FastQCFastQC Report
Thu 26 May 2016
SRR937466_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937466_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences629075
Sequences flagged as poor quality0
Sequence length101
%GC42

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37630.5981798672654294No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30230.4805468346381592No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19630.31204546357747487No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8940.14211342049835074No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7740.12303779358582044No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14700.057.282751
GTATCAA27150.042.75511
ATCAACG34950.033.061243
TATCAAC35900.032.3213962
TCAACGC36000.032.0995184
CAACGCA36650.031.530225
AACGCAG37800.030.662536
TGGTATC6300.029.438712
ATACGAT1004.897452E-528.5442871
CGCAGAG42350.027.2538748
ACGCAGA42500.027.1598477
GTACATG20850.026.0115811
TACATGG20800.024.9205652
GCAGAGT45150.024.7221079
ACATGGG20200.023.0693193
GTGGTAT8150.022.7653831
CTAGACT2103.398054E-822.645164
CTGTGCG1050.002195654222.6180979
CATGGGT5550.022.277944
GAGTACT31850.022.21687912-13
Produced by FastQC (version 0.11.5)