FastQCFastQC Report
Thu 26 May 2016
SRR937471_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937471_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1147336
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133141.1604272854682498No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95560.8328859200792096No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68290.5952048920281416No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT28340.24700697964676432No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT22210.19357886443029765No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22120.1927944385951456No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18810.1639449995467762No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18520.16141740518906406No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT17240.1502611266446795No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14300.12463654936304622No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC12240.10668191358067733No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA121550.035.359161
GGTATCA75700.034.29141
ATCAACG149750.028.6537553
TCAACGC150850.028.509064
TATCAAC150250.028.4963952
CAACGCA153350.027.9810895
AACGCAG156350.027.4661836
TATAACG700.00837404227.1544842
GTACATG86100.024.7931481
ACGCAGA173900.024.5303947
CGCAGAG173650.024.4562858
GAGTACT90450.024.05070112-13
TACATGG88400.024.0289542
GCAGAGT176750.023.2748159
ACATGGG88500.023.2490543
GTACTTT94650.022.88261214-15
AGTACTT91950.022.6252412-13
CATGGGT8750.022.2666784
CTATTAC1954.1846033E-721.943021
AGAGTAC140950.021.93843810-11
Produced by FastQC (version 0.11.5)