##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937473_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1941966 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.812646050445785 33.0 31.0 34.0 30.0 34.0 2 31.913273970811023 33.0 31.0 34.0 30.0 34.0 3 31.956392130449245 33.0 31.0 34.0 30.0 34.0 4 35.58563950141249 37.0 35.0 37.0 33.0 37.0 5 35.58521930867997 37.0 35.0 37.0 33.0 37.0 6 35.66580465363451 37.0 35.0 37.0 33.0 37.0 7 35.61844800578383 37.0 35.0 37.0 33.0 37.0 8 35.61421003251344 37.0 35.0 37.0 33.0 37.0 9 37.24214430118756 39.0 37.0 39.0 34.0 39.0 10-11 37.167259879936104 39.0 37.0 39.0 34.0 39.0 12-13 37.08606484356575 39.0 37.0 39.0 33.0 39.0 14-15 38.24796520639393 40.0 38.0 41.0 33.5 41.0 16-17 38.20503294084449 40.0 38.0 41.0 33.0 41.0 18-19 38.21846546232015 40.0 38.0 41.0 33.5 41.0 20-21 38.18197898418407 40.0 38.0 41.0 34.0 41.0 22-23 38.051875007080454 40.0 38.0 41.0 33.0 41.0 24-25 37.92494024097229 40.0 38.0 41.0 33.0 41.0 26-27 37.76850470090619 40.0 37.0 41.0 33.0 41.0 28-29 37.58924332351853 40.0 37.0 41.0 32.5 41.0 30-31 37.55483360676757 40.0 37.0 41.0 33.0 41.0 32-33 37.363474952702575 40.0 36.5 41.0 32.5 41.0 34-35 37.162856095317835 39.0 36.0 41.0 32.0 41.0 36-37 37.12592599458487 40.0 36.0 41.0 32.0 41.0 38-39 37.0726719726298 40.0 36.0 41.0 31.5 41.0 40-41 36.90156135586308 40.0 36.0 41.0 31.0 41.0 42-43 36.63278476554172 39.5 36.0 41.0 30.5 41.0 44-45 36.31359431627536 39.0 36.0 41.0 30.0 41.0 46-47 35.88743700971078 39.0 35.0 41.0 28.5 41.0 48-49 35.51133747964691 39.0 35.0 41.0 27.0 41.0 50-51 34.577221485855056 38.0 34.0 40.0 25.0 40.5 52-53 34.695487717086706 38.5 35.0 40.0 25.5 40.5 54-55 35.0581678566978 39.0 35.0 40.5 25.5 41.0 56-57 34.86792379475233 39.0 35.0 40.5 23.5 41.0 58-59 34.66924935863965 39.0 35.0 40.0 23.0 41.0 60-61 34.430470203906765 38.5 34.0 40.0 22.5 41.0 62-63 34.146223466322276 38.0 34.0 40.0 22.0 41.0 64-65 33.84714716941491 38.0 34.0 40.0 20.5 41.0 66-67 33.490018877776436 37.5 33.5 40.0 18.5 41.0 68-69 33.02262887197819 37.0 33.0 39.5 16.5 41.0 70-71 32.634988975090195 36.0 32.5 39.0 13.0 41.0 72-73 32.23481075363832 36.0 32.0 39.0 9.0 40.0 74-75 31.782120541760257 35.0 32.0 38.0 7.5 40.0 76-77 31.348550386567013 35.0 31.0 37.5 4.0 39.5 78-79 30.870488206281678 35.0 31.0 37.0 2.0 39.0 80-81 30.410206718346252 35.0 31.0 36.5 2.0 39.0 82-83 29.950175749729915 34.5 30.0 36.0 2.0 37.5 84-85 29.467900055922712 34.0 29.5 35.5 2.0 37.0 86-87 29.038450982149016 34.0 29.0 35.0 2.0 37.0 88-89 28.649262911915038 34.0 29.0 35.0 2.0 36.0 90-91 28.219703640537475 34.0 29.0 35.0 2.0 36.0 92-93 27.816966156977003 34.0 27.5 35.0 2.0 35.5 94-95 27.320307101154192 34.0 26.5 35.0 2.0 35.0 96-97 26.764394175799165 33.0 25.5 35.0 2.0 35.0 98-99 26.13467331559873 33.0 25.0 35.0 2.0 35.0 100-101 24.34470608651233 31.0 12.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.07873744867955779 1101 2 0.08252972323985475 1101 3 0.20478759222556775 1101 4 0.14074302876568368 1101 5 0.11734048324525759 1101 6 0.12673948965601056 1101 7 0.17335224319170095 1101 8 0.13379214974875708 1101 9 0.19425366959553259 1101 10-11 0.15988143992088766 1101 12-13 0.22135985633558874 1101 14-15 0.2727715686552159 1101 16-17 0.24360190152695793 1101 18-19 0.2380144088452596 1101 20-21 0.3040283468650813 1101 22-23 0.2543000512666893 1101 24-25 0.18745095174668336 1101 26-27 0.31551932832722684 1101 28-29 0.41406031061588777 1101 30-31 0.24539008621891867 1101 32-33 0.309139380153475 1101 34-35 0.2101513443955767 1101 36-37 0.31444712776096395 1101 38-39 0.46739716385744856 1101 40-41 0.48897709220539554 1101 42-43 0.4753317201679721 1101 44-45 0.4177718459126467 1101 46-47 0.4566225637809893 1101 48-49 0.44240591832377163 1101 50-51 0.4839761332203949 1101 52-53 0.6120558345130576 1101 54-55 0.5792739251044168 1101 56-57 0.6866210788663878 1101 58-59 0.5742154290769363 1101 60-61 0.6603671301202141 1101 62-63 0.6592425418338976 1101 64-65 0.6306206331332973 1101 66-67 0.7069619262912425 1101 68-69 0.6093046763360022 1101 70-71 0.6428730626415771 1101 72-73 0.6309719595474306 1101 74-75 0.5044541214346374 1101 76-77 0.6036052246178336 1101 78-79 0.747339297397037 1101 80-81 0.8442566915641869 1101 82-83 0.7652551837042516 1101 84-85 0.8579745256647939 1101 86-87 0.9958030158037481 1101 88-89 0.995644937066416 1101 90-91 1.0283588394615037 1101 92-93 1.120350009338292 1101 94-95 0.9112089086112434 1101 96-97 1.0422940695522378 1101 98-99 1.3186136622080085 1101 100-101 1.592736169539279 1102 1 0.19070465683243398 1102 2 0.017050835690664456 1102 3 0.21319177925311905 1102 4 0.04794979927832088 1102 5 0.19127054013451783 1102 6 0.16213230470516748 1102 7 0.09724089691805204 1102 8 0.10647780781057747 1102 9 0.29853091222168615 1102 10-11 0.041680266176229 1102 12-13 0.22753362583946313 1102 14-15 0.359530394440732 1102 16-17 0.3801043800698096 1102 18-19 0.3797860214995836 1102 20-21 0.3370971185802887 1102 22-23 0.4513841889086052 1102 24-25 0.3612259895922989 1102 26-27 0.39554432382099236 1102 28-29 0.4094285999944063 1102 30-31 0.4218610730598016 1102 32-33 0.2934597109812387 1102 34-35 0.4138839310113127 1102 36-37 0.4769255170265865 1102 38-39 0.6165858832235074 1102 40-41 0.5862161359527036 1102 42-43 0.4187362166463231 1102 44-45 0.4046685241276293 1102 46-47 0.5230488903198065 1102 48-49 0.624763585633886 1102 50-51 0.605756094270248 1102 52-53 0.5775642667087695 1102 54-55 0.6122585578224502 1102 56-57 0.5909013934129703 1102 58-59 0.6572280930689232 1102 60-61 0.7430486591595766 1102 62-63 0.6327423717488827 1102 64-65 0.6514450914023868 1102 66-67 0.7507380347914747 1102 68-69 0.7900332093720266 1102 70-71 0.5156411204515052 1102 72-73 0.38445090943853444 1102 74-75 0.5073911935963089 1102 76-77 0.6046426753936949 1102 78-79 0.5350928607117993 1102 80-81 0.5470086353444295 1102 82-83 0.6065557083250219 1102 84-85 0.5160832210518116 1102 86-87 0.5472820777296015 1102 88-89 0.7264064706594446 1102 90-91 0.8256118649643156 1102 92-93 0.6665731509280768 1102 94-95 0.4816854267159236 1102 96-97 0.7362781651359462 1102 98-99 0.8187084174007957 1102 100-101 1.0168031527643357 1103 1 0.08843733541119292 1103 2 0.07159291676909518 1103 3 0.11536557108548706 1103 4 0.012579623069242984 1103 5 -0.10996330542872101 1103 6 -0.050606700526273585 1103 7 0.08274120073499347 1103 8 0.09683965722889099 1103 9 0.11046929278366235 1103 10-11 0.09977051629789457 1103 12-13 0.22959165641761814 1103 14-15 0.254220395577228 1103 16-17 0.25280626767344216 1103 18-19 0.2707527525360618 1103 20-21 0.2951160470519767 1103 22-23 0.28036443404533884 1103 24-25 0.26400848299812907 1103 26-27 0.14618962341344144 1103 28-29 0.18452137626911735 1103 30-31 0.10073890305945099 1103 32-33 0.10206286721337676 1103 34-35 0.22629482343591434 1103 36-37 0.1605326177777897 1103 38-39 0.22270008944747843 1103 40-41 0.2675464484369101 1103 42-43 0.1655556293270095 1103 44-45 0.13097525135363952 1103 46-47 0.22281428337541342 1103 48-49 0.2227313219592162 1103 50-51 0.06659384131280888 1103 52-53 0.1669976059974374 1103 54-55 0.268830221488912 1103 56-57 0.30956583738763044 1103 58-59 0.39228228067712934 1103 60-61 0.41988340623226605 1103 62-63 0.26079415420883123 1103 64-65 0.38943839058389784 1103 66-67 0.5353588710105299 1103 68-69 0.6165838680295934 1103 70-71 0.5113225542112261 1103 72-73 0.5756286396210939 1103 74-75 0.5459569640919675 1103 76-77 0.6072199589024727 1103 78-79 0.6763709418319905 1103 80-81 0.6232378601853377 1103 82-83 0.7319213029603766 1103 84-85 0.6308095283721968 1103 86-87 0.6531326833111493 1103 88-89 0.7266971194325897 1103 90-91 0.8439058919066227 1103 92-93 0.8080901572030008 1103 94-95 0.8716880342506315 1103 96-97 1.1593053539644025 1103 98-99 1.078443787031297 1103 100-101 1.0934825866232316 1104 1 0.06301486482045604 1104 2 0.08278952974359655 1104 3 0.15537168014980907 1104 4 -0.033999488687825874 1104 5 0.10437947567273653 1104 6 0.011963888585320603 1104 7 0.060737803193887885 1104 8 0.14855964676336697 1104 9 0.19994605392800935 1104 10-11 0.17265891445865833 1104 12-13 0.1492751117444513 1104 14-15 0.23062964279779408 1104 16-17 0.13852983501648453 1104 18-19 0.09279020059961596 1104 20-21 0.1479699690587566 1104 22-23 0.23965828629862074 1104 24-25 0.3629022165753213 1104 26-27 0.414761241551858 1104 28-29 0.19693679765059358 1104 30-31 0.13542424243174622 1104 32-33 -0.005736329845255739 1104 34-35 0.15162277878967245 1104 36-37 0.10362130061094632 1104 38-39 -0.03519255876931737 1104 40-41 -0.015383724206749605 1104 42-43 0.06035316016338754 1104 44-45 0.05712943262009418 1104 46-47 0.14578636820628077 1104 48-49 -0.0011189408682525936 1104 50-51 -0.032118998863801096 1104 52-53 -0.036631904288448425 1104 54-55 -0.07053944325114969 1104 56-57 -0.007692018956724667 1104 58-59 -0.1424318514163616 1104 60-61 -0.015633542039729775 1104 62-63 0.09560317709654242 1104 64-65 0.12591551351504648 1104 66-67 0.15830529276380645 1104 68-69 0.015236368677435053 1104 70-71 0.049429720719530224 1104 72-73 0.161336308374608 1104 74-75 0.046606177362161105 1104 76-77 0.11228109336072478 1104 78-79 0.07898444461675069 1104 80-81 0.1809094303776142 1104 82-83 0.3431072751466111 1104 84-85 0.3031847901668847 1104 86-87 0.061688753380114036 1104 88-89 0.06820007444208542 1104 90-91 0.25252918672030233 1104 92-93 0.23605853027655144 1104 94-95 0.4873564071989911 1104 96-97 0.5842170667182032 1104 98-99 0.5935179032512359 1104 100-101 0.6130910556892069 1105 1 0.13456428726501812 1105 2 0.2725003304518374 1105 3 0.18251256995054987 1105 4 0.19507923310189312 1105 5 0.19527489738657522 1105 6 0.06924586492100104 1105 7 0.1272859609185204 1105 8 0.19321269192791846 1105 9 0.2837506125407572 1105 10-11 0.3794645129522394 1105 12-13 0.28021789019362586 1105 14-15 0.5040346904355815 1105 16-17 0.46300216575480135 1105 18-19 0.42797400656347406 1105 20-21 0.45678225651554527 1105 22-23 0.3284697306087807 1105 24-25 0.35380747499976906 1105 26-27 0.372621475946616 1105 28-29 0.23672348155492529 1105 30-31 0.41899623310092693 1105 32-33 0.38887807109906447 1105 34-35 0.512649731376726 1105 36-37 0.5684381972692734 1105 38-39 0.4643823548081585 1105 40-41 0.6177203200865549 1105 42-43 0.6412980464791218 1105 44-45 0.7728758984304918 1105 46-47 0.710970033645701 1105 48-49 0.9236780013199919 1105 50-51 0.7630483657409073 1105 52-53 0.7913992078219536 1105 54-55 0.931100476930979 1105 56-57 1.0117460238189224 1105 58-59 0.9958933783410941 1105 60-61 1.1439396852444972 1105 62-63 1.0536264198384586 1105 64-65 1.0200999096815409 1105 66-67 0.9654558787507099 1105 68-69 1.138652658270189 1105 70-71 1.1523870575003983 1105 72-73 1.1941073097517432 1105 74-75 1.0192713638380901 1105 76-77 1.2443951429939553 1105 78-79 1.2604050061523964 1105 80-81 1.1887647464170712 1105 82-83 1.3966140624769245 1105 84-85 1.2882744447578176 1105 86-87 1.2774998604316288 1105 88-89 1.4818815336478401 1105 90-91 1.5417671226973724 1105 92-93 1.4875340368671495 1105 94-95 1.6640729131993872 1105 96-97 1.5111387383969053 1105 98-99 1.5567953547000464 1105 100-101 1.8787792038581728 1106 1 0.04755998721724097 1106 2 0.14713004855981993 1106 3 -0.02274304400071614 1106 4 0.06815615617881576 1106 5 0.06075506938848463 1106 6 0.07571982574279446 1106 7 0.14049666325965404 1106 8 0.010890666127629345 1106 9 0.055919747753371496 1106 10-11 -0.021897168844226655 1106 12-13 -0.05909171324640994 1106 14-15 0.018499573378726097 1106 16-17 -0.029592196404777837 1106 18-19 -0.03572642344129662 1106 20-21 0.03291699134592818 1106 22-23 0.17866084384337455 1106 24-25 -0.08828521491901142 1106 26-27 0.23653690834031238 1106 28-29 0.21739715570685547 1106 30-31 0.3314783162351773 1106 32-33 0.3225976124273018 1106 34-35 0.37129760524199185 1106 36-37 0.19925520634715355 1106 38-39 0.2718954938430258 1106 40-41 0.3923619827716962 1106 42-43 0.33781022994782006 1106 44-45 0.2983059032083233 1106 46-47 0.41856678472071707 1106 48-49 0.42527857465969987 1106 50-51 0.3398758860466842 1106 52-53 0.25949524222232867 1106 54-55 0.47125814534950905 1106 56-57 0.4689247146932658 1106 58-59 0.47221922640606806 1106 60-61 0.33000036369647745 1106 62-63 0.49107266924122683 1106 64-65 0.6505227477130759 1106 66-67 0.6615022237100945 1106 68-69 0.5713927442711508 1106 70-71 0.5072485004053178 1106 72-73 0.650521547687724 1106 74-75 0.5369971163464449 1106 76-77 0.5278638482017932 1106 78-79 0.615959234532717 1106 80-81 0.5375225103922254 1106 82-83 0.540056489615008 1106 84-85 0.5281573879016292 1106 86-87 0.4419408542204515 1106 88-89 0.5846048972407694 1106 90-91 0.6857590003848983 1106 92-93 0.5422879785776118 1106 94-95 0.7120184459275265 1106 96-97 0.6304172859363248 1106 98-99 0.8177963102662176 1106 100-101 1.1621045741400664 1107 1 0.15513650783723776 1107 2 -0.03569687774536945 1107 3 0.10054048797846704 1107 4 0.08861947664213687 1107 5 0.11436706647874217 1107 6 0.09309993877507594 1107 7 -0.007072023439462782 1107 8 0.04738554610077017 1107 9 0.10908968787983042 1107 10-11 0.14228502577275037 1107 12-13 0.2506391356148683 1107 14-15 0.30201756341845254 1107 16-17 0.3935486463012836 1107 18-19 0.36943277302224686 1107 20-21 0.3997878995211863 1107 22-23 0.26908947122852567 1107 24-25 0.3552825764748633 1107 26-27 0.4161938945190329 1107 28-29 0.289010383841827 1107 30-31 0.18050746961215935 1107 32-33 0.14336030058129978 1107 34-35 0.10947677820377066 1107 36-37 0.1570779359090153 1107 38-39 0.17853194395774352 1107 40-41 0.16953449690073086 1107 42-43 0.16964672482779974 1107 44-45 0.07881342936802582 1107 46-47 -0.04855872588306198 1107 48-49 0.012436540078532232 1107 50-51 0.07094857364111107 1107 52-53 0.22505676647951134 1107 54-55 0.1494154952459894 1107 56-57 0.1708664079393074 1107 58-59 0.19534887779659016 1107 60-61 0.2101385764433914 1107 62-63 0.3305024717145102 1107 64-65 0.4110537756354091 1107 66-67 0.31706710536193583 1107 68-69 0.3250327196502525 1107 70-71 0.33654456665790633 1107 72-73 0.27723521787964955 1107 74-75 0.2610444556111773 1107 76-77 0.19449414309295676 1107 78-79 0.29941847016586465 1107 80-81 0.25607491372723956 1107 82-83 0.347467938330805 1107 84-85 0.3442169257003016 1107 86-87 0.39458480251657235 1107 88-89 0.40553265729896637 1107 90-91 0.5653712213014686 1107 92-93 0.5665955159286256 1107 94-95 0.5787298778563503 1107 96-97 0.3791666564248253 1107 98-99 0.6474868703915604 1107 100-101 1.1504404255193954 1108 1 -0.056827094034620274 1108 2 -0.15518727246512043 1108 3 -0.06414647946079555 1108 4 -0.06146376518422869 1108 5 -0.014297640167619363 1108 6 -0.01182805606117654 1108 7 0.00223807302659651 1108 8 0.057681556396779854 1108 9 0.0964361431161933 1108 10-11 0.06925459035699078 1108 12-13 0.1420764444833722 1108 14-15 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-2.3356068250506787 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6643.0 3 1024.0 4 803.0 5 984.0 6 1103.0 7 1331.0 8 1742.0 9 2279.0 10 2890.0 11 4482.0 12 6690.0 13 9302.0 14 11098.0 15 14583.0 16 20094.0 17 27964.0 18 22320.0 19 12728.0 20 11508.0 21 11010.0 22 11661.0 23 13213.0 24 14768.0 25 16709.0 26 19158.0 27 22608.0 28 27070.0 29 32709.0 30 40416.0 31 50112.0 32 64291.0 33 84617.0 34 115997.0 35 164274.0 36 244014.0 37 381629.0 38 400829.0 39 67302.0 40 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.94411144640569 20.697108428754497 15.726148154272249 21.632631970567566 2 14.226573770432532 21.493677646486407 41.804028476287236 22.475720106793826 3 17.855737176540348 26.67077130321866 31.805578655614454 23.66791286462654 4 11.06258022360595 18.11998481401037 40.91126268404619 29.906172278337493 5 11.65842324320053 38.874270447985346 35.922751280670106 13.544555028144014 6 27.837882470726054 39.146733549049664 19.230491348123834 13.784892632100448 7 25.42198192283892 34.67097925611445 22.702046828445543 17.20499199260109 8 24.617224300083144 34.66226402143245 21.676043275704306 19.044468402780108 9 25.67917315851322 16.73319787974012 22.67770800960114 34.90992095214553 10-11 22.89534363092788 27.63922204172113 29.433615095683503 20.03181923166749 12-13 24.587761396706735 25.860225584457915 29.337153719444203 20.21485929939115 14-15 21.548020047862014 27.041882486502526 28.954221468241425 22.45587599739404 16-17 19.454247522708506 30.081311932287363 29.497109826589597 20.967330718414534 18-19 19.08407542932513 28.491532184613288 32.9481073778541 19.476285008207476 20-21 20.736261887510658 27.163981074938537 32.68012662121184 19.41963041633897 22-23 19.913577185656816 27.544272534012183 32.433661188198876 20.108489092132128 24-25 19.67318811227241 27.638863446966276 32.88379821101 19.80415022975131 26-27 19.588050768056906 27.683180365322137 32.995056805324005 19.733712061296956 28-29 19.19973803865994 28.085986900384764 32.905693659539544 19.808581401415758 30-31 19.72469417058319 27.632156191581185 32.90054595621822 19.74260368161741 32-33 19.306647714266763 27.729497846564527 32.84370386255794 20.12015057661077 34-35 19.87266655554105 27.87678164383689 32.56867382584001 19.681877974782054 36-37 19.761407148238742 27.586824369652497 32.58687597403267 20.064892508076085 38-39 19.460564095035952 28.272140006485984 32.471029133016636 19.796266765461425 40-41 19.838141078564117 27.97056116046366 32.508524322182225 19.68277343879 42-43 19.45317478541077 28.126552821071154 31.917977507797968 20.50229488572011 44-45 19.981652891282415 28.080211185285155 31.604455947585585 20.333679975846845 46-47 20.102742597798336 28.320860333797338 31.035153604311986 20.54124346409234 48-49 20.024843784512424 28.41981351163333 30.944781153816773 20.610561550037467 50-51 19.95737831749644 28.78333883437595 30.669366349567472 20.589916498560136 52-53 20.15592206529246 29.116859734663556 30.158250030712626 20.568968169331352 54-55 20.310699535906892 28.575985407506682 30.277617127185465 20.83569792940097 56-57 20.57407750902865 28.955302660727284 29.664463604324876 20.80615622591919 58-59 20.573206922746554 28.76656817440224 29.894745259280565 20.76547964357064 60-61 20.559942895926692 28.81338530209876 30.16021696245851 20.46645483951604 62-63 20.3399882725303 28.799167468318938 30.264636828949982 20.59620743020079 64-65 20.245366487750378 28.895156877863904 30.087086581226764 20.772390053158958 66-67 20.377184414169104 28.78924618328981 29.739689193603063 21.093880208938018 68-69 20.43335211348993 28.665690511274285 30.033391531181252 20.867565844054532 70-71 20.385087687616412 28.5737282844804 30.19420781887696 20.846976209026227 72-73 20.370911260167343 28.239074589926215 30.376525810936194 21.013488338970244 74-75 20.12161803063412 28.827910744645997 29.99055744607296 21.05991377864693 76-77 19.905730285822802 28.5720883621179 30.20723030336807 21.314951048691224 78-79 19.86827483482752 28.410698496335073 30.232052917116143 21.488973751721257 80-81 19.921606949192594 28.59654046936769 30.03544042819508 21.446412153244637 82-83 19.999514929965436 28.64728158238102 30.152520983794272 21.200682503859277 84-85 20.01224305955761 28.445301693649032 29.940136443300915 21.602318803492444 86-87 19.718471184766024 28.59438103259909 30.03731617623348 21.649831606401406 88-89 20.135053097854286 28.115249988583464 29.961297300253847 21.788399613308403 90-91 19.880959383471666 27.886158348631994 30.32834591796615 21.90453634993019 92-93 19.899505007333943 27.863868631500733 30.61036194968026 21.626264411485067 94-95 19.644183533123304 27.840495853056268 30.544632889635594 21.970687724184835 96-97 19.44348416537072 27.79878065769772 30.58499742530548 22.17273775162608 98-99 19.49580174493846 27.920021589846577 30.239278866099607 22.344897799115355 100-101 19.355277236927503 27.655230907813316 30.845877792257774 22.143614063001404 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3149.0 1 1829.0 2 486.0 3 460.0 4 412.0 5 409.0 6 637.0 7 1226.0 8 1974.0 9 2356.0 10 2218.5 11 1999.0 12 2116.0 13 2288.5 14 2418.5 15 2676.0 16 2963.5 17 3284.0 18 3723.5 19 4282.0 20 4917.5 21 5754.5 22 6818.0 23 8013.0 24 9527.0 25 12091.0 26 16475.5 27 21381.0 28 24883.0 29 29335.0 30 35801.0 31 41968.0 32 49465.0 33 58087.5 34 66473.5 35 73175.5 36 78859.5 37 82692.0 38 81104.0 39 77859.5 40 75826.5 41 74273.5 42 72010.5 43 71232.5 44 70978.5 45 69039.0 46 66930.5 47 67094.5 48 74060.5 49 71055.0 50 57775.0 51 49644.5 52 43671.5 53 39108.5 54 34923.0 55 30965.0 56 26921.5 57 23128.5 58 19655.5 59 16311.0 60 13663.5 61 11417.0 62 9209.0 63 6874.0 64 5032.0 65 3868.5 66 2978.0 67 2330.0 68 1786.0 69 1347.0 70 1099.5 71 887.0 72 678.5 73 567.5 74 464.5 75 365.5 76 270.0 77 164.0 78 99.0 79 72.0 80 54.5 81 36.0 82 19.5 83 9.5 84 7.0 85 9.0 86 7.5 87 6.5 88 5.5 89 5.0 90 3.5 91 2.0 92 2.0 93 1.0 94 1.5 95 2.0 96 1.0 97 0.5 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3442902707874391 2 0.30376432955056887 3 0.3060815688843162 4 0.3074719124845646 5 0.2922296270892487 6 0.21812946261675023 7 0.22569911110699156 8 0.22363934281032727 9 0.22034371353566437 10-11 0.22958692376694548 12-13 0.22994738321886174 14-15 0.2113064801340497 16-17 0.22482370958090925 18-19 0.21107475620067498 20-21 0.2172025668832513 22-23 0.21460210940871263 24-25 0.20602832387384742 26-27 0.21892762283170766 28-29 0.22016348380970624 30-31 0.22904623458907106 32-33 0.2307197963301108 34-35 0.21725406109066792 36-37 0.21349498394925553 38-39 0.20296441853255928 40-41 0.2050499339329319 42-43 0.20396855557718313 44-45 0.22302141232132797 46-47 0.2118214222082158 48-49 0.2192623351799156 50-51 0.229380946937279 52-53 0.23955105290205903 54-55 0.23334600090835783 56-57 0.19724856150931583 58-59 0.18401455020324764 60-61 0.1931290249159872 62-63 0.19472534534590205 64-65 0.1916871871083222 66-67 0.20468947448101563 68-69 0.1931032778122789 70-71 0.19753177965010715 72-73 0.2133919955344223 74-75 0.20242372935468492 76-77 0.21225912297125699 78-79 0.20945268866705186 80-81 0.19485408086444356 82-83 0.2112034917192165 84-85 0.1918159226268637 86-87 0.1956007468719844 88-89 0.2053331520737232 90-91 0.20020947843577075 92-93 0.20898924080030237 94-95 0.2045607389624741 96-97 0.19938557111710506 98-99 0.20690372539992974 100-101 0.1884687991447842 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1941966.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.40662809694032 #Duplication Level Percentage of deduplicated Percentage of total 1 75.81799667665514 42.008195449184875 2 13.952586109156584 15.461314990811486 3 4.710882811836843 7.830423958911371 4 2.0646621853575153 4.5758387939967955 5 1.0786572707959636 2.98823811235263 6 0.6103366923626029 2.029001887659084 7 0.36820182236785187 1.4280575005585872 8 0.24640240636620916 1.0921861193378968 9 0.17155827466769202 0.8554918969318991 >10 0.8332310387569362 8.555763375190102 >50 0.08988886966156458 3.439218982125627 >100 0.05029769907164918 5.33113138370299 >500 0.0034391454194591152 1.2778277318516074 >1k 0.0015801478954271596 1.5528913267177773 >5k 1.8589975240319525E-4 0.8674726066140762 >10k+ 9.294987620159763E-5 0.70694588405316 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13727 0.706860985207774 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9953 0.5125218464174965 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6891 0.35484658330784374 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3985 0.20520441655518168 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3067 0.15793273414673584 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2972 0.1530407844421581 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2387 0.12291667310344259 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2325 0.11972403224361293 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.5448262224982312E-4 0.0 22-23 0.0 0.0 0.0 5.406891778743809E-4 0.0 24-25 0.0 0.0 0.0 8.239073186657233E-4 0.0 26-27 0.0 0.0 0.0 0.001416090703956712 0.0 28-29 0.0 0.0 0.0 0.0032183879635379817 0.0 30-31 0.0 0.0 0.0 0.00674574117157561 0.0 32-33 0.0 0.0 0.0 0.010685048038946099 0.0 34-35 0.0 0.0 0.0 0.015010561461941147 0.0 36-37 0.0 0.0 0.0 0.02010848799618531 0.0 38-39 0.0 0.0 0.0 0.030149858442423814 0.0 40-41 0.0 0.0 0.0 0.04016548178495401 0.0 42-43 0.0 0.0 0.0 0.051648690038857525 0.0 44-45 0.0 0.0 0.0 0.06104638289238844 0.0 46-47 0.0 0.0 0.0 0.07603119725062127 0.0 48-49 0.0 0.0 0.0 0.08844130123802374 0.0 50-51 0.0 0.0 0.0 0.09940956741776118 0.0 52-53 0.0 0.0 0.0 0.12479621167414877 0.0 54-55 0.0 0.0 0.0 0.16650651968160102 0.0 56-57 0.0 0.0 0.0 0.2319556573081094 0.0 58-59 0.0 0.0 0.0 0.3254433908729607 2.5747103708303853E-5 60-61 0.0 0.0 0.0 0.44568236519073967 5.1494207416607705E-5 62-63 0.0 0.0 0.0 0.5187526455149061 5.1494207416607705E-5 64-65 0.0 0.0 0.0 0.6158449735989198 5.1494207416607705E-5 66-67 0.0 0.0 0.0 0.7897924062522207 5.1494207416607705E-5 68-69 0.0 0.0 0.0 1.0087200290839284 5.1494207416607705E-5 70-71 0.0 0.0 0.0 1.2317414414052563 5.1494207416607705E-5 72-73 0.0 0.0 0.0 1.4904483394662935 5.1494207416607705E-5 74-75 0.0 0.0 0.0 1.761565341514733 5.1494207416607705E-5 76-77 0.0 0.0 0.0 2.022615226013226 5.1494207416607705E-5 78-79 0.0 0.0 0.0 2.2879133826235885 5.1494207416607705E-5 80-81 0.0 0.0 0.0 2.5293697212000623 5.1494207416607705E-5 82-83 0.0 0.0 0.0 2.776438928384946 5.1494207416607705E-5 84-85 0.0 0.0 0.0 3.1979705102972966 5.1494207416607705E-5 86-87 0.0 0.0 0.0 3.7624500119981503 5.1494207416607705E-5 88-89 0.0 0.0 0.0 4.457930777366854 5.1494207416607705E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 17400 0.0 26.920925 1 GTACATG 9775 0.0 26.80636 1 TACATGG 9885 0.0 26.016886 2 CATTCCG 465 0.0 25.53857 9 ACATGGG 9925 0.0 25.097141 3 GGTATCA 12535 0.0 23.559729 1 ATGGGCG 305 0.0 23.377201 5 GAGTACT 10750 0.0 23.242714 12-13 TCAACGC 20450 0.0 22.757477 4 TATCAAC 20565 0.0 22.699562 2 AGTACTT 11120 0.0 22.682936 12-13 ATCAACG 20525 0.0 22.627413 3 CAACGCA 20600 0.0 22.543869 5 AACGCAG 20930 0.0 22.128712 6 GTACTTT 11440 0.0 21.590588 14-15 CATGGGG 4260 0.0 21.425274 4 GTGTAGC 1540 0.0 21.307446 1 ACGCAGA 22745 0.0 20.258465 7 AGAGTAC 17245 0.0 20.176878 10-11 CGCAGAG 22840 0.0 20.049414 8 >>END_MODULE