FastQCFastQC Report
Thu 26 May 2016
SRR937466_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937466_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences629075
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28910.4595636450343759No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24060.38246631959623256No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15360.24416802448038788No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6970.11079759965028017No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17050.050.1705971
GTACGAC554.960713E-543.1818543
GTATCAA28150.037.6467631
ATGGGCG600.00394958831.6666935
ATCAACG35000.030.1285973
TCAACGC35250.029.9149194
TATCAAC35750.029.4965292
CAACGCA36000.029.2916935
AACGCAG36850.028.7426516
TCCGTGC908.934684E-426.3931068
ACGCAGA42250.024.962577
GGACCGA1151.281536E-424.780666
CGCAGAG42450.024.7330448
GTGCATA2402.2737368E-1023.7613531
GTATAGC2202.1518645E-923.7613531
GAGTACT27000.022.96200412-13
GCAGAGT45600.022.7119649
CAGCGTA1050.002193085222.6226639
ATATCGT550.002878829121.58921292-93
GTACTTT29000.020.9638714-15
Produced by FastQC (version 0.11.5)