FastQCFastQC Report
Thu 26 May 2016
SRR937469_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937469_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1268636
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140071.1040992057611483No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101950.8036190049785754No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73150.5766035332435782No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT33240.2620136902941427No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT24140.19028310721120953No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23960.18886426051286578No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20000.15764963314930366No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT18840.14850595442664405No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18720.14756005662774824No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18650.14700828291172566No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC14310.11279831251832678No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA134450.033.3148461
GGTATCA86950.030.3508681
GTACATG94900.027.75821
TCAACGC166900.026.9144734
ATCAACG166500.026.8934843
TATCAAC166900.026.8575122
TACATGG97250.026.638962
CAACGCA168600.026.6388845
AACGCAG171300.026.258936
ACATGGG97750.025.4328673
GAGTACT94100.023.92522212-13
ACGCAGA187450.023.7440997
CGCAGAG187750.023.604968
CATGGGG26850.023.1919574
AGTACTT97300.022.9431112-13
CATGGGA66750.022.7169384
AGAGTAC150400.022.24804510-11
GTACTTT100850.022.205714-15
GCAGAGT193850.022.197939
ATGGGAG25350.020.9981865
Produced by FastQC (version 0.11.5)