FastQCFastQC Report
Thu 26 May 2016
SRR937471_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937471_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1147336
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132771.1572024237015137No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105560.920044346207214No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76600.6676335441405133No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT42920.3740839649413947No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25790.2247815809841232No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22740.1981982610150819No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20280.17675728818759281No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT15850.1381461054128869No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT15780.13753599642999087No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATC600.00395240531.6642846
GTATCAA143250.030.1269441
GGTATCA98750.028.8305931
CCCGCGA700.00839286127.1419964
GTATAGG4350.025.1305751
GTACATG87050.024.4609381
TATCAAC178000.024.2562542
ATCAACG178950.024.0743983
TCAACGC179600.023.9608234
CAACGCA182500.023.5800765
TACATGG89400.023.4835952
AACGCAG184650.023.3302236
GAGTACT101450.023.20114112-13
AGTACTT103050.023.18663412-13
ACATGGG88650.022.6106763
GACTGCG1302.9403996E-421.9242957
GTACTTT106450.021.86308114-15
AGAGTAC157650.021.39351310-11
CGCAGAG202350.021.2687858
ACGCAGA202750.021.2502547
Produced by FastQC (version 0.11.5)