.4496793313678 27.00359363413382 29.145414334976195 22.401312699522183 16-17 19.333716264806196 30.00462015146605 29.742338579910744 20.919325003817015 18-19 18.91661341091457 28.395181896298283 33.2468565863271 19.441348106460048 20-21 20.550528224467733 27.05437179170675 33.05496201834811 19.340137965477417 22-23 19.756612495424246 27.426284261395402 32.790457461811975 20.026645781368384 24-25 19.508485805445726 27.51963091747141 33.23473540999003 19.737147867092837 26-27 19.472654387964024 27.564630027284327 33.325010328092866 19.637705256658784 28-29 18.985317517863916 27.996610403258142 33.26998177779581 19.748090301082122 30-31 19.52568153584295 27.580421811304024 33.23700398117803 19.656892671674996 32-33 19.17721095076786 27.62453910145217 33.16990922796087 20.02834071981909 34-35 19.707860557116458 27.80002208189089 32.89659629506742 19.59552106592523 36-37 19.601845320215496 27.502938290507974 32.929652983776556 19.96556340549998 38-39 19.261674905306013 28.13290328402067 32.873887670188836 19.731534140484477 40-41 19.679395382001914 27.90079053076873 32.81044598124869 19.609368105980664 42-43 19.340365911622197 27.987117186779507 32.27607244454219 20.396444457056106 44-45 19.798989869752337 27.99618150561508 31.949185874068007 20.255642750564572 46-47 19.937486590890774 28.244323335198786 31.3298941687828 20.488295905127643 48-49 19.88284143576004 28.363327780032527 31.24103501417653 20.512795770030905 50-51 19.823796423307506 28.771260896355727 30.848085792029146 20.556856888307617 52-53 20.0584337296384 29.044039058394837 30.38451744623254 20.51300976573423 54-55 20.200636699538496 28.52387821936419 30.48066359338189 20.794821487715424 56-57 20.429317354423166 28.85124297804016 29.92899092319744 20.790448744339233 58-59 20.437258689449635 28.661025778379027 30.163081103043147 20.738634429128183 60-61 20.44072301544698 28.71673496810393 30.431297757571702 20.41124425887739 62-63 20.24618978155211 28.66273412984749 30.496615897803604 20.594460190796795 64-65 20.171906763491304 28.801802197989268 30.3256695469112 20.70062149160823 66-67 20.27504629546879 28.764643681643786 29.964708482532842 20.995601540354585 68-69 20.31086248366562 28.5771229588053 30.244549012220357 20.86746554530872 70-71 20.284570584820443 28.470876444714815 30.388508902797017 20.856044067667725 72-73 20.263164241441487 28.107955504672404 30.66023723085959 20.968643023026516 74-75 20.039316088469146 28.70446167802178 30.279772445653087 20.976449787855994 76-77 19.826189453325625 28.480623304925796 30.432679129511474 21.26050811223711 78-79 19.792422142697433 28.303097267290685 30.41508194041997 21.489398649591916 80-81 19.835941188755314 28.449922592888534 30.267485835179603 21.446650383176554 82-83 19.81972933296789 28.54712102047452 30.431031205188397 21.202118441369187 84-85 19.838024288980193 28.29082317320552 30.232420903071617 21.638731634742673 86-87 19.587405696925643 28.426154709954655 30.29154761306935 21.69489198005035 88-89 20.018492715975093 28.00120069306546 30.185684685857893 21.794621905101554 90-91 19.735511180581188 27.764204378601086 30.543013835598597 21.95727060521913 92-93 19.792706392787082 27.7064466944299 30.84901800675601 21.651828906027013 94-95 19.488923672198233 27.69052160494615 30.83307728164749 21.98747744120812 96-97 19.288473989036248 27.654959023532744 30.86064483108662 22.195922156344388 98-99 19.35293673677502 27.80464164496529 30.481727998946756 22.36069361931293 100-101 19.220586599849753 27.502173138420403 31.136165562508083 22.141074699221754 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2617.0 1 1504.5 2 387.5 3 408.0 4 363.0 5 341.5 6 556.0 7 1076.5 8 1802.0 9 2195.5 10 2015.5 11 1783.5 12 1897.0 13 2155.0 14 2333.0 15 2568.0 16 2867.0 17 3183.0 18 3581.0 19 4098.5 20 4726.5 21 5416.0 22 6377.5 23 7560.0 24 9046.0 25 11375.5 26 15376.0 27 20111.0 28 23321.0 29 27095.5 30 32886.0 31 38693.5 32 45765.0 33 53323.0 34 60740.5 35 67352.5 36 72609.0 37 75942.0 38 74276.0 39 71440.5 40 69433.5 41 67434.5 42 65513.0 43 64959.0 44 64391.5 45 62410.0 46 60417.5 47 60588.0 48 67142.0 49 64229.0 50 51978.5 51 44474.5 52 38802.5 53 34686.5 54 31271.5 55 27707.5 56 23963.5 57 20467.0 58 17234.5 59 14216.5 60 11746.0 61 9860.0 62 8060.5 63 5880.0 64 4179.5 65 3292.5 66 2574.0 67 1983.0 68 1534.0 69 1188.0 70 944.0 71 703.5 72 517.0 73 484.5 74 405.5 75 283.5 76 206.0 77 130.0 78 84.5 79 59.0 80 40.0 81 26.0 82 21.5 83 14.5 84 10.5 85 10.5 86 8.5 87 4.5 88 5.0 89 5.5 90 5.0 91 6.0 92 5.5 93 4.5 94 4.0 95 2.0 96 4.0 97 6.5 98 9.5 99 8.5 100 4.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3497588787778207 2 0.3009342385338561 3 0.29255135366598284 4 0.2985553117470272 5 0.3001979040522186 6 0.2159159264617089 7 0.20141580128484704 8 0.20798617050561255 9 0.20203885353854031 10-11 0.22282614236630716 12-13 0.21166784291380014 14-15 0.20597541095960242 16-17 0.20702327156808656 18-19 0.20186893019662394 20-21 0.19637474214132863 22-23 0.19807397556049217 24-25 0.1866041499811385 26-27 0.18793521615948325 28-29 0.2062019754154909 30-31 0.2173602748679979 32-33 0.21104479066010687 34-35 0.2196259194268826 36-37 0.1938825331265555 38-39 0.19136200355479632 40-41 0.18869987119810683 42-43 0.19623313935639833 44-45 0.20113259571498646 46-47 0.20617365485850483 48-49 0.214499898612406 50-51 0.21787004489374692 52-53 0.23735458810015508 54-55 0.2129989090921449 56-57 0.18479163433403079 58-59 0.1840836204093793 60-61 0.18215782253432736 62-63 0.19062566907315878 64-65 0.18178965529350857 66-67 0.1801470629883172 68-69 0.17643707002314357 70-71 0.1870855994499015 72-73 0.1866041499811385 74-75 0.1910787979849357 76-77 0.1932028397588901 78-79 0.1907672718580891 80-81 0.19201337636547564 82-83 0.18481995489101685 84-85 0.17779645675847436 86-87 0.17929744627873545 88-89 0.1804869096721499 90-91 0.18535804547375195 92-93 0.1823277458762437 94-95 0.1791275229368191 96-97 0.17836286789819553 98-99 0.18833170395728807 100-101 0.17411478435028677 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1765502.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.42125056428482 #Duplication Level Percentage of deduplicated Percentage of total 1 76.61808396492754 43.22888113140589 2 13.781601923936712 15.551504306553257 3 4.449407474074891 7.53123401872143 4 1.9374223953157772 4.372471776598733 5 0.9906316520046032 2.794633832733156 6 0.5624591886260711 1.904079048819354 7 0.34173206350450014 1.3496665266586243 8 0.21584349924582946 0.9742528122896771 9 0.15951382784360005 0.8099972684308754 >10 0.807236789086416 8.675134075781516 >50 0.08214072578806499 3.2233715745412366 >100 0.04930754709124786 5.3362020673550425 >500 0.002811533902892786 1.1120547678875783 >1k 0.0015061788765497067 1.4758845971898367 >5k 2.0082385020662755E-4 0.9138802517089268 >10k+ 1.0041192510331378E-4 0.746751943324987 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13181 0.7465865232664703 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9487 0.5373542482534712 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6644 0.37632356123074345 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3684 0.20866586387327796 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2909 0.16476900054488752 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2510 0.14216919607001297 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2333 0.1321437188969483 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2215 0.1254600674482385 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 5.664111397211671E-5 0.0 14-15 0.0 0.0 0.0 5.664111397211671E-5 0.0 16-17 0.0 0.0 0.0 5.664111397211671E-5 0.0 18-19 0.0 0.0 0.0 5.664111397211671E-5 0.0 20-21 0.0 0.0 0.0 5.664111397211671E-5 0.0 22-23 0.0 0.0 0.0 2.8320556986058355E-4 0.0 24-25 0.0 0.0 0.0 5.947316967072255E-4 0.0 26-27 0.0 0.0 0.0 8.496167095817506E-4 0.0 28-29 0.0 0.0 0.0 0.0020957212169683184 0.0 30-31 0.0 0.0 0.0 0.004984418029546271 0.0 32-33 0.0 0.0 0.0 0.008751052108692033 0.0 34-35 0.0 0.0 0.0 0.012687609529754142 0.0 36-37 0.0 0.0 0.0 0.018833170395728807 0.0 38-39 2.8320556986058356E-5 0.0 0.0 0.027527581390448724 0.0 40-41 5.664111397211671E-5 0.0 0.0 0.0379495463613182 0.0 42-43 5.664111397211671E-5 0.0 0.0 0.048484793560131904 0.0 44-45 5.664111397211671E-5 0.0 0.0 0.05958645189866678 0.0 46-47 5.664111397211671E-5 0.0 0.0 0.07295375479608632 0.0 48-49 5.664111397211671E-5 0.0 0.0 0.08408373369160727 0.0 50-51 5.664111397211671E-5 0.0 0.0 0.09580844428383542 0.0 52-53 5.664111397211671E-5 0.0 0.0 0.12155183058416247 0.0 54-55 5.664111397211671E-5 0.0 0.0 0.1621351887451841 0.0 56-57 5.664111397211671E-5 0.0 0.0 0.2287734593333794 0.0 58-59 5.664111397211671E-5 0.0 0.0 0.3238455691355773 0.0 60-61 5.664111397211671E-5 0.0 0.0 0.44267862624907817 0.0 62-63 5.664111397211671E-5 0.0 0.0 0.513961468182987 0.0 64-65 5.664111397211671E-5 0.0 0.0 0.6087786929723105 0.0 66-67 5.664111397211671E-5 0.0 0.0 0.7854140068943565 0.0 68-69 5.664111397211671E-5 0.0 0.0 1.010505793819548 0.0 70-71 5.664111397211671E-5 0.0 0.0 1.2381747514304713 0.0 72-73 5.664111397211671E-5 0.0 0.0 1.5147249903993312 0.0 74-75 5.664111397211671E-5 0.0 0.0 1.797420790234166 0.0 76-77 5.664111397211671E-5 0.0 0.0 2.0646818865115986 0.0 78-79 5.664111397211671E-5 0.0 0.0 2.3398444181881413 0.0 80-81 5.664111397211671E-5 0.0 0.0 2.599912092991115 0.0 82-83 5.664111397211671E-5 0.0 0.0 2.8610842695165455 0.0 84-85 5.664111397211671E-5 0.0 0.0 3.305858616982592 0.0 86-87 5.664111397211671E-5 0.0 0.0 3.8999672614361245 0.0 88-89 5.664111397211671E-5 0.0 0.0 4.634998997452282 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 15310 0.0 29.240873 1 CGTATAG 95 0.0012156286 25.039309 1 GTACATG 8815 0.0 24.39451 1 TATCAAC 18380 0.0 24.291212 2 TCAACGC 18235 0.0 24.277145 4 CAACGCA 18300 0.0 24.240818 5 GGTATCA 10555 0.0 24.204271 1 ATCAACG 18340 0.0 24.188637 3 AGTACTT 10480 0.0 23.954847 12-13 GAGTACT 10165 0.0 23.785927 12-13 AACGCAG 18700 0.0 23.702093 6 TACATGG 8960 0.0 23.508493 2 CATTCCG 340 0.0 22.355038 9 ACATGGG 9100 0.0 22.10182 3 GTACTTT 10985 0.0 22.096544 14-15 TAGCCCT 1930 0.0 21.68099 4 ACGCAGA 20270 0.0 21.678772 7 CGCAGAG 20245 0.0 21.657997 8 AGAGTAC 15945 0.0 21.49543 10-11 CATGGGG 3470 0.0 20.554909 4 >>END_MODULE