##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937469_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1268636 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.70042864935253 33.0 31.0 34.0 30.0 34.0 2 31.763931498081405 33.0 31.0 34.0 30.0 34.0 3 31.785283564395147 33.0 31.0 34.0 30.0 34.0 4 35.440733196913854 37.0 35.0 37.0 33.0 37.0 5 35.44217017332001 37.0 35.0 37.0 33.0 37.0 6 35.53733458612242 37.0 35.0 37.0 33.0 37.0 7 35.48510683915639 37.0 35.0 37.0 33.0 37.0 8 35.48162120576745 37.0 35.0 37.0 33.0 37.0 9 37.067090954379346 39.0 37.0 39.0 33.0 39.0 10-11 36.98401078008192 39.0 37.0 39.0 33.0 39.0 12-13 36.87427993530059 39.0 37.0 39.0 33.0 39.0 14-15 37.957760539666225 40.0 38.0 41.0 33.0 41.0 16-17 37.930347239081975 40.0 38.0 41.0 33.0 41.0 18-19 37.97905348736754 40.0 38.0 41.0 33.0 41.0 20-21 37.95194011521035 40.0 38.0 41.0 33.0 41.0 22-23 37.815004855608706 40.0 37.5 41.0 33.0 41.0 24-25 37.66254977787166 40.0 37.0 41.0 32.0 41.0 26-27 37.48617609779322 40.0 37.0 41.0 32.0 41.0 28-29 37.29298120185774 39.5 37.0 41.0 32.0 41.0 30-31 37.19446673435091 39.0 36.5 41.0 32.0 41.0 32-33 36.948910483385305 39.0 35.5 41.0 31.0 41.0 34-35 36.70051102128585 39.0 35.0 41.0 31.0 41.0 36-37 36.63694629507597 39.0 35.0 41.0 30.5 41.0 38-39 36.567079524780944 39.0 35.0 41.0 30.0 41.0 40-41 36.36311597652912 39.0 35.0 41.0 30.0 41.0 42-43 36.03534741249658 39.0 35.0 41.0 30.0 41.0 44-45 35.65784511869441 39.0 35.0 41.0 28.0 41.0 46-47 35.14907901084314 39.0 35.0 41.0 25.0 41.0 48-49 34.69178393171879 39.0 35.0 40.0 23.0 41.0 50-51 33.713024066793 38.0 33.5 39.5 21.0 40.5 52-53 33.82953502817199 38.0 33.5 39.5 21.0 40.5 54-55 34.17187120655571 38.0 34.0 40.0 20.0 41.0 56-57 33.94739152916991 38.0 34.0 40.0 18.5 41.0 58-59 33.706267991764385 38.0 34.0 40.0 17.5 41.0 60-61 33.42560553224092 38.0 33.5 40.0 14.0 41.0 62-63 33.12582647820179 37.0 33.0 40.0 11.5 41.0 64-65 32.772960092571864 37.0 33.0 40.0 8.0 41.0 66-67 32.38007789468374 36.0 32.0 39.0 7.0 41.0 68-69 31.8940448639326 35.5 31.5 39.0 2.0 40.5 70-71 31.468119697060466 35.0 31.0 38.5 2.0 40.0 72-73 31.069221589171363 35.0 31.0 38.0 2.0 40.0 74-75 30.622997061410835 35.0 30.5 37.0 2.0 39.0 76-77 30.191021301618434 35.0 30.0 37.0 2.0 39.0 78-79 29.721945065408832 34.5 29.5 36.0 2.0 39.0 80-81 29.2685041256909 34.0 29.0 36.0 2.0 37.5 82-83 28.83227300817571 34.0 29.0 35.0 2.0 37.0 84-85 28.37049279698826 34.0 28.5 35.0 2.0 36.5 86-87 27.95024577577808 34.0 27.0 35.0 2.0 36.0 88-89 27.560834234563735 34.0 26.5 35.0 2.0 36.0 90-91 27.120999640558836 33.5 26.0 35.0 2.0 35.5 92-93 26.6879889109248 33.0 25.0 35.0 2.0 35.0 94-95 26.17416973820702 33.0 24.5 35.0 2.0 35.0 96-97 25.58472051873035 32.5 21.5 35.0 2.0 35.0 98-99 24.910778584243236 32.0 15.0 35.0 2.0 35.0 100-101 23.157095100564703 30.5 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.03521353518409143 1101 2 0.07060587229927862 1101 3 0.14653523751961472 1101 4 0.12627198586223898 1101 5 0.18195359854659898 1101 6 0.12289631832867798 1101 7 0.12444146821612634 1101 8 -0.08362135627598377 1101 9 0.05400206688309339 1101 10-11 0.06743033096844897 1101 12-13 0.1053074698592269 1101 14-15 0.007108040558783557 1101 16-17 -0.04679363539779757 1101 18-19 0.20533910956570622 1101 20-21 0.21896099047075523 1101 22-23 0.18507831527647056 1101 24-25 0.4054965837671247 1101 26-27 0.4609960901601724 1101 28-29 0.36328597521531236 1101 30-31 0.2697944067055573 1101 32-33 0.3153235196961077 1101 34-35 0.347887641910674 1101 36-37 0.3950764316502884 1101 38-39 0.44714237854427097 1101 40-41 0.5748534995351449 1101 42-43 0.5682750387220707 1101 44-45 0.5491420543778247 1101 46-47 0.7083489959481639 1101 48-49 0.8243862869533061 1101 50-51 0.8671234587863736 1101 52-53 0.808184516755297 1101 54-55 0.8886487275867481 1101 56-57 0.9746551191272559 1101 58-59 0.9881945131672509 1101 60-61 1.0580552883944776 1101 62-63 0.990108493540248 1101 64-65 0.9318511540354706 1101 66-67 1.006487614673759 1101 68-69 1.1169402257104473 1101 70-71 1.126083382079269 1101 72-73 1.3273255250037472 1101 74-75 1.273609415897436 1101 76-77 1.186510907791103 1101 78-79 1.0889782057851711 1101 80-81 1.35503901371861 1101 82-83 0.9709396865079682 1101 84-85 1.0192928075882683 1101 86-87 1.2619306868776867 1101 88-89 1.079342631735269 1101 90-91 1.3021368775963928 1101 92-93 1.1252508064465907 1101 94-95 0.9752887511560253 1101 96-97 0.9459484796976163 1101 98-99 1.045004994465824 1101 100-101 1.5378650106583756 1102 1 0.1557601647910971 1102 2 0.24888187834229214 1102 3 0.22436628724136298 1102 4 0.013829748494380567 1102 5 0.12904897773773172 1102 6 0.2116591541890145 1102 7 0.2237392400055711 1102 8 0.20103017192509043 1102 9 0.25503468423881515 1102 10-11 0.1588304306096191 1102 12-13 0.3739054742119805 1102 14-15 0.45928708606091107 1102 16-17 0.3966264671075521 1102 18-19 0.48131136736606095 1102 20-21 0.5369471798817713 1102 22-23 0.5182164171323365 1102 24-25 0.630339961562477 1102 26-27 0.7138206853931308 1102 28-29 0.6313792183740574 1102 30-31 0.6132024574701163 1102 32-33 0.5266157486933025 1102 34-35 0.6730143616789519 1102 36-37 0.8875070307680417 1102 38-39 0.9554663999328383 1102 40-41 1.0253344598017193 1102 42-43 0.9118666390117127 1102 44-45 0.9167405710556267 1102 46-47 1.0741006715939605 1102 48-49 0.9497359891391568 1102 50-51 0.8603843169510057 1102 52-53 0.7403705310882955 1102 54-55 0.918478803084092 1102 56-57 1.0002617906482385 1102 58-59 0.7634915709288776 1102 60-61 0.8625165503680563 1102 62-63 0.8898402792754254 1102 64-65 0.7792791411022861 1102 66-67 0.799006811735353 1102 68-69 0.8425563772618396 1102 70-71 0.7771300177778393 1102 72-73 0.7280733884671058 1102 74-75 0.381244067627339 1102 76-77 0.66448925096417 1102 78-79 0.8193621066764933 1102 80-81 0.7952255686150416 1102 82-83 0.7578782720470905 1102 84-85 0.5730087778088695 1102 86-87 0.6740465187301865 1102 88-89 0.765963333701265 1102 90-91 0.722811714570124 1102 92-93 0.4593830508538801 1102 94-95 0.5350356343438953 1102 96-97 0.6054623457548729 1102 98-99 0.8205261944359741 1102 100-101 0.891757571658335 1103 1 0.16868922465195269 1103 2 0.04108744692893751 1103 3 0.08425221269696692 1103 4 0.08994365181994368 1103 5 0.1425329700512279 1103 6 0.10087241896007271 1103 7 0.10053964930010295 1103 8 0.21388432354853393 1103 9 0.2192428149929242 1103 10-11 0.14939377622552286 1103 12-13 0.26843212804062944 1103 14-15 0.2705830444302748 1103 16-17 0.2355036894489544 1103 18-19 0.1095264977089414 1103 20-21 0.09842595002100296 1103 22-23 0.04088991760446703 1103 24-25 0.046020293971601234 1103 26-27 0.0165557023443057 1103 28-29 -0.1932868412131512 1103 30-31 -0.06273457069080024 1103 32-33 0.005930883571444667 1103 34-35 0.2173457699894925 1103 36-37 0.3996276422027023 1103 38-39 0.3676520337558884 1103 40-41 0.4598475893812477 1103 42-43 0.444418599594691 1103 44-45 0.40999296952055886 1103 46-47 0.4921637993644836 1103 48-49 0.6125523554170016 1103 50-51 0.4334071089287832 1103 52-53 0.47976961415032093 1103 54-55 0.28383048197132865 1103 56-57 0.4513737193519063 1103 58-59 0.5391864977047973 1103 60-61 0.4577982996129819 1103 62-63 0.5871009308955593 1103 64-65 0.3752900991073034 1103 66-67 0.743126795367651 1103 68-69 0.678915001212804 1103 70-71 0.687869973576877 1103 72-73 0.7278308284602595 1103 74-75 0.777955290257168 1103 76-77 0.6017283779315932 1103 78-79 0.6284498007818264 1103 80-81 0.46105864758310133 1103 82-83 0.558700326255174 1103 84-85 0.49620057194268696 1103 86-87 0.7166614073283881 1103 88-89 0.7818357267544691 1103 90-91 0.9295987066273028 1103 92-93 0.8600284846872306 1103 94-95 0.8238609050199734 1103 96-97 0.8621960612391213 1103 98-99 1.0010848570130868 1103 100-101 1.354426771276561 1104 1 0.07872718810095591 1104 2 0.09807057889100435 1104 3 -1.6988731456990536E-4 1104 4 -0.07097472301879293 1104 5 0.10897270176486984 1104 6 0.13955158461326533 1104 7 0.11895099807886567 1104 8 0.10613026037068352 1104 9 0.016566144608660238 1104 10-11 0.13971287197522742 1104 12-13 0.23961566765652265 1104 14-15 0.2093907693753252 1104 16-17 0.11627243805361331 1104 18-19 0.19059851438667863 1104 20-21 0.23279738049034648 1104 22-23 0.2736481637041095 1104 24-25 0.3971442388139721 1104 26-27 0.2886116870690216 1104 28-29 0.08209225636455386 1104 30-31 0.11873506079754037 1104 32-33 0.05452544682196958 1104 34-35 0.03170595276944255 1104 36-37 0.13357258945793404 1104 38-39 0.3472698420646978 1104 40-41 0.2697703788881327 1104 42-43 0.37345601814009655 1104 44-45 0.22180668940415416 1104 46-47 0.08233161069498607 1104 48-49 0.10357093558101127 1104 50-51 -0.04548099803813699 1104 52-53 0.13159144515628185 1104 54-55 0.24201699683082012 1104 56-57 0.12657196487217703 1104 58-59 -0.018670427890135954 1104 60-61 0.1602999115986634 1104 62-63 0.22026268535530136 1104 64-65 0.23605848810554875 1104 66-67 0.34351791728470715 1104 68-69 0.3813966350645579 1104 70-71 0.3126254287312129 1104 72-73 0.4139915755225978 1104 74-75 0.346509257939541 1104 76-77 0.3834188177452198 1104 78-79 0.4415406228770422 1104 80-81 0.6257821077559456 1104 82-83 0.5387708890242244 1104 84-85 0.4562939996860216 1104 86-87 0.3533503013338972 1104 88-89 0.3093900685273958 1104 90-91 0.48923543422894156 1104 92-93 0.6755803631562465 1104 94-95 0.557104120320826 1104 96-97 0.7922778367358383 1104 98-99 0.9380930515441754 1104 100-101 1.185559596399461 1105 1 0.18496493936564562 1105 2 0.16536807420732202 1105 3 0.247583611992372 1105 4 0.21827654904668492 1105 5 0.14580837434370864 1105 6 0.11568324892238735 1105 7 0.1348528410098524 1105 8 0.12928668740878635 1105 9 0.25993345614546115 1105 10-11 0.23355286791030494 1105 12-13 0.3367223572485045 1105 14-15 0.6356183473943346 1105 16-17 0.49707581849967397 1105 18-19 0.517091132227236 1105 20-21 0.40431382241133207 1105 22-23 0.44598851832531494 1105 24-25 0.44802849710951165 1105 26-27 0.46054336350735525 1105 28-29 0.47319481874396985 1105 30-31 0.4308031964109418 1105 32-33 0.4351269468020291 1105 34-35 0.4167867951054376 1105 36-37 0.5853261630700004 1105 38-39 0.5058571923822157 1105 40-41 0.7083148507781161 1105 42-43 0.6079209201693416 1105 44-45 0.7970955369152861 1105 46-47 0.6553448637787866 1105 48-49 0.7393878630532029 1105 50-51 0.7428176932628645 1105 52-53 0.6406542116781111 1105 54-55 0.6656206751530078 1105 56-57 0.6723275305172081 1105 58-59 0.8540126591162434 1105 60-61 0.9225546568823404 1105 62-63 1.01770391191436 1105 64-65 0.6652801255391552 1105 66-67 0.7103472903188361 1105 68-69 0.8908208195248442 1105 70-71 0.8258744148214028 1105 72-73 0.8303879299820807 1105 74-75 0.7986308058245477 1105 76-77 0.9441086206235596 1105 78-79 0.8088248416160297 1105 80-81 0.8179122116574575 1105 82-83 0.8443796692673651 1105 84-85 0.7056655477076212 1105 86-87 0.8289623761351166 1105 88-89 0.8530787497261798 1105 90-91 0.8437271516662257 1105 92-93 0.5985284080299849 1105 94-95 0.9431861695687331 1105 96-97 0.6907947938754404 1105 98-99 0.9013180250593145 1105 100-101 0.9516855840156389 1106 1 0.09530351719972785 1106 2 0.20650770067926416 1106 3 0.1343776732083768 1106 4 4.8485238053075363E-4 1106 5 0.14524838389108652 1106 6 0.03899275315379214 1106 7 0.05018280915466278 1106 8 0.008823823169095135 1106 9 0.12773636573519553 1106 10-11 0.04759243182593309 1106 12-13 -0.17725150286929647 1106 14-15 -0.00971360104457375 1106 16-17 -0.0924859332343928 1106 18-19 0.04652440090593757 1106 20-21 -0.06828697259150118 1106 22-23 -0.16274274264991107 1106 24-25 0.03237099356531559 1106 26-27 0.2439293516806771 1106 28-29 0.1906753731738675 1106 30-31 0.14127632932802925 1106 32-33 0.4738131724794883 1106 34-35 0.27378202010488195 1106 36-37 0.11628170087722367 1106 38-39 0.06282015563660792 1106 40-41 0.19723110532066812 1106 42-43 0.17560042202700998 1106 44-45 0.2941742752449912 1106 46-47 0.26469873708979463 1106 48-49 0.3225012001372889 1106 50-51 0.46048152325658265 1106 52-53 0.44736537594678083 1106 54-55 0.413546940016694 1106 56-57 0.270157010145752 1106 58-59 0.30934260103199307 1106 60-61 0.4178862118853388 1106 62-63 0.4366433267177783 1106 64-65 0.5276323083871901 1106 66-67 0.5554791110222155 1106 68-69 0.4291113142442775 1106 70-71 0.22448040580123063 1106 72-73 0.4508022704588974 1106 74-75 0.5644656178211953 1106 76-77 0.5551818088839546 1106 78-79 0.5118830189848076 1106 80-81 0.4284814740831422 1106 82-83 0.4018006574996953 1106 84-85 0.4874764807671248 1106 86-87 0.4667947803039638 1106 88-89 0.7047875074785068 1106 90-91 0.832420459190164 1106 92-93 0.7545352564378156 1106 94-95 0.882563596576535 1106 96-97 0.9703270772594621 1106 98-99 1.060703176525589 1106 100-101 1.2843202407362462 1107 1 0.10645884235355396 1107 2 0.049959445133076485 1107 3 0.04662669831263955 1107 4 0.11501657573484181 1107 5 0.15182365126032948 1107 6 0.26267694271145103 1107 7 0.22927677364566534 1107 8 0.3886140393114914 1107 9 0.2787109427491714 1107 10-11 0.31392303104219366 1107 12-13 0.38723145577792195 1107 14-15 0.4561606656920034 1107 16-17 0.2687991920956776 1107 18-19 0.46072122546294736 1107 20-21 0.3801479352894148 1107 22-23 0.2888351780931018 1107 24-25 0.3673673999569971 1107 26-27 0.30600554892594545 1107 28-29 0.46302905116424853 1107 30-31 0.4021181101641318 1107 32-33 0.38645382917754745 1107 34-35 0.24826182338868819 1107 36-37 0.25004918884143024 1107 38-39 0.3462211911695121 1107 40-41 0.387021433016308 1107 42-43 0.37650703123843954 1107 44-45 0.27229629219152685 1107 46-47 0.2156085335369866 1107 48-49 0.1257658857244479 1107 50-51 0.08641797296279918 1107 52-53 -0.1233820945832349 1107 54-55 -0.10143227123807463 1107 56-57 -0.144765707355198 1107 58-59 -0.08688020587091927 1107 60-61 -0.04513906448784866 1107 62-63 -0.0056673076536881695 1107 64-65 0.05938554303308052 1107 66-67 -0.051635914722673704 1107 68-69 -0.11977450781553856 1107 70-71 -0.003374680150550091 1107 72-73 -0.007297531454401707 1107 74-75 -0.07092417314394694 1107 76-77 -0.13295792909339355 1107 78-79 -0.24346874825773668 1107 80-81 -0.19285738804181918 1107 82-83 -0.11064509973174452 1107 84-85 -0.03781742221023521 1107 86-87 0.0320119983889775 1107 88-89 0.15087780505477255 1107 90-91 0.3095416841086127 1107 92-93 0.42844398675464035 1107 94-95 0.32364037230509624 1107 96-97 0.44317501583505603 1107 98-99 0.6535700632177601 1107 100-101 0.70126844656156 1108 1 0.0672516052692238 1108 2 -0.01800072035768352 1108 3 -0.07388591940316402 1108 4 -0.00511013324087628 1108 5 0.08744098251730037 1108 6 0.0459312306620987 1108 7 -0.009996190651641257 1108 8 0.017367785898997568 1108 9 0.09970928729612893 1108 10-11 0.041924187404774216 1108 12-13 0.13957418092088858 1108 14-15 0.33166930159324437 1108 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-1.7512636811482558 2316 100-101 -1.9068433543285401 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4879.0 3 890.0 4 731.0 5 869.0 6 1040.0 7 1226.0 8 1490.0 9 2090.0 10 2733.0 11 3704.0 12 5391.0 13 7413.0 14 8943.0 15 11367.0 16 15711.0 17 21137.0 18 16736.0 19 9597.0 20 8921.0 21 8853.0 22 9556.0 23 10625.0 24 11551.0 25 12935.0 26 14794.0 27 17317.0 28 20501.0 29 24605.0 30 29508.0 31 36566.0 32 46645.0 33 60677.0 34 82324.0 35 118229.0 36 168143.0 37 236268.0 38 204498.0 39 30169.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.16677739602513 19.723034237517638 15.657684933847118 20.45250343261011 2 14.799228046484822 21.345415992340552 40.969590692283546 22.885765268891078 3 18.431911423219937 27.606793479764804 30.832992700037398 23.128302396977865 4 11.525428017071663 17.410929595994894 38.96469442261379 32.098947964319656 5 12.026603338935644 39.13533614661087 33.847159659560745 14.990900854892747 6 28.648325406098007 37.96154323167355 18.138399180338876 15.251732181889565 7 26.431104506712234 35.27309900357317 21.238277389492843 17.057519100221754 8 26.536828991410427 33.37744117133565 20.228591832913718 19.85713800434021 9 27.3866542168589 16.398039945239805 21.15408770004811 35.06121813785318 10-11 23.966563134362495 27.823605602357738 27.041760926094682 21.168070337185082 12-13 26.019629018967766 25.820068116156136 28.02341482991897 20.136888034957128 14-15 22.817303359337636 26.116175365331273 28.315726158236693 22.7507951170944 16-17 19.882234999123103 29.924373492091327 28.506998592223574 21.686392916561992 18-19 19.869645991276556 27.141563995342864 33.091063763907826 19.897726249472754 20-21 21.33036110659098 25.799404712064216 32.992269788031365 19.877964393313437 22-23 20.593474247789636 26.12875986694777 33.230158084439665 20.047607800822927 24-25 20.39113889257483 26.28697262083041 33.381039940856446 19.940848545738316 26-27 20.020364861214023 26.46547215986021 33.4052449391977 20.108918039728067 28-29 19.644856734446734 26.773356315964303 33.49581537321563 20.08597157637334 30-31 19.883588645646526 26.407483192049106 33.38027445744488 20.32865370485949 32-33 19.693401350494053 26.59661120323853 33.18724180511456 20.522745641152856 34-35 20.345986758199896 26.600630136845627 32.95397678597031 20.099406318984165 36-37 20.353996631048464 26.163838472004723 32.98523467136925 20.49693022557757 38-39 19.9922598479613 26.712330930990223 32.940428472702145 20.354980748346332 40-41 19.976787157824816 26.794285960258573 32.92776019767643 20.301166684240187 42-43 19.97455199425643 26.906764098882324 32.19397825309155 20.924705653769692 44-45 20.05958068375589 26.94524968074723 32.056651418635674 20.93851821686121 46-47 20.635949374287616 27.36290054399813 31.390714196005202 20.610435885709048 48-49 20.605687648191953 27.671029759057607 30.698204060642233 21.025078532108207 50-51 20.515327332281018 27.92646103819153 30.611410531704614 20.94680109782284 52-53 20.74762544932073 28.2734587987853 30.002713997948106 20.976201753945865 54-55 20.327812382478328 28.091629770787314 30.216184571043925 21.364373275690436 56-57 20.734486925342956 28.261872230865826 29.786998791650543 21.216642052140674 58-59 21.106607378488057 27.724527917482984 29.97689435127693 21.19197035275203 60-61 20.67657415970186 27.95165556167162 30.69523560569146 20.67653467293506 62-63 20.53814485259102 28.0747947267459 30.79323887512547 20.59382154553761 64-65 20.408009350296542 28.209939428399945 30.459183270550515 20.922867950752998 66-67 20.450346848977937 28.221138239635803 29.99542694846353 21.33308796292274 68-69 20.412856534762856 28.16041211418589 30.31188129367716 21.114850057374092 70-71 20.631609873060423 28.231083255080385 30.16874204801322 20.96856482384597 72-73 20.57026315706328 28.15565729234794 30.175306395200046 21.098773155388734 74-75 20.15976836785346 29.005592169304396 29.713686144285944 21.1209533185562 76-77 20.35904399224944 28.398275476649186 30.01296236175653 21.229718169344842 78-79 20.375195146735734 28.36510379456484 29.83745703612342 21.422244022576002 80-81 20.452283887924512 28.674051387675743 29.573501561342518 21.30016316305723 82-83 20.44555094761228 28.4065683003956 29.856510463050252 21.29137028894187 84-85 20.33783930521033 28.43488715985572 29.658626728237973 21.56864680669598 86-87 20.39097144617183 28.48507943026603 29.466476597391416 21.657472526170725 88-89 20.410270019658903 28.261707473305677 29.279879124203354 22.048143382832066 90-91 20.28821309382445 28.065551656147214 29.712359509740775 21.933875740287558 92-93 20.57274399218691 27.943128418769582 29.87791850518194 21.60620908386157 94-95 20.125155298880742 27.72577673135051 29.901935618395605 22.247132351373146 96-97 20.085202098303064 27.682981536386226 29.99207844784965 22.23973791746106 98-99 19.959582986783314 28.123551104971284 29.657928472075767 22.25893743616963 100-101 19.777037130338197 27.62117002202068 30.36129792913976 22.240494918501362 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2671.0 1 1674.0 2 833.0 3 997.5 4 841.5 5 805.0 6 1444.0 7 3050.5 8 5057.5 9 5902.0 10 4919.5 11 3750.5 12 3604.5 13 3749.5 14 3838.5 15 3964.0 16 4021.0 17 4158.5 18 4379.5 19 4508.0 20 4772.0 21 5267.5 22 5990.0 23 6905.0 24 7820.0 25 9071.0 26 10937.0 27 12719.0 28 14014.5 29 15806.0 30 18346.0 31 20989.0 32 24656.5 33 28219.5 34 31204.5 35 33376.0 36 37157.5 37 41314.0 38 41786.5 39 41129.5 40 40248.0 41 40313.5 42 40465.0 43 41216.5 44 42922.5 45 44207.5 46 43285.5 47 44086.5 48 50078.0 49 48749.5 50 40856.5 51 35430.5 52 32162.5 53 31333.5 54 30870.5 55 28449.0 56 25185.5 57 22518.5 58 20737.5 59 18694.0 60 15986.0 61 13526.0 62 11195.5 63 8430.5 64 6024.5 65 4609.5 66 3281.0 67 2380.5 68 1745.5 69 1190.0 70 925.5 71 727.5 72 562.0 73 516.5 74 412.0 75 310.5 76 237.0 77 151.5 78 94.5 79 58.5 80 39.0 81 28.0 82 19.5 83 13.0 84 9.5 85 8.0 86 5.0 87 2.5 88 2.5 89 5.5 90 4.0 91 1.5 92 3.0 93 2.0 94 1.0 95 1.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3383161127384056 2 0.2989825292676544 3 0.30229317156378976 4 0.30347554381240954 5 0.290232974627868 6 0.21653177113056857 7 0.2217342090244956 8 0.22063066159245048 9 0.2170047200300165 10-11 0.2244536651963211 12-13 0.22520250095378028 14-15 0.20711220554989768 16-17 0.22149773457477165 18-19 0.20667866905873708 20-21 0.212354055852112 22-23 0.21022578580459644 24-25 0.2021068296974073 26-27 0.2151917492487995 28-29 0.21696530762172916 30-31 0.22591192430295215 32-33 0.22677899728527334 34-35 0.21444291349134031 36-37 0.20994989894658517 38-39 0.19792911441895072 40-41 0.19915089907585784 42-43 0.19903266185099586 44-45 0.2198818258349913 46-47 0.20801869094050615 48-49 0.21696530762172916 50-51 0.22528132577035492 52-53 0.23454324171787652 54-55 0.22953786586538613 56-57 0.19280550134159838 58-59 0.17964175697363152 60-61 0.18831248679684323 62-63 0.18894308532944043 64-65 0.18649951601562623 66-67 0.19887501221784656 68-69 0.18799718753054462 70-71 0.1925690268918744 72-73 0.20683631869188643 74-75 0.1963132056791704 76-77 0.20991048653829786 78-79 0.20506276031895676 80-81 0.19059840647750811 82-83 0.2038015632537623 84-85 0.18551420580844308 86-87 0.1916231290929786 88-89 0.2004515085493396 90-91 0.19623438086259576 92-93 0.20143681875652275 94-95 0.1993479611172945 96-97 0.19469729693939003 98-99 0.203762150845475 100-101 0.1833859357609275 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1268636.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.77088412812225 #Duplication Level Percentage of deduplicated Percentage of total 1 76.96182903618717 38.30458275248426 2 12.55754518973266 12.500002531436863 3 4.2104789308394235 6.286777769721271 4 1.9389858247645375 3.860201552417094 5 0.9856420053693143 2.452813702052309 6 0.6603782205054625 1.9720564736107762 7 0.43499790191982474 1.5155161120699516 8 0.31745422808482365 1.26399820815938 9 0.24479301687900246 1.096520839061247 >10 1.4639554232998537 14.021845746658764 >50 0.14277747240116873 4.885100049643121 >100 0.07593156212398545 6.698615339483618 >500 0.0030118960594735017 1.040023235782301 >1k 0.001743729297589922 1.6153418092403906 >5k 1.5852084523544746E-4 0.5771332096607139 >10k+ 3.170416904708949E-4 1.9094706685179221 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14007 1.1040992057611483 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10195 0.8036190049785754 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7315 0.5766035332435782 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3324 0.2620136902941427 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2414 0.19028310721120953 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2396 0.18886426051286578 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2000 0.15764963314930366 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1884 0.14850595442664405 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1872 0.14756005662774824 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1865 0.14700828291172566 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1431 0.11279831251832678 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 7.882481657465183E-5 0.0 5 0.0 0.0 0.0 7.882481657465183E-5 0.0 6 7.882481657465183E-5 0.0 0.0 7.882481657465183E-5 0.0 7 7.882481657465183E-5 0.0 0.0 7.882481657465183E-5 0.0 8 7.882481657465183E-5 0.0 0.0 7.882481657465183E-5 0.0 9 7.882481657465183E-5 0.0 0.0 7.882481657465183E-5 0.0 10-11 7.882481657465183E-5 0.0 0.0 7.882481657465183E-5 0.0 12-13 7.882481657465183E-5 0.0 0.0 7.882481657465183E-5 0.0 14-15 7.882481657465183E-5 0.0 0.0 7.882481657465183E-5 0.0 16-17 7.882481657465183E-5 0.0 0.0 7.882481657465183E-5 0.0 18-19 1.5764963314930365E-4 0.0 0.0 7.882481657465183E-5 0.0 20-21 1.5764963314930365E-4 0.0 0.0 7.882481657465183E-5 0.0 22-23 1.5764963314930365E-4 0.0 0.0 7.882481657465183E-5 0.0 24-25 1.5764963314930365E-4 0.0 0.0 3.152992662986073E-4 3.941240828732591E-5 26-27 1.5764963314930365E-4 0.0 0.0 8.670729823211701E-4 7.882481657465183E-5 28-29 1.5764963314930365E-4 0.0 0.0 0.002719456171825488 7.882481657465183E-5 30-31 1.5764963314930365E-4 0.0 0.0 0.007843069249177857 7.882481657465183E-5 32-33 1.5764963314930365E-4 0.0 0.0 0.0129272699182429 7.882481657465183E-5 34-35 1.5764963314930365E-4 0.0 0.0 0.01651379907238956 7.882481657465183E-5 36-37 1.5764963314930365E-4 0.0 0.0 0.019193842835927722 7.882481657465183E-5 38-39 1.5764963314930365E-4 0.0 0.0 0.02092798880057006 7.882481657465183E-5 40-41 1.5764963314930365E-4 0.0 0.0 0.024396280729854742 7.882481657465183E-5 42-43 1.5764963314930365E-4 0.0 0.0 0.028534583600023963 7.882481657465183E-5 44-45 1.5764963314930365E-4 0.0 0.0 0.03385525871881296 7.882481657465183E-5 46-47 2.364744497239555E-4 0.0 0.0 0.040555368127658364 7.882481657465183E-5 48-49 2.364744497239555E-4 0.0 0.0 0.04914727313429541 7.882481657465183E-5 50-51 2.364744497239555E-4 0.0 0.0 0.06148335692822843 7.882481657465183E-5 52-53 2.364744497239555E-4 0.0 0.0 0.08895380550449458 7.882481657465183E-5 54-55 3.152992662986073E-4 0.0 0.0 0.12020784527634404 7.882481657465183E-5 56-57 3.152992662986073E-4 0.0 0.0 0.16297030826809267 7.882481657465183E-5 58-59 3.152992662986073E-4 0.0 0.0 0.22677899728527334 7.882481657465183E-5 60-61 3.152992662986073E-4 0.0 0.0 0.3312612916549743 7.882481657465183E-5 62-63 3.152992662986073E-4 0.0 0.0 0.400272418566082 7.882481657465183E-5 64-65 3.152992662986073E-4 0.0 0.0 0.4666823265302261 7.882481657465183E-5 66-67 3.152992662986073E-4 0.0 0.0 0.5997386169082384 7.882481657465183E-5 68-69 3.152992662986073E-4 0.0 0.0 0.7611718412531254 7.882481657465183E-5 70-71 3.152992662986073E-4 0.0 0.0 0.9149196459819837 7.882481657465183E-5 72-73 3.152992662986073E-4 0.0 0.0 1.0505377428986722 7.882481657465183E-5 74-75 3.152992662986073E-4 0.0 0.0 1.2991118019668368 7.882481657465183E-5 76-77 3.152992662986073E-4 0.0 0.0 1.477492361875274 7.882481657465183E-5 78-79 3.152992662986073E-4 0.0 0.0 1.6407385570013777 7.882481657465183E-5 80-81 3.152992662986073E-4 0.0 0.0 1.812734306767268 7.882481657465183E-5 82-83 3.152992662986073E-4 0.0 0.0 2.1061596864664094 7.882481657465183E-5 84-85 3.152992662986073E-4 0.0 0.0 2.3771199934417755 7.882481657465183E-5 86-87 3.152992662986073E-4 0.0 0.0 2.745153062028825 7.882481657465183E-5 88-89 3.152992662986073E-4 0.0 0.0 3.2325663153181843 7.882481657465183E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 13445 0.0 33.314846 1 GGTATCA 8695 0.0 30.350868 1 GTACATG 9490 0.0 27.7582 1 TCAACGC 16690 0.0 26.914473 4 ATCAACG 16650 0.0 26.893484 3 TATCAAC 16690 0.0 26.857512 2 TACATGG 9725 0.0 26.63896 2 CAACGCA 16860 0.0 26.638884 5 AACGCAG 17130 0.0 26.25893 6 ACATGGG 9775 0.0 25.432867 3 GAGTACT 9410 0.0 23.925222 12-13 ACGCAGA 18745 0.0 23.744099 7 CGCAGAG 18775 0.0 23.60496 8 CATGGGG 2685 0.0 23.191957 4 AGTACTT 9730 0.0 22.94311 12-13 CATGGGA 6675 0.0 22.716938 4 AGAGTAC 15040 0.0 22.248045 10-11 GTACTTT 10085 0.0 22.2057 14-15 GCAGAGT 19385 0.0 22.19793 9 ATGGGAG 2535 0.0 20.998186 5 >>END_MODULE