##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937468_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1316260 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.835622901250513 33.0 31.0 34.0 30.0 34.0 2 31.899609499642928 33.0 31.0 34.0 30.0 34.0 3 31.920913801224682 33.0 31.0 34.0 30.0 34.0 4 35.55622749304849 37.0 35.0 37.0 33.0 37.0 5 35.589353927035695 37.0 35.0 37.0 33.0 37.0 6 35.641565496178565 37.0 35.0 37.0 33.0 37.0 7 35.544115904152676 37.0 35.0 37.0 33.0 37.0 8 35.557629951529336 37.0 35.0 37.0 33.0 37.0 9 37.17136204093416 39.0 37.0 39.0 34.0 39.0 10-11 37.07006366523331 39.0 37.0 39.0 33.0 39.0 12-13 36.9257939160956 39.0 37.0 39.0 33.0 39.0 14-15 38.04302037591357 40.0 38.0 41.0 33.0 41.0 16-17 38.01098719098051 40.0 38.0 41.0 33.0 41.0 18-19 38.06167284579034 40.0 38.0 41.0 33.0 41.0 20-21 38.00096751401699 40.0 38.0 41.0 33.0 41.0 22-23 37.84780932338596 40.0 38.0 41.0 33.0 41.0 24-25 37.76504414021546 40.0 37.5 41.0 33.0 41.0 26-27 37.76786843024934 40.0 37.0 41.0 33.0 41.0 28-29 37.67873824320423 40.0 37.0 41.0 33.0 41.0 30-31 37.39340593803656 39.5 37.0 41.0 32.5 41.0 32-33 37.07924650145108 39.0 36.0 41.0 32.0 41.0 34-35 37.0225757069272 39.0 36.0 41.0 31.5 41.0 36-37 36.854755519426256 39.0 35.5 41.0 31.0 41.0 38-39 36.71172222813122 39.0 35.0 41.0 31.0 41.0 40-41 36.455719234801634 39.0 35.0 41.0 30.5 41.0 42-43 36.10968501663805 39.0 35.0 41.0 30.0 41.0 44-45 35.736539513469985 39.0 35.0 41.0 28.5 41.0 46-47 35.20787078540714 39.0 35.0 41.0 25.0 41.0 48-49 34.735500964854964 39.0 35.0 40.0 23.5 41.0 50-51 33.70714828377372 38.0 33.5 39.5 21.0 40.5 52-53 33.80765350310729 38.0 33.5 39.5 21.0 40.5 54-55 34.165490860468296 38.0 34.0 40.0 20.5 41.0 56-57 33.9441185632018 38.0 34.0 40.0 18.5 41.0 58-59 33.688025162201995 38.0 34.0 40.0 17.5 41.0 60-61 33.38304286387188 38.0 33.0 40.0 13.5 41.0 62-63 33.07922750824305 37.0 33.0 40.0 10.5 41.0 64-65 32.66724013492775 36.5 32.5 39.5 7.5 41.0 66-67 32.30572873140565 36.0 32.0 39.0 6.0 41.0 68-69 31.820864039019646 35.5 31.5 39.0 2.0 40.5 70-71 31.355308221779893 35.0 31.0 38.5 2.0 40.0 72-73 30.91599532007354 35.0 31.0 37.5 2.0 40.0 74-75 30.432865087444732 35.0 30.0 37.0 2.0 39.0 76-77 29.96425250330482 35.0 30.0 36.5 2.0 39.0 78-79 29.470113047574188 34.0 29.0 36.0 2.0 38.5 80-81 28.96678315834258 34.0 29.0 35.5 2.0 37.5 82-83 28.49344772309422 34.0 28.5 35.0 2.0 37.0 84-85 27.981909729080883 34.0 27.0 35.0 2.0 36.5 86-87 27.539786592314588 34.0 26.0 35.0 2.0 36.0 88-89 27.07118426450701 33.0 25.5 35.0 2.0 36.0 90-91 26.551691535107047 33.0 25.0 35.0 2.0 35.0 92-93 26.058712944251134 33.0 24.0 35.0 2.0 35.0 94-95 25.54400384422531 32.0 21.5 35.0 2.0 35.0 96-97 24.84664807864708 32.0 16.5 34.5 2.0 35.0 98-99 23.977706912008266 32.0 2.0 34.0 2.0 35.0 100-101 21.491935104006807 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.02711303232004525 1101 2 0.008620246491368277 1101 3 -0.05502311671387616 1101 4 -0.03509346252054968 1101 5 -0.016476879979229864 1101 6 -0.12230785445804315 1101 7 -0.09541436201550368 1101 8 -0.13021072365886255 1101 9 -0.0542609338820057 1101 10-11 -0.23186849733187387 1101 12-13 -0.04602023312310877 1101 14-15 -0.06274604207290935 1101 16-17 -0.07770442668952882 1101 18-19 0.08653772796139236 1101 20-21 0.19166979034685738 1101 22-23 0.10151319901110867 1101 24-25 0.2343546385481048 1101 26-27 0.2323399326432778 1101 28-29 0.2288291169342287 1101 30-31 0.16774958721977384 1101 32-33 -0.008856679248268051 1101 34-35 -7.137188764119173E-4 1101 36-37 0.12010811562502255 1101 38-39 0.09808158782865917 1101 40-41 0.18563194897875945 1101 42-43 0.2418314745238419 1101 44-45 0.3943652303838405 1101 46-47 0.29382138390350576 1101 48-49 0.5006477018865496 1101 50-51 0.30477915397612065 1101 52-53 0.48008747662213835 1101 54-55 0.40473826753061104 1101 56-57 0.44986366020985 1101 58-59 0.3835404675855969 1101 60-61 0.3900788924173568 1101 62-63 0.34761865364977496 1101 64-65 0.13474586270248068 1101 66-67 0.06129222470754314 1101 68-69 0.15772759476895715 1101 70-71 0.08619375845477606 1101 72-73 0.05619511125536292 1101 74-75 0.15052829369114917 1101 76-77 0.012955582230482321 1101 78-79 0.029774369433695824 1101 80-81 0.17371710779741178 1101 82-83 0.18654792019973954 1101 84-85 0.374925956012202 1101 86-87 0.3451282181412836 1101 88-89 0.3620697847965175 1101 90-91 0.3581083221599535 1101 92-93 0.5415648011531076 1101 94-95 0.462723615630491 1101 96-97 0.5185201517768441 1101 98-99 0.8310514687444126 1101 100-101 1.052573785814495 1102 1 -0.07227320483732669 1102 2 0.06138528760963524 1102 3 -0.13815689185975444 1102 4 0.022694875855883367 1102 5 0.044035350307822796 1102 6 0.03914926064216928 1102 7 0.05741441396573066 1102 8 0.042286754791945214 1102 9 -0.050319272939105986 1102 10-11 -0.13223667445291198 1102 12-13 -0.038935762772190685 1102 14-15 0.0782404250974551 1102 16-17 0.03390707528748038 1102 18-19 0.06954279581116651 1102 20-21 -0.05170766236249591 1102 22-23 0.15270590724776412 1102 24-25 -0.036926927225650275 1102 26-27 -0.09616873344173626 1102 28-29 -0.057250693965052335 1102 30-31 0.0530819057455858 1102 32-33 -0.10901075673253757 1102 34-35 0.02174199158167056 1102 36-37 -0.04466878141533215 1102 38-39 0.17343909090711662 1102 40-41 0.18439128489861645 1102 42-43 -8.170493643504528E-4 1102 44-45 0.005164886339329655 1102 46-47 0.07981394610687431 1102 48-49 -0.1229601863856189 1102 50-51 -0.061875136989002044 1102 52-53 -0.03827187662370335 1102 54-55 0.033532812321361405 1102 56-57 0.001168368545506837 1102 58-59 0.14380085234616757 1102 60-61 0.1799902205157693 1102 62-63 0.010849206781756493 1102 64-65 0.007592335936926986 1102 66-67 -0.0887600626314935 1102 68-69 -0.12369090814242867 1102 70-71 -0.03086119584794389 1102 72-73 -0.13086052371094326 1102 74-75 -0.22902890370747642 1102 76-77 -0.2190739709448124 1102 78-79 -0.22009290116720592 1102 80-81 -0.09000971096662269 1102 82-83 -0.10859709208314783 1102 84-85 -0.07794489735429977 1102 86-87 -0.08776082750649294 1102 88-89 0.015323666125915736 1102 90-91 0.06657330201849376 1102 92-93 0.19575304794934922 1102 94-95 -0.016775415970862184 1102 96-97 0.2826194791574643 1102 98-99 0.4334696808268106 1102 100-101 0.46964359095907326 1103 1 -0.03659359318683997 1103 2 0.0036717997716557704 1103 3 -0.0359476320205232 1103 4 -0.1638198960409767 1103 5 -0.05375277997717376 1103 6 -0.10011501537829304 1103 7 -0.04196574053891311 1103 8 -0.12317675914479054 1103 9 -0.0351558947159063 1103 10-11 -0.0384762349498331 1103 12-13 -0.04643785067280959 1103 14-15 -0.2818950274550076 1103 16-17 -0.13534271592245517 1103 18-19 -0.15644308476091595 1103 20-21 -0.18425346568225365 1103 22-23 -0.2911276087768755 1103 24-25 -0.08079241422846906 1103 26-27 -0.25365124727407107 1103 28-29 -0.3715841316934174 1103 30-31 -0.30098884798653813 1103 32-33 -0.19282234980070712 1103 34-35 -0.09482158499208282 1103 36-37 -0.2078630083701256 1103 38-39 -0.35688831800821674 1103 40-41 0.029612602363904728 1103 42-43 -0.046607972216428095 1103 44-45 -0.08580885831042195 1103 46-47 0.22713621356694347 1103 48-49 0.3033356169341488 1103 50-51 0.14370021012447864 1103 52-53 -0.014440448499676961 1103 54-55 0.07293084447503873 1103 56-57 0.05874940518816629 1103 58-59 -0.028315887397020845 1103 60-61 0.027776393393942556 1103 62-63 0.15349588180001916 1103 64-65 0.20701490092283592 1103 66-67 0.2555184372119115 1103 68-69 0.3815706014097202 1103 70-71 0.33061181803659423 1103 72-73 0.3818397842543959 1103 74-75 0.2723588977331737 1103 76-77 0.337290863589061 1103 78-79 0.11968421544204233 1103 80-81 0.08896708723777991 1103 82-83 0.1660107740824266 1103 84-85 -0.03098866497095898 1103 86-87 0.11256214462003555 1103 88-89 0.2658404215283596 1103 90-91 0.2647881469918758 1103 92-93 0.359312005042991 1103 94-95 0.1540624846450669 1103 96-97 0.14111788713324458 1103 98-99 0.2479597111013696 1103 100-101 0.5995384129655505 1104 1 0.06346387807752762 1104 2 0.051870724502840915 1104 3 0.002173428417002299 1104 4 0.0807017947265436 1104 5 0.05517473207325452 1104 6 0.08509725751751773 1104 7 0.05226766695088969 1104 8 0.05468938842658133 1104 9 0.026649815332291382 1104 10-11 0.14318347954775845 1104 12-13 0.033175608726523365 1104 14-15 0.1642386395824431 1104 16-17 0.008684443884739323 1104 18-19 0.06141981846489131 1104 20-21 0.04182319356876718 1104 22-23 -0.1036438305047227 1104 24-25 -0.01471065672012628 1104 26-27 -0.011610090433137543 1104 28-29 -0.12176581504528627 1104 30-31 -0.15043221098245851 1104 32-33 -0.11523104464683342 1104 34-35 -0.11900122904102517 1104 36-37 -0.15700557418399086 1104 38-39 -0.19716686824922647 1104 40-41 -0.08550604798833916 1104 42-43 0.0889106595966993 1104 44-45 0.018418987444121626 1104 46-47 -0.09143256655912069 1104 48-49 -0.1007439158800878 1104 50-51 -0.13475316399491533 1104 52-53 -0.08785674438399838 1104 54-55 0.04919289490143797 1104 56-57 0.01608857834352051 1104 58-59 -0.06230226114563919 1104 60-61 0.003445192059118085 1104 62-63 0.2628701865831218 1104 64-65 0.31442081490133234 1104 66-67 0.20995994629608106 1104 68-69 0.24094027141342877 1104 70-71 0.19094552215227623 1104 72-73 0.22281540531483301 1104 74-75 0.05702287622779778 1104 76-77 -0.1086476792362987 1104 78-79 0.1306983051713715 1104 80-81 0.2341483071117132 1104 82-83 0.2695827985540582 1104 84-85 0.28305610699789696 1104 86-87 0.46348122137198544 1104 88-89 0.32379370295216603 1104 90-91 0.29708328974308174 1104 92-93 0.40744928720835816 1104 94-95 0.6085197519791414 1104 96-97 0.7732466413045422 1104 98-99 0.7396319395032975 1104 100-101 1.14456714859773 1105 1 0.09173974014649389 1105 2 0.002397040292311914 1105 3 0.07785400917925145 1105 4 0.15978225449665473 1105 5 0.16734654090568313 1105 6 0.11748685219386346 1105 7 0.16895248243788785 1105 8 0.09719392563166451 1105 9 -0.015987813221848057 1105 10-11 0.08047325571230601 1105 12-13 0.17269768979125644 1105 14-15 0.16984662506338566 1105 16-17 0.07607706336447251 1105 18-19 0.11352508162278951 1105 20-21 0.06141786991479847 1105 22-23 0.3148861150488216 1105 24-25 0.16433350540933844 1105 26-27 0.23951513642711575 1105 28-29 0.037980101470132865 1105 30-31 0.12812193299818375 1105 32-33 0.085228725468113 1105 34-35 0.13975255196320546 1105 36-37 0.30468226610638993 1105 38-39 0.1725125025916654 1105 40-41 0.04563127808547307 1105 42-43 0.3247424805283359 1105 44-45 0.43304088702064547 1105 46-47 0.46410282364051625 1105 48-49 0.5333879655476181 1105 50-51 0.5062631699433737 1105 52-53 0.509777859366757 1105 54-55 0.5492473413624168 1105 56-57 0.5853686751372251 1105 58-59 0.6548483740630715 1105 60-61 0.8188353916961475 1105 62-63 0.7789500413925552 1105 64-65 0.8798594113925589 1105 66-67 0.8911275204037636 1105 68-69 0.7203595091629751 1105 70-71 1.014545038183737 1105 72-73 1.0309279280008639 1105 74-75 0.9431632271049928 1105 76-77 0.8340685942059274 1105 78-79 0.7471290371737922 1105 80-81 0.7630170306446864 1105 82-83 0.821990542050731 1105 84-85 0.819057316919249 1105 86-87 0.6750359703133029 1105 88-89 0.7892020514095179 1105 90-91 1.0840524367485251 1105 92-93 1.2193367645223354 1105 94-95 1.1985439504062434 1105 96-97 1.2397136709476193 1105 98-99 1.0397650308523616 1105 100-101 1.360459465597124 1106 1 0.025748817302318372 1106 2 0.14212791058453078 1106 3 0.14662540817598568 1106 4 0.37671619297370995 1106 5 0.11502761954782414 1106 6 0.05329278154068362 1106 7 -0.265272110815296 1106 8 0.0037915846809539744 1106 9 -0.16560747508452067 1106 10-11 -0.5691416286999313 1106 12-13 -0.11394004675889136 1106 14-15 -0.1480791652065463 1106 16-17 0.023235542011924792 1106 18-19 -0.23050151285129772 1106 20-21 -0.06126095270908394 1106 22-23 -0.1231336317463061 1106 24-25 -0.12860913864406598 1106 26-27 -0.16968145565824244 1106 28-29 0.15835990877963724 1106 30-31 -0.04686864480790831 1106 32-33 -0.009244295207629705 1106 34-35 0.20340995568431453 1106 36-37 0.023731553470028643 1106 38-39 0.1540590694627042 1106 40-41 0.014900858996902855 1106 42-43 0.2679340828321344 1106 44-45 0.2118390928225935 1106 46-47 0.1583905854049874 1106 48-49 0.4078235628616227 1106 50-51 0.47203592255107196 1106 52-53 0.5191947449999006 1106 54-55 0.16512121667096125 1106 56-57 0.23184318213088773 1106 58-59 0.17356085117112485 1106 60-61 0.21490275374726053 1106 62-63 0.16403404489602025 1106 64-65 0.2167808754157008 1106 66-67 0.5012599619463529 1106 68-69 0.4071753384487451 1106 70-71 0.5627625183440479 1106 72-73 0.4270430645563188 1106 74-75 0.5229684132928476 1106 76-77 0.6630451581574093 1106 78-79 0.5234204085674818 1106 80-81 0.4070879492422357 1106 82-83 0.5539753072921272 1106 84-85 0.1840279886818621 1106 86-87 0.15458821704207537 1106 88-89 0.620752334075334 1106 90-91 0.7438358589955207 1106 92-93 0.9059447747142251 1106 94-95 1.0674883992742537 1106 96-97 1.393733364659962 1106 98-99 1.2758581380530423 1106 100-101 1.1199304956685232 1107 1 0.11012132886215653 1107 2 0.2039271461806429 1107 3 0.13737809779723165 1107 4 0.014362803865623164 1107 5 0.006385340577466536 1107 6 0.1421123357245193 1107 7 0.19253236756272685 1107 8 -0.07437955149098485 1107 9 0.16591817669726794 1107 10-11 0.12289266727015047 1107 12-13 0.17288914839321734 1107 14-15 0.26047062381304187 1107 16-17 0.10200446538846819 1107 18-19 0.09729955120430844 1107 20-21 -0.001383677383287818 1107 22-23 0.026147788162560914 1107 24-25 0.07459021622869955 1107 26-27 0.139597190113669 1107 28-29 0.07622102672313957 1107 30-31 0.12051907284110541 1107 32-33 0.2570475821867504 1107 34-35 0.009063691727995149 1107 36-37 0.09617134515143988 1107 38-39 -0.1158546290458915 1107 40-41 0.2146923494721804 1107 42-43 0.34288884867587655 1107 44-45 0.307108796214564 1107 46-47 0.3166062425441183 1107 48-49 0.4112741062892269 1107 50-51 0.35873376737236384 1107 52-53 0.41391961687858014 1107 54-55 0.43722530408660276 1107 56-57 0.42663308337385786 1107 58-59 0.306199134036504 1107 60-61 0.6271392247754974 1107 62-63 0.6388351662313809 1107 64-65 0.684999879489304 1107 66-67 0.6319605606882206 1107 68-69 0.6738210025400662 1107 70-71 0.7821947861616927 1107 72-73 0.742064566925567 1107 74-75 0.7702596987964689 1107 76-77 0.6580986024112967 1107 78-79 1.0058021118426481 1107 80-81 0.9290171087910544 1107 82-83 1.0351996226587126 1107 84-85 0.9191237413321751 1107 86-87 1.0549273468615752 1107 88-89 1.1158296448921092 1107 90-91 1.20441503589673 1107 92-93 1.0170048768968094 1107 94-95 1.3538590756357998 1107 96-97 1.301507911560286 1107 98-99 1.5333968833120188 1107 100-101 1.7256983981177356 1108 1 -0.007596053211440079 1108 2 0.012239728192877664 1108 3 -0.057745136285888066 1108 4 -0.07234566678255305 1108 5 -0.07751136030491068 1108 6 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1176.0 4 791.0 5 911.0 6 998.0 7 1258.0 8 1548.0 9 2007.0 10 2725.0 11 3754.0 12 5449.0 13 7692.0 14 8824.0 15 11980.0 16 15909.0 17 22192.0 18 18138.0 19 10329.0 20 9628.0 21 9735.0 22 10254.0 23 11185.0 24 12546.0 25 13805.0 26 15936.0 27 18619.0 28 21755.0 29 26446.0 30 31673.0 31 39311.0 32 49373.0 33 64454.0 34 87980.0 35 125996.0 36 178807.0 37 245609.0 38 197929.0 39 24452.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.27205246660853 19.753967558756433 15.579109332094285 20.39487064254076 2 14.82094339392758 21.341272113650763 41.05318546225449 22.784599030167165 3 18.46898739333599 27.58116058668157 30.797127657225438 23.152724362757006 4 11.61341806812592 17.418375754031526 38.85442032660359 32.113785851238966 5 12.042412243965583 39.08809868270768 33.867052463260485 15.002436610066244 6 28.588935467557075 37.99377281119188 18.158622706694533 15.258669014556512 7 26.583762276090354 35.2461463701465 21.143209248406983 17.02688210535616 8 26.567616802073907 33.31566489677673 20.195936868848413 19.92078143230095 9 27.43910054312233 16.44513794332398 21.125341545659744 34.99041996789395 10-11 24.03330022908949 27.79404641499318 27.03118907194385 21.141464283973484 12-13 26.133339730788975 25.818188880308174 27.95604338693487 20.09242800196798 14-15 22.852176892764 26.1397323383274 28.276761407984164 22.73132936092444 16-17 19.969474753267583 30.02559383524059 28.38676584325423 21.618165568237597 18-19 19.94864275300431 27.224595430316846 32.93203503471983 19.894726781959015 20-21 21.385260057506276 25.80818702941105 32.90277236287434 19.90378055020834 22-23 20.74258319138454 26.143084384211285 33.11603809430984 19.99829433009434 24-25 20.503972488579738 26.31291613669061 33.25872742549466 19.92438394923499 26-27 20.10787188329185 26.464781916572527 33.318522461315645 20.108823738819982 28-29 19.760021172362453 26.841072098367707 33.322328274433424 20.07657845483642 30-31 19.982224347921452 26.356136027149375 33.294771186079245 20.366868438849927 32-33 19.802145335861322 26.66243727792377 33.03980207679235 20.49561530942256 34-35 20.433066907957574 26.6580371655021 32.8538362055177 20.055059721022623 36-37 20.44113934918373 26.233905967484695 32.86683467116337 20.45812001216821 38-39 20.044811464424953 26.73946150097466 32.84143518518518 20.374291849415204 40-41 20.075853042154705 26.811484403374937 32.815197105980296 20.29746544849006 42-43 19.99157060173111 26.890998818970484 32.1653882967666 20.952042282531806 44-45 20.148764654742696 27.005701007643868 31.97476644375705 20.870767893856385 46-47 20.78777100335505 27.39543545236091 31.233981621469294 20.58281192281474 48-49 20.635895100082678 27.6719784871461 30.668689177573587 21.02343723519763 50-51 20.54125178757429 27.95585286573627 30.474852090724035 21.028043255965404 52-53 20.833861867915495 28.227860686283584 30.014059972200037 20.924217473600887 54-55 20.428256586827146 28.120438310631123 30.124201722212085 21.327103380329643 56-57 20.82640588100252 28.304171090908607 29.696755572831616 21.172667455257255 58-59 21.16379881327054 27.66564955699392 29.930507656491002 21.240043973244536 60-61 20.812388519539486 27.994413701276898 30.51700288754852 20.67619489163509 62-63 20.634520027316725 28.100063876066727 30.63477888146079 20.630637215155758 64-65 20.470732252284897 28.21816567896432 30.312305277866702 20.998796790884082 66-67 20.560014829989925 28.174202136264615 29.91729692220612 21.348486111539337 68-69 20.536930021937497 28.082812049749357 30.231186874502527 21.149071053810626 70-71 20.72118711748221 28.19719969760206 30.022283957149632 21.0593292277661 72-73 20.631525413018412 28.17056035898789 30.013845000706148 21.184069227287555 74-75 20.25135028543671 28.97677524276206 29.61849392352519 21.153380548276036 76-77 20.43949583927288 28.352377949973807 29.93777946318701 21.27034674756631 78-79 20.466112148337206 28.366056716808853 29.73962410114122 21.428207033712724 80-81 20.5471786031893 28.73962990485704 29.37717756770256 21.336013924251098 82-83 20.59299261147971 28.349321047175906 29.72146393885142 21.336222402492965 84-85 20.414209742931863 28.500683307130377 29.42857251620713 21.656534433730627 86-87 20.468595876030413 28.48084375277197 29.350362293046782 21.700198078150834 88-89 20.525993404144433 28.221170922350037 29.109285400759326 22.143550272746204 90-91 20.386216664350535 28.056523783506982 29.53784592071974 22.019413631422747 92-93 20.67594910205286 28.009897880521162 29.634977587010937 21.679175430415047 94-95 20.211930492950053 27.710353538525844 29.83049595740072 22.24722001112338 96-97 20.188385950204616 27.67718623376198 29.866646564855564 22.26778125117784 98-99 20.10879435643078 28.034554982194415 29.521025364154973 22.33562529721983 100-101 20.027239088472605 27.341292151404566 30.28867876336975 22.342789996753083 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2674.0 1 1704.0 2 858.5 3 993.5 4 855.5 5 816.5 6 1402.5 7 2950.0 8 4888.0 9 5669.0 10 4727.0 11 3696.0 12 3595.0 13 3758.5 14 3944.0 15 3975.0 16 4026.0 17 4267.5 18 4463.0 19 4646.0 20 5004.0 21 5519.5 22 6222.0 23 7190.0 24 8148.5 25 9418.5 26 11202.5 27 13034.0 28 14342.5 29 16194.0 30 18977.5 31 21682.5 32 25435.0 33 29130.0 34 32234.0 35 34728.5 36 38705.5 37 42987.0 38 43452.0 39 42690.5 40 42082.5 41 42131.5 42 41951.5 43 42894.0 44 44749.5 45 45995.5 46 45099.5 47 46015.5 48 52059.5 49 50614.5 50 42693.0 51 37029.5 52 33522.5 53 32538.5 54 31919.0 55 29522.0 56 26307.0 57 23284.5 58 21447.5 59 19500.0 60 16719.5 61 14264.0 62 11787.5 63 8829.0 64 6200.5 65 4735.5 66 3461.0 67 2487.5 68 1788.5 69 1234.0 70 974.0 71 800.0 72 639.0 73 568.5 74 457.0 75 333.0 76 245.0 77 157.5 78 104.0 79 71.0 80 48.0 81 34.0 82 26.5 83 19.0 84 13.0 85 8.0 86 3.5 87 3.5 88 3.5 89 4.0 90 4.0 91 3.5 92 3.0 93 1.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2602069499946819 2 0.23126130095877714 3 0.22085302295898984 4 0.2268548766960935 5 0.22487958306109737 6 0.2513181286371994 7 0.25390120492911733 8 0.2418974974549101 9 0.2364274535426132 10-11 0.24584808472490238 12-13 0.24600003038913282 14-15 0.23057754546974002 16-17 0.2467977451263428 18-19 0.23593363013386412 20-21 0.2288301703310896 22-23 0.2273866865209001 24-25 0.22142281919985413 26-27 0.23061553188579764 28-29 0.24520231565192288 30-31 0.24524030206798048 32-33 0.2450503699876924 34-35 0.25238174828681265 36-37 0.2280324555938796 38-39 0.22639903970340208 40-41 0.22267637092975553 42-43 0.22784252351359152 44-45 0.24664579946211235 46-47 0.251849938462006 48-49 0.2560664306444016 50-51 0.2565982404692082 52-53 0.27870633461474176 54-55 0.25492683816267303 56-57 0.2238539498275417 58-59 0.2077097230030541 60-61 0.20429094555786848 62-63 0.21112850044823972 64-65 0.20432893197392613 66-67 0.2055444972877699 68-69 0.20934313889353168 70-71 0.209609043805935 72-73 0.21192621518544966 74-75 0.21530700621457768 76-77 0.23004573564493336 78-79 0.2224484524334098 80-81 0.21789008250649566 82-83 0.21298983483506298 84-85 0.21234406576208348 86-87 0.21952349839697324 88-89 0.2172443134335162 90-91 0.22848829258657102 92-93 0.21633263944813336 94-95 0.21352164465986964 96-97 0.22377797699542645 98-99 0.23160317870329572 100-101 0.20562047011988516 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1316260.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.10759426357236 #Duplication Level Percentage of deduplicated Percentage of total 1 76.3741509085752 37.505508150431574 2 12.868233428171866 12.638559721592054 3 4.286524756737458 6.315027556638637 4 1.9723359298440797 3.874266903769951 5 1.0686030748067743 2.6238263113208466 6 0.6753048406980527 1.9897557672735804 7 0.4415377581188833 1.517799995442463 8 0.32702798785399034 1.2847646192292983 9 0.2548200587281514 1.1262240048817562 >10 1.5039932945645877 14.205015697182027 >50 0.14467642771980235 4.920527570772428 >100 0.07737527306142106 6.836136562349101 >500 0.0029402560364079245 0.977284873686761 >1k 0.002011754130173843 1.730310986700922 >5k 1.5475031770568025E-4 0.5675243729932139 >10k+ 3.095006354113605E-4 1.887466905735465 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14391 1.0933250269703554 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10446 0.793612204275751 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7468 0.5673651102365794 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3573 0.2714509291477368 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2467 0.18742497682828618 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2433 0.1848419005363682 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1991 0.15126190874143408 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1946 0.14784313129624846 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1788 0.13583942382204123 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1709 0.12983757008493763 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1364 0.10362694300518134 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 3.798641605761779E-5 0.0 16-17 0.0 0.0 0.0 1.1395924817285337E-4 0.0 18-19 0.0 0.0 0.0 1.5194566423047117E-4 0.0 20-21 0.0 0.0 0.0 1.5194566423047117E-4 0.0 22-23 0.0 0.0 0.0 2.6590491240332457E-4 0.0 24-25 0.0 0.0 0.0 3.7986416057617794E-4 0.0 26-27 0.0 0.0 0.0 8.736875693252093E-4 0.0 28-29 0.0 0.0 0.0 0.0022791849634570677 0.0 30-31 0.0 0.0 0.0 0.005584003160469816 0.0 32-33 0.0 0.0 0.0 0.009610563262577303 0.0 34-35 0.0 0.0 0.0 0.013979001109203349 0.0 36-37 0.0 0.0 0.0 0.016638050233236595 0.0 38-39 0.0 0.0 0.0 0.019942868430249342 0.0 40-41 0.0 0.0 0.0 0.023703523619953502 0.0 42-43 0.0 0.0 0.0 0.029021621868019994 0.0 44-45 0.0 0.0 0.0 0.036239040918967375 0.0 46-47 0.0 0.0 0.0 0.04478598453193138 0.0 48-49 0.0 0.0 0.0 0.05432057496239345 0.0 50-51 0.0 0.0 0.0 0.07016091045842007 0.0 52-53 0.0 0.0 0.0 0.10765350310728883 0.0 54-55 0.0 0.0 0.0 0.14799507696047892 0.0 56-57 0.0 0.0 0.0 0.20227766550681475 0.0 58-59 0.0 0.0 0.0 0.27657909531551517 0.0 60-61 0.0 0.0 0.0 0.3848403810797259 0.0 62-63 0.0 0.0 0.0 0.46415601780803184 0.0 64-65 0.0 0.0 0.0 0.5434336681202803 0.0 66-67 0.0 0.0 0.0 0.6774877303876135 0.0 68-69 0.0 0.0 0.0 0.85055384194612 0.0 70-71 0.0 0.0 0.0 1.0169723306945437 0.0 72-73 0.0 0.0 0.0 1.1745019980854847 0.0 74-75 0.0 0.0 0.0 1.4269977056204701 0.0 76-77 0.0 0.0 0.0 1.6282117514776715 0.0 78-79 0.0 0.0 0.0 1.8192454378314316 0.0 80-81 0.0 0.0 0.0 2.019889687447769 0.0 82-83 0.0 0.0 0.0 2.322489477762752 0.0 84-85 0.0 0.0 0.0 2.623645784267546 0.0 86-87 0.0 0.0 0.0 3.0100056219895768 0.0 88-89 0.0 0.0 0.0 3.5241897497454913 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGTATC 55 0.0025733553 34.562965 1 GTATCAA 14705 0.0 30.896305 1 GGTATCA 9825 0.0 29.26419 1 TCAACGC 17870 0.0 25.289286 4 ATCAACG 17865 0.0 25.267838 3 TATCAAC 17905 0.0 25.237928 2 CAACGCA 17990 0.0 25.091316 5 AACGCAG 18250 0.0 24.811012 6 TACCTGG 945 0.0 24.638361 2 GTACATG 10060 0.0 24.328924 1 GAGTACT 9615 0.0 23.714577 12-13 TACATGG 10220 0.0 23.572443 2 AGTACTT 9695 0.0 23.028915 12-13 ACATGGG 10220 0.0 22.735552 3 GTACTTT 10210 0.0 22.309746 14-15 CGCAGAG 20265 0.0 22.225067 8 ACGCAGA 20315 0.0 22.17121 7 ATACCGT 110 0.00287294 21.596916 6 AGAGTAC 15650 0.0 21.551413 10-11 GCAGAGT 20860 0.0 21.089268 9 >>END_MODULE