##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937466_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 629075 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.24857290466161 33.0 31.0 34.0 31.0 34.0 2 32.470811906370464 34.0 31.0 34.0 31.0 34.0 3 32.54538330087827 34.0 31.0 34.0 31.0 34.0 4 36.146634344076624 37.0 35.0 37.0 35.0 37.0 5 36.00906569169018 37.0 35.0 37.0 35.0 37.0 6 36.09558319755196 37.0 35.0 37.0 35.0 37.0 7 36.06643722926519 37.0 35.0 37.0 35.0 37.0 8 36.03823868378174 37.0 35.0 37.0 35.0 37.0 9 37.75366371259389 39.0 37.0 39.0 35.0 39.0 10-11 37.676117315105515 39.0 37.0 39.0 35.0 39.0 12-13 37.6004379446012 39.0 37.0 39.0 35.0 39.0 14-15 38.8458061439415 40.0 38.0 41.0 35.0 41.0 16-17 38.814882168262926 40.0 38.0 41.0 35.0 41.0 18-19 38.82567102491754 40.0 38.0 41.0 35.0 41.0 20-21 38.734533243253985 40.0 38.0 41.0 34.0 41.0 22-23 38.60427850415292 40.0 38.0 41.0 34.0 41.0 24-25 38.467129515558554 40.0 38.0 41.0 34.0 41.0 26-27 38.254355998887256 40.0 38.0 41.0 34.0 41.0 28-29 38.247922743711 40.0 38.0 41.0 34.0 41.0 30-31 38.37046695545047 40.0 38.0 41.0 34.0 41.0 32-33 38.3214068274848 40.0 38.0 41.0 34.0 41.0 34-35 38.2116456702301 40.0 38.0 41.0 34.0 41.0 36-37 38.064385804554306 40.0 38.0 41.0 34.0 41.0 38-39 37.91478837976394 40.0 37.0 41.0 33.0 41.0 40-41 37.756106187656485 40.0 37.0 41.0 33.0 41.0 42-43 37.55900329849382 40.0 37.0 41.0 33.0 41.0 44-45 37.28827882208004 40.0 37.0 41.0 32.5 41.0 46-47 36.95964233199539 40.0 37.0 41.0 31.5 41.0 48-49 36.64722727814649 40.0 36.0 41.0 31.0 41.0 50-51 36.43673806779796 39.5 36.0 41.0 30.0 41.0 52-53 36.212494535627705 39.0 36.0 41.0 30.0 41.0 54-55 36.018991376227 39.0 35.0 41.0 29.5 41.0 56-57 35.789188093629534 39.0 35.0 40.0 29.0 41.0 58-59 35.55029686444382 39.0 35.0 40.0 28.0 41.0 60-61 35.2383674442634 38.0 35.0 40.0 28.0 41.0 62-63 34.88186543734849 38.0 34.0 40.0 27.0 41.0 64-65 34.54583873147081 37.5 34.0 40.0 26.0 41.0 66-67 34.164470850057626 37.0 34.0 39.5 26.0 41.0 68-69 33.72971346818742 36.0 33.0 39.0 26.0 40.5 70-71 33.35111393712991 36.0 33.0 39.0 25.0 40.0 72-73 32.919933235305805 35.0 33.0 38.5 24.0 40.0 74-75 32.478036800063585 35.0 32.0 37.5 23.5 39.5 76-77 31.16515916226205 34.0 30.5 36.0 21.5 39.0 78-79 31.754460914835274 35.0 32.0 36.0 22.0 39.0 80-81 31.571740253546874 35.0 32.0 36.0 21.5 38.5 82-83 31.246507173230537 35.0 31.5 36.0 20.5 37.0 84-85 30.90472916583873 34.0 31.0 35.0 19.5 37.0 86-87 30.57096610102134 34.0 31.0 35.0 18.0 36.5 88-89 30.22714938600326 34.0 31.0 35.0 16.5 36.0 90-91 29.798539919723403 34.0 30.5 35.0 8.5 36.0 92-93 29.401399674124708 34.0 30.0 35.0 4.5 35.0 94-95 29.01873067599253 34.0 29.5 35.0 2.0 35.0 96-97 28.623377975599094 34.0 29.0 35.0 2.0 35.0 98-99 28.016035448873346 34.0 29.0 35.0 2.0 35.0 100-101 26.446992012081232 32.0 26.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.058628515859538766 1101 2 0.14333297212773743 1101 3 0.11620749785154061 1101 4 -0.00836622982815527 1101 5 -0.04197357713151462 1101 6 0.022094237819239027 1101 7 0.08221116325661626 1101 8 -0.054358001765010044 1101 9 0.1445214988188397 1101 10-11 0.19250389718754946 1101 12-13 0.20209759069776823 1101 14-15 0.10318310788622398 1101 16-17 0.063799246180686 1101 18-19 0.17425169058802226 1101 20-21 0.06879648931939641 1101 22-23 0.14595630271344362 1101 24-25 0.26193432880864265 1101 26-27 0.2137044434409603 1101 28-29 0.11881597438393499 1101 30-31 0.22143106678054636 1101 32-33 0.1401830344840178 1101 34-35 0.015506618514855575 1101 36-37 0.030028265938490506 1101 38-39 0.10989449039451671 1101 40-41 0.03580348408257805 1101 42-43 0.10572356531898919 1101 44-45 0.014740223350656834 1101 46-47 0.018212569585223548 1101 48-49 -0.047715467637495124 1101 50-51 -0.019976548080762768 1101 52-53 -0.06722845783046694 1101 54-55 -0.2681143653258218 1101 56-57 -0.08668262081595657 1101 58-59 -0.22979461190728756 1101 60-61 0.09219644251516712 1101 62-63 0.19938197908247446 1101 64-65 -0.05029276518200021 1101 66-67 0.02202063724433856 1101 68-69 -0.08546325298466684 1101 70-71 -0.004394668280724545 1101 72-73 0.03974284104311465 1101 74-75 0.21301827506246696 1101 76-77 0.2370253582954298 1101 78-79 0.1312984046638519 1101 80-81 0.22510316808794428 1101 82-83 0.10905848826574172 1101 84-85 0.08697746844224952 1101 86-87 0.06016371826468969 1101 88-89 0.11213789952752506 1101 90-91 0.30663552056329024 1101 92-93 0.3326269017287977 1101 94-95 0.05656180296131552 1101 96-97 -0.05256994808767601 1101 98-99 -0.35033712923809546 1101 100-101 -0.06708961028632388 1102 1 0.22175887505122205 1102 2 0.2893533345260124 1102 3 0.21228064652346745 1102 4 0.1736297624632357 1102 5 0.010887268450261445 1102 6 0.06792508098275363 1102 7 0.23228717336004223 1102 8 0.26731015101370303 1102 9 0.329515282715775 1102 10-11 0.3530162489625255 1102 12-13 0.15843519274146445 1102 14-15 0.32721156879614455 1102 16-17 0.30096142201917075 1102 18-19 0.3744762586301178 1102 20-21 0.2683946531453998 1102 22-23 0.23190471385193234 1102 24-25 0.2985097300855202 1102 26-27 0.321719754123599 1102 28-29 0.5015665280805166 1102 30-31 0.38770463284222245 1102 32-33 0.2216230397679695 1102 34-35 0.17238784993178768 1102 36-37 0.2664649634246672 1102 38-39 0.35733001497075634 1102 40-41 0.2918403481837544 1102 42-43 0.36053265585037053 1102 44-45 0.35246637604627296 1102 46-47 -0.01635524879164052 1102 48-49 -0.0162136209169077 1102 50-51 0.10314011118197186 1102 52-53 0.06479475321555128 1102 54-55 0.004907853913529436 1102 56-57 -0.06751337342783614 1102 58-59 -0.0058412555844995495 1102 60-61 -0.0033829334456143556 1102 62-63 0.07274499204415008 1102 64-65 0.12048920673421293 1102 66-67 0.07900477792534844 1102 68-69 0.06520560396613462 1102 70-71 -0.010382261642114088 1102 72-73 -0.013661314317921835 1102 74-75 0.05884453737882467 1102 76-77 -0.18834784758382028 1102 78-79 -0.08364027057645629 1102 80-81 -0.10643511234725977 1102 82-83 -0.19196708884110336 1102 84-85 -0.17476840959429296 1102 86-87 -0.004108763308053653 1102 88-89 -0.025285917255637003 1102 90-91 -0.08114810578446452 1102 92-93 -0.1878500177260598 1102 94-95 -0.1357773160885074 1102 96-97 -0.19270445454400686 1102 98-99 -0.16409852675868564 1102 100-101 -0.05424566057337188 1103 1 0.1262225565746249 1103 2 -0.015268281856400279 1103 3 0.01087088762387367 1103 4 0.09948957386903601 1103 5 0.010573301561876747 1103 6 0.05967274674596723 1103 7 0.06435770174015687 1103 8 0.02151570180557627 1103 9 0.012581169380865731 1103 10-11 0.04959579378189005 1103 12-13 0.018410717595394033 1103 14-15 0.11735384098879109 1103 16-17 0.05470791516363249 1103 18-19 -0.08423709554713099 1103 20-21 0.07710259615090109 1103 22-23 0.08617888543721364 1103 24-25 0.04159502561279993 1103 26-27 0.16350244384463508 1103 28-29 0.14209584771188588 1103 30-31 0.05248967850418751 1103 32-33 0.11950114688062996 1103 34-35 0.22399794111300508 1103 36-37 0.16313536835870224 1103 38-39 0.044553584369928956 1103 40-41 0.2690301780351021 1103 42-43 0.22931947114388862 1103 44-45 0.0018884510106289554 1103 46-47 0.1312745985181749 1103 48-49 -0.12811486881636114 1103 50-51 -0.12594511536773467 1103 52-53 7.805914060554642E-4 1103 54-55 -0.10159233101772713 1103 56-57 0.08102507502866985 1103 58-59 -0.11417291403133589 1103 60-61 -0.2929811152919015 1103 62-63 -0.2130933173868712 1103 64-65 -0.08105196633646727 1103 66-67 -0.04110599054428832 1103 68-69 -0.07241773804490492 1103 70-71 -0.15216090785019531 1103 72-73 -0.1778921608511368 1103 74-75 -0.0702226122894345 1103 76-77 -0.4699095474290971 1103 78-79 -0.38490041949665965 1103 80-81 -0.16179972423542566 1103 82-83 -0.20805551333384997 1103 84-85 -0.3564993423494798 1103 86-87 -0.0626296938393125 1103 88-89 -0.2799642022828621 1103 90-91 -0.1352330647487534 1103 92-93 -0.2874821769738709 1103 94-95 -0.31293015968899596 1103 96-97 0.09902311258128194 1103 98-99 -0.0778610988134929 1103 100-101 0.12606528557298446 1104 1 0.016961849192867362 1104 2 0.11285116288486563 1104 3 -0.003998991823976894 1104 4 0.11663377017184473 1104 5 0.018129667706247687 1104 6 0.02406080615748607 1104 7 0.07827802658011507 1104 8 0.06584848791050746 1104 9 0.03795807698993059 1104 10-11 0.15870350387388044 1104 12-13 0.1986560961058288 1104 14-15 0.31292991221266675 1104 16-17 0.2097554900351568 1104 18-19 0.2346007564680619 1104 20-21 0.09559098292805146 1104 22-23 0.16236485728571637 1104 24-25 0.05501446646842112 1104 26-27 -0.042686903658349706 1104 28-29 -0.12897163623553354 1104 30-31 -0.04276509841119491 1104 32-33 -0.06526190160251844 1104 34-35 0.006534150471587452 1104 36-37 0.03088863958647181 1104 38-39 -0.08817581442357891 1104 40-41 -0.051340075406116625 1104 42-43 0.20011884555497517 1104 44-45 0.11872252423562202 1104 46-47 0.03290037686152658 1104 48-49 -0.2957488992992481 1104 50-51 -0.0032607172056415834 1104 52-53 -0.16764635360235047 1104 54-55 -0.08602242825600825 1104 56-57 -0.07359265031447393 1104 58-59 -0.1940891055159497 1104 60-61 -0.36392450143763 1104 62-63 -0.13001821757435295 1104 64-65 -0.056684112281310206 1104 66-67 -0.09720256826206253 1104 68-69 -0.13991261384995823 1104 70-71 -0.05915130544887148 1104 72-73 -0.17762441559405318 1104 74-75 -0.31537405532101204 1104 76-77 -0.37248791604084985 1104 78-79 -0.29704476151117376 1104 80-81 -0.27852269228570137 1104 82-83 -0.15495331114428978 1104 84-85 -0.11693108613001968 1104 86-87 -0.1869478844885073 1104 88-89 -0.16625443085595748 1104 90-91 -0.06019092978085894 1104 92-93 -0.11599650043442722 1104 94-95 -0.3277793966453686 1104 96-97 -0.5163113443511946 1104 98-99 -0.5367309345478297 1104 100-101 -0.13757879416046137 1105 1 -0.18256240788782918 1105 2 -0.18661870849030038 1105 3 -0.29211122702367476 1105 4 -0.2875919463466303 1105 5 -0.28985975084192006 1105 6 -0.07945155999031073 1105 7 -0.10617927800095828 1105 8 -0.10998630626863815 1105 9 -0.3066017684068214 1105 10-11 -0.189283168988176 1105 12-13 -0.1759716522203547 1105 14-15 -0.16402084496307623 1105 16-17 -0.2507132302639121 1105 18-19 -0.21153891447202255 1105 20-21 -0.34906760156516015 1105 22-23 -0.29318274868720806 1105 24-25 -0.27862731653789297 1105 26-27 -0.2277091859571243 1105 28-29 -0.3625556014286886 1105 30-31 -0.46371441938190117 1105 32-33 -0.40937425296585417 1105 34-35 -0.3665016279304254 1105 36-37 -0.34713161512871693 1105 38-39 -0.28754298657809585 1105 40-41 -0.37346449722797814 1105 42-43 -0.2915648936470845 1105 44-45 -0.2807378428414964 1105 46-47 -0.23088217535931221 1105 48-49 -0.5563461916303325 1105 50-51 -0.5430626053928549 1105 52-53 -0.4293815216172163 1105 54-55 -0.5670732340092783 1105 56-57 -0.5534844597216235 1105 58-59 -0.28922052883738303 1105 60-61 -0.41353957736641433 1105 62-63 -0.5042129923224934 1105 64-65 -0.43945915610668607 1105 66-67 -0.6642606712041612 1105 68-69 -0.41628440086920193 1105 70-71 -0.6105840783806258 1105 72-73 -0.4738060638505459 1105 74-75 -0.5255642468854198 1105 76-77 -0.5573544334548366 1105 78-79 -0.45432241373834614 1105 80-81 -0.6478689549845029 1105 82-83 -0.5668484681764134 1105 84-85 -0.5945511042937923 1105 86-87 -0.5085904912006534 1105 88-89 -0.5014208616752285 1105 90-91 -0.585679609105842 1105 92-93 -0.25557239753456074 1105 94-95 -0.32365822740550243 1105 96-97 -0.45026967328439227 1105 98-99 -0.6226511090045008 1105 100-101 -0.6388341535285313 1106 1 -0.17788419620045204 1106 2 -0.02978658431739234 1106 3 -0.19295215085166006 1106 4 -0.037747264332622876 1106 5 -0.1871835285827288 1106 6 -0.16227084454304475 1106 7 -0.15135729108501295 1106 8 -0.06911985257715969 1106 9 -0.13278142124141823 1106 10-11 -0.1372075096081602 1106 12-13 -0.3014634925093347 1106 14-15 -0.27848850317523244 1106 16-17 -0.11417938452267862 1106 18-19 -0.08490383195827889 1106 20-21 -0.3156207644504434 1106 22-23 -0.2140851546731497 1106 24-25 -0.1254880260100819 1106 26-27 0.0367608487051001 1106 28-29 -0.20435022861460084 1106 30-31 -0.0997042278060718 1106 32-33 0.0540946172358403 1106 34-35 -0.037463445167837506 1106 36-37 0.052463369882673305 1106 38-39 0.01704407127488139 1106 40-41 -0.17444868704523486 1106 42-43 -0.29946574136076975 1106 44-45 -0.09594848118993582 1106 46-47 0.03129458499606841 1106 48-49 -0.2275991239999584 1106 50-51 -0.21422132849229314 1106 52-53 -0.17604430674878557 1106 54-55 -0.21282176485389215 1106 56-57 -0.3536589590545205 1106 58-59 -0.4638582050766118 1106 60-61 -0.35275083359186254 1106 62-63 -0.3191129946989406 1106 64-65 -0.4775290373181278 1106 66-67 -0.15672308085662223 1106 68-69 0.19506925903363737 1106 70-71 0.16419380841208664 1106 72-73 0.08056032363982268 1106 74-75 0.16177979857946667 1106 76-77 0.010253873256598212 1106 78-79 0.01373300507702524 1106 80-81 -0.022529886485028072 1106 82-83 0.004282077015179908 1106 84-85 -0.0041322719455507695 1106 86-87 0.12477164244410588 1106 88-89 0.1114350541577025 1106 90-91 0.2581468828804283 1106 92-93 0.2156258764837098 1106 94-95 0.28681954090419737 1106 96-97 0.25324611483132387 1106 98-99 0.34416431211942466 1106 100-101 0.3331322230980618 1107 1 -0.31427299613292803 1107 2 -0.07431775092075554 1107 3 -0.11690807150088034 1107 4 -0.18087854662069702 1107 5 -0.33176955853663515 1107 6 -0.2021820701803705 1107 7 -0.15170235921735298 1107 8 -0.15700051361847045 1107 9 -0.3843360897153474 1107 10-11 -0.3130122755203928 1107 12-13 -0.2043925996269209 1107 14-15 -0.18153428800663818 1107 16-17 -0.25080278113505017 1107 18-19 -0.3244406959095798 1107 20-21 -0.28286659942843784 1107 22-23 -0.1501023236746093 1107 24-25 -0.23468595361528344 1107 26-27 -0.1623682886990494 1107 28-29 -0.500938770863101 1107 30-31 -0.5192599091418657 1107 32-33 -0.47392168785403754 1107 34-35 -0.38856654606426844 1107 36-37 -0.4924997525122521 1107 38-39 -0.4557538851273719 1107 40-41 -0.5401873935114097 1107 42-43 -0.4535051486379871 1107 44-45 -0.49814028445641156 1107 46-47 -0.6161550139553214 1107 48-49 -0.4558170084592277 1107 50-51 -0.42999317639419843 1107 52-53 -0.36363972801739664 1107 54-55 -0.47670703342365783 1107 56-57 -0.18742548943168202 1107 58-59 0.006731563529136508 1107 60-61 -0.08066336999158352 1107 62-63 -0.3469095981362216 1107 64-65 -0.2608957603376538 1107 66-67 -0.21074486235153955 1107 68-69 -0.40704608309272317 1107 70-71 -0.2483289375358524 1107 72-73 -0.1880367385642927 1107 74-75 -0.2267954672347159 1107 76-77 -0.3715863577156284 1107 78-79 -0.2302078219445356 1107 80-81 -0.20853029858078287 1107 82-83 -0.34057007742639556 1107 84-85 -0.5227771223622426 1107 86-87 -0.22609810302470024 1107 88-89 -0.3685336166348918 1107 90-91 -0.5762017948135245 1107 92-93 -0.5970579840094636 1107 94-95 -0.36384366018612013 1107 96-97 -0.12415663925922615 1107 98-99 -0.37369656336063173 1107 100-101 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-0.38743624751817407 2316 58-59 -0.24741627648617026 2316 60-61 -0.39762050499361834 2316 62-63 -0.34380170648209685 2316 64-65 -0.2646790207550609 2316 66-67 -0.14562257876838203 2316 68-69 -0.2540123027011276 2316 70-71 -0.14313875847347646 2316 72-73 -0.1542503702552196 2316 74-75 -0.041780286379321296 2316 76-77 -0.25718606127057697 2316 78-79 -0.0959648326309015 2316 80-81 -0.07057576942636601 2316 82-83 -0.16008524632835375 2316 84-85 -0.24594167327189354 2316 86-87 -0.03114364559086269 2316 88-89 0.009953796403912918 2316 90-91 -0.05681930897833709 2316 92-93 -0.13324101492103324 2316 94-95 0.06707149437172433 2316 96-97 -0.04288527250410468 2316 98-99 -0.018034223835421415 2316 100-101 -0.3775548518644243 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 1.0 4 5.0 5 12.0 6 20.0 7 48.0 8 146.0 9 302.0 10 479.0 11 796.0 12 1276.0 13 1805.0 14 2324.0 15 3255.0 16 4277.0 17 6011.0 18 5453.0 19 3272.0 20 2728.0 21 2819.0 22 2974.0 23 3141.0 24 3527.0 25 4052.0 26 4897.0 27 6066.0 28 7759.0 29 9455.0 30 12242.0 31 15675.0 32 19920.0 33 26417.0 34 36884.0 35 54571.0 36 86747.0 37 144594.0 38 138976.0 39 16148.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.03513715960578 20.68184601291681 15.487767834565588 21.795248992911816 2 15.5935301832055 21.4424353217025 40.21682629257243 22.747208202519573 3 18.990899336327146 25.75002980566705 30.285736994793943 24.97333386321186 4 13.02372531097246 18.00580216985256 37.598060644597226 31.372411874577754 5 13.059072669112926 38.281175475525764 34.58094590039874 14.078805954962576 6 30.622103882684893 38.15713547669197 17.359456344632992 13.861304295990145 7 27.627071493860033 33.67786035051464 21.097643365258513 17.59742479036681 8 25.988316178516076 36.06342645948416 19.57445455629297 18.37380280570679 9 26.179708301871795 17.48344791956444 20.625998489846204 35.710845288717564 10-11 24.205857807097722 27.964948535548224 27.482494138218815 20.34669951913524 12-13 25.31023858881583 25.312146281906166 28.09888845082603 21.27872667845197 14-15 22.457894527679528 26.879624846004052 26.36180105710766 24.300679569208757 16-17 21.253507133489645 30.14211342049835 27.172674164447802 21.431705281564202 18-19 21.27488773198744 28.264594841632558 30.01295552994476 20.447561896435243 20-21 21.915351905575648 27.329968604697374 30.532527917974804 20.222151571752175 22-23 21.69344172649062 27.305235681993047 30.284961244253406 20.716361347262936 24-25 21.516455985960274 27.563617519139306 30.358289671134624 20.5616368237658 26-27 21.275753560650077 27.85148081235042 30.14409515096648 20.728670476033024 28-29 21.034701749672728 27.993650849163153 30.3122672620519 20.659380139112216 30-31 21.31443972934499 27.75199442348305 30.276369827355293 20.657196019816666 32-33 21.165202877240393 27.80073918054286 30.161745419862495 20.87231252235425 34-35 21.615069745260897 27.79096292175019 29.97551961212892 20.618447720859994 36-37 21.688994511761365 27.702689276673198 29.85935643347945 20.748959778085993 38-39 21.426181284639345 27.96732962787219 29.884440798859906 20.722048288628557 40-41 21.69566426896634 28.000635854230417 29.702817629058536 20.600882247744703 42-43 21.381314064729473 28.196787771113783 29.53509723729238 20.88680092686436 44-45 21.522791400071533 28.224536025116244 29.526527043675237 20.726145531136986 46-47 21.883638675833563 28.495807336168184 28.723920041330526 20.896633946667727 48-49 21.68493422882804 28.647379088344 28.67805905496165 20.989627627866312 50-51 21.82267615149227 28.76509160275007 28.51480348130191 20.897428764455746 52-53 21.934656232265574 28.766772695696684 28.390743204683716 20.90782786735403 54-55 21.69757183165759 28.98342804911974 28.376743631522473 20.942256487700195 56-57 21.737471684616303 29.268529189683267 28.170647379088344 20.82335174661209 58-59 21.69900250367603 28.704526487302783 28.304971585264077 21.291499423757106 60-61 21.619441243095018 28.92016055319318 28.592536660970474 20.867861542741327 62-63 21.47549974168422 28.90577435122998 28.754917935063386 20.863807972022414 64-65 21.505225926956246 28.955053054087347 28.56901005444502 20.970710964511387 66-67 21.42192902277153 28.973492826769466 28.463458252195682 21.141119898263323 68-69 21.47549974168422 28.96443190398601 28.4747446647856 21.085323689544172 70-71 21.543695107896514 28.84854747049239 28.31013790088622 21.297619520724872 72-73 21.493303660135915 28.889083177681517 28.199419783014747 21.41819337916783 74-75 21.555776338274452 29.258991376227 27.905098756110164 21.280133529388387 76-77 21.696935977427177 28.935659500059614 28.129714263005205 21.237690259508007 78-79 21.705122600643804 29.053848905138498 27.86090688709613 21.380121607121566 80-81 21.460716130827006 29.367484004292017 27.902078448515677 21.269721416365297 82-83 21.554158615805623 29.436544378359603 27.772191961670618 21.237105044164156 84-85 21.441356660742617 29.528561027796414 27.723526961851864 21.30655534960911 86-87 21.390772165481064 29.24611532806104 27.81401263760283 21.549099868855066 88-89 21.38425465961928 29.154393355323293 27.74859913364861 21.712752851408816 90-91 21.51494749359518 29.30700766352659 27.694336494077675 21.48370834880055 92-93 21.17747582693156 29.267515290732565 27.816058150487038 21.73895073184884 94-95 21.173627945793427 29.314469657830944 27.718713984818983 21.79318841155665 96-97 21.3200333823471 29.59321225609029 27.295394030918413 21.7913603306442 98-99 21.01084863181974 29.791412817902106 27.257098028742632 21.940640521535514 100-101 20.96622389685133 29.77233523318945 27.15999332268142 22.1014475472778 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 330.0 1 206.0 2 93.0 3 125.5 4 133.5 5 137.5 6 216.0 7 427.0 8 632.5 9 758.5 10 818.5 11 857.0 12 916.5 13 986.5 14 1063.5 15 1085.0 16 1118.5 17 1173.0 18 1229.0 19 1286.5 20 1380.5 21 1637.0 22 1941.5 23 2243.5 24 2695.0 25 3467.5 26 4463.5 27 5584.0 28 6607.0 29 7746.0 30 9302.5 31 10616.0 32 12051.5 33 13592.5 34 15176.0 35 16723.0 36 18641.0 37 20447.0 38 21355.0 39 21808.5 40 21808.5 41 22257.5 42 22667.5 43 23207.0 44 23916.5 45 24351.0 46 24135.0 47 23601.0 48 23374.0 49 22537.5 50 21424.5 51 19783.0 52 18275.5 53 17808.5 54 17469.5 55 15949.0 56 13478.0 57 11732.5 58 10712.5 59 9320.5 60 7452.5 61 5838.0 62 4671.0 63 3526.0 64 2400.0 65 1684.0 66 1268.5 67 983.0 68 728.0 69 505.5 70 362.5 71 243.0 72 183.0 73 166.5 74 115.0 75 74.5 76 61.5 77 41.0 78 20.0 79 11.0 80 9.5 81 7.5 82 4.0 83 2.0 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04482772324444621 2 0.0 3 0.0 4 0.0 5 0.013829829511584468 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.006835432976990024 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0011127449032309344 24-25 4.768906728132576E-4 26-27 0.0018280809124508206 28-29 0.002304971585264078 30-31 0.0013511902396375632 32-33 0.0 34-35 0.0 36-37 3.97408894011048E-4 38-39 5.563724516154672E-4 40-41 0.0 42-43 0.011524857926320391 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 3.1792711520883837E-4 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.384453364066288E-4 84-85 1.5896355760441918E-4 86-87 0.0 88-89 0.0 90-91 0.008981441004649685 92-93 0.0027818622580773355 94-95 0.0 96-97 0.0 98-99 0.0015101537972419823 100-101 0.012717084608353536 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 629075.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.64714630893803 #Duplication Level Percentage of deduplicated Percentage of total 1 84.72615306921568 57.314824728667126 2 9.184941967529861 12.426702262331954 3 2.4619239260135544 4.996263840735421 4 1.1245985664548852 3.0430353505518237 5 0.6127011375494912 2.0723741747731594 6 0.37979446904884484 1.5415207209043587 7 0.27417912078470263 1.298320456900514 8 0.1963557768213076 1.062632637058894 9 0.1582302033007922 0.963343954186353 >10 0.814742635172028 9.838579460000346 >50 0.04660667233281065 2.1591943479196174 >100 0.018830910262911543 2.0841898431439274 >500 2.353863782795027E-4 0.11098482089111372 >1k 7.06159134838508E-4 1.088033401935409 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2891 0.4595636450343759 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2406 0.38246631959623256 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1536 0.24416802448038788 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 697 0.11079759965028017 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 1.5896355760441918E-4 0.0 0.0 0.0 0.0 7 1.5896355760441918E-4 0.0 0.0 0.0 0.0 8 1.5896355760441918E-4 0.0 0.0 0.0 0.0 9 1.5896355760441918E-4 0.0 0.0 1.5896355760441918E-4 0.0 10-11 1.5896355760441918E-4 0.0 0.0 2.384453364066288E-4 0.0 12-13 1.5896355760441918E-4 0.0 0.0 3.1792711520883837E-4 0.0 14-15 1.5896355760441918E-4 0.0 0.0 3.1792711520883837E-4 0.0 16-17 1.5896355760441918E-4 0.0 0.0 5.563724516154671E-4 0.0 18-19 1.5896355760441918E-4 0.0 0.0 6.358542304176767E-4 0.0 20-21 1.5896355760441918E-4 0.0 0.0 7.153360092198864E-4 0.0 22-23 1.5896355760441918E-4 0.0 0.0 0.0012717084608353535 0.0 24-25 1.5896355760441918E-4 0.0 0.0 0.001748599133648611 0.0 26-27 1.5896355760441918E-4 0.0 0.0 0.001748599133648611 0.0 28-29 1.5896355760441918E-4 0.0 0.0 0.0026228987004729165 0.0 30-31 1.5896355760441918E-4 0.0 0.0 0.004292016055319318 0.0 32-33 1.5896355760441918E-4 0.0 0.0 0.006438024082978977 0.0 34-35 1.5896355760441918E-4 0.0 0.0 0.008822477447045264 0.0 36-37 1.5896355760441918E-4 0.0 0.0 0.011445376147518182 0.0 38-39 1.5896355760441918E-4 0.0 0.0 0.014863092636013195 0.0 40-41 1.5896355760441918E-4 0.0 0.0 0.01915510869133251 0.0 42-43 1.5896355760441918E-4 0.0 0.0 0.02909033104160871 0.0 44-45 1.5896355760441918E-4 0.0 0.0 0.04498668680205063 0.0 46-47 3.1792711520883837E-4 0.0 0.0 0.06104200612009697 0.0 48-49 3.1792711520883837E-4 0.0 0.0 0.09823947859953105 0.0 50-51 3.1792711520883837E-4 0.0 0.0 0.13480109684854746 0.0 52-53 3.1792711520883837E-4 0.0 0.0 0.1697730795215197 0.0 54-55 3.1792711520883837E-4 0.0 0.0 0.23240472121766087 0.0 56-57 3.1792711520883837E-4 0.0 0.0 0.29447999046218654 0.0 58-59 3.1792711520883837E-4 0.0 0.0 0.3790486031077376 0.0 60-61 3.1792711520883837E-4 0.0 0.0 0.47108850296069626 0.0 62-63 3.1792711520883837E-4 0.0 0.0 0.5969081588045941 0.0 64-65 3.1792711520883837E-4 0.0 0.0 0.6897428764455749 0.0 66-67 3.1792711520883837E-4 0.0 0.0 0.8368636490084649 0.0 68-69 3.1792711520883837E-4 0.0 0.0 1.0222151571752176 0.0 70-71 3.1792711520883837E-4 0.0 0.0 1.1610698247426776 0.0 72-73 3.1792711520883837E-4 0.0 0.0 1.3755116639510394 0.0 74-75 3.1792711520883837E-4 0.0 0.0 1.6442395580813098 0.0 76-77 3.1792711520883837E-4 0.0 0.0 1.8683781743035408 0.0 78-79 3.1792711520883837E-4 0.0 0.0 2.090609227834519 0.0 80-81 3.1792711520883837E-4 0.0 0.0 2.413066804435083 0.0 82-83 3.1792711520883837E-4 0.0 0.0 2.790923180860788 0.0 84-85 3.1792711520883837E-4 0.0 0.0 3.202241386162222 0.0 86-87 3.1792711520883837E-4 0.0 0.0 3.6901800262289868 0.0 88-89 3.1792711520883837E-4 0.0 0.0 4.387155744545563 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1705 0.0 50.170597 1 GTACGAC 55 4.960713E-5 43.181854 3 GTATCAA 2815 0.0 37.646763 1 ATGGGCG 60 0.003949588 31.666693 5 ATCAACG 3500 0.0 30.128597 3 TCAACGC 3525 0.0 29.914919 4 TATCAAC 3575 0.0 29.496529 2 CAACGCA 3600 0.0 29.291693 5 AACGCAG 3685 0.0 28.742651 6 TCCGTGC 90 8.934684E-4 26.393106 8 ACGCAGA 4225 0.0 24.96257 7 GGACCGA 115 1.281536E-4 24.78066 6 CGCAGAG 4245 0.0 24.733044 8 GTGCATA 240 2.2737368E-10 23.761353 1 GTATAGC 220 2.1518645E-9 23.761353 1 GAGTACT 2700 0.0 22.962004 12-13 GCAGAGT 4560 0.0 22.711964 9 CAGCGTA 105 0.0021930852 22.622663 9 ATATCGT 55 0.0028788291 21.589212 92-93 GTACTTT 2900 0.0 20.96387 14-15 >>END_MODULE