FastQCFastQC Report
Thu 26 May 2016
SRR937472_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937472_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2015760
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136850.6789002659046712No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100790.5000099218160892No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70880.3516291622018494No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT43200.21431122752708656No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT31540.1564670397269516No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN30340.15051395007342144No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT25660.12729690042465372No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23780.11797039330078977No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA180450.024.917061
GTACATG100450.024.4153141
GTGTAGC14100.024.2703441
CATTCCG3950.024.0535629
TACATGG101750.023.8136232
GGAGCGT2002.0516381E-823.7546636
TAGGACG4100.023.177014
GAGTACT108650.023.01822912-13
ACATGGG102600.023.0149233
GGATCGA1252.2537216E-422.8044786
GGTATCA133700.022.7871061
TCAACGC207850.021.3962234
ATCAACG208350.021.3443433
CAACGCA208400.021.3386945
GTACTTT117000.021.31404714-15
TATCAAC210750.021.213472
AGTACTT112100.021.20809712-13
TAGCCCT21000.021.0414164
GGACGTG4550.020.883226
AACGCAG213350.020.843096
Produced by FastQC (version 0.11.5)