FastQCFastQC Report
Thu 26 May 2016
SRR937465_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937465_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences690727
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40520.5866282916405469No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30500.4415637437077166No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20670.2992499207356886No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10860.15722564775953451No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8460.12247964825466502No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15150.059.6219441
GTATCAA29750.044.1049461
ATCAACG36950.035.245743
TCAACGC37800.034.4556774
TATCAAC38250.034.0503162
CAACGCA38650.033.7003675
AACGCAG39750.032.8634956
CGCAGAG44200.029.4473678
TGGTATC6700.029.0878182
ACGCAGA45300.028.732317
GCAGAGT47800.026.6313729
GTGGTAT8250.025.9312671
GTAGGAC1401.7693834E-523.7651463
TACATGG24000.023.5688172
GAGTACT32800.023.5306912-13
ACATGGG24350.022.6427693
GTACATG24900.022.5293061
CAGAGTA47400.022.34587910-11
GTCCGTG1302.9228904E-421.9418411
AGAGTAC46100.021.68812810-11
Produced by FastQC (version 0.11.5)