##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937469_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1268636 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.11762712078169 33.0 31.0 34.0 31.0 34.0 2 32.318433341005615 34.0 31.0 34.0 31.0 34.0 3 32.373025044220725 34.0 31.0 34.0 30.0 34.0 4 36.01566643229421 37.0 35.0 37.0 35.0 37.0 5 35.92984512500039 37.0 35.0 37.0 35.0 37.0 6 36.03189330903427 37.0 35.0 37.0 35.0 37.0 7 35.98173865474415 37.0 35.0 37.0 35.0 37.0 8 35.96764083629977 37.0 35.0 37.0 35.0 37.0 9 37.627820746061126 39.0 37.0 39.0 35.0 39.0 10-11 37.56406920503596 39.0 37.0 39.0 35.0 39.0 12-13 37.48788974930555 39.0 37.0 39.0 35.0 39.0 14-15 38.59120701288628 40.0 38.0 41.0 34.0 41.0 16-17 38.60451776553716 40.0 38.0 41.0 34.5 41.0 18-19 38.69565777732935 40.0 38.0 41.0 34.5 41.0 20-21 38.629779542753006 40.0 38.0 41.0 34.0 41.0 22-23 38.50264339022383 40.0 38.0 41.0 34.0 41.0 24-25 38.355704867274774 40.0 38.0 41.0 34.0 41.0 26-27 38.261049268663356 40.0 38.0 41.0 34.0 41.0 28-29 38.14865217446139 40.0 38.0 41.0 34.0 41.0 30-31 38.09325409337272 40.0 37.0 41.0 34.0 41.0 32-33 37.899217742520314 40.0 36.5 41.0 33.5 41.0 34-35 37.729354992290936 40.0 36.0 41.0 33.0 41.0 36-37 37.519032251961946 40.0 36.0 41.0 33.0 41.0 38-39 37.35424463754773 40.0 36.0 41.0 33.0 41.0 40-41 37.13317768059554 40.0 35.5 41.0 32.5 41.0 42-43 36.82159185140576 40.0 35.0 41.0 31.5 41.0 44-45 36.36856277135443 39.0 35.0 41.0 30.5 41.0 46-47 35.79593200886622 39.0 35.0 41.0 29.0 41.0 48-49 35.307836132665315 39.0 35.0 41.0 26.5 41.0 50-51 35.012670695140294 39.0 35.0 41.0 24.5 41.0 52-53 34.76469176343727 39.0 35.0 40.0 24.0 41.0 54-55 34.52723003288571 38.0 34.5 40.0 23.0 41.0 56-57 34.27549036918391 38.0 34.0 40.0 22.5 41.0 58-59 34.01802487080613 38.0 34.0 40.0 22.0 41.0 60-61 33.686078985619204 37.5 34.0 40.0 20.5 41.0 62-63 33.33713610523428 37.0 33.0 40.0 18.5 41.0 64-65 32.98581153301656 36.5 33.0 39.5 17.5 41.0 66-67 32.55132480869217 36.0 32.5 39.0 13.5 40.5 68-69 32.146660665470634 35.5 32.0 39.0 9.0 40.0 70-71 31.737058935738858 35.0 31.5 38.0 7.5 40.0 72-73 31.300001340021883 35.0 31.0 37.5 4.5 40.0 74-75 30.854923319218436 35.0 31.0 37.0 2.0 39.0 76-77 29.55882223112067 33.5 29.5 35.5 2.0 38.5 78-79 30.16286941250288 34.0 30.5 36.0 2.0 38.5 80-81 30.00160605563771 34.0 30.5 36.0 2.0 37.5 82-83 29.66202362222103 34.0 30.0 35.0 2.0 37.0 84-85 29.280578511093807 34.0 30.0 35.0 2.0 36.5 86-87 28.90915282240138 34.0 29.0 35.0 2.0 36.0 88-89 28.501092117833643 34.0 29.0 35.0 2.0 36.0 90-91 28.054988192042476 34.0 29.0 35.0 2.0 35.0 92-93 27.69690833304431 34.0 27.5 35.0 2.0 35.0 94-95 27.274030139456865 33.0 27.0 35.0 2.0 35.0 96-97 26.79165063895396 33.0 25.5 35.0 2.0 35.0 98-99 26.319376874060012 33.0 25.0 35.0 2.0 35.0 100-101 24.47417383709748 31.0 20.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1473659947938657 1101 2 0.17738742775921423 1101 3 0.15909874596664508 1101 4 0.08422068600230403 1101 5 -0.046218293903578456 1101 6 0.051353884427769 1101 7 0.015991029977818982 1101 8 0.01970391420586992 1101 9 0.14953436915823204 1101 10-11 0.1239353194002959 1101 12-13 0.05051262680336066 1101 14-15 0.11342969352264021 1101 16-17 0.01057088701764286 1101 18-19 0.027720901675259313 1101 20-21 0.17520203472866314 1101 22-23 0.1038363096164332 1101 24-25 0.20688591313059135 1101 26-27 0.1448315934418858 1101 28-29 0.24731600502800433 1101 30-31 0.22294217504667557 1101 32-33 0.250077141430296 1101 34-35 0.13947211043472407 1101 36-37 0.22851698640268125 1101 38-39 0.06426385528418166 1101 40-41 0.09039925810201765 1101 42-43 0.08238769236282195 1101 44-45 0.19903009279779837 1101 46-47 0.23124766992986423 1101 48-49 0.17202593434769398 1101 50-51 0.11774740534821859 1101 52-53 0.09288691555976669 1101 54-55 0.08817887657956902 1101 56-57 0.16047151882923316 1101 58-59 0.021955580403577812 1101 60-61 -0.14734398062181953 1101 62-63 -0.10943366693045675 1101 64-65 -0.1427904493032628 1101 66-67 0.060837753523813376 1101 68-69 0.046911356867589404 1101 70-71 -0.15659677062548027 1101 72-73 -0.2028344862665179 1101 74-75 -0.3071776276555944 1101 76-77 -0.21742414765190077 1101 78-79 -0.16475631519880807 1101 80-81 -0.1978608798893653 1101 82-83 -0.10531061324810054 1101 84-85 -0.10819315474234514 1101 86-87 0.05743915456207205 1101 88-89 -0.02769031094192087 1101 90-91 -0.07000420521852391 1101 92-93 -0.07464378187532716 1101 94-95 -0.17269570392292266 1101 96-97 0.11165007029890361 1101 98-99 0.3715661059525317 1101 100-101 0.886053851490832 1102 1 0.1491354323860179 1102 2 0.15352397246966376 1102 3 0.16115561001292633 1102 4 0.21680143266563334 1102 5 0.07177782936908983 1102 6 0.041706440707883985 1102 7 0.08238754939473125 1102 8 0.12899252868933786 1102 9 0.15032554829248568 1102 10-11 0.20245896602509816 1102 12-13 0.19066554776209443 1102 14-15 0.22801426648761236 1102 16-17 0.11981058857822546 1102 18-19 0.18327332523494988 1102 20-21 0.24695325856024652 1102 22-23 0.2290372560058529 1102 24-25 0.3069554501622136 1102 26-27 0.23771799040505215 1102 28-29 0.09554777765249867 1102 30-31 0.13223643720784395 1102 32-33 0.07046056832009384 1102 34-35 0.02544852331223524 1102 36-37 -0.046671403989009264 1102 38-39 -0.016707706641597042 1102 40-41 -0.22437204120674892 1102 42-43 -0.16303920147495177 1102 44-45 -0.15977393076915547 1102 46-47 -0.10202305150045277 1102 48-49 -0.2003963041900363 1102 50-51 -0.47038914339628235 1102 52-53 -0.23690544151869375 1102 54-55 -0.12889289330177434 1102 56-57 -0.3043414321025324 1102 58-59 -0.2739030582237092 1102 60-61 -0.27623911333216 1102 62-63 -0.5055859847978894 1102 64-65 -0.3514587773284532 1102 66-67 -0.18781159427026495 1102 68-69 -0.2346571444202823 1102 70-71 -0.3811378463870625 1102 72-73 -0.2736987221458165 1102 74-75 -0.18230343976139096 1102 76-77 -0.30916361221171584 1102 78-79 -0.4235112241400891 1102 80-81 -0.16419643464259082 1102 82-83 -0.04805778487094159 1102 84-85 -0.08644001473439644 1102 86-87 -0.1712204397329664 1102 88-89 -0.009969713659664592 1102 90-91 0.04198823496147597 1102 92-93 -0.012080119437019476 1102 94-95 -0.08272365992751318 1102 96-97 -0.14164650942714374 1102 98-99 -0.194569460499455 1102 100-101 0.40472774651260934 1103 1 0.11898206413967216 1103 2 0.0543072476514439 1103 3 0.05766707734299814 1103 4 0.11756993003186977 1103 5 0.1262503862012565 1103 6 0.025317843270357798 1103 7 0.09404622669870832 1103 8 0.08142630751436286 1103 9 0.14283472455156954 1103 10-11 -0.034703061511578426 1103 12-13 0.10359007236231577 1103 14-15 -0.14197790127015253 1103 16-17 0.061660393995161655 1103 18-19 0.10941456751927348 1103 20-21 0.03463299374178774 1103 22-23 0.11638877080837773 1103 24-25 -0.017246382185000186 1103 26-27 0.11619160512497473 1103 28-29 -0.10386279133926024 1103 30-31 0.013723707138915131 1103 32-33 0.018572345678549596 1103 34-35 0.14146176423496826 1103 36-37 0.2686548213849065 1103 38-39 0.3316194191599138 1103 40-41 0.27438636749769074 1103 42-43 0.27925266445866725 1103 44-45 0.2866748327608306 1103 46-47 0.27016132875618837 1103 48-49 0.25276642860514187 1103 50-51 0.12941334345528333 1103 52-53 0.19461874747224073 1103 54-55 0.019352292060439424 1103 56-57 0.0629588801170371 1103 58-59 0.16393355111351582 1103 60-61 0.13996888900783944 1103 62-63 0.05813340792561661 1103 64-65 -0.0031968777472712873 1103 66-67 0.20563627902372872 1103 68-69 0.4849806869913422 1103 70-71 0.4869146056856799 1103 72-73 0.5470797527938132 1103 74-75 0.37520697732461983 1103 76-77 0.39446398479827494 1103 78-79 0.28924396707720135 1103 80-81 0.17290694025377107 1103 82-83 0.11193066804974094 1103 84-85 0.3279992359590125 1103 86-87 0.16150564428349767 1103 88-89 0.21742621612227353 1103 90-91 0.0950194451983215 1103 92-93 0.214218175355942 1103 94-95 0.3425467103994464 1103 96-97 0.2565603300516379 1103 98-99 0.38170623442731966 1103 100-101 0.6851304266987661 1104 1 0.09456229158073626 1104 2 0.12187679278429187 1104 3 0.1187567147348716 1104 4 0.06470379201992671 1104 5 0.01662087570049664 1104 6 -0.02330543854768763 1104 7 0.006657873251555202 1104 8 -0.01473644235590399 1104 9 0.04780523432861372 1104 10-11 -0.020042857105302403 1104 12-13 -0.013531737816443012 1104 14-15 -0.060439395165083454 1104 16-17 0.019709808910533866 1104 18-19 0.054020446384853926 1104 20-21 -0.054994908519184094 1104 22-23 0.03883040843599872 1104 24-25 0.04067896851865527 1104 26-27 -0.010991942482171169 1104 28-29 -0.06265214639015682 1104 30-31 -0.02903724100334415 1104 32-33 -0.0897688249102444 1104 34-35 -0.14116015607326204 1104 36-37 -0.21728531713348787 1104 38-39 -0.3788574638915918 1104 40-41 -0.2564408920667205 1104 42-43 -0.3939492660284145 1104 44-45 -0.5266777028889038 1104 46-47 -0.6231644262050082 1104 48-49 -0.6955269143310119 1104 50-51 -0.7060005460426595 1104 52-53 -0.3601935105113938 1104 54-55 -0.45259091609822377 1104 56-57 -0.3639556119713703 1104 58-59 -0.2151265675865659 1104 60-61 -0.18532208002655892 1104 62-63 -0.2119155235189183 1104 64-65 -0.45488950017202967 1104 66-67 -0.3172561651960777 1104 68-69 -0.28985675343011863 1104 70-71 -0.22070365612676923 1104 72-73 -0.3330469485315888 1104 74-75 -0.4472036442500702 1104 76-77 -0.2593067131727942 1104 78-79 -0.1932574351380758 1104 80-81 -0.25332579801419186 1104 82-83 -0.2048423738558931 1104 84-85 -0.2084820852349374 1104 86-87 0.07687227550562525 1104 88-89 0.14424656125452628 1104 90-91 0.02019983526139768 1104 92-93 -0.07453653261618243 1104 94-95 0.042412207612745334 1104 96-97 0.14404997263782704 1104 98-99 0.31495604251237097 1104 100-101 0.7959034356404793 1105 1 0.08307559356386207 1105 2 0.11108615894519147 1105 3 0.06274704195606517 1105 4 0.13632901445723178 1105 5 0.07498700436528338 1105 6 0.09843531196684552 1105 7 0.038498165314408084 1105 8 0.029194823218801957 1105 9 0.04164282627437643 1105 10-11 0.0473510709640621 1105 12-13 0.02846786771076637 1105 14-15 0.09880386178149081 1105 16-17 0.09840521854757611 1105 18-19 0.20685242774737844 1105 20-21 0.27206838360212515 1105 22-23 0.2275558624758176 1105 24-25 0.12197942839088682 1105 26-27 0.16715404632507358 1105 28-29 0.2412121025188938 1105 30-31 0.18170411105487716 1105 32-33 0.12155986544555475 1105 34-35 0.12919958453629476 1105 36-37 0.04167650915533727 1105 38-39 0.17088326039760915 1105 40-41 0.03797731793376613 1105 42-43 -0.13703328927936553 1105 44-45 0.06697543751335644 1105 46-47 -0.004595575677967645 1105 48-49 0.08310200314713256 1105 50-51 0.08303300108181588 1105 52-53 0.12308182657981348 1105 54-55 0.07009044987035651 1105 56-57 0.014412090213689055 1105 58-59 -0.04017706502112617 1105 60-61 -0.04073851380675819 1105 62-63 0.10760279351372759 1105 64-65 -0.1297723466702081 1105 66-67 -0.05579337048201438 1105 68-69 -0.26848392255240583 1105 70-71 -0.3625313764182394 1105 72-73 -0.3269271566515002 1105 74-75 -0.2135626549210521 1105 76-77 -0.05414234313546373 1105 78-79 -0.21043694375173416 1105 80-81 -0.3119246765531791 1105 82-83 -0.17082865691142857 1105 84-85 0.00210328069867316 1105 86-87 -0.08472907641054306 1105 88-89 -0.18265309568527144 1105 90-91 -0.132036066024245 1105 92-93 -0.11124736165921689 1105 94-95 -0.10141792650253834 1105 96-97 -0.31017725133912677 1105 98-99 -0.14531612185930598 1105 100-101 0.17093095858628615 1106 1 -0.06725036809187657 1106 2 -0.03294745097358742 1106 3 -0.05777877203006199 1106 4 0.15088679904582136 1106 5 -0.07055477656172826 1106 6 -0.13645248666740883 1106 7 -0.05595071621286252 1106 8 -0.0570496080236822 1106 9 -0.11578596510360484 1106 10-11 -0.1512143961165009 1106 12-13 -0.25239258975028633 1106 14-15 -0.2293731660199967 1106 16-17 -0.08362413701750171 1106 18-19 -0.044328207861454416 1106 20-21 0.04594961579095269 1106 22-23 0.04706779210849987 1106 24-25 -0.0042669438021150086 1106 26-27 -0.2539318892589719 1106 28-29 -0.019837217295979315 1106 30-31 0.1174081580678461 1106 32-33 0.10355820514599401 1106 34-35 -0.05767427859784391 1106 36-37 0.08010109994920356 1106 38-39 0.11261769640066888 1106 40-41 0.06310049616155311 1106 42-43 -0.18137023828875698 1106 44-45 -0.13701124861527347 1106 46-47 0.23971632457816838 1106 48-49 0.39569700152749476 1106 50-51 0.3234970980873726 1106 52-53 0.42414554614918387 1106 54-55 0.3757839265717351 1106 56-57 0.38525003002586544 1106 58-59 0.4072867814969072 1106 60-61 0.42611746110776494 1106 62-63 0.3436076222838622 1106 64-65 0.2110690553780188 1106 66-67 0.18689003294849016 1106 68-69 0.28678005327488876 1106 70-71 0.1545089887952038 1106 72-73 0.20321724744390934 1106 74-75 0.19169535379486646 1106 76-77 0.18692994022233123 1106 78-79 0.24377367298529506 1106 80-81 0.2933430369153136 1106 82-83 0.18150730326236442 1106 84-85 0.10832015831256214 1106 86-87 0.127408618291053 1106 88-89 0.26145638179249175 1106 90-91 0.3049892699372414 1106 92-93 0.18491808076602823 1106 94-95 -0.27171436735010346 1106 96-97 0.09960461467496273 1106 98-99 0.5281356750280928 1106 100-101 0.6493591952196276 1107 1 0.23876490195464584 1107 2 0.10983672695066105 1107 3 0.0893559827041841 1107 4 0.19366029973028276 1107 5 0.08472177191692509 1107 6 0.07711143254891795 1107 7 0.08610836557639345 1107 8 0.130876983562338 1107 9 0.16438241656150154 1107 10-11 0.13328822777533844 1107 12-13 0.2089322963458713 1107 14-15 0.12326140305775368 1107 16-17 0.22488554341430245 1107 18-19 0.14701111641269193 1107 20-21 0.2650218752064504 1107 22-23 0.19945192413654667 1107 24-25 0.264568424315641 1107 26-27 0.2364777183444957 1107 28-29 0.15300976510888376 1107 30-31 0.1944173516493919 1107 32-33 0.22735362923161517 1107 34-35 0.2066762504036106 1107 36-37 0.3819414895569011 1107 38-39 0.21688612220898307 1107 40-41 0.24013829518402474 1107 42-43 0.16850936388047444 1107 44-45 -0.03690097025803851 1107 46-47 -0.11766124971231307 1107 48-49 0.06652128443928262 1107 50-51 0.2151199269822115 1107 52-53 0.2185409514301213 1107 54-55 -0.038079035039601195 1107 56-57 0.14403723901132537 1107 58-59 0.29149131916445015 1107 60-61 0.2807615823310883 1107 62-63 0.06892009090950779 1107 64-65 0.2631668142731911 1107 66-67 0.11958079299265734 1107 68-69 0.04605212050007168 1107 70-71 0.004998946612140287 1107 72-73 -0.018454999541365424 1107 74-75 0.06499874836031339 1107 76-77 -0.05356731636449297 1107 78-79 -0.010822719382549195 1107 80-81 0.023561856586294283 1107 82-83 -0.009311306369777839 1107 84-85 -0.10539395938886642 1107 86-87 -0.2369541815680698 1107 88-89 -0.02803426606098469 1107 90-91 0.004581941207337792 1107 92-93 0.10265791627278986 1107 94-95 0.07647958550748868 1107 96-97 0.11004201497424404 1107 98-99 0.3698603361054076 1107 100-101 0.6858996579743852 1108 1 0.09608571348118033 1108 2 0.11784399239860477 1108 3 0.0858916676717385 1108 4 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0.4403753393402674 2316 76-77 0.3181916200530033 2316 78-79 -0.03977699553982106 2316 80-81 -0.06496267781288978 2316 82-83 -0.04954375243945819 2316 84-85 -0.11257654294188768 2316 86-87 -0.27264145656310745 2316 88-89 -0.3496080960351904 2316 90-91 -0.07233559713208848 2316 92-93 -0.2243483484322617 2316 94-95 0.007582003513558533 2316 96-97 0.040787826692927354 2316 98-99 -0.13086024671058638 2316 100-101 -0.37858825199527857 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 6.0 4 10.0 5 30.0 6 74.0 7 170.0 8 394.0 9 826.0 10 1470.0 11 2479.0 12 4000.0 13 5702.0 14 7286.0 15 10973.0 16 15550.0 17 22401.0 18 19412.0 19 9985.0 20 7409.0 21 7351.0 22 7415.0 23 7916.0 24 8826.0 25 10128.0 26 12062.0 27 14599.0 28 18134.0 29 22279.0 30 27899.0 31 34570.0 32 44612.0 33 57542.0 34 80126.0 35 116406.0 36 172321.0 37 264478.0 38 229628.0 39 24165.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.95245152126266 20.583282791517178 15.9879078211916 19.476357866028565 2 15.532430106035145 22.511579365554816 39.687901021254326 22.268089507155718 3 19.14008431102381 27.842974659397967 30.886164352895552 22.13077667668267 4 12.31456461900813 18.369492904190015 38.12921909830716 31.186723378494698 5 12.158134987898235 39.717441521274665 33.3138338549838 14.8105896358433 6 28.862967785873963 38.65758184380705 17.288095245602364 15.191355124716624 7 26.7087643737053 35.69889235367749 20.67346346785051 16.918879804766696 8 27.12637825191781 33.72701074224601 19.59852944422198 19.548081561614204 9 27.654626945202477 16.712542782236643 20.81175500577787 34.82107526678301 10-11 24.446768024870806 28.2126236367248 26.43216809234485 20.90844024605955 12-13 26.569246516518874 26.21330141349344 27.60661503735039 19.610837032637303 14-15 23.00809688515855 26.607198597548866 28.160283958519226 22.224420558773357 16-17 19.984968107479215 30.241022641640313 28.394236014112796 21.379773236767676 18-19 19.89897023259627 27.272480049438926 33.27944343373513 19.549106284229676 20-21 21.406258375136762 25.77082788128352 33.53073694897512 19.2921767946046 22-23 20.878669580588394 26.047464868390712 33.5939966380858 19.479868912935096 24-25 20.791065017698205 26.26680797378908 33.73599819963481 19.206128808877914 26-27 20.29576302382831 26.523135036027423 33.82246178318594 19.358640156958327 28-29 19.854806676260036 26.77724660832605 33.954576932005715 19.4133697834082 30-31 20.061585829189777 26.327252261483984 33.7789562963687 19.832205612957537 32-33 19.98209100167424 26.70159131539701 33.54145712402927 19.77486055889948 34-35 20.6025999577499 26.451519584813926 33.46369644247838 19.4821840149578 36-37 20.696677376331746 26.17039087650043 33.31897407924732 19.813957667920505 38-39 20.219984298096538 26.741319022950634 33.32417651714125 19.71452016181158 40-41 20.24662708609877 26.836933525455684 33.21863008774778 19.69780930069776 42-43 20.24766837744298 26.99983444100187 32.50806114645665 20.244436035098506 44-45 20.407863248402222 27.051297614130455 32.18870503438339 20.35213410308394 46-47 21.134588644812226 27.57930564795576 31.339131161341786 19.94697454589023 48-49 21.054857342846965 27.929957844488097 30.71432625276281 20.30085855990213 50-51 20.883571016430245 28.225629731459616 30.487704905110686 20.403094346999453 52-53 21.230163734908988 28.565916464612386 29.86494944176265 20.338970358715976 54-55 20.718787737380936 28.528474676739428 29.967500528126273 20.785237057753367 56-57 21.20348153449847 28.56926651931681 29.65716722527187 20.570084720912853 58-59 21.5497983661192 27.967990818485365 29.77946392818744 20.702746887207994 60-61 21.018164390731464 28.38808767841997 30.469732846931663 20.1240150839169 62-63 20.865993082334096 28.3705885691404 30.69308296469594 20.070335383829562 64-65 20.782044652682092 28.54274196853944 30.284021579081788 20.39119179969668 66-67 20.94923208863693 28.522444578271468 29.79030234046645 20.73802099262515 68-69 20.894724727975557 28.53312534091733 29.943892495562164 20.628257435544946 70-71 21.005867719345815 28.691799696682107 29.784114592365345 20.518217991606733 72-73 21.054235042295446 28.561789418631278 29.838870187693782 20.545105351379494 74-75 20.67626174883891 29.491832172506534 29.2559094964986 20.575996582155952 76-77 20.858977673658956 28.699445703889847 29.793770632395738 20.647805990055463 78-79 20.86887018813907 28.767235046144044 29.393931750320817 20.96996301539606 80-81 20.93579245741095 29.33296075470032 29.095540407177474 20.63570638071125 82-83 20.96349491558423 28.970311709901576 29.365343679389955 20.700849695124234 84-85 20.616609209015653 29.1223778993714 29.14618337865177 21.11482951296118 86-87 20.650091909736126 29.080721341661437 28.984515653032076 21.28467109557036 88-89 20.72741905479586 28.922795821654123 28.726443203566664 21.623341919983353 90-91 20.79321839619611 28.742233386848383 28.981691230639882 21.482856986315625 92-93 20.806931623759827 28.837426164877517 29.140077745866478 21.215564465496183 94-95 20.521292159453143 28.478854454705683 29.100033421722227 21.899819964118944 96-97 20.53745124685095 28.628503368972662 29.25283532865219 21.5812100555242 98-99 20.47240556677278 28.97505152193165 28.769830869332342 21.78271204196323 100-101 19.928629092021406 28.979284856091404 29.21551917152038 21.876566880366813 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2124.0 1 1415.0 2 868.5 3 1078.5 4 938.5 5 834.0 6 1412.5 7 3101.5 8 4914.0 9 5634.0 10 4951.0 11 4066.0 12 3948.0 13 4002.5 14 4196.5 15 4411.5 16 4525.0 17 4609.5 18 4663.0 19 4914.0 20 5302.0 21 5668.0 22 6283.5 23 7236.0 24 8118.0 25 9194.5 26 10916.0 27 12809.0 28 14131.5 29 15974.0 30 18531.5 31 20729.0 32 24095.5 33 27605.0 34 30713.5 35 33060.5 36 36756.0 37 40677.5 38 41819.5 39 41542.5 40 40305.0 41 40286.5 42 40464.5 43 41128.0 44 42732.0 45 44022.5 46 42756.5 47 43638.0 48 50379.5 49 48850.0 50 40777.5 51 35592.0 52 32182.0 53 31090.5 54 30560.0 55 28272.5 56 24819.5 57 21939.5 58 20564.0 59 19228.5 60 16391.0 61 13460.5 62 11097.5 63 8458.5 64 5870.0 65 4454.0 66 3270.5 67 2370.0 68 1719.5 69 1130.0 70 846.5 71 688.5 72 579.0 73 550.5 74 445.0 75 338.0 76 238.5 77 149.0 78 101.0 79 69.5 80 47.0 81 25.0 82 19.5 83 10.0 84 4.0 85 2.5 86 1.5 87 1.0 88 0.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05588679495142814 2 0.0 3 0.0 4 0.0 5 0.017814408545871314 6 0.0 7 0.0 8 0.0 9 1.5764963314930368E-4 10-11 0.0 12-13 0.0032712298878480512 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0010641350237577997 24-25 3.5471167458593324E-4 26-27 0.0014976715149183847 28-29 0.00405947805359457 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.01623791221437828 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 3.941240828732592E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.5471167458593324E-4 84-85 0.0016159087397803627 86-87 0.0 88-89 0.0 90-91 0.01146901081161184 92-93 0.0012217846569071032 94-95 0.0 96-97 0.0 98-99 1.1823722486197774E-4 100-101 0.01513436478233315 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1268636.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.40628864525641 #Duplication Level Percentage of deduplicated Percentage of total 1 75.4366008756034 35.76169275525858 2 13.12741810434507 12.446443436430943 3 4.539167943218069 6.455553171764719 4 2.1165244556371454 4.013462770747148 5 1.1596643888528109 2.748769237479061 6 0.6910141883245385 1.9655050841808388 7 0.4826645291400037 1.6016933791066452 8 0.34981129464553645 1.3266604164269713 9 0.2686510021184245 1.1462172256137082 >10 1.5874435216603497 14.443128164980957 >50 0.15095402175904152 4.994738580582339 >100 0.0838901954060283 7.172826808741677 >500 0.00351635311030756 1.1637849476000022 >1k 0.002176790020666585 1.9153514085424224 >5k 1.674453862051219E-4 0.6975089239861688 >10k+ 3.348907724102438E-4 2.146663688557865 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14983 1.1810322267380082 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12060 0.950627287890301 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8787 0.6926336632414656 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4725 0.3724472583152299 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2731 0.21527057406537414 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2531 0.1995056107504438 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2308 0.1819276766542964 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1823 0.1436976406155903 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1739 0.13707635602331955 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1348 0.10625585274263068 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 2.364744497239555E-4 0.0 26-27 0.0 0.0 0.0 7.882481657465184E-4 0.0 28-29 0.0 0.0 0.0 0.002719456171825488 0.0 30-31 0.0 0.0 0.0 0.007803656840890532 0.0 32-33 0.0 0.0 0.0 0.012848445101668248 0.0 34-35 0.0 0.0 0.0 0.01659262388896421 0.0 36-37 0.0 0.0 0.0 0.01923325524421505 0.0 38-39 0.0 0.0 0.0 0.020888576392282733 0.0 40-41 0.0 0.0 0.0 0.024396280729854742 0.0 42-43 0.0 0.0 0.0 0.02845575878344931 0.0 44-45 0.0 0.0 0.0 0.03357937186080168 0.0 46-47 0.0 0.0 0.0 0.040358306086221735 0.0 48-49 0.0 0.0 0.0 0.049383747584019366 0.0 50-51 0.0 0.0 0.0 0.06191689341938901 0.0 52-53 0.0 0.0 0.0 0.08993911571167773 0.0 54-55 0.0 0.0 0.0 0.12202081605756103 0.0 56-57 0.0 0.0 0.0 0.16533505276533222 0.0 58-59 0.0 0.0 0.0 0.22882844251621426 0.0 60-61 0.0 0.0 0.0 0.3338230981936505 0.0 62-63 0.0 0.0 0.0 0.4036224732705047 0.0 64-65 0.0 0.0 0.0 0.4702294432760855 0.0 66-67 0.0 0.0 3.941240828732591E-5 0.6039163321866949 0.0 68-69 0.0 0.0 7.882481657465183E-5 0.7673595893542355 0.0 70-71 3.941240828732591E-5 0.0 7.882481657465183E-5 0.9220532918819898 0.0 72-73 7.882481657465183E-5 0.0 7.882481657465183E-5 1.0583019873312756 0.0 74-75 7.882481657465183E-5 0.0 7.882481657465183E-5 1.308334305506071 0.0 76-77 7.882481657465183E-5 0.0 7.882481657465183E-5 1.4884884237874378 0.0 78-79 7.882481657465183E-5 0.0 7.882481657465183E-5 1.653350527653322 0.0 80-81 7.882481657465183E-5 0.0 7.882481657465183E-5 1.8277898467330267 0.0 82-83 7.882481657465183E-5 0.0 7.882481657465183E-5 2.124604693544878 0.0 84-85 7.882481657465183E-5 0.0 7.882481657465183E-5 2.3985603435500806 0.0 86-87 7.882481657465183E-5 0.0 7.882481657465183E-5 2.7714805507647586 0.0 88-89 7.882481657465183E-5 0.0 7.882481657465183E-5 3.2657909755044 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACCGT 85 1.6330778E-5 33.527187 6 GGTATCA 10620 0.0 29.636463 1 GTATCAA 15265 0.0 28.840786 1 TAAGGTG 605 0.0 25.911457 5 AGGTAAG 445 0.0 24.552803 2 GTACATG 9610 0.0 23.945002 1 GGTAAGG 490 0.0 23.267431 3 ATCAACG 19115 0.0 23.112234 3 GTAAGGT 515 0.0 23.060358 4 TACATGG 9965 0.0 23.02518 2 TATCAAC 19250 0.0 23.02418 2 TCAACGC 19200 0.0 23.00991 4 AGTACTT 11175 0.0 22.973654 12-13 GAGTACT 10965 0.0 22.958797 12-13 CAACGCA 19435 0.0 22.756128 5 AACGCAG 19805 0.0 22.375439 6 ACATGGG 9785 0.0 22.283587 3 TACCTGG 965 0.0 22.15228 2 GTCCTAC 215 4.3795808E-8 22.113346 1 GTACTTT 11485 0.0 21.587597 14-15 >>END_MODULE