FastQCFastQC Report
Thu 26 May 2016
SRR937467_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937467_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences638511
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28240.4422789897120018No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23810.37289882241652844No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15380.24087290587006335No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6950.10884698932359818No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15900.048.4143451
GTATCAA26800.038.6525271
ATCAACG33800.030.3545043
TCAACGC34350.029.73024
CAACGCA35100.029.0949385
TATCAAC35400.028.982552
AACGCAG36100.028.6837146
ACGCAGA41650.024.747477
CGCAGAG41500.024.7224628
CACGAGT1000.001653009723.749598
ACGAGTA1000.001653009723.749599
GTATACC1050.002190389222.6275161
TACGCGT2103.4408004E-822.6186565
GCAGAGT45350.022.2046899
CGCGTAT1954.2160718E-721.9226997
GAGTACT27350.021.88261812-13
ACGCGTA2005.379261E-721.374636
GTACTTT28550.021.12923214-15
ATACGCG2056.820592E-720.8532984
TAAGTAC3700.020.5401863
Produced by FastQC (version 0.11.5)