FastQCFastQC Report
Thu 26 May 2016
SRR937467_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937467_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences638511
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36320.5688234031990052No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27600.43225566983184316No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19400.30383188386731No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10510.16460170615698086No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7810.12231582541256142No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14300.056.5128941
GTATCAA27400.042.852981
ATCAACG34850.032.855663
TCAACGC35000.032.448454
TATCAAC36300.031.9358982
CAACGCA36550.031.0723865
AACGCAG37000.030.6896616
GGTTGCG650.00583542229.2376547
AGTATCG700.0083727627.1535135
CGCAGAG42350.026.5883168
ACGCAGA42400.026.5569637
AAGCGCG458.956288E-426.38474794-95
GCAGAGT45000.024.2834959
GTCTTCG1000.001645300523.7686581
TGGTATC6700.023.4010332
GTACATG22950.022.9919031
GAGTACT30600.022.27532812-13
TACATGG22500.022.1718862
AGTACTT33450.021.44245112-13
AGAGTAC43250.021.41702710-11
Produced by FastQC (version 0.11.5)