FastQCFastQC Report
Thu 26 May 2016
SRR937468_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937468_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1316260
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158441.2037135520337927No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125330.9521675049002476No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91360.6940877942047924No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT46570.35380547916065214No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29590.22480361022898213No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24120.18324647106194825No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23160.17595307917888564No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT20670.15703584398219197No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT18360.13948611976357256No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13460.1022594320271071No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG756.9234084E-637.999712
GTATCAA166100.030.484771
GGTATCA113000.028.48481
GTACATG103950.026.0249981
TACATGG106700.024.8850022
TCAACGC206550.024.491464
ATCAACG206100.024.4757983
TATCAAC206900.024.3811592
CAACGCA209300.024.1469735
ACATGGG104550.024.0792053
AGTACTT118550.023.80080812-13
AACGCAG212600.023.7935986
GAGTACT117250.023.65956712-13
CATGGGA68900.023.163834
GTACTTT122200.022.5828514-15
CTGGTCG4000.022.5631879
AGAGTAC177400.022.1709110-11
ATGGGAG25350.021.7355355
ATCACGC1100.002877712721.5907463
TGTAGCG1100.002877712721.5907462
Produced by FastQC (version 0.11.5)