FastQCFastQC Report
Thu 26 May 2016
SRR937473_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937473_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1941966
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150980.7774595435759432No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120280.6193723268069575No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82010.4223039950235998No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT55510.28584434536958936No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27970.14402929814425175No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT23820.12265920206635955No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT21840.11246334899787122No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG105500.023.8369121
GAGTACT120050.022.9089512-13
TACATGG107900.022.8519972
ACATGGG108450.022.2104133
GTACTTT125900.021.69244814-15
GTATCAA208450.021.6882951
GGTATCA161000.021.6433681
AGTACTT123700.021.36900312-13
GTAACCG1150.003725540420.650886
CATGGGT25000.020.5244
CATGGGG41300.019.9009724
ATGGGCG2651.8524588E-819.7208255
TAGGACG6150.019.3127084
AGAGTAC196000.018.84226410-11
ACTTTTT148400.018.6757916-17
TGCGTAC2051.5911724E-518.5354236
ATCAACG244100.018.4314923
TCAACGC245100.018.3756754
TATCAAC245350.018.3375852
CAACGCA246350.018.3017185
Produced by FastQC (version 0.11.5)