FastQCFastQC Report
Thu 26 May 2016
SRR937472_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937472_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2015760
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155370.7707762828908203No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124300.6166408699448347No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87080.43199587252450683No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT57350.2845080763582966No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29800.14783505972933286No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT26040.12918204548160495No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT23070.11444814858911775No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA217200.024.4190251
GTACATG105250.023.0739961
GAGTACT130000.022.85785712-13
AGTACTT133050.022.67307512-13
TAGCCCG1050.00219698922.6186985
TACATGG107600.022.2045862
GGTATCA161750.022.1539441
GTACTTT136000.021.79324214-15
ACATGGG108300.021.0961653
TCAACGC254800.020.7483084
ATCAACG255300.020.707673
CAACGCA255450.020.6955135
TATCAAC257700.020.5885452
AACGCAG261000.020.3095286
AGAGTAC207850.020.23904810-11
CATTCCG4300.019.8863749
TACTTTT155700.019.32565114-15
ACGCAGA280550.018.8806157
ACTTTTT159650.018.77312716-17
CGCAGAG281700.018.7698128
Produced by FastQC (version 0.11.5)