##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937472_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2015760 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.961773226971466 33.0 31.0 34.0 30.0 34.0 2 32.06626830575068 33.0 31.0 34.0 30.0 34.0 3 32.10758919712664 34.0 31.0 34.0 30.0 34.0 4 35.707644263205935 37.0 35.0 37.0 35.0 37.0 5 35.73968031908561 37.0 35.0 37.0 35.0 37.0 6 35.77641733142834 37.0 35.0 37.0 35.0 37.0 7 35.68425556613882 37.0 35.0 37.0 35.0 37.0 8 35.6991214231853 37.0 35.0 37.0 35.0 37.0 9 37.35551901019963 39.0 37.0 39.0 35.0 39.0 10-11 37.26381563281343 39.0 37.0 39.0 34.0 39.0 12-13 37.146741923641706 39.0 37.0 39.0 34.0 39.0 14-15 38.34954533277771 40.0 38.0 41.0 34.0 41.0 16-17 38.305522978926064 40.0 38.0 41.0 34.0 41.0 18-19 38.32478345636385 40.0 38.0 41.0 34.0 41.0 20-21 38.25275404810097 40.0 38.0 41.0 34.0 41.0 22-23 38.107528922093906 40.0 38.0 41.0 33.5 41.0 24-25 38.057031591062426 40.0 38.0 41.0 33.5 41.0 26-27 38.07629231654562 40.0 38.0 41.0 33.5 41.0 28-29 37.99651769059809 40.0 38.0 41.0 33.0 41.0 30-31 37.78113267452474 40.0 37.5 41.0 33.0 41.0 32-33 37.51560503234512 40.0 37.0 41.0 33.0 41.0 34-35 37.50200693534944 40.0 37.0 41.0 33.0 41.0 36-37 37.368522294320755 40.0 37.0 41.0 33.0 41.0 38-39 37.24856307298488 40.0 37.0 41.0 32.5 41.0 40-41 37.02944993451601 40.0 36.5 41.0 31.5 41.0 42-43 36.73598791522801 40.0 36.0 41.0 31.0 41.0 44-45 36.41670833829424 40.0 36.0 41.0 30.0 41.0 46-47 35.97830743739334 39.0 36.0 41.0 29.5 41.0 48-49 35.592875639957136 39.0 35.0 41.0 27.0 41.0 50-51 34.61263642497123 38.0 34.0 40.0 25.0 40.5 52-53 34.718012561019165 38.5 35.0 40.0 25.5 40.5 54-55 35.098309818629204 39.0 35.0 40.0 25.5 41.0 56-57 34.9181274060404 39.0 35.0 40.5 24.5 41.0 58-59 34.69835347461999 39.0 35.0 40.0 23.5 41.0 60-61 34.43489849982141 38.0 34.0 40.0 22.5 41.0 62-63 34.165741457316344 38.0 34.0 40.0 22.0 41.0 64-65 33.80972337976743 38.0 34.0 40.0 20.5 41.0 66-67 33.47983242052625 37.5 33.5 40.0 18.5 41.0 68-69 33.03387977735444 37.0 33.0 39.5 17.0 41.0 70-71 32.60138012461801 36.0 32.5 39.0 12.5 40.5 72-73 32.160449904750564 36.0 32.0 39.0 8.0 40.0 74-75 31.685683067428663 35.0 31.5 38.0 7.0 40.0 76-77 31.213663829027265 35.0 31.0 37.0 2.0 39.0 78-79 30.71467883081319 35.0 31.0 37.0 2.0 39.0 80-81 30.209366442433623 34.5 30.0 36.0 2.0 39.0 82-83 29.722715997936263 34.0 30.0 36.0 2.0 37.5 84-85 29.18929733698456 34.0 29.0 35.0 2.0 37.0 86-87 28.749805036313845 34.0 29.0 35.0 2.0 36.5 88-89 28.29830212922173 34.0 28.5 35.0 2.0 36.0 90-91 27.803722913442076 34.0 27.5 35.0 2.0 36.0 92-93 27.34519163987776 33.5 26.0 35.0 2.0 35.0 94-95 26.855385561773225 33.0 25.5 35.0 2.0 35.0 96-97 26.191107324284637 33.0 24.5 35.0 2.0 35.0 98-99 25.361860538953053 32.5 21.0 34.5 2.0 35.0 100-101 22.820152944795016 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.13111280676881165 1101 2 0.15239649214009177 1101 3 0.045953287229892226 1101 4 0.08122842482690373 1101 5 0.01059604459113217 1101 6 -0.0791263586234976 1101 7 0.059531069591216124 1101 8 -0.031179307936973544 1101 9 0.056721497748732475 1101 10-11 -0.05583500209444736 1101 12-13 0.020473770089502352 1101 14-15 -0.05737201956140581 1101 16-17 -0.11108245719789522 1101 18-19 0.013483490814671484 1101 20-21 0.1626725017047832 1101 22-23 0.01625434283872096 1101 24-25 0.06116164621195708 1101 26-27 0.008937266874845307 1101 28-29 0.02404668489086248 1101 30-31 0.012349838012433167 1101 32-33 -0.11934636223029571 1101 34-35 -0.09850648017452812 1101 36-37 0.04211294735583948 1101 38-39 0.0077947658671888576 1101 40-41 -0.006432618692016945 1101 42-43 0.09955669547991164 1101 44-45 0.1471493819107792 1101 46-47 0.15667708729918672 1101 48-49 0.1374579886462115 1101 50-51 0.13399273237038045 1101 52-53 0.12505276522179543 1101 54-55 0.09098006387481661 1101 56-57 0.10519543867145842 1101 58-59 0.23048305591081686 1101 60-61 0.22918274756530366 1101 62-63 0.024730395122148252 1101 64-65 -0.11842806771451819 1101 66-67 -0.0143143476353913 1101 68-69 -0.07554576746394304 1101 70-71 -0.07475760961058597 1101 72-73 0.10965011012886805 1101 74-75 0.12733024775328516 1101 76-77 0.19299388910889448 1101 78-79 0.09011726698195233 1101 80-81 0.2540621642090173 1101 82-83 0.33452610188473386 1101 84-85 0.2906555714837147 1101 86-87 0.27144803326749667 1101 88-89 0.3227385270543728 1101 90-91 0.39590741883710123 1101 92-93 0.4579529253187715 1101 94-95 0.23736333924489372 1101 96-97 0.4538644543761414 1101 98-99 0.32235320789524735 1101 100-101 0.5323028169257427 1102 1 -0.14441791640795287 1102 2 -0.11433421704062852 1102 3 -0.08133863159092414 1102 4 -0.20228799693546762 1102 5 -0.061071133673429756 1102 6 -0.11078234005116627 1102 7 -0.12068233830287767 1102 8 -0.15117896863888802 1102 9 -0.13813580354850075 1102 10-11 -0.20898594149951322 1102 12-13 -0.10387314736085784 1102 14-15 -0.10054080922681408 1102 16-17 -0.049990511127212756 1102 18-19 -0.09284563787313971 1102 20-21 -0.1422112171294927 1102 22-23 -0.1389413976979128 1102 24-25 -0.08225726976245795 1102 26-27 -0.14021578678143953 1102 28-29 -0.16454738576902628 1102 30-31 -0.1653132676147706 1102 32-33 -0.28534371040368 1102 34-35 -0.10804367228140421 1102 36-37 -0.13011476195264038 1102 38-39 -0.08436787242992239 1102 40-41 -0.19392131980062288 1102 42-43 -0.31647312458077437 1102 44-45 -0.13464695759300582 1102 46-47 -0.09669216921917467 1102 48-49 -0.08148205473092673 1102 50-51 -0.24279832415614777 1102 52-53 -0.3261612062252084 1102 54-55 -0.2017581088035243 1102 56-57 -0.28399404711699106 1102 58-59 -0.2720698971223712 1102 60-61 -0.08323126820251758 1102 62-63 -0.196912899737967 1102 64-65 -0.14934660595474014 1102 66-67 -0.2718347041952711 1102 68-69 -0.16701151929814273 1102 70-71 -0.16576159795358336 1102 72-73 -0.20761772382924448 1102 74-75 -0.31577847554753546 1102 76-77 -0.19553608602161532 1102 78-79 -0.2565923633050673 1102 80-81 -0.31881164287987573 1102 82-83 -0.24813725026373845 1102 84-85 -0.1640200356875603 1102 86-87 -0.03549480083641399 1102 88-89 -0.2401999428278856 1102 90-91 -0.2476154071766743 1102 92-93 -0.1444488280040943 1102 94-95 -0.13600690335854182 1102 96-97 0.019510151890834493 1102 98-99 -0.01922612927533507 1102 100-101 0.27312626157535647 1103 1 -0.03451758959070972 1103 2 -0.06768497625465386 1103 3 -0.10888556563703133 1103 4 -0.05077561605362746 1103 5 -0.04028699990501394 1103 6 -0.012721736024694508 1103 7 0.019107405349188866 1103 8 0.03154838422632622 1103 9 0.003758201034230524 1103 10-11 -0.10033594187565598 1103 12-13 -0.0752546971840502 1103 14-15 -0.06428566069307351 1103 16-17 -0.13083018241576383 1103 18-19 -0.06309828613827762 1103 20-21 -0.09697645479293016 1103 22-23 -0.09079193788284812 1103 24-25 -0.14041658058180673 1103 26-27 -0.13824462146375538 1103 28-29 -0.3372092382323473 1103 30-31 -0.2834498017239966 1103 32-33 -0.19567478477654277 1103 34-35 -0.17389070144265162 1103 36-37 -0.1484602561742676 1103 38-39 -0.3958450736336374 1103 40-41 -0.20619861853063526 1103 42-43 -0.0827066904070719 1103 44-45 -0.193635994179445 1103 46-47 -0.14241070517514487 1103 48-49 -0.11466867426245386 1103 50-51 -0.17791334480622112 1103 52-53 -0.09240779227451412 1103 54-55 0.03069704424388675 1103 56-57 -0.047837582229064424 1103 58-59 -0.12735088993077426 1103 60-61 0.04960394033226834 1103 62-63 0.04094169156880412 1103 64-65 -0.023357720017095573 1103 66-67 -0.0011357805529996767 1103 68-69 0.09268927019031281 1103 70-71 0.07138941908015539 1103 72-73 0.16133450314005415 1103 74-75 0.22633943829253766 1103 76-77 0.1257253787669761 1103 78-79 0.11773374370804746 1103 80-81 0.05562933143401949 1103 82-83 -0.026556780366782107 1103 84-85 -0.07896885270868026 1103 86-87 0.1844992272212984 1103 88-89 0.1633277274607181 1103 90-91 0.08599733049785385 1103 92-93 -0.15161834147018993 1103 94-95 -0.17121869802171474 1103 96-97 0.053265996283158756 1103 98-99 0.2809190413889233 1103 100-101 0.7390332416301106 1104 1 0.04644060362799962 1104 2 -0.030813631367209382 1104 3 -0.02060686866139605 1104 4 -0.1216164598260221 1104 5 -0.01181757766185143 1104 6 0.033378883371732115 1104 7 0.0590383075527825 1104 8 -0.02829884242655112 1104 9 0.04580829630409511 1104 10-11 -0.012290754632481082 1104 12-13 0.04549597915080028 1104 14-15 0.17773805799211573 1104 16-17 0.03201739655744262 1104 18-19 0.1226082616011297 1104 20-21 0.12308290622745233 1104 22-23 0.01715207375742267 1104 24-25 0.00763544222392909 1104 26-27 0.05272712269896829 1104 28-29 -0.012186778075012228 1104 30-31 -0.07781586022519349 1104 32-33 0.03249774802709027 1104 34-35 0.06083317619783202 1104 36-37 0.17778573172792278 1104 38-39 0.2189207956983239 1104 40-41 0.2086530853946229 1104 42-43 0.11765773236636079 1104 44-45 0.439345004769919 1104 46-47 0.6411447488004569 1104 48-49 0.5971645025894858 1104 50-51 0.5251718089717983 1104 52-53 0.5383114830630902 1104 54-55 0.6221813080072067 1104 56-57 0.673649087264323 1104 58-59 0.7279300038285115 1104 60-61 0.7573839575076207 1104 62-63 0.8790314196074576 1104 64-65 0.8645625632391685 1104 66-67 0.83043528346915 1104 68-69 0.7590921007288713 1104 70-71 0.8074373970545494 1104 72-73 0.8196845687020016 1104 74-75 0.9058766905271121 1104 76-77 0.7644938347331518 1104 78-79 0.8051053265329493 1104 80-81 0.9267242633134849 1104 82-83 0.9899021241341686 1104 84-85 0.9797467741843064 1104 86-87 1.1996141066301256 1104 88-89 0.8984400851752774 1104 90-91 1.0532276394075097 1104 92-93 1.1529983198867235 1104 94-95 1.3353768186335877 1104 96-97 1.1578543202061766 1104 98-99 1.0884757115127321 1104 100-101 1.5257145633605553 1105 1 0.14694320813585193 1105 2 0.03794582776257016 1105 3 -0.044192686404578296 1105 4 0.03914876400804701 1105 5 -0.04253360272657147 1105 6 0.04321502300768287 1105 7 0.09821932197809247 1105 8 0.03298863831635401 1105 9 0.04654989207904947 1105 10-11 -0.016771840890704937 1105 12-13 0.05346141351404299 1105 14-15 -0.011569567591244834 1105 16-17 -0.011650916091930696 1105 18-19 0.076349785091395 1105 20-21 0.18026167982728225 1105 22-23 0.21417405733976835 1105 24-25 0.07245233606030155 1105 26-27 0.2803990586185847 1105 28-29 0.15248836999566606 1105 30-31 0.2915659249356608 1105 32-33 0.26270224594212976 1105 34-35 0.23870954226558894 1105 36-37 0.30804783150790627 1105 38-39 0.3946837380467514 1105 40-41 0.36497980317259504 1105 42-43 0.4079170311144864 1105 44-45 0.40140163982709254 1105 46-47 0.26095403015506946 1105 48-49 0.4674558324923055 1105 50-51 0.32905191577243187 1105 52-53 0.42017683475070555 1105 54-55 0.3699643138758191 1105 56-57 0.45644600793638546 1105 58-59 0.5826156331797137 1105 60-61 0.6525862868818635 1105 62-63 0.6173566510314146 1105 64-65 0.6181653904742319 1105 66-67 0.4850024336902976 1105 68-69 0.3722478019581672 1105 70-71 0.2908802492278326 1105 72-73 0.4523686574856711 1105 74-75 0.4575610368250089 1105 76-77 0.4597676581868235 1105 78-79 0.6200290661815551 1105 80-81 0.6258419382763272 1105 82-83 0.754857827170575 1105 84-85 0.77931238835572 1105 86-87 0.7445706680472668 1105 88-89 0.7470547038509316 1105 90-91 0.8165250864024998 1105 92-93 0.7004767135401266 1105 94-95 0.5952614116030013 1105 96-97 0.7822981798473734 1105 98-99 1.176818562330677 1105 100-101 1.382705735592392 1106 1 -0.13524619013007566 1106 2 -0.030051680154983273 1106 3 -0.1514950071563561 1106 4 0.10816906409630889 1106 5 0.03419138559178947 1106 6 -0.06305671781574063 1106 7 -0.2894812127812614 1106 8 -0.34112485527172254 1106 9 -0.44322478213308614 1106 10-11 -0.7181308045997596 1106 12-13 -0.41455296787840723 1106 14-15 -0.4252461679754447 1106 16-17 -0.2798820567388347 1106 18-19 -0.4422694454781535 1106 20-21 -0.30897304318814633 1106 22-23 -0.29851868966195383 1106 24-25 -0.31431756166048075 1106 26-27 -0.44766542401471554 1106 28-29 -0.17279847907759205 1106 30-31 -0.2920771880906585 1106 32-33 -0.23659296199100055 1106 34-35 -0.055740382452754034 1106 36-37 -0.4164200050530127 1106 38-39 -0.3144746649437451 1106 40-41 -0.2102784907892996 1106 42-43 -0.07267977071304443 1106 44-45 -0.011121074466039715 1106 46-47 0.020825012970085766 1106 48-49 0.1990676387328989 1106 50-51 -0.04538606771752285 1106 52-53 -0.03906463765466839 1106 54-55 -0.15763420125839644 1106 56-57 -0.04765294850408708 1106 58-59 0.007570204593456253 1106 60-61 -0.06057502353815636 1106 62-63 -0.12253354158579555 1106 64-65 -0.020712393649127137 1106 66-67 0.061814385302362496 1106 68-69 -0.22692539777185772 1106 70-71 0.02851199212799571 1106 72-73 -0.08825358435016284 1106 74-75 0.22410093610334414 1106 76-77 0.3102258379282752 1106 78-79 0.2454392214175236 1106 80-81 0.002169750954799099 1106 82-83 0.030199190547349986 1106 84-85 -0.016682108295544396 1106 86-87 -0.1138790489975392 1106 88-89 0.10741242151875952 1106 90-91 0.1649597471158586 1106 92-93 0.38142007058285543 1106 94-95 0.5499509396648854 1106 96-97 0.5759031328612352 1106 98-99 0.6194266823659795 1106 100-101 0.8443056006045957 1107 1 -0.03822043274435316 1107 2 0.06149935002642337 1107 3 0.06294292541151236 1107 4 0.02396669929692763 1107 5 0.12549898054293607 1107 6 -0.07444287336343791 1107 7 0.11218520683950572 1107 8 0.09104337158482423 1107 9 0.019713201744316677 1107 10-11 0.042316386488344904 1107 12-13 -0.050470211302240386 1107 14-15 -0.00869627800588546 1107 16-17 0.07449550161150142 1107 18-19 0.1683975214442981 1107 20-21 0.19648914168580944 1107 22-23 0.258567726994265 1107 24-25 0.13237325849390658 1107 26-27 0.18699644616076228 1107 28-29 0.10437733018777351 1107 30-31 0.15249084264945623 1107 32-33 0.2355798827235489 1107 34-35 0.11181488931674011 1107 36-37 0.022496565645816702 1107 38-39 0.007770868276331555 1107 40-41 -0.013709103197086847 1107 42-43 0.028204463577687022 1107 44-45 0.02506953474915008 1107 46-47 0.1479207223528931 1107 48-49 0.15432316020879 1107 50-51 0.20215364016020487 1107 52-53 0.08608187956823343 1107 54-55 0.09487426481460659 1107 56-57 0.11577333109866572 1107 58-59 0.12968489850357656 1107 60-61 0.5005255089407754 1107 62-63 0.5326624038208649 1107 64-65 0.5457462793723238 1107 66-67 0.48619325489633525 1107 68-69 0.4063334417205198 1107 70-71 0.5805147742449108 1107 72-73 0.5340084819417541 1107 74-75 0.3265826071978317 1107 76-77 0.3862451873233894 1107 78-79 0.447203699257507 1107 80-81 0.521641718846432 1107 82-83 0.6057821238563577 1107 84-85 0.49653608264432947 1107 86-87 0.594496662209604 1107 88-89 0.46179739124613306 1107 90-91 0.46823757113556397 1107 92-93 0.5626638706809146 1107 94-95 0.7761658353925149 1107 96-97 0.7857366049203449 1107 98-99 0.725692431542182 1107 100-101 0.9566768991918728 1108 1 0.041159853301785176 1108 2 0.037818879205005373 1108 3 0.04167824751535676 1108 4 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-0.25569411872618275 2316 74-75 -0.1472750629766395 2316 76-77 -0.22482102539183657 2316 78-79 0.015949830353182648 2316 80-81 -0.1618350982967307 2316 82-83 -0.48269828515847735 2316 84-85 -0.19203813535935055 2316 86-87 -0.2995536316360692 2316 88-89 -0.11106983942081072 2316 90-91 -0.054080752759556816 2316 92-93 0.01850486540009655 2316 94-95 0.13554310419443283 2316 96-97 0.1596418482887536 2316 98-99 0.14401938357158528 2316 100-101 0.55298969007616 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7023.0 3 1401.0 4 869.0 5 935.0 6 1084.0 7 1359.0 8 1702.0 9 2163.0 10 2995.0 11 4333.0 12 6660.0 13 9627.0 14 11065.0 15 15118.0 16 20505.0 17 29390.0 18 23385.0 19 13246.0 20 11807.0 21 11537.0 22 12257.0 23 13926.0 24 15630.0 25 17750.0 26 20322.0 27 23939.0 28 28374.0 29 34359.0 30 42008.0 31 52834.0 32 67826.0 33 89086.0 34 122192.0 35 174613.0 36 261546.0 37 406769.0 38 400094.0 39 56026.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.901931058082944 20.77056300310768 15.722376080823514 21.605129857985858 2 14.288484641149468 21.452964763351975 41.865447956529 22.393102638969552 3 17.919449906652183 26.729743772047364 31.634295317196138 23.71651100410432 4 11.093133674396356 18.093610512922126 40.871028355235254 29.942227457446265 5 11.682966279218354 38.91053717867952 35.87542319409425 13.531073348007874 6 27.9179836613904 39.08056153090534 19.25971426084578 13.741740546858486 7 25.609198077781986 34.60937414515312 22.622170363584097 17.15925741348079 8 24.640885626473306 34.60602353315629 21.660649101343758 19.092441739026647 9 25.726652821871593 16.74857175820941 22.634847508334726 34.88992791158427 10-11 22.952559116158934 27.611479151658596 29.427939744077193 20.008021988105277 12-13 24.703149373101905 25.811327060498073 29.25887493295357 20.22664863344645 14-15 21.5985227529266 27.086260925949684 28.886923071568017 22.428293249555704 16-17 19.52827683205583 30.06616627509335 29.48067133106413 20.924885561786688 18-19 19.106700985813514 28.576756601927073 32.84239913114741 19.474143281112006 20-21 20.875318830378884 27.17718975774734 32.53393792283329 19.41355348904049 22-23 20.013435356518023 27.586635549545612 32.31506557443483 20.084863519501532 24-25 19.74111778228526 27.640911117038957 32.79583298425063 19.82213811642515 26-27 19.68223730239594 27.720833463863524 32.877223550729504 19.719705683011036 28-29 19.25889159991605 28.129628321823347 32.823374535862314 19.788105542398288 30-31 19.759562841530055 27.731363882205933 32.78263302187657 19.726440254387438 32-33 19.435830843747155 27.774954008760094 32.69195094864548 20.097264198847274 34-35 19.9099856806705 27.94067251608873 32.45650858040684 19.692833222833933 36-37 19.805638125318854 27.583345184262893 32.52893698653569 20.082079703882563 38-39 19.481610818280114 28.2831484731476 32.41620504451636 19.819035664055924 40-41 19.87981804897125 27.979383457790508 32.39739150820617 19.743406985032074 42-43 19.54979581942606 28.179654042899678 31.76865750495689 20.50189263271737 44-45 20.051131904498078 28.10491664022456 31.513983812457663 20.3299676428197 46-47 20.162086710347037 28.327648021812497 30.925491789601118 20.584773478239356 48-49 20.0957526814332 28.451468595746714 30.8602073522302 20.592571370589884 50-51 20.062233306258793 28.84256595004524 30.50921686606161 20.58598387763436 52-53 20.22471714469431 29.18574237624575 30.02341943676441 20.566121042295528 54-55 20.425150515179844 28.568252407409755 30.102662929309282 20.903934148101115 56-57 20.677461963992304 28.974615811274475 29.550031859825715 20.797890364907502 58-59 20.649641475717615 28.76004314173013 29.792601029227058 20.797714353325205 60-61 20.618082636879496 28.817243536668936 30.095704352767093 20.468969473684474 62-63 20.3793795961103 28.7735428110204 30.18190423609642 20.66517335677288 64-65 20.316673518704572 28.89968367943996 29.977773546692703 20.805869255162765 66-67 20.476981267895127 28.847356996221563 29.622382579771333 21.053279156111977 68-69 20.464716597298313 28.639228549667806 29.952885531279392 20.943169321754485 70-71 20.459997086738145 28.587450801420438 30.05613746221087 20.89641464963055 72-73 20.431497444086595 28.266731761835207 30.272360637839846 21.02941015623835 74-75 20.174941202762408 28.850507479635063 29.853857697882752 21.120693619719777 76-77 19.969876530072625 28.60213027331654 30.062407495761555 21.36558570084928 78-79 19.95153097818372 28.445915995150116 30.08348575203183 21.519067274634338 80-81 20.016620875979328 28.586763160223654 29.938334213349798 21.458281750447217 82-83 20.034478001319957 28.628573488231616 30.107448565757405 21.22949994469102 84-85 20.048587963578665 28.46596916034003 29.778659528036023 21.706783348045285 86-87 19.788608075463873 28.580992675913198 29.912409778939914 21.717989469683012 88-89 20.155964735035457 28.141469545883034 29.84192219115313 21.86064352792837 90-91 19.93755056395436 27.93323146369564 30.14205891698288 21.987159055367126 92-93 19.974797764364315 27.909425084253975 30.467111430523943 21.64866572085777 94-95 19.71891767195262 27.784671924779552 30.47256891826856 22.023841484999267 96-97 19.576269838033934 27.760841742236575 30.49088039650709 22.172008023222396 98-99 19.609510426083556 27.818801858167074 30.17480236338484 22.396885352364528 100-101 19.638418882376147 27.368916094319413 30.807026405070975 22.185638618233465 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3261.0 1 1898.0 2 552.5 3 522.5 4 413.0 5 393.0 6 631.0 7 1206.0 8 1874.0 9 2207.0 10 2054.0 11 1836.0 12 1919.5 13 2150.0 14 2355.0 15 2577.5 16 2843.5 17 3221.0 18 3652.0 19 4187.0 20 4928.0 21 5691.0 22 6686.5 23 8054.0 24 9704.5 25 12217.0 26 16782.0 27 22091.5 28 25775.0 29 30133.0 30 36593.5 31 43379.5 32 51392.5 33 59759.5 34 68310.5 35 76139.0 36 82244.0 37 86409.5 38 84722.0 39 81055.0 40 79042.0 41 77147.0 42 75002.0 43 74152.0 44 73808.5 45 72153.0 46 69654.0 47 69909.5 48 77118.0 49 73835.0 50 60566.5 51 52206.5 52 45878.5 53 41006.0 54 36359.5 55 32335.0 56 28163.0 57 23977.0 58 20509.5 59 17062.0 60 14140.0 61 11948.0 62 9765.0 63 7104.0 64 5145.0 65 4022.0 66 3111.0 67 2375.5 68 1854.5 69 1438.0 70 1118.0 71 877.5 72 649.0 73 536.0 74 458.0 75 334.5 76 249.0 77 178.5 78 112.5 79 78.0 80 58.5 81 38.0 82 23.0 83 17.0 84 14.0 85 8.0 86 6.5 87 7.5 88 4.5 89 2.5 90 3.0 91 2.5 92 1.0 93 0.5 94 1.5 95 1.0 96 0.5 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.260745326824622 2 0.2317736238441084 3 0.22150454419176888 4 0.22805294281065205 5 0.22656467039726952 6 0.25722308211295 7 0.25628050958447435 8 0.24705322062150256 9 0.24288605786403142 10-11 0.24985613366670634 12-13 0.2493600428622455 14-15 0.23425407786641267 16-17 0.251418819700758 18-19 0.23971107671548203 20-21 0.23308826447592965 22-23 0.23060781045362544 24-25 0.22505159344366393 26-27 0.23470055959042743 28-29 0.2501289836091598 30-31 0.2504018335516133 32-33 0.2505754653331746 34-35 0.25752073659562646 36-37 0.23147596936143192 38-39 0.23045898321228717 40-41 0.22619260229392388 42-43 0.23179842838433146 44-45 0.25191491050521886 46-47 0.25645414136603567 48-49 0.261340635789975 50-51 0.2609933722268524 52-53 0.2816555542326467 54-55 0.2603732587212763 56-57 0.22852422907488987 58-59 0.21086339643608368 60-61 0.20838294241377942 62-63 0.21552664999801566 64-65 0.20801087431043377 66-67 0.2090278604595785 68-69 0.21212842798745882 70-71 0.21178116442433625 72-73 0.21560106361868475 74-75 0.21820554034210424 76-77 0.23333630987816012 78-79 0.2271351748223995 80-81 0.21996666269794024 82-83 0.21493134103266262 84-85 0.21612195896336867 86-87 0.2242082390760805 88-89 0.22185180775489144 90-91 0.23281541453347623 92-93 0.21979303091637895 94-95 0.21788308131920467 96-97 0.22810255189109813 98-99 0.2354446957971187 100-101 0.2100200420685002 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2015760.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.08369689448157 #Duplication Level Percentage of deduplicated Percentage of total 1 75.57936207127315 41.63190671812287 2 14.047961576917823 15.47627314976529 3 4.80282616512742 7.936722621502922 4 2.063305196399284 4.5461791215706775 5 1.0930472001258402 3.0104540331546756 6 0.6147603128021364 2.0317962439889734 7 0.38221597420270337 1.4737708209847489 8 0.24256919765745927 1.068928652776086 9 0.1779779830818712 0.8823316746575667 >10 0.8517060565481775 8.732614842981558 >50 0.0873926515250738 3.3622906444303053 >100 0.05148611642483382 5.410848094663092 >500 0.0035929986093410035 1.3473602413659165 >1k 0.0015270244089699216 1.5619997598208113 >5k 8.982496523352478E-5 0.3507065253131208 >10k+ 1.7964993046704956E-4 1.175816854901383 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13685 0.6789002659046712 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10079 0.5000099218160892 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7088 0.3516291622018494 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4320 0.21431122752708656 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3154 0.1564670397269516 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3034 0.15051395007342144 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2566 0.12729690042465372 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2378 0.11797039330078977 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 7.441362066912728E-5 0.0 22-23 0.0 0.0 0.0 4.2167718379172123E-4 0.0 24-25 0.0 0.0 0.0 6.697225860221455E-4 0.0 26-27 0.0 0.0 0.0 0.0013890542524903759 0.0 28-29 0.0 0.0 0.0 0.002926935746319006 0.0 30-31 0.0 0.0 0.0 0.006300353216652776 0.0 32-33 0.0 0.0 0.0 0.010566734135016072 0.0 34-35 0.0 0.0 0.0 0.014857919593602413 0.0 36-37 0.0 0.0 0.0 0.02294419970631424 0.0 38-39 0.0 0.0 0.0 0.03403182918601421 0.0 40-41 0.0 0.0 0.0 0.046409294757312376 0.0 42-43 0.0 0.0 0.0 0.05982855101797833 0.0 44-45 0.0 0.0 0.0 0.0702216533714331 0.0 46-47 0.0 0.0 0.0 0.08574929555105766 0.0 48-49 0.0 0.0 0.0 0.10013592888042228 0.0 50-51 0.0 0.0 0.0 0.11596122554272334 0.0 52-53 0.0 0.0 0.0 0.14937294122316147 0.0 54-55 0.0 0.0 0.0 0.20061912132396714 0.0 56-57 0.0 0.0 0.0 0.2821020359566615 0.0 58-59 0.0 0.0 0.0 0.3895056951224352 0.0 60-61 0.0 0.0 0.0 0.5165793546850815 0.0 62-63 0.0 0.0 0.0 0.6049579314997817 0.0 64-65 0.0 0.0 0.0 0.7161566853196809 0.0 66-67 0.0 0.0 0.0 0.8988669286026114 0.0 68-69 0.0 0.0 0.0 1.1306157479064969 0.0 70-71 0.0 0.0 0.0 1.371591856173354 0.0 72-73 0.0 0.0 0.0 1.6496259475334365 0.0 74-75 0.0 0.0 0.0 1.9383756002698735 0.0 76-77 0.0 0.0 0.0 2.2226852403063857 0.0 78-79 0.0 0.0 0.0 2.5080614755724886 0.0 80-81 0.0 0.0 0.0 2.7772155415327218 0.0 82-83 0.0 0.0 0.0 3.056465055363734 0.0 84-85 0.0 0.0 0.0 3.4888329959915865 0.0 86-87 0.0 0.0 0.0 4.066233083303568 0.0 88-89 0.0 0.0 0.0 4.755749692423701 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 18045 0.0 24.91706 1 GTACATG 10045 0.0 24.415314 1 GTGTAGC 1410 0.0 24.270344 1 CATTCCG 395 0.0 24.053562 9 TACATGG 10175 0.0 23.813623 2 GGAGCGT 200 2.0516381E-8 23.754663 6 TAGGACG 410 0.0 23.17701 4 GAGTACT 10865 0.0 23.018229 12-13 ACATGGG 10260 0.0 23.014923 3 GGATCGA 125 2.2537216E-4 22.804478 6 GGTATCA 13370 0.0 22.787106 1 TCAACGC 20785 0.0 21.396223 4 ATCAACG 20835 0.0 21.344343 3 CAACGCA 20840 0.0 21.338694 5 GTACTTT 11700 0.0 21.314047 14-15 TATCAAC 21075 0.0 21.21347 2 AGTACTT 11210 0.0 21.208097 12-13 TAGCCCT 2100 0.0 21.041416 4 GGACGTG 455 0.0 20.88322 6 AACGCAG 21335 0.0 20.84309 6 >>END_MODULE