FastQCFastQC Report
Thu 26 May 2016
SRR937470_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937470_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1138572
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135121.1867497180678956No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96980.8517687067660191No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71430.6273648043338498No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT29220.25663726141166304No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22690.1992847180503297No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT21670.190326127816247No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19200.16863228675920364No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT16690.14658712843807858No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15920.13982427110450635No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14780.12981172907817864No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC13480.11839391799552423No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA120950.032.0524021
GGTATCA79950.031.7710531
CGTTCGC650.00584510229.2296856
GTACATG84800.025.9713151
TCAACGC151800.025.5238324
ATCAACG151800.025.494763
TATCAAC152900.025.4357242
CAACGCA152600.025.3877875
AACGCAG155600.024.9701546
TACATGG87450.024.7371642
GAGTACT85200.024.02892312-13
ACATGGG85750.023.841453
ATGGGTA5400.022.8876575
AGTACTT87900.022.66938412-13
GTACTTT90950.022.50928314-15
ACGCAGA172750.022.3262357
CGCAGAG173150.022.2756398
GTAAGGT3007.2759576E-1222.1853774
AGAGTAC136800.021.8404310-11
CATGGGT9300.021.4697194
Produced by FastQC (version 0.11.5)