FastQCFastQC Report
Thu 26 May 2016
SRR937474_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937474_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1765502
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139830.7920126966721079No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109350.6193705812850963No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76230.4317752118094457No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT53480.3029166775228802No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26660.15100520984966317No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT21730.12308114066140961No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT21130.1196826738230826No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTATC753.0685053E-431.6853031
GTATCAA186400.024.197441
AGCGTGC1000.001652968323.7525358
CTGGTCG2801.8189894E-1223.7525359
GAGTACT114400.023.08812912-13
GGTATCA138600.022.7695271
GTACATG95650.022.7080761
CTTTGCG1703.1484287E-622.3546942
TACATGG94500.022.2689422
AGTACTT118000.022.24284212-13
GTACTTT121550.021.72692714-15
CGTATCA1100.002876661521.5926022
ACATGGG97900.020.816243
ATCAACG218200.020.5515653
TCAACGC218200.020.5080224
TATCAAC219400.020.5041142
CAACGCA219300.020.3834955
AACGCAG223950.019.9586
AGAGTAC181600.019.59322510-11
ATAGCCC8150.019.2346383
Produced by FastQC (version 0.11.5)