FastQCFastQC Report
Thu 26 May 2016
SRR937470_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937470_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1138572
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139631.2263607395931044No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106990.9396858521024583No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77370.6795354180499784No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT42800.37590947256739143No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25740.22607265943655738No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23080.20271006137512604No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19930.17504382682869418No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT16440.1443913955375681No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT15490.13604761051562833No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC11780.10346293427205305No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA140300.029.575451
GGTATCA96400.029.0410981
GTACATG86700.025.0537011
TACATGG89650.024.0586052
TCAACGC173700.023.7128724
ATCAACG174000.023.6992913
TATCAAC174800.023.5908282
CAACGCA175000.023.5638685
ACATGGG87650.023.4151363
AACGCAG177850.023.2345836
GAGTACT99600.023.15359912-13
CTTACTA6250.022.7997369
GGACCGC1252.256732E-422.7987376
AGTACTT101300.022.5774812-13
CATGGGT8650.022.5181474
ACTATAC3200.022.269283
GTACTTT103600.022.05234714-15
AGAGTAC150850.021.4432410-11
CATGGGA60450.021.0621814
ACGCAGA197300.020.9209447
Produced by FastQC (version 0.11.5)