##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937470_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1138572 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.791165600418772 33.0 31.0 34.0 30.0 34.0 2 31.872182874688644 33.0 31.0 34.0 30.0 34.0 3 31.883794788559705 33.0 31.0 34.0 30.0 34.0 4 35.528840512501624 37.0 35.0 37.0 33.0 37.0 5 35.55622832811627 37.0 35.0 37.0 33.0 37.0 6 35.62609654901051 37.0 35.0 37.0 33.0 37.0 7 35.57509933495642 37.0 35.0 37.0 33.0 37.0 8 35.575768594344495 37.0 35.0 37.0 33.0 37.0 9 37.18800304240751 39.0 37.0 39.0 34.0 39.0 10-11 37.06433058251916 39.0 37.0 39.0 33.0 39.0 12-13 36.94568547267981 39.0 37.0 39.0 33.0 39.0 14-15 38.04901490639151 40.0 38.0 41.0 33.0 41.0 16-17 38.04209966519465 40.0 38.0 41.0 33.0 41.0 18-19 38.113349880376475 40.0 38.0 41.0 33.5 41.0 20-21 38.10124304830964 40.0 38.0 41.0 33.0 41.0 22-23 37.94994168133417 40.0 38.0 41.0 33.0 41.0 24-25 37.78676930400537 40.0 37.5 41.0 33.0 41.0 26-27 37.616969765636256 40.0 37.0 41.0 32.5 41.0 28-29 37.64102489785451 40.0 37.0 41.0 33.0 41.0 30-31 37.40088022540516 39.5 37.0 41.0 32.5 41.0 32-33 37.10523928218856 39.0 36.0 41.0 32.0 41.0 34-35 36.92027425582221 39.0 35.0 41.0 31.0 41.0 36-37 36.85995571645886 39.0 35.0 41.0 31.0 41.0 38-39 36.73006142782363 39.0 35.0 41.0 31.0 41.0 40-41 36.51190043317419 39.0 35.0 41.0 30.5 41.0 42-43 36.17771076400965 39.0 35.0 41.0 30.0 41.0 44-45 35.799828205857864 39.0 35.0 41.0 28.5 41.0 46-47 35.24309090685525 39.0 35.0 41.0 25.0 41.0 48-49 34.754539458198515 39.0 35.0 40.5 23.0 41.0 50-51 33.82119883503195 38.0 34.0 39.5 21.0 40.5 52-53 33.8887584623546 38.0 34.0 39.5 20.0 40.5 54-55 34.19344670341445 38.0 34.0 40.0 20.0 41.0 56-57 33.98808419669551 38.0 34.0 40.0 18.5 41.0 58-59 33.755744915560896 38.0 34.0 40.0 16.0 41.0 60-61 33.47390239703769 38.0 34.0 40.0 13.5 41.0 62-63 33.154622193414205 37.0 33.0 40.0 9.0 41.0 64-65 32.79489132000435 37.0 33.0 40.0 7.5 41.0 66-67 32.409703558492566 36.0 32.5 39.0 4.5 41.0 68-69 31.938461511437133 36.0 32.0 39.0 2.0 40.5 70-71 31.49596380378228 35.0 31.0 39.0 2.0 40.0 72-73 31.113747308031463 35.0 31.0 38.0 2.0 40.0 74-75 30.676345896438697 35.0 31.0 37.0 2.0 39.5 76-77 30.245852260550933 35.0 30.0 37.0 2.0 39.0 78-79 29.775561844134582 35.0 30.0 36.0 2.0 39.0 80-81 29.283852931566912 34.0 29.0 36.0 2.0 37.5 82-83 28.813767596603462 34.0 29.0 35.0 2.0 37.0 84-85 28.375066750280176 34.0 28.5 35.0 2.0 37.0 86-87 27.959067586415266 34.0 27.5 35.0 2.0 36.0 88-89 27.55156722631507 34.0 26.5 35.0 2.0 36.0 90-91 27.129890336316016 33.5 26.0 35.0 2.0 35.5 92-93 26.692108623784883 33.0 25.0 35.0 2.0 35.0 94-95 26.179663648851367 33.0 24.5 35.0 2.0 35.0 96-97 25.58841074609247 33.0 21.5 35.0 2.0 35.0 98-99 24.87903619621772 32.0 12.5 34.5 2.0 35.0 100-101 23.03652206448077 30.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.24701599229015514 1101 2 0.20809279518751111 1101 3 0.2008684480437637 1101 4 0.13940453501789563 1101 5 0.13335900680093715 1101 6 0.1026437595477816 1101 7 0.12302244078652791 1101 8 0.06884812837797938 1101 9 0.31152107253446815 1101 10-11 0.237447494651029 1101 12-13 0.20557334205231825 1101 14-15 0.28849590879672604 1101 16-17 0.35133401459495417 1101 18-19 0.30743460797017264 1101 20-21 0.2817999087437215 1101 22-23 0.29347459871893733 1101 24-25 0.3594658862942097 1101 26-27 0.30703824660306367 1101 28-29 0.3464766260432981 1101 30-31 0.35367995715701284 1101 32-33 0.4629238447949291 1101 34-35 0.42962622176189313 1101 36-37 0.5937499769845118 1101 38-39 0.59128822704767 1101 40-41 0.5807578792033468 1101 42-43 0.7078401518528921 1101 44-45 0.7693507954938426 1101 46-47 0.8741630187148886 1101 48-49 0.9445219513888006 1101 50-51 1.033760111896875 1101 52-53 1.0617630610624715 1101 54-55 1.3105139992797916 1101 56-57 1.0673646985517848 1101 58-59 1.2236034611449327 1101 60-61 1.1709557180082157 1101 62-63 1.1339423439116132 1101 64-65 1.1985070273930774 1101 66-67 1.07811444732004 1101 68-69 1.26550372683624 1101 70-71 1.2288216941695573 1101 72-73 1.0124332754742333 1101 74-75 0.9618720378244312 1101 76-77 1.1449952190427553 1101 78-79 1.1317369919799596 1101 80-81 0.8709320853380298 1101 82-83 0.9210107049440133 1101 84-85 0.9810533559808725 1101 86-87 1.1634696867813794 1101 88-89 1.2965123847483007 1101 90-91 1.2403323848902517 1101 92-93 1.4548095397631151 1101 94-95 1.3548905830278848 1101 96-97 1.5001369785563234 1101 98-99 1.8717074297525933 1101 100-101 2.178299173662513 1102 1 0.11592278213543139 1102 2 0.15984940477141052 1102 3 0.29332986084232715 1102 4 0.17872404831216926 1102 5 0.12815536777434744 1102 6 0.09926855299828219 1102 7 0.1927704504004666 1102 8 0.14687220078296548 1102 9 0.23053019826678423 1102 10-11 0.28042035216943617 1102 12-13 0.3087741551062493 1102 14-15 0.3444356151208936 1102 16-17 0.3694528650531197 1102 18-19 0.34990986897363285 1102 20-21 0.2780679348816264 1102 22-23 0.22424883073035318 1102 24-25 0.2765937685985449 1102 26-27 0.2636405543242546 1102 28-29 0.2984468265840903 1102 30-31 0.19583624001716515 1102 32-33 0.27906662682887884 1102 34-35 0.44536857943051444 1102 36-37 0.621079057275935 1102 38-39 0.659976496913977 1102 40-41 0.6374756668704649 1102 42-43 0.5660709145838467 1102 44-45 0.6514462401385828 1102 46-47 0.7284975068476811 1102 48-49 0.5654296894840414 1102 50-51 0.7164343645632414 1102 52-53 0.7348480186259394 1102 54-55 0.6565677303147908 1102 56-57 0.6014406712121456 1102 58-59 0.6382926510953624 1102 60-61 0.6839636419979982 1102 62-63 0.553897372453001 1102 64-65 0.9196077014955293 1102 66-67 0.9161338723613497 1102 68-69 0.9513536780154475 1102 70-71 1.0577799336167466 1102 72-73 0.7316198460015002 1102 74-75 0.7355254759346899 1102 76-77 0.9870012840870714 1102 78-79 0.8358325398994424 1102 80-81 0.8016887136991535 1102 82-83 0.9007946729476224 1102 84-85 0.9475336586697729 1102 86-87 0.787723292008959 1102 88-89 0.7044863689003193 1102 90-91 0.8779565493036507 1102 92-93 0.9636231006764397 1102 94-95 1.075797616977109 1102 96-97 1.1278549821615513 1102 98-99 1.0744263722191683 1102 100-101 1.277403030504935 1103 1 0.1706520413680721 1103 2 0.07898153756584492 1103 3 0.10814421675659247 1103 4 0.11320096639484234 1103 5 0.1506567988556995 1103 6 0.13499726457901318 1103 7 0.0869831821922844 1103 8 0.19441507746712006 1103 9 0.22268774855287177 1103 10-11 0.13153585220536712 1103 12-13 0.14724100234110438 1103 14-15 0.3160069672263788 1103 16-17 0.17599916394220827 1103 18-19 -0.01660865082981644 1103 20-21 -0.0927967975743087 1103 22-23 -0.2671804788069707 1103 24-25 -0.18099294501914898 1103 26-27 0.12133407245499939 1103 28-29 0.03450090782985882 1103 30-31 0.07712277595774708 1103 32-33 0.04732561414367353 1103 34-35 0.08017458427264756 1103 36-37 0.3360380081632144 1103 38-39 0.398833267353659 1103 40-41 0.2941285844178765 1103 42-43 0.3885446533117545 1103 44-45 0.36198618527426873 1103 46-47 0.23633097358339938 1103 48-49 0.29078318516354074 1103 50-51 0.3172884289581859 1103 52-53 0.31331340380365447 1103 54-55 0.24897810497313344 1103 56-57 0.41959204864343747 1103 58-59 0.4418212888298214 1103 60-61 0.2842508494936453 1103 62-63 0.1685653987502036 1103 64-65 0.28249585908050534 1103 66-67 0.13196788922704883 1103 68-69 0.21364425072057358 1103 70-71 0.25740060413102483 1103 72-73 0.39829064277308035 1103 74-75 0.49810505273110195 1103 76-77 0.6941964637590239 1103 78-79 0.6380917898889109 1103 80-81 0.4253586134119445 1103 82-83 0.5311782647016194 1103 84-85 0.444270049767578 1103 86-87 0.6528472818885511 1103 88-89 0.6277500521444033 1103 90-91 0.697716809103774 1103 92-93 0.84841423714089 1103 94-95 0.7273615225034398 1103 96-97 0.6924168854130173 1103 98-99 0.9228287030851767 1103 100-101 1.187241188213818 1104 1 0.2446730338301748 1104 2 0.15341321058771484 1104 3 0.29552080366686795 1104 4 0.23818872954677062 1104 5 0.1925820311170483 1104 6 0.19747025397533946 1104 7 0.23364807401854648 1104 8 0.29855304225842616 1104 9 0.32548383849191964 1104 10-11 0.415156144600374 1104 12-13 0.34725109027927203 1104 14-15 0.4206355997794944 1104 16-17 0.4838457293872551 1104 18-19 0.34082968142508463 1104 20-21 0.37649975676804104 1104 22-23 0.34836789912420585 1104 24-25 0.4741046274289573 1104 26-27 0.38681756524540845 1104 28-29 0.34652411844452047 1104 30-31 0.597680083803418 1104 32-33 0.5112394476318087 1104 34-35 0.6054272602624025 1104 36-37 0.5782753695883684 1104 38-39 0.6343084271489872 1104 40-41 0.6646215443502612 1104 42-43 0.7722161017009128 1104 44-45 0.8678342048150256 1104 46-47 0.9475941863947313 1104 48-49 1.0832868323411802 1104 50-51 0.857784934591912 1104 52-53 0.9986852368476775 1104 54-55 1.0815135560173843 1104 56-57 1.0093923074676923 1104 58-59 0.959237009777155 1104 60-61 1.0012811992645467 1104 62-63 0.8823434330706803 1104 64-65 0.9656438688317834 1104 66-67 1.1816083049694797 1104 68-69 1.1750623641209437 1104 70-71 1.063801430745034 1104 72-73 1.1229955855046363 1104 74-75 1.0221003179662738 1104 76-77 1.130383390268694 1104 78-79 1.2231044565189677 1104 80-81 1.2885011076277983 1104 82-83 1.2904540940017668 1104 84-85 1.2565884370345692 1104 86-87 1.419643698939435 1104 88-89 1.330793983025906 1104 90-91 1.174025077392784 1104 92-93 1.4505193928532876 1104 94-95 1.6319770825212991 1104 96-97 1.652397616854195 1104 98-99 1.4737844307657646 1104 100-101 1.7032010227000036 1105 1 0.1436429764171372 1105 2 0.25501739836211357 1105 3 0.21728709883741715 1105 4 0.05051564612900705 1105 5 0.1435276575945892 1105 6 0.14500487065888734 1105 7 0.1974272026912942 1105 8 0.1243044775843245 1105 9 0.1425726598360555 1105 10-11 0.060934597825635706 1105 12-13 0.14910012776660153 1105 14-15 0.2426129722887893 1105 16-17 0.22526754634098722 1105 18-19 0.219612187335251 1105 20-21 0.24043086112467904 1105 22-23 0.2952355114173457 1105 24-25 0.29803235454817667 1105 26-27 0.33875947676178697 1105 28-29 0.32599051493218667 1105 30-31 0.29083273493478856 1105 32-33 0.38336035273142954 1105 34-35 0.38919963446031147 1105 36-37 0.21986604841308832 1105 38-39 0.6038626318095766 1105 40-41 0.5447211728541461 1105 42-43 0.6099482867735233 1105 44-45 0.602535319303378 1105 46-47 0.44392988379425447 1105 48-49 0.4047154037697567 1105 50-51 0.3117363023730704 1105 52-53 0.3514207991577081 1105 54-55 0.5322649913432826 1105 56-57 0.4432575556946432 1105 58-59 0.5273981040020814 1105 60-61 0.578197781500279 1105 62-63 0.5392498835941595 1105 64-65 0.5478373845359314 1105 66-67 0.5841412330343303 1105 68-69 0.7348985681060789 1105 70-71 0.6716788370266968 1105 72-73 0.7862427992837588 1105 74-75 0.8870952521101465 1105 76-77 0.7877035523760867 1105 78-79 0.895799491979961 1105 80-81 0.9598705774015208 1105 82-83 0.9190761811344927 1105 84-85 0.9943966099491242 1105 86-87 1.096306988368685 1105 88-89 1.0765471069705228 1105 90-91 1.0559821864775536 1105 92-93 1.2338273969059728 1105 94-95 1.4532536782659768 1105 96-97 1.4978552325245786 1105 98-99 1.236389969435134 1105 100-101 1.5626741736625114 1106 1 0.07121189872290401 1106 2 -0.01457324491273937 1106 3 0.07238317235454161 1106 4 0.14399935540218678 1106 5 0.03262540195549235 1106 6 0.030719156373173462 1106 7 0.033266801688782266 1106 8 -0.08922935700213941 1106 9 -0.057552653447153546 1106 10-11 -0.4374363294926624 1106 12-13 -0.3323535063186114 1106 14-15 -0.3005156826819686 1106 16-17 -0.15971574522124854 1106 18-19 -0.18391747014177184 1106 20-21 0.030677310581651795 1106 22-23 -0.023561481063858025 1106 24-25 -0.09465770393135386 1106 26-27 -0.09139925339693633 1106 28-29 0.1806062208636945 1106 30-31 0.11207333643854867 1106 32-33 0.23093762925607564 1106 34-35 0.03665159770174142 1106 36-37 0.22623614030949568 1106 38-39 0.14066296597832917 1106 40-41 0.03860170752414405 1106 42-43 0.129956640992404 1106 44-45 0.18824960501765986 1106 46-47 0.4112022313297601 1106 48-49 0.5415157379385107 1106 50-51 0.3729936123145876 1106 52-53 0.14337986348519394 1106 54-55 0.1375490347852235 1106 56-57 0.24705872528528516 1106 58-59 0.18736051001711473 1106 60-61 -0.02769194281050602 1106 62-63 0.1477920723995041 1106 64-65 0.33825091837051957 1106 66-67 0.3660543491579702 1106 68-69 0.22092613702838193 1106 70-71 0.1912277092071477 1106 72-73 0.31955524706988925 1106 74-75 0.08101755787455645 1106 76-77 0.30313797175787727 1106 78-79 0.24943357719299186 1106 80-81 0.2623141864240779 1106 82-83 0.17715888790140966 1106 84-85 -0.0050185947292398225 1106 86-87 0.12548409780895042 1106 88-89 0.13818035676166573 1106 90-91 0.10437399934305347 1106 92-93 0.12000116465868871 1106 94-95 0.46622360307801003 1106 96-97 0.5207875633516466 1106 98-99 0.7001326594936081 1106 100-101 0.6290484426683598 1107 1 0.12271945549361618 1107 2 0.14405058739530574 1107 3 0.27060600215632036 1107 4 0.14330063891399902 1107 5 0.10117556524249949 1107 6 0.15250847816249546 1107 7 0.032492137756229056 1107 8 0.1664401197199723 1107 9 0.27107193833533927 1107 10-11 0.2562448431358817 1107 12-13 0.16858064724712563 1107 14-15 0.2632118178876297 1107 16-17 0.43951718559063124 1107 18-19 0.15290944563250974 1107 20-21 0.14032263601645667 1107 22-23 0.4223639385457716 1107 24-25 0.2139775204933443 1107 26-27 0.3021072401095566 1107 28-29 0.24889816283984345 1107 30-31 0.5306956497977069 1107 32-33 0.5397095590806344 1107 34-35 0.45788650314717927 1107 36-37 0.3721387756858192 1107 38-39 0.4941223720693202 1107 40-41 0.4845119376449034 1107 42-43 0.4652873921126286 1107 44-45 0.6293751461431967 1107 46-47 0.922898137762509 1107 48-49 0.772897221951574 1107 50-51 0.9029700686068409 1107 52-53 0.9558580285949461 1107 54-55 0.9972361313144233 1107 56-57 0.9893614517985441 1107 58-59 0.8651686667726466 1107 60-61 0.8378658911683843 1107 62-63 0.868723188067456 1107 64-65 1.0581187698173196 1107 66-67 1.1847905836836716 1107 68-69 1.0746893328968419 1107 70-71 1.072544637892495 1107 72-73 0.7547492927902546 1107 74-75 0.7315757715906663 1107 76-77 0.48564727531981333 1107 78-79 0.6555397517202195 1107 80-81 0.8789542714852168 1107 82-83 0.758794795853106 1107 84-85 0.9482205637730772 1107 86-87 0.7850876521493468 1107 88-89 0.5527015081249225 1107 90-91 0.7984425039378706 1107 92-93 0.7132646263432036 1107 94-95 0.5847471847594861 1107 96-97 0.7947527794221259 1107 98-99 0.6534173864625465 1107 100-101 0.7334533944417352 1108 1 0.18789027193831842 1108 2 0.15098595563640416 1108 3 0.0343960868062787 1108 4 0.15020981254291854 1108 5 0.12899424337964405 1108 6 0.19993864884107637 1108 7 0.08512814294000037 1108 8 0.22676631157482063 1108 9 0.3211036476027118 1108 10-11 0.25229290335591514 1108 12-13 0.2444557540935648 1108 14-15 0.3225846637254435 1108 16-17 0.24623610361528137 1108 18-19 0.37179472701779304 1108 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2316 98-99 -3.8947539823984734 2316 100-101 -4.588836499572299 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4059.0 3 790.0 4 712.0 5 779.0 6 937.0 7 1103.0 8 1416.0 9 1750.0 10 2285.0 11 3113.0 12 4655.0 13 6640.0 14 7690.0 15 10504.0 16 14159.0 17 19566.0 18 15935.0 19 8706.0 20 8084.0 21 8067.0 22 8343.0 23 9269.0 24 10047.0 25 11210.0 26 12760.0 27 14918.0 28 17301.0 29 20921.0 30 25423.0 31 31401.0 32 39935.0 33 52500.0 34 71853.0 35 104896.0 36 150939.0 37 217191.0 38 191274.0 39 27436.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.07266760142099 19.72457942665072 15.802488937614404 20.400264034313885 2 14.750556057169284 21.252262767292827 41.16893126913167 22.82824990640622 3 18.315585860392027 27.631269494106736 30.97952133352242 23.073623311978817 4 11.463982910876698 17.511682706071483 39.0118258495012 32.012508533550616 5 11.911655762961344 39.02986238164408 34.00264789590933 15.055833959485254 6 28.392840639536676 38.12443063544385 18.248271552236307 15.234457172783165 7 26.237078289766657 35.36231833039095 21.260602710984926 17.140000668857468 8 26.43919938181095 33.366014740077205 20.308795288244728 19.885990589867124 9 27.22729232854289 16.387541912715946 21.235248044073256 35.14991771466791 10-11 23.893746107684283 27.814383578223715 27.121105758756748 21.17076455533525 12-13 26.04471552765511 25.8460678632174 28.07541922182396 20.033797387303537 14-15 22.763224828688458 26.100213512357488 28.55591365863018 22.580648000323876 16-17 19.726514111049845 29.937877320359313 28.722707188179747 21.612901380411092 18-19 19.678875849860443 27.01367037185506 33.440861681816116 19.866592096468374 20-21 21.067617192896925 25.646080047309244 33.49927355320046 19.787029206593374 22-23 20.47229300329966 26.007664156975736 33.61237529464411 19.907667545080493 24-25 20.213841536223796 26.185864917827374 33.74335319930767 19.85694034664116 26-27 19.842577886108753 26.34774536159129 33.81095267217219 19.998724080127765 28-29 19.47055153009182 26.651479134231444 33.93194565579953 19.946023679877207 30-31 19.691519175858804 26.139232261494428 33.86133106126487 20.3079175013819 32-33 19.49149782604869 26.511380239046627 33.660599552887746 20.336522382016934 34-35 20.19748444114658 26.37670711682486 33.43618614554575 19.989622296482803 36-37 20.218062778848438 26.075837975307337 33.402984943284316 20.303114302559905 38-39 19.772400290384322 26.639945442946082 33.398684471038564 20.18896979563104 40-41 19.81733540586095 26.698561183047804 33.283074685348154 20.20102872574309 42-43 19.787336273747126 26.78312453579361 32.63762509372129 20.79191409673797 44-45 19.915665359654284 26.845133773326125 32.43878302201035 20.800417845009246 46-47 20.549511285408265 27.256949639426676 31.74801055405256 20.445528521112504 48-49 20.417388763368507 27.649783172953786 31.06717457679779 20.865653486879918 50-51 20.324606795274764 27.880897410732913 30.97007075880656 20.82442503518576 52-53 20.541843493357362 28.19215736852832 30.384991744297267 20.88100739381705 54-55 20.149545361330144 28.04664238054458 30.513423524679695 21.29038873344558 56-57 20.61066754421933 28.205208298503997 30.088632232110335 21.095491925166346 58-59 20.9321048866567 27.608726065835288 30.30937885777688 21.14979018973113 60-61 20.551652109950318 27.91480042568112 30.96759746650225 20.565949997866316 62-63 20.408080658757772 27.959895885579733 31.100751292190893 20.531272163471606 64-65 20.20819576138348 28.07769990404975 30.845871169743955 20.868233164822815 66-67 20.342899175042938 28.12249239800659 30.364244587099137 21.17036383985134 68-69 20.34436416026043 27.99480902262254 30.62192748028638 21.038899336830642 70-71 20.54974904616424 28.045543017640682 30.483059736164105 20.921648200030972 72-73 20.424762268460874 28.066136311441298 30.47878036483113 21.030321055266697 74-75 20.031819515764344 28.807089345873997 30.12809203020566 21.032999108155995 76-77 20.189550104498956 28.287009129908704 30.380244197558028 21.14319656803432 78-79 20.21344559407674 28.20498379974692 30.193936652698603 21.38763395347773 80-81 20.32085279155656 28.579367111664904 29.859364014188554 21.240416082589984 82-83 20.292371701326488 28.219174948317132 30.230264272236173 21.25818907812021 84-85 20.1694982359514 28.32054654712781 29.983327321197617 21.526627895723173 86-87 20.202470791221575 28.35792070627218 29.840905751483206 21.598702751023044 88-89 20.30916886560947 28.098265687429556 29.641866345924573 21.9506991010364 90-91 20.158173368606157 27.81914596411974 30.10393106048574 21.918749606788364 92-93 20.45054902028069 27.824961098506897 30.14726035382431 21.577229527388102 94-95 19.991702971664722 27.562059440333325 30.256745954758756 22.18949163324319 96-97 19.90691961467413 27.52347136429766 30.3933106006848 22.176298420343418 98-99 19.842384436959055 28.031890929185632 29.900935908401355 22.224788725453966 100-101 19.61810321163876 27.504627772264335 30.633168985831627 22.24410003026528 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2220.0 1 1401.5 2 700.0 3 872.0 4 769.0 5 706.0 6 1279.0 7 2900.0 8 4845.5 9 5469.5 10 4541.0 11 3573.0 12 3401.5 13 3528.5 14 3600.5 15 3650.5 16 3786.5 17 3918.0 18 4014.5 19 4211.0 20 4585.5 21 5012.5 22 5627.5 23 6489.5 24 7295.5 25 8470.0 26 10080.5 27 11784.0 28 13026.5 29 14529.5 30 16856.0 31 19024.5 32 22254.0 33 25492.5 34 28354.5 35 30610.5 36 33654.5 37 37193.5 38 37866.0 39 37264.5 40 36317.0 41 36425.5 42 36579.5 43 37024.0 44 38550.5 45 39624.5 46 38641.5 47 39610.5 48 44873.5 49 43292.0 50 36062.5 51 31258.0 52 28368.0 53 27392.0 54 26847.0 55 24824.0 56 22049.5 57 19657.5 58 18236.0 59 16467.5 60 13884.5 61 11674.0 62 9785.0 63 7421.5 64 5127.0 65 3871.0 66 2817.0 67 2013.0 68 1460.5 69 1015.5 70 771.5 71 620.0 72 516.0 73 470.5 74 376.0 75 266.0 76 198.5 77 134.5 78 89.0 79 51.0 80 27.5 81 19.5 82 13.0 83 13.5 84 11.5 85 7.0 86 5.5 87 6.0 88 7.0 89 6.5 90 5.5 91 4.0 92 3.0 93 3.5 94 4.5 95 4.5 96 3.0 97 3.0 98 6.5 99 4.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3399872823150402 2 0.2939647207203409 3 0.2892219376552383 4 0.2939647207203409 5 0.2925594516640142 6 0.21404004314176 7 0.2025344027430852 8 0.2063988926479836 9 0.20042649915859514 10-11 0.22128596171344458 12-13 0.20986815063079017 14-15 0.2053449408557386 16-17 0.20661846593803468 18-19 0.20047041381660535 20-21 0.19489325224930878 22-23 0.19704507049180903 24-25 0.1864616379113486 26-27 0.18769124833563447 28-29 0.20490579427563652 30-31 0.215708800146148 32-33 0.21008772392084119 34-35 0.2180362770206891 36-37 0.19340015387696166 38-39 0.18856954149583863 40-41 0.1859785766732363 42-43 0.19533239882941086 44-45 0.19897731544425826 46-47 0.20429098906349358 48-49 0.21193213955727 50-51 0.21597228809420924 52-53 0.23630477475293615 54-55 0.21035121186890243 56-57 0.18250931869042977 58-59 0.181718854846246 60-61 0.17921571933966407 62-63 0.18707644312349153 64-65 0.17965486591976615 66-67 0.17811785288940885 68-69 0.17429727764252062 70-71 0.1851441981710423 72-73 0.1845293929588994 74-75 0.18993089589415513 76-77 0.19296100729685958 78-79 0.19058961576430827 80-81 0.18997481055216534 82-83 0.1821140867683379 84-85 0.17407770435246958 86-87 0.17680041314910255 88-89 0.17925963399767428 90-91 0.18422199035282794 92-93 0.179830524551807 94-95 0.1774591330192557 96-97 0.17807393823139864 98-99 0.18760341901961405 100-101 0.1717941421359387 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1138572.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.59612452010671 #Duplication Level Percentage of deduplicated Percentage of total 1 77.54001931086754 39.23224472344133 2 12.347614860175602 12.494829179835296 3 3.995957044305845 6.065398205720884 4 1.849103313822961 3.7422984606691396 5 1.0045282266619555 2.5412617620075144 6 0.6305034924033602 1.9140619927201543 7 0.42881102899452217 1.5187323353021356 8 0.29737237908267483 1.2036711936725915 9 0.24634129962679369 1.1217523563325955 >10 1.4478400316195066 13.95962875521616 >50 0.13631844661704662 4.714107896103465 >100 0.07073612689439622 6.352327157947732 >500 0.0027739651016651214 1.0352705821294304 >1k 0.001560355369686631 1.4418524452178823 >5k 3.467456377081402E-4 1.4772912958516977 >10k+ 1.733728188540701E-4 1.185271657831966 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13512 1.1867497180678956 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9698 0.8517687067660191 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7143 0.6273648043338498 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2922 0.25663726141166304 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2269 0.1992847180503297 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2167 0.190326127816247 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1920 0.16863228675920364 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1669 0.14658712843807858 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1592 0.13982427110450635 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1478 0.12981172907817864 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1348 0.11839391799552423 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 8.782931602041856E-5 0.0 18-19 0.0 0.0 0.0 8.782931602041856E-5 0.0 20-21 0.0 0.0 0.0 1.3174397403062783E-4 0.0 22-23 0.0 0.0 0.0 2.6348794806125566E-4 0.0 24-25 0.0 0.0 0.0 8.343785021939763E-4 0.0 26-27 0.0 0.0 0.0 0.0011856957662756506 0.0 28-29 0.0 0.0 0.0 0.002854452770663603 0.0 30-31 0.0 0.0 0.0 0.007465491861735578 0.0 32-33 0.0 0.0 0.0 0.01220827492683818 0.0 34-35 0.0 0.0 0.0 0.015545788935614086 0.0 36-37 0.0 0.0 0.0 0.01787326581015518 0.0 38-39 0.0 0.0 0.0 0.01998116939464522 0.0 40-41 0.0 0.0 0.0 0.024021317931584477 0.0 42-43 0.0 0.0 0.0 0.028281039758574773 0.0 44-45 0.0 0.0 0.0 0.03276033487561612 0.0 46-47 0.0 0.0 0.0 0.03952319220918835 0.0 48-49 0.0 0.0 0.0 0.0473400013350056 0.0 50-51 0.0 0.0 0.0 0.060426569422047965 0.0 52-53 0.0 0.0 0.0 0.09059593947506175 0.0 54-55 0.0 0.0 0.0 0.12054573623802448 0.0 56-57 0.0 0.0 0.0 0.16112288023945784 0.0 58-59 0.0 0.0 0.0 0.2253261102503838 0.0 60-61 0.0 0.0 0.0 0.32672505559595705 0.0 62-63 0.0 0.0 0.0 0.391982237399128 0.0 64-65 0.0 0.0 0.0 0.45882034689066653 0.0 66-67 0.0 0.0 0.0 0.5950875306963459 0.0 68-69 0.0 0.0 0.0 0.762753694979325 0.0 70-71 0.0 0.0 0.0 0.9173772058332719 0.0 72-73 0.0 0.0 0.0 1.0615051134227786 0.0 74-75 0.0 0.0 0.0 1.3239391096917894 0.0 76-77 0.0 0.0 0.0 1.5079415267545664 0.0 78-79 0.0 0.0 0.0 1.6724458356608103 0.0 80-81 0.0 0.0 0.0 1.855569959563383 0.0 82-83 0.0 0.0 0.0 2.1627090776867863 0.0 84-85 0.0 0.0 0.0 2.4509209782077903 0.0 86-87 0.0 0.0 0.0 2.8349985771650807 0.0 88-89 0.0 0.0 0.0 3.3592078498329485 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 12095 0.0 32.052402 1 GGTATCA 7995 0.0 31.771053 1 CGTTCGC 65 0.005845102 29.229685 6 GTACATG 8480 0.0 25.971315 1 TCAACGC 15180 0.0 25.523832 4 ATCAACG 15180 0.0 25.49476 3 TATCAAC 15290 0.0 25.435724 2 CAACGCA 15260 0.0 25.387787 5 AACGCAG 15560 0.0 24.970154 6 TACATGG 8745 0.0 24.737164 2 GAGTACT 8520 0.0 24.028923 12-13 ACATGGG 8575 0.0 23.84145 3 ATGGGTA 540 0.0 22.887657 5 AGTACTT 8790 0.0 22.669384 12-13 GTACTTT 9095 0.0 22.509283 14-15 ACGCAGA 17275 0.0 22.326235 7 CGCAGAG 17315 0.0 22.275639 8 GTAAGGT 300 7.2759576E-12 22.185377 4 AGAGTAC 13680 0.0 21.84043 10-11 CATGGGT 930 0.0 21.469719 4 >>END_MODULE