FastQCFastQC Report
Thu 26 May 2016
SRR937469_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937469_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1268636
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149831.1810322267380082No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120600.950627287890301No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87870.6926336632414656No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT47250.3724472583152299No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27310.21527057406537414No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25310.1995056107504438No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23080.1819276766542964No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT18230.1436976406155903No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT17390.13707635602331955No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13480.10625585274263068No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGT851.6330778E-533.5271876
GGTATCA106200.029.6364631
GTATCAA152650.028.8407861
TAAGGTG6050.025.9114575
AGGTAAG4450.024.5528032
GTACATG96100.023.9450021
GGTAAGG4900.023.2674313
ATCAACG191150.023.1122343
GTAAGGT5150.023.0603584
TACATGG99650.023.025182
TATCAAC192500.023.024182
TCAACGC192000.023.009914
AGTACTT111750.022.97365412-13
GAGTACT109650.022.95879712-13
CAACGCA194350.022.7561285
AACGCAG198050.022.3754396
ACATGGG97850.022.2835873
TACCTGG9650.022.152282
GTCCTAC2154.3795808E-822.1133461
GTACTTT114850.021.58759714-15
Produced by FastQC (version 0.11.5)