##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937467_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 638511 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.275234099334234 33.0 31.0 34.0 31.0 34.0 2 32.47847257134176 34.0 31.0 34.0 31.0 34.0 3 32.539260874127464 34.0 31.0 34.0 31.0 34.0 4 36.15279298242317 37.0 35.0 37.0 35.0 37.0 5 36.082169297005066 37.0 35.0 37.0 35.0 37.0 6 36.12455697709201 37.0 35.0 37.0 35.0 37.0 7 36.068438914912974 37.0 35.0 37.0 35.0 37.0 8 36.03893433315949 37.0 35.0 37.0 35.0 37.0 9 37.75047728230211 39.0 37.0 39.0 35.0 39.0 10-11 37.66609110884542 39.0 37.0 39.0 35.0 39.0 12-13 37.567634700106964 39.0 37.0 39.0 35.0 39.0 14-15 38.81358269473823 40.0 38.0 41.0 35.0 41.0 16-17 38.76807134097925 40.0 38.0 41.0 35.0 41.0 18-19 38.74877879942554 40.0 38.0 41.0 35.0 41.0 20-21 38.64598182333585 40.0 38.0 41.0 34.0 41.0 22-23 38.59249488262536 40.0 38.0 41.0 34.0 41.0 24-25 38.62496182524655 40.0 38.0 41.0 34.0 41.0 26-27 38.46604991926529 40.0 38.0 41.0 34.0 41.0 28-29 38.32316044672684 40.0 38.0 41.0 34.0 41.0 30-31 38.362613173461384 40.0 38.0 41.0 34.0 41.0 32-33 38.25098236365544 40.0 38.0 41.0 34.0 41.0 34-35 38.131256939974406 40.0 38.0 41.0 34.0 41.0 36-37 37.96490898355705 40.0 37.5 41.0 33.5 41.0 38-39 37.8387850796619 40.0 37.0 41.0 33.0 41.0 40-41 37.64265846633809 40.0 37.0 41.0 33.0 41.0 42-43 37.44497980457659 40.0 37.0 41.0 33.0 41.0 44-45 37.145227725129246 40.0 37.0 41.0 32.0 41.0 46-47 36.79673020511784 40.0 36.0 41.0 31.0 41.0 48-49 36.47384931504704 39.0 36.0 41.0 30.0 41.0 50-51 36.222934295572045 39.0 36.0 41.0 30.0 41.0 52-53 35.986003373473594 39.0 35.0 40.5 29.0 41.0 54-55 35.773541097960724 39.0 35.0 40.0 28.5 41.0 56-57 35.47994866180849 38.5 35.0 40.0 28.0 41.0 58-59 35.23295683238034 38.0 35.0 40.0 28.0 41.0 60-61 34.888660492928075 38.0 34.0 40.0 27.0 41.0 62-63 34.509208925139895 38.0 34.0 40.0 26.0 41.0 64-65 34.103835329383514 37.0 34.0 40.0 26.0 41.0 66-67 33.66434172629759 36.5 33.0 39.0 25.0 40.5 68-69 33.19951418221456 36.0 32.5 39.0 23.5 40.0 70-71 32.74943344750521 35.5 32.0 38.5 22.5 40.0 72-73 32.2657001993701 35.0 31.5 38.0 21.5 40.0 74-75 31.77539306292296 35.0 31.0 37.0 20.0 39.0 76-77 30.468406182508993 33.5 29.5 36.0 19.5 38.5 78-79 31.097307642311563 34.0 31.0 36.0 19.5 38.5 80-81 30.899610969897154 34.0 31.0 36.0 18.0 37.5 82-83 30.48954442445001 34.0 30.5 35.5 16.5 37.0 84-85 30.047902855236636 34.0 30.0 35.0 10.0 36.5 86-87 29.67051468181441 34.0 30.0 35.0 7.0 36.0 88-89 29.20287590973374 34.0 29.0 35.0 2.0 36.0 90-91 28.64248227516832 34.0 29.0 35.0 2.0 35.0 92-93 28.1236572275184 33.0 28.5 35.0 2.0 35.0 94-95 27.63764524025428 33.0 27.0 35.0 2.0 35.0 96-97 26.968588638253685 33.0 26.0 35.0 2.0 35.0 98-99 26.00240481369937 32.5 24.5 34.0 2.0 35.0 100-101 23.026080208485055 29.5 10.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.35512258101147154 1101 2 0.35382416171740516 1101 3 0.26426754633332195 1101 4 0.278823093135955 1101 5 0.34904974117687715 1101 6 0.20824968620843265 1101 7 0.1441026020674272 1101 8 0.20887752617915112 1101 9 0.22503120948869793 1101 10-11 0.3722089123197563 1101 12-13 0.3771386463899731 1101 14-15 0.4583701819817563 1101 16-17 0.42530032839317045 1101 18-19 0.5556121663601132 1101 20-21 0.5179028720672036 1101 22-23 0.41190472985947935 1101 24-25 0.36200044322818314 1101 26-27 0.3311738403467359 1101 28-29 0.29299393831729503 1101 30-31 0.5596171286826319 1101 32-33 0.5618899411278377 1101 34-35 0.7008104842000407 1101 36-37 0.7701307138934723 1101 38-39 0.7296621420216454 1101 40-41 0.6332104427949261 1101 42-43 0.633375006859815 1101 44-45 0.6601485512459888 1101 46-47 0.7307704650076445 1101 48-49 0.4852821341954012 1101 50-51 0.5066824625339947 1101 52-53 0.4955201384201473 1101 54-55 0.5214099272629937 1101 56-57 0.671995232159162 1101 58-59 0.6284656691652089 1101 60-61 0.9119990998995036 1101 62-63 0.8296736613486289 1101 64-65 0.5539960697177619 1101 66-67 0.42197509774228337 1101 68-69 0.6413472681529342 1101 70-71 0.8995658082720155 1101 72-73 1.11044078525061 1101 74-75 0.9543048732121413 1101 76-77 1.0523859807018603 1101 78-79 0.9720271960902629 1101 80-81 1.066266540917379 1101 82-83 0.8094034412711615 1101 84-85 0.8924073940719417 1101 86-87 0.9571705250146962 1101 88-89 1.367268774539454 1101 90-91 1.59645933532272 1101 92-93 1.50962648848056 1101 94-95 1.844759794853175 1101 96-97 2.1056256510041003 1101 98-99 2.6105920543138375 1101 100-101 3.4662066388369723 1102 1 0.29193014735471223 1102 2 0.2685889378185351 1102 3 0.3012924307874272 1102 4 0.1666330775257805 1102 5 0.22218861051054262 1102 6 0.2490565179552391 1102 7 0.18064265287701886 1102 8 0.23862011134642813 1102 9 0.25911158658153255 1102 10-11 0.23986034631320052 1102 12-13 0.1575151270609041 1102 14-15 0.34711616465752826 1102 16-17 0.2945391028414477 1102 18-19 0.3050058182226181 1102 20-21 0.30364802832198734 1102 22-23 0.3465813543888814 1102 24-25 0.23063256345713512 1102 26-27 0.17016438241768128 1102 28-29 0.18205457313104034 1102 30-31 0.21817303453185843 1102 32-33 0.24359053186580581 1102 34-35 0.14391231763030277 1102 36-37 0.1417110144658409 1102 38-39 0.2563261559789751 1102 40-41 0.19520303561424868 1102 42-43 0.2442543800042003 1102 44-45 0.45826553572764084 1102 46-47 0.26407584286568664 1102 48-49 0.13487780007645256 1102 50-51 0.10375754441088958 1102 52-53 0.09533648931237337 1102 54-55 0.18828452553056252 1102 56-57 0.12444847815713445 1102 58-59 0.12456067697029738 1102 60-61 0.3671189309423255 1102 62-63 0.21115509748464945 1102 64-65 0.2658426614964 1102 66-67 0.31729143333041776 1102 68-69 0.33390764279712215 1102 70-71 0.3252258737735332 1102 72-73 0.5065406903652345 1102 74-75 0.2744058802214191 1102 76-77 0.10195777219890445 1102 78-79 0.2063236669665791 1102 80-81 0.3899578879835808 1102 82-83 0.46505585472651845 1102 84-85 0.4302458440734753 1102 86-87 0.6809108451763066 1102 88-89 0.8887318457646991 1102 90-91 1.1359041028535586 1102 92-93 1.081844051456894 1102 94-95 1.2798233068362777 1102 96-97 1.5492545573736614 1102 98-99 2.135040647867754 1102 100-101 2.741612350324221 1103 1 0.22775546965458915 1103 2 0.20224194010960872 1103 3 0.20660389803028778 1103 4 0.0750683409413 1103 5 0.141656363144115 1103 6 0.10207010509278547 1103 7 0.21665392576964848 1103 8 0.13155949203247275 1103 9 0.15790464558463668 1103 10-11 0.27433651306628093 1103 12-13 0.29418157166819725 1103 14-15 0.21417235243865917 1103 16-17 0.23023915468748868 1103 18-19 0.20316383567648444 1103 20-21 0.2696765639176206 1103 22-23 0.32492332390109624 1103 24-25 0.40253476271391264 1103 26-27 0.09277403389066308 1103 28-29 0.3479293084700501 1103 30-31 0.3232937720779461 1103 32-33 0.3952126756270431 1103 34-35 0.4424231198535935 1103 36-37 0.4100214998200613 1103 38-39 0.4918816761337652 1103 40-41 0.48124182456032116 1103 42-43 0.3721135151891204 1103 44-45 0.5143208053415194 1103 46-47 0.4358744948687132 1103 48-49 0.4840123477849474 1103 50-51 0.37034320767811835 1103 52-53 0.19483236624900258 1103 54-55 0.17108988855420648 1103 56-57 0.2346343418570882 1103 58-59 0.021366063165345395 1103 60-61 0.16638425231534626 1103 62-63 0.04303925200322567 1103 64-65 0.1985478081569667 1103 66-67 0.2853199515474927 1103 68-69 0.1970623438424397 1103 70-71 0.03286780592183902 1103 72-73 -0.09998915875990377 1103 74-75 0.09388391516970529 1103 76-77 0.026974354241637855 1103 78-79 0.16782798409053967 1103 80-81 0.4358068740894261 1103 82-83 0.525974948840112 1103 84-85 0.40313456625691657 1103 86-87 0.28611847552275194 1103 88-89 0.404798324549823 1103 90-91 0.7162422896325253 1103 92-93 0.7788488566287555 1103 94-95 0.8137353944937331 1103 96-97 1.0113490214333503 1103 98-99 1.604597031157688 1103 100-101 2.2264582459427977 1104 1 -0.016885714412666175 1104 2 -0.023407574969780853 1104 3 0.0430887933228874 1104 4 0.08102539444716683 1104 5 -0.02062111780090703 1104 6 -0.049771453743403526 1104 7 0.014446460777627124 1104 8 0.05021817375742188 1104 9 -0.06248417718510524 1104 10-11 -0.08647987547794145 1104 12-13 0.06471156584408533 1104 14-15 0.03485934441151528 1104 16-17 -0.08261377382786605 1104 18-19 -0.0172423961231587 1104 20-21 0.16568451509637327 1104 22-23 -0.07112577589379754 1104 24-25 -0.009986444649797477 1104 26-27 0.028647080914034007 1104 28-29 -0.06882704107794524 1104 30-31 -0.08830633933984444 1104 32-33 0.03189729997183122 1104 34-35 0.00408115586179747 1104 36-37 0.011079335782682165 1104 38-39 0.08103490091915688 1104 40-41 -0.04979531048310548 1104 42-43 -0.1349371419226273 1104 44-45 0.10098612148949826 1104 46-47 0.08923380458484331 1104 48-49 0.34133110395724486 1104 50-51 0.0762081515893911 1104 52-53 0.09478425402089385 1104 54-55 0.1110192395455698 1104 56-57 0.051029216638681874 1104 58-59 0.10919620167791777 1104 60-61 0.392237927836355 1104 62-63 0.16936392091513142 1104 64-65 0.28130875368885455 1104 66-67 0.4033231041645422 1104 68-69 0.2498781752977024 1104 70-71 0.3116022011004844 1104 72-73 0.5757866509182534 1104 74-75 0.540303669037673 1104 76-77 0.5451462161848646 1104 78-79 0.4127951918087547 1104 80-81 0.5850924708076626 1104 82-83 0.3956129676292015 1104 84-85 0.3703549455838484 1104 86-87 0.2877438458602981 1104 88-89 0.5310438615076123 1104 90-91 0.4408599240302351 1104 92-93 0.6487226886411115 1104 94-95 0.4937415984389375 1104 96-97 0.8854390039610145 1104 98-99 0.9128318188308278 1104 100-101 1.5489193763269355 1105 1 0.026246790327171254 1105 2 0.07968385119411892 1105 3 0.22110912195149268 1105 4 0.07637772223313277 1105 5 -0.007402242721909147 1105 6 0.010858944402798443 1105 7 -0.1312639879268218 1105 8 0.07078379414924996 1105 9 0.1600642744685672 1105 10-11 0.14290183928927291 1105 12-13 0.1468489684141261 1105 14-15 0.17865410952632743 1105 16-17 0.14802531401708308 1105 18-19 0.2507623963773611 1105 20-21 0.18921253279176398 1105 22-23 -0.009455247138788536 1105 24-25 0.0073073724978769405 1105 26-27 0.006922834021253266 1105 28-29 -0.02389076553549785 1105 30-31 0.1593303259224257 1105 32-33 0.04352306361202807 1105 34-35 0.25872882807583153 1105 36-37 0.15221452642941102 1105 38-39 0.1502760005610284 1105 40-41 0.06155791260515997 1105 42-43 -0.253044630863009 1105 44-45 -0.0069438869368738665 1105 46-47 0.04484392676727822 1105 48-49 0.12443753960080528 1105 50-51 0.2626219392447453 1105 52-53 0.14696852829938223 1105 54-55 0.34371225618765777 1105 56-57 0.35587964849038656 1105 58-59 0.29601814185066644 1105 60-61 0.40228374624798136 1105 62-63 0.5252860822802035 1105 64-65 0.1691326424607098 1105 66-67 0.15559427543060877 1105 68-69 0.11040851024609566 1105 70-71 0.11242377261934422 1105 72-73 0.08961775477332168 1105 74-75 0.09218512048068916 1105 76-77 0.18493845338731063 1105 78-79 0.4252438435888273 1105 80-81 0.4308108192382498 1105 82-83 0.30374573569324426 1105 84-85 0.24266903869528988 1105 86-87 0.4051140270273521 1105 88-89 0.8215233748844781 1105 90-91 0.8697953042703901 1105 92-93 0.6732974200504245 1105 94-95 0.9869478339561013 1105 96-97 1.2169575946498732 1105 98-99 1.0596087305645554 1105 100-101 1.622557990188323 1106 1 -0.19035593028671371 1106 2 0.07231552193007929 1106 3 0.12647623042725087 1106 4 0.1255798498927092 1106 5 0.13734836767223157 1106 6 0.020123855459651452 1106 7 0.09408044876659005 1106 8 -0.07723678485319141 1106 9 0.024534975549840965 1106 10-11 -0.191485150595625 1106 12-13 -0.3018888237030737 1106 14-15 -0.07364576839484727 1106 16-17 -0.09882461622358818 1106 18-19 -0.0983067525588055 1106 20-21 -0.050585165150344835 1106 22-23 0.17361939534463744 1106 24-25 0.034568010795752 1106 26-27 0.029681815535035128 1106 28-29 0.07627927283213154 1106 30-31 0.19943104095555952 1106 32-33 0.2345328299182654 1106 34-35 -0.2371946110591594 1106 36-37 0.24991028855009745 1106 38-39 0.3328710476485881 1106 40-41 0.04066281319811793 1106 42-43 0.168290824316621 1106 44-45 0.24646778031111438 1106 46-47 0.2886636860975855 1106 48-49 0.31859737566637847 1106 50-51 0.48906726536962 1106 52-53 0.4449607675738889 1106 54-55 0.43842144697943297 1106 56-57 0.46727657907985076 1106 58-59 0.4985708973442087 1106 60-61 0.5852057901963619 1106 62-63 0.6407159741532382 1106 64-65 0.6533059943965327 1106 66-67 0.5573971181233119 1106 68-69 0.7525867453350372 1106 70-71 0.9799900439831362 1106 72-73 0.6724024600401535 1106 74-75 0.6391309698005365 1106 76-77 0.4960977662579076 1106 78-79 0.824081914743342 1106 80-81 0.6671205506648299 1106 82-83 0.5951544381697005 1106 84-85 0.6480797467524937 1106 86-87 0.7751545746380941 1106 88-89 1.045570724029929 1106 90-91 1.027767487737428 1106 92-93 0.8301360143999155 1106 94-95 0.325018988473019 1106 96-97 1.1145312779425147 1106 98-99 1.0325769900692663 1106 100-101 1.23321372789324 1107 1 0.047687666965536835 1107 2 -0.0032646800458948633 1107 3 0.017185472606840335 1107 4 -0.06265053408058208 1107 5 -0.06195237947465415 1107 6 0.06700526983880906 1107 7 -4.897365148934796E-4 1107 8 0.08349558330138507 1107 9 0.015010905378240125 1107 10-11 -0.0918022775011238 1107 12-13 -0.06691044443707739 1107 14-15 -0.03846418159718468 1107 16-17 -0.05612366655336842 1107 18-19 -0.04884932351038884 1107 20-21 0.031855856771443314 1107 22-23 0.08859380624806334 1107 24-25 0.13911725954366716 1107 26-27 0.23506681512451166 1107 28-29 0.06969384356477804 1107 30-31 0.08818004886492048 1107 32-33 0.18491119276345103 1107 34-35 -0.06290652162046939 1107 36-37 0.14581090159369126 1107 38-39 -0.0013796844458937585 1107 40-41 0.07661070449234586 1107 42-43 -0.27617604669791973 1107 44-45 0.014245018330768744 1107 46-47 0.2878186985500335 1107 48-49 0.30698136263920617 1107 50-51 0.31309946384248377 1107 52-53 0.3301228546589243 1107 54-55 0.15890846085501664 1107 56-57 0.12891893382409592 1107 58-59 0.22835106374175496 1107 60-61 0.21758318147690403 1107 62-63 0.22177491117942338 1107 64-65 0.4493270267181586 1107 66-67 0.5023856919759098 1107 68-69 0.5746108155066381 1107 70-71 0.45563763970277904 1107 72-73 0.5602192448454204 1107 74-75 0.5544970854577791 1107 76-77 0.49060192619660015 1107 78-79 0.3134340453435236 1107 80-81 0.6311025288251528 1107 82-83 0.4297147709647966 1107 84-85 0.49138587171486137 1107 86-87 0.875027313355627 1107 88-89 0.6795080923213419 1107 90-91 0.8146202341180064 1107 92-93 0.9238646331360627 1107 94-95 0.6231011746302784 1107 96-97 0.9264035240357273 1107 98-99 1.0473009836104126 1107 100-101 1.4224327815071867 1108 1 0.1647584548274068 1108 2 0.28176020065022556 1108 3 0.05951218003454528 1108 4 0.091013933493997 1108 5 0.07012734611300431 1108 6 0.14041271740683214 1108 7 0.09010069018770395 1108 8 0.12981608531706712 1108 9 0.35403790106773414 1108 10-11 0.010444768754965139 1108 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50-51 0.3113516894016115 2316 52-53 0.1807675842574028 2316 54-55 0.21261056489794328 2316 56-57 0.20167424092663566 2316 58-59 0.067674422154127 2316 60-61 0.3607216562360023 2316 62-63 0.6368456054106559 2316 64-65 0.467657398606967 2316 66-67 0.3330887851562352 2316 68-69 0.7642253204679861 2316 70-71 0.5923735758250928 2316 72-73 0.33298842672586204 2316 74-75 0.422794113122297 2316 76-77 0.5675196666666444 2316 78-79 0.6069594473764042 2316 80-81 0.4737312173900996 2316 82-83 0.6351711392640631 2316 84-85 0.7410647377426898 2316 86-87 0.860758670801232 2316 88-89 1.117201388118179 2316 90-91 0.9081541400470137 2316 92-93 0.9321335531860129 2316 94-95 1.4341199861436564 2316 96-97 1.5179725823858838 2316 98-99 1.638782765131893 2316 100-101 1.9828126818773306 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 5.0 5 12.0 6 29.0 7 63.0 8 147.0 9 300.0 10 506.0 11 868.0 12 1332.0 13 1909.0 14 2454.0 15 3309.0 16 4489.0 17 6526.0 18 5868.0 19 3739.0 20 3184.0 21 3240.0 22 3504.0 23 3774.0 24 4306.0 25 5148.0 26 6203.0 27 7517.0 28 9351.0 29 11605.0 30 14365.0 31 18376.0 32 23532.0 33 31266.0 34 42976.0 35 62135.0 36 95614.0 37 143111.0 38 109016.0 39 8731.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.05733267523758 20.553583879787844 15.585744392475773 21.803339052498806 2 15.652666907852803 21.3297813193508 40.21481227418165 22.802739498614745 3 18.99982929033329 25.77794274491747 30.281075815451885 24.94115214929735 4 13.017316851236703 18.04166255553937 37.62143486956372 31.319585723660204 5 13.13678044807793 38.244336379511516 34.56167140429597 14.05721176811458 6 30.630482481899296 38.04445029138105 17.41865057923826 13.906416647481407 7 27.56318998419761 33.66238013127417 21.17896167802904 17.595468206499184 8 25.995793337937794 36.08504786918314 19.66418746114006 18.254971331738997 9 26.16102150158729 17.438697218998573 20.6128007191732 35.78748056024094 10-11 24.17726554436807 27.91244003627189 27.471570575918037 20.43872384344201 12-13 25.309076556698816 25.355515372971045 28.073399955675598 21.26200811465454 14-15 22.390217239796964 26.880429624548363 26.472762411297534 24.25659072435714 16-17 21.28177901398723 30.088909979624468 27.135397823999902 21.4939131823884 18-19 21.21631420601994 28.282989643091504 29.978888382502415 20.521807768386136 20-21 21.885874927565737 27.390408920768667 30.473289376830433 20.250426774835162 22-23 21.663880220670862 27.25754983300901 30.377090323919454 20.701479622400676 24-25 21.48312361347229 27.659607790318248 30.275383098633462 20.581885497576 26-27 21.283135355838738 27.77947011621797 30.27493408363528 20.662460444308014 28-29 21.12108694375379 27.968441042307013 30.222674680397034 20.68779733354216 30-31 21.372430637288872 27.848763409158362 30.18498550139035 20.59382045216242 32-33 21.284631407495574 27.797372006703107 30.095613224538383 20.822383361262943 34-35 21.573551591123724 27.853944567908773 29.977322238771144 20.595181602196362 36-37 21.70471612861799 27.621293916627902 29.96111265115245 20.71287730360166 38-39 21.40278037714337 27.974716155803232 29.88784052885583 20.73466293819757 40-41 21.630089379822746 27.964201086590524 29.799251696525197 20.60645783706154 42-43 21.373270431385816 28.074223045043183 29.600879624843763 20.951626898727238 44-45 21.527115429491424 28.229897370601286 29.49346213299379 20.749525066913492 46-47 21.874877645020995 28.456596675703317 28.7619947033019 20.906530975973787 48-49 21.773078302488134 28.63028201550169 28.598176069010556 20.99846361299962 50-51 21.79289002068876 28.82667643940355 28.520260418379635 20.860173121528057 52-53 21.939011230816696 28.703577542125352 28.39904089357897 20.958370333478985 54-55 21.700487540543545 28.896917985751223 28.37390428669201 21.028690187013225 56-57 21.71520929161753 29.20043664087228 28.21854282854955 20.865811238960642 58-59 21.682320273260757 28.709372273931066 28.309927315269434 21.298380137538743 60-61 21.54042765120726 28.94061339585379 28.654478936149886 20.86448001678906 62-63 21.47026441204615 28.82941719093328 28.84758445821607 20.852733938804498 64-65 21.57073253240743 28.865908339871982 28.609452303875738 20.95390682384485 66-67 21.46861996112831 28.868257555468897 28.458397740994286 21.20472474240851 68-69 21.50417142382825 28.85870407870812 28.43772464374145 21.199399853722177 70-71 21.605735844801423 28.861523137424417 28.375313816050152 21.157427201724012 72-73 21.592065903431426 28.79375420901787 28.157272399805798 21.456907487744907 74-75 21.592580237458712 29.254468599601257 27.88111716164639 21.271834001293634 76-77 21.67041758090307 28.85369241876804 28.14438592287369 21.331504077455204 78-79 21.747080316548974 28.907176227191073 27.98111543888829 21.36462801737167 80-81 21.510827534686168 29.207719209222706 27.95707513261322 21.324378123477906 82-83 21.663482169425695 29.26461605926297 27.80716042035364 21.264741350957696 84-85 21.508293925149115 29.376618515464532 27.698726487233156 21.416361072153194 86-87 21.29689230099403 29.15634969483689 27.890044180914657 21.656713823254414 88-89 21.40307684597446 29.070211789616778 27.80406289006767 21.72264847434108 90-91 21.580975353794827 29.230461989082677 27.716290616899137 21.47227204022336 92-93 21.23703025035043 29.213945074823222 27.786469957165565 21.762554717660787 94-95 21.170426194693594 29.222127731550433 27.64314162167919 21.964304452076785 96-97 21.445832569838263 29.355954713387867 27.399371349906264 21.798841366867602 98-99 21.118344135885852 29.646526699414967 27.17748370611732 22.057645458581863 100-101 21.142071272941436 29.45878803732345 27.397445878020548 22.001694811714565 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 266.0 1 175.5 2 94.5 3 119.5 4 120.0 5 141.0 6 222.0 7 380.0 8 613.5 9 776.0 10 793.0 11 806.5 12 882.0 13 951.5 14 1011.0 15 1067.0 16 1107.0 17 1133.0 18 1200.0 19 1324.0 20 1459.5 21 1675.0 22 1938.0 23 2272.0 24 2764.5 25 3481.5 26 4484.0 27 5669.5 28 6744.0 29 7860.0 30 9355.5 31 10751.0 32 12306.0 33 13896.0 34 15685.0 35 17038.0 36 18636.5 37 20680.5 38 21743.0 39 22159.0 40 22262.0 41 22755.5 42 22957.0 43 23386.5 44 24327.5 45 24882.0 46 24502.5 47 23646.5 48 23455.0 49 22807.5 50 21759.5 51 20195.0 52 18679.0 53 18269.0 54 17777.0 55 16152.5 56 13690.5 57 12003.0 58 10862.5 59 9362.0 60 7592.0 61 6067.0 62 4876.5 63 3620.5 64 2465.5 65 1720.5 66 1263.0 67 959.0 68 721.5 69 508.5 70 368.0 71 267.0 72 193.0 73 155.5 74 109.0 75 79.5 76 62.0 77 32.0 78 20.5 79 16.0 80 10.0 81 7.5 82 6.5 83 4.0 84 2.0 85 1.5 86 1.0 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.047923998177008695 2 0.0 3 0.0 4 0.0 5 9.396862387648765E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005716424619152998 14-15 0.0 16-17 0.0 18-19 0.0 20-21 1.566143731274794E-4 22-23 5.481503059461779E-4 24-25 7.83071865637397E-5 26-27 0.0019576796640934924 28-29 0.0036804377684957657 30-31 2.3492155969121912E-4 32-33 1.566143731274794E-4 34-35 0.0 36-37 0.0 38-39 7.83071865637397E-5 40-41 0.0 42-43 0.008927019268266325 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 1.566143731274794E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.566143731274794E-4 84-85 2.3492155969121912E-4 86-87 0.0 88-89 0.0 90-91 0.011902692357688434 92-93 9.396862387648765E-4 94-95 0.0 96-97 0.0 98-99 7.047646790736573E-4 100-101 0.014408522327728103 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 638511.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.8327090019116 #Duplication Level Percentage of deduplicated Percentage of total 1 84.82168124557363 57.53684420983899 2 9.12808086676625 12.383649063625443 3 2.4595268547394107 5.00509108259776 4 1.0780125339713478 2.924980420691671 5 0.5847373318493814 1.983215863694665 6 0.40866767968758544 1.6632621476840643 7 0.2737385085307023 1.29978972142463 8 0.19199775805117417 1.041898244072378 9 0.15119041562545732 0.9230089920299743 >10 0.8398518958910374 10.092118521577351 >50 0.04468663413121318 2.1052008237287203 >100 0.01690213101759307 1.8727496849490253 >500 2.3153604131972757E-4 0.10915473255435484 >1k 6.946081239591829E-4 1.0590364915309565 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2824 0.4422789897120018 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2381 0.37289882241652844 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1538 0.24087290587006335 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 695 0.10884698932359818 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 1.566143731274794E-4 0.0 4 0.0 0.0 0.0 1.566143731274794E-4 0.0 5 0.0 0.0 0.0 1.566143731274794E-4 0.0 6 0.0 0.0 0.0 1.566143731274794E-4 0.0 7 0.0 0.0 0.0 1.566143731274794E-4 0.0 8 0.0 0.0 0.0 1.566143731274794E-4 0.0 9 0.0 0.0 0.0 1.566143731274794E-4 0.0 10-11 0.0 0.0 0.0 1.566143731274794E-4 0.0 12-13 0.0 0.0 0.0 1.566143731274794E-4 0.0 14-15 0.0 0.0 0.0 1.566143731274794E-4 0.0 16-17 0.0 0.0 0.0 3.915359328186985E-4 0.0 18-19 7.83071865637397E-5 0.0 0.0 4.698431193824382E-4 0.0 20-21 1.566143731274794E-4 0.0 0.0 4.698431193824382E-4 0.0 22-23 1.566143731274794E-4 0.0 0.0 8.613790522011366E-4 0.0 24-25 1.566143731274794E-4 0.0 0.0 0.0010963006118923557 0.0 26-27 1.566143731274794E-4 0.0 0.0 0.001331222171583575 0.0 28-29 1.566143731274794E-4 0.0 0.0 0.0024275227834759305 0.0 30-31 1.566143731274794E-4 0.0 0.0 0.003680437768495766 0.0 32-33 1.566143731274794E-4 0.0 0.0 0.006499496484790396 0.0 34-35 1.566143731274794E-4 0.0 0.0 0.008143947402628929 0.0 36-37 1.566143731274794E-4 0.0 0.0 0.01080639174579608 0.0 38-39 1.566143731274794E-4 0.0 0.0 0.014173600768036886 0.0 40-41 1.566143731274794E-4 0.0 0.0 0.01824557446935135 0.0 42-43 1.566143731274794E-4 0.0 0.0 0.027799051230127596 0.0 44-45 1.566143731274794E-4 0.0 0.0 0.04369541010256675 0.0 46-47 1.566143731274794E-4 0.0 0.0 0.06241082769130055 0.0 48-49 1.566143731274794E-4 0.0 0.0 0.10172103534629787 0.0 50-51 1.566143731274794E-4 0.0 0.0 0.1450249095160459 0.0 52-53 1.566143731274794E-4 0.0 0.0 0.18503988185011694 0.0 54-55 1.566143731274794E-4 0.0 0.0 0.2536369772799529 0.0 56-57 1.566143731274794E-4 0.0 0.0 0.3225473014560438 0.0 58-59 1.566143731274794E-4 0.0 0.0 0.4144016312953105 0.0 60-61 1.566143731274794E-4 0.0 0.0 0.5112676210746565 0.0 62-63 1.566143731274794E-4 0.0 0.0 0.6523771712625155 0.0 64-65 1.566143731274794E-4 0.0 0.0 0.754802971287887 0.0 66-67 1.566143731274794E-4 0.0 0.0 0.9032733970127375 0.0 68-69 1.566143731274794E-4 0.0 0.0 1.1120403563916674 0.0 70-71 1.566143731274794E-4 0.0 0.0 1.2624684617806115 0.0 72-73 1.566143731274794E-4 0.0 0.0 1.4879148518976182 0.0 74-75 1.566143731274794E-4 0.0 0.0 1.759875710833486 0.0 76-77 1.566143731274794E-4 0.0 0.0 1.9864184015623851 0.0 78-79 1.566143731274794E-4 0.0 0.0 2.2131960138509754 0.0 80-81 1.566143731274794E-4 0.0 0.0 2.5376226877845487 0.0 82-83 1.566143731274794E-4 0.0 0.0 2.9260263331406975 0.0 84-85 1.566143731274794E-4 0.0 0.0 3.3546798723906086 0.0 86-87 1.566143731274794E-4 0.0 0.0 3.8487199124212426 0.0 88-89 1.566143731274794E-4 0.0 0.0 4.5409554416447016 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1590 0.0 48.414345 1 GTATCAA 2680 0.0 38.652527 1 ATCAACG 3380 0.0 30.354504 3 TCAACGC 3435 0.0 29.7302 4 CAACGCA 3510 0.0 29.094938 5 TATCAAC 3540 0.0 28.98255 2 AACGCAG 3610 0.0 28.683714 6 ACGCAGA 4165 0.0 24.74747 7 CGCAGAG 4150 0.0 24.722462 8 CACGAGT 100 0.0016530097 23.74959 8 ACGAGTA 100 0.0016530097 23.74959 9 GTATACC 105 0.0021903892 22.627516 1 TACGCGT 210 3.4408004E-8 22.618656 5 GCAGAGT 4535 0.0 22.204689 9 CGCGTAT 195 4.2160718E-7 21.922699 7 GAGTACT 2735 0.0 21.882618 12-13 ACGCGTA 200 5.379261E-7 21.37463 6 GTACTTT 2855 0.0 21.129232 14-15 ATACGCG 205 6.820592E-7 20.853298 4 TAAGTAC 370 0.0 20.540186 3 >>END_MODULE