FastQCFastQC Report
Thu 26 May 2016
SRR937468_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937468_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1316260
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143911.0933250269703554No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104460.793612204275751No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74680.5673651102365794No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT35730.2714509291477368No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT24670.18742497682828618No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24330.1848419005363682No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19910.15126190874143408No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19460.14784313129624846No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT17880.13583942382204123No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17090.12983757008493763No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC13640.10362694300518134No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTATC550.002573355334.5629651
GTATCAA147050.030.8963051
GGTATCA98250.029.264191
TCAACGC178700.025.2892864
ATCAACG178650.025.2678383
TATCAAC179050.025.2379282
CAACGCA179900.025.0913165
AACGCAG182500.024.8110126
TACCTGG9450.024.6383612
GTACATG100600.024.3289241
GAGTACT96150.023.71457712-13
TACATGG102200.023.5724432
AGTACTT96950.023.02891512-13
ACATGGG102200.022.7355523
GTACTTT102100.022.30974614-15
CGCAGAG202650.022.2250678
ACGCAGA203150.022.171217
ATACCGT1100.0028729421.5969166
AGAGTAC156500.021.55141310-11
GCAGAGT208600.021.0892689
Produced by FastQC (version 0.11.5)