FastQCFastQC Report
Thu 26 May 2016
SRR937473_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937473_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1941966
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137270.706860985207774No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99530.5125218464174965No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68910.35484658330784374No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT39850.20520441655518168No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT30670.15793273414673584No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN29720.1530407844421581No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT23870.12291667310344259No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23250.11972403224361293No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA174000.026.9209251
GTACATG97750.026.806361
TACATGG98850.026.0168862
CATTCCG4650.025.538579
ACATGGG99250.025.0971413
GGTATCA125350.023.5597291
ATGGGCG3050.023.3772015
GAGTACT107500.023.24271412-13
TCAACGC204500.022.7574774
TATCAAC205650.022.6995622
AGTACTT111200.022.68293612-13
ATCAACG205250.022.6274133
CAACGCA206000.022.5438695
AACGCAG209300.022.1287126
GTACTTT114400.021.59058814-15
CATGGGG42600.021.4252744
GTGTAGC15400.021.3074461
ACGCAGA227450.020.2584657
AGAGTAC172450.020.17687810-11
CGCAGAG228400.020.0494148
Produced by FastQC (version 0.11.5)