##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937465_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 690727 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.633958133966097 33.0 31.0 34.0 30.0 34.0 2 31.725241665665305 33.0 31.0 34.0 30.0 34.0 3 31.766076901583403 33.0 31.0 34.0 30.0 34.0 4 35.38187735530825 37.0 35.0 37.0 33.0 37.0 5 35.3573119336583 37.0 35.0 37.0 33.0 37.0 6 35.423287347968156 37.0 35.0 37.0 33.0 37.0 7 35.37288248468642 37.0 35.0 37.0 33.0 37.0 8 35.363994747563076 37.0 35.0 37.0 33.0 37.0 9 36.987055667434454 39.0 37.0 39.0 33.0 39.0 10-11 36.893199484021906 39.0 37.0 39.0 33.0 39.0 12-13 36.784041307202415 39.0 37.0 39.0 33.0 39.0 14-15 37.930444299991166 40.0 38.0 41.0 33.0 41.0 16-17 37.875415323275334 40.0 38.0 41.0 33.0 41.0 18-19 37.84565754632438 40.0 38.0 41.0 33.0 41.0 20-21 37.79532507054162 40.0 38.0 41.0 32.5 41.0 22-23 37.648256112762354 40.0 37.5 41.0 32.0 41.0 24-25 37.5229120911735 40.0 37.0 41.0 32.0 41.0 26-27 37.34415622959577 40.0 37.0 41.0 32.0 41.0 28-29 37.15230040232972 39.5 37.0 41.0 31.0 41.0 30-31 37.0997825479531 39.0 37.0 41.0 31.0 41.0 32-33 36.90857024555287 39.0 36.0 41.0 31.0 41.0 34-35 36.70072836301462 39.0 36.0 41.0 30.0 41.0 36-37 36.69364162686561 39.0 36.0 41.0 30.0 41.0 38-39 36.648603572757395 39.5 36.0 41.0 30.0 41.0 40-41 36.47408889474423 39.5 35.5 41.0 30.0 41.0 42-43 36.23024508959401 39.0 35.0 41.0 30.0 41.0 44-45 35.97470708398542 39.0 35.0 41.0 28.5 41.0 46-47 35.61174241053267 39.0 35.0 41.0 27.0 41.0 48-49 35.28408691132677 39.0 35.0 41.0 25.5 41.0 50-51 34.35681680316536 38.0 34.0 40.0 23.5 40.5 52-53 34.471901344525406 38.0 34.5 40.0 24.0 40.5 54-55 34.83436147711035 39.0 35.0 40.0 24.0 41.0 56-57 34.62970464452671 39.0 34.5 40.0 23.0 41.0 58-59 34.39779102308148 38.0 34.0 40.0 22.5 41.0 60-61 34.13445254058405 38.0 34.0 40.0 22.0 41.0 62-63 33.832103711017524 38.0 34.0 40.0 20.5 41.0 64-65 33.490444126261174 37.0 33.0 40.0 19.5 41.0 66-67 33.112945490765526 37.0 33.0 39.5 18.0 41.0 68-69 32.644345740068076 36.0 32.0 39.0 14.0 41.0 70-71 32.225760684032906 36.0 32.0 39.0 9.5 40.5 72-73 31.818083700217308 35.0 31.5 38.5 7.5 40.0 74-75 31.36314057507525 35.0 31.0 37.5 7.0 39.5 76-77 30.9122033741261 35.0 31.0 37.0 2.0 39.0 78-79 30.43941528273833 35.0 30.0 36.5 2.0 39.0 80-81 29.998214924275437 34.5 30.0 36.0 2.0 38.5 82-83 29.548763838680117 34.0 29.5 35.5 2.0 37.0 84-85 29.07686032252974 34.0 29.0 35.0 2.0 37.0 86-87 28.646372590039192 34.0 29.0 35.0 2.0 36.0 88-89 28.27132209975866 34.0 29.0 35.0 2.0 36.0 90-91 27.84723848930185 34.0 27.5 35.0 2.0 36.0 92-93 27.42304484984661 34.0 26.5 35.0 2.0 35.0 94-95 26.914634870216453 33.0 25.5 35.0 2.0 35.0 96-97 26.316847321734926 33.0 25.0 35.0 2.0 35.0 98-99 25.63956020251127 33.0 22.5 35.0 2.0 35.0 100-101 23.861580624472474 30.5 10.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.25429977683621274 1101 2 0.16509772540027257 1101 3 0.1892927303697789 1101 4 0.16163115930539362 1101 5 0.33462352595498857 1101 6 0.1347711573123931 1101 7 0.10296254374063096 1101 8 0.04218083545677587 1101 9 0.2984966369282134 1101 10-11 0.12949172599722658 1101 12-13 0.06678742284584871 1101 14-15 0.06947975473897117 1101 16-17 0.1725140932866296 1101 18-19 0.17251951180104186 1101 20-21 0.253800070065914 1101 22-23 0.19838938268748763 1101 24-25 0.22789098579199418 1101 26-27 0.29683187239790954 1101 28-29 0.12709423848348678 1101 30-31 0.08252242607344584 1101 32-33 0.025798449476830854 1101 34-35 -0.03650888478926362 1101 36-37 0.18416381842226315 1101 38-39 0.2687578669897164 1101 40-41 0.21259755622077137 1101 42-43 0.37581500899247544 1101 44-45 0.37292348385197016 1101 46-47 0.5255464485648105 1101 48-49 0.43203156521648367 1101 50-51 0.2473313425164676 1101 52-53 0.20807080899789554 1101 54-55 0.29265629741387755 1101 56-57 0.4738931408628275 1101 58-59 0.15936837548420613 1101 60-61 0.4271128073835442 1101 62-63 0.4554293580150883 1101 64-65 0.35704935850334607 1101 66-67 0.40922883381501407 1101 68-69 0.3496410951795923 1101 70-71 0.4662737563262631 1101 72-73 0.43105837493979493 1101 74-75 0.21266907918667854 1101 76-77 0.40379079693042996 1101 78-79 0.48097427553533123 1101 80-81 0.4194588911000956 1101 82-83 0.34554254873157575 1101 84-85 0.23341954368817142 1101 86-87 0.42286964739890465 1101 88-89 0.391606320937985 1101 90-91 0.3230607201947926 1101 92-93 0.30059737533732545 1101 94-95 0.13525803522773217 1101 96-97 0.4866622364408961 1101 98-99 0.559105875379867 1101 100-101 0.9278736687862477 1102 1 0.15432416265185722 1102 2 0.11765129409529607 1102 3 0.06984673338151381 1102 4 -0.11451239304935257 1102 5 0.10554726777150591 1102 6 -0.06273080957274146 1102 7 -0.056087263842826474 1102 8 0.191837654051227 1102 9 0.3748687395551187 1102 10-11 0.24402496012597652 1102 12-13 0.2583878443466716 1102 14-15 0.2579923445837906 1102 16-17 0.37010817447536937 1102 18-19 0.30575061457390973 1102 20-21 0.30878474438177506 1102 22-23 0.35346937528274225 1102 24-25 0.48551733813677345 1102 26-27 0.2957184908150765 1102 28-29 0.34259498429784685 1102 30-31 0.3093376563503085 1102 32-33 0.16649259082911527 1102 34-35 0.1538406511682453 1102 36-37 0.2286243223008455 1102 38-39 0.36429686471489475 1102 40-41 0.36150722546367575 1102 42-43 0.27255442964245447 1102 44-45 0.15576557056579787 1102 46-47 0.238659787427963 1102 48-49 0.2943384811762044 1102 50-51 0.3248515364638038 1102 52-53 0.46324433988601044 1102 54-55 0.5923477367465892 1102 56-57 0.5117926143428093 1102 58-59 0.4676455799085346 1102 60-61 0.6657582732238225 1102 62-63 0.51353790871444 1102 64-65 0.39294278714902475 1102 66-67 0.44658318641610606 1102 68-69 0.37922091155618176 1102 70-71 0.3588221986600395 1102 72-73 0.49545250312018396 1102 74-75 0.34119300754577964 1102 76-77 0.4065546710355399 1102 78-79 0.5133139610473094 1102 80-81 0.5705966584985696 1102 82-83 0.7262834503303601 1102 84-85 0.630987993070697 1102 86-87 0.4962162178988301 1102 88-89 0.6970551267450169 1102 90-91 0.7367411627706453 1102 92-93 0.5865887032428851 1102 94-95 0.4990054052976127 1102 96-97 1.1371351788654493 1102 98-99 1.536654460112569 1102 100-101 1.5327340110420593 1103 1 0.07601860505696578 1103 2 -0.005636276567653198 1103 3 0.18650483484249492 1103 4 0.02938306249723155 1103 5 0.03563683535097795 1103 6 0.1845381134138293 1103 7 0.04727886199228237 1103 8 0.13756484001407898 1103 9 0.0828050144247996 1103 10-11 0.18737163070816365 1103 12-13 0.16997106143791996 1103 14-15 0.11816046733542862 1103 16-17 0.03246593088092453 1103 18-19 0.0736549534623947 1103 20-21 -0.07378628564539014 1103 22-23 0.07632746206051166 1103 24-25 0.08568803045010753 1103 26-27 -0.13973228372424273 1103 28-29 -0.21335355105198772 1103 30-31 -0.10791327970431297 1103 32-33 0.006689712920007196 1103 34-35 0.034607481797429784 1103 36-37 0.18768019191394103 1103 38-39 0.12867172705979613 1103 40-41 0.07045113560243266 1103 42-43 0.0681733269333975 1103 44-45 -0.05468703937473407 1103 46-47 0.1531539508520936 1103 48-49 0.2456948426768264 1103 50-51 0.07756058866261384 1103 52-53 0.2966413004615944 1103 54-55 0.36623731900927936 1103 56-57 0.2695637031028113 1103 58-59 0.12339589192674794 1103 60-61 0.3693886968344131 1103 62-63 0.18268297882676876 1103 64-65 0.09054208235495764 1103 66-67 0.44091546745274 1103 68-69 0.41386410955927033 1103 70-71 0.5547035583252615 1103 72-73 0.4424663053373479 1103 74-75 0.4746799027810802 1103 76-77 0.5370021418526605 1103 78-79 0.40405252649771484 1103 80-81 0.2994399886505441 1103 82-83 0.6337755581051177 1103 84-85 0.4995677596076611 1103 86-87 0.6474808093385214 1103 88-89 0.617020621202105 1103 90-91 0.9129837293957124 1103 92-93 0.7984343827084182 1103 94-95 0.639819222197989 1103 96-97 0.7952274628387279 1103 98-99 0.7160704354447738 1103 100-101 1.0253813937599219 1104 1 0.17975385572547964 1104 2 0.2600265317424615 1104 3 0.19814535319617832 1104 4 0.19711872455755497 1104 5 0.10235710761755712 1104 6 0.29452277971896734 1104 7 0.152634435416374 1104 8 0.2600380527971424 1104 9 0.09507945016353148 1104 10-11 0.15186991969220998 1104 12-13 0.27775150910701285 1104 14-15 0.41796552371479834 1104 16-17 0.4054963925747401 1104 18-19 0.31417190467506373 1104 20-21 0.2576505937607081 1104 22-23 0.26899954538605897 1104 24-25 0.37848259633825165 1104 26-27 0.45966418578601775 1104 28-29 0.6049309450865437 1104 30-31 0.3404617226331439 1104 32-33 0.23828179923749104 1104 34-35 0.3250762814683128 1104 36-37 0.4987746811336109 1104 38-39 0.6304790460941518 1104 40-41 0.5906130743798528 1104 42-43 0.654236026785668 1104 44-45 0.9146857751062569 1104 46-47 0.9998586089293937 1104 48-49 1.1755693681737753 1104 50-51 1.0076884941172963 1104 52-53 1.0295657352653436 1104 54-55 1.0026197824982503 1104 56-57 1.1288172139579444 1104 58-59 0.9880769697732958 1104 60-61 1.0425509909047648 1104 62-63 1.1417873601495785 1104 64-65 1.087391195057613 1104 66-67 1.0852770621570045 1104 68-69 1.201532172464809 1104 70-71 1.182067658779662 1104 72-73 1.3591678779760556 1104 74-75 1.2841152675173184 1104 76-77 1.4133153232203561 1104 78-79 1.3914583271222796 1104 80-81 1.5056683672058107 1104 82-83 1.4280955461373566 1104 84-85 1.480043478140864 1104 86-87 1.6474808093385214 1104 88-89 1.496327145502267 1104 90-91 1.8407573517006277 1104 92-93 1.9798750020400924 1104 94-95 1.7883237194584929 1104 96-97 1.7301270458370261 1104 98-99 1.8762252683636014 1104 100-101 2.080772963967675 1105 1 0.05380363115090603 1105 2 -0.016781399008440445 1105 3 0.16286974853177583 1105 4 0.0921841463992763 1105 5 0.07766313001175718 1105 6 0.09015332818700728 1105 7 0.16645288848766882 1105 8 -0.004220587525061603 1105 9 0.1616940148262458 1105 10-11 0.22675842316099448 1105 12-13 0.11382393177112249 1105 14-15 0.1732688575401653 1105 16-17 0.2944189617922035 1105 18-19 0.1651217338718638 1105 20-21 0.13412469388090642 1105 22-23 0.14588286149848528 1105 24-25 0.03405715931402398 1105 26-27 0.05924403100880227 1105 28-29 -0.12339971725029386 1105 30-31 0.1970094420941635 1105 32-33 0.24569513243024943 1105 34-35 0.3907445753341534 1105 36-37 0.3510734074900199 1105 38-39 0.29458652031551935 1105 40-41 0.45930350412913157 1105 42-43 0.19890646623488095 1105 44-45 0.5638446747962789 1105 46-47 0.36206352532017405 1105 48-49 0.5949195516290544 1105 50-51 0.45381761395365317 1105 52-53 0.46911972278194014 1105 54-55 0.8411369577085921 1105 56-57 0.9228003550498229 1105 58-59 0.8546007293414419 1105 60-61 0.9788352042611521 1105 62-63 0.946588037036328 1105 64-65 0.9670098663774382 1105 66-67 1.0188134000099467 1105 68-69 1.2272422709402377 1105 70-71 1.0428709609748061 1105 72-73 0.9520533185850439 1105 74-75 0.8749809866831235 1105 76-77 0.8396617163207445 1105 78-79 0.9153055373367351 1105 80-81 0.6739707995698545 1105 82-83 0.8481297331352273 1105 84-85 0.7833443553523409 1105 86-87 0.8389701710406499 1105 88-89 0.8530929816998949 1105 90-91 0.9078878843535598 1105 92-93 1.0807055589428636 1105 94-95 0.9018274518246407 1105 96-97 0.9028649578206611 1105 98-99 1.0632527919281145 1105 100-101 0.7984870742095396 1106 1 0.04729467258427533 1106 2 -7.889577990312091E-4 1106 3 -0.12506418203933478 1106 4 -0.2575149017530194 1106 5 -0.11484106931635552 1106 6 0.014565422251962445 1106 7 -0.0350448718706744 1106 8 0.037142794333838935 1106 9 0.27591573935032443 1106 10-11 -0.044623155786375435 1106 12-13 -0.15808256318968716 1106 14-15 -0.3657149050578212 1106 16-17 -0.5445543024855155 1106 18-19 -0.3363655281662119 1106 20-21 -0.31947273053118863 1106 22-23 -0.18047492125626974 1106 24-25 -0.45290742635226877 1106 26-27 -0.1318778894839241 1106 28-29 0.059201066624282817 1106 30-31 0.10714382059359906 1106 32-33 0.053943312721393966 1106 34-35 0.1540774420754758 1106 36-37 -0.3792947336074093 1106 38-39 -0.21298976747843312 1106 40-41 0.041820301441561014 1106 42-43 -0.219609071279109 1106 44-45 -0.4166284495038397 1106 46-47 -0.3264513123058066 1106 48-49 -0.03244257043142085 1106 50-51 -0.17422689892428167 1106 52-53 -0.33919844631100204 1106 54-55 -0.08999406132836185 1106 56-57 -0.5095729652189362 1106 58-59 -0.5351319134357198 1106 60-61 -0.34638526046448703 1106 62-63 -0.2420842753936867 1106 64-65 -0.17835687494395813 1106 66-67 -0.26665342529800284 1106 68-69 -0.2034863124864188 1106 70-71 -0.2710288150610296 1106 72-73 -0.32303766685728874 1106 74-75 -0.0988956930481173 1106 76-77 -0.40888601604207864 1106 78-79 -0.20054342682900028 1106 80-81 -0.11623776705948785 1106 82-83 -0.41534311120967615 1106 84-85 -0.6030638193397095 1106 86-87 -0.3442955064509512 1106 88-89 -0.40721286936393497 1106 90-91 -0.3248948704056822 1106 92-93 -0.22826000656665713 1106 94-95 -0.32995796250906295 1106 96-97 -0.2436565987302899 1106 98-99 0.13194680088668065 1106 100-101 0.2867114583080834 1107 1 0.017703649572201385 1107 2 0.015081684716346189 1107 3 0.036419071549183 1107 4 0.08492500483162502 1107 5 0.12458562241297244 1107 6 0.168230835785792 1107 7 -0.13871313343367575 1107 8 0.060582074352069526 1107 9 0.12937039872603862 1107 10-11 0.05062205952463472 1107 12-13 0.1311917589793694 1107 14-15 0.26120751462775615 1107 16-17 0.3636715177468872 1107 18-19 0.515917879215877 1107 20-21 0.2892579028704958 1107 22-23 0.2912441491470119 1107 24-25 0.13869587166549735 1107 26-27 0.3814900474037515 1107 28-29 0.19688903654909495 1107 30-31 0.2623186895842622 1107 32-33 0.1796375198300737 1107 34-35 0.3771991668419403 1107 36-37 0.391559959944658 1107 38-39 0.7630832275090427 1107 40-41 0.558815915476643 1107 42-43 0.5823140604137507 1107 44-45 0.631019078406851 1107 46-47 0.696119318817459 1107 48-49 0.8957479342393526 1107 50-51 0.8737339121944245 1107 52-53 0.8306117325452291 1107 54-55 0.7678892833970892 1107 56-57 0.9387175052754841 1107 58-59 1.02047585598784 1107 60-61 1.173056098615028 1107 62-63 1.083256776098679 1107 64-65 0.8580622979883827 1107 66-67 0.8351386970533241 1107 68-69 1.0453780358845108 1107 70-71 1.1452818479601596 1107 72-73 1.2713317794858483 1107 74-75 1.0941828840765133 1107 76-77 1.325097379947902 1107 78-79 1.260235651548765 1107 80-81 0.9607587013843499 1107 82-83 0.9756770528368008 1107 84-85 1.1454552054951073 1107 86-87 1.6961821080398174 1107 88-89 1.389121005094939 1107 90-91 1.3383043207540375 1107 92-93 1.5356597359715067 1107 94-95 1.447621639906064 1107 96-97 1.567597388510542 1107 98-99 2.194703123511424 1107 100-101 2.3326038882465667 1108 1 0.007720487415124211 1108 2 -0.17313843828638653 1108 3 -0.10683485431343698 1108 4 -0.013075310559990783 1108 5 -0.037240800283015574 1108 6 0.006908022289067617 1108 7 -0.10994127857486546 1108 8 -0.020351484842365153 1108 9 0.13401225119397253 1108 10-11 0.015022230446540163 1108 12-13 0.06061525362025577 1108 14-15 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90-91 -0.8222145585148404 2316 92-93 -1.198106497794729 2316 94-95 -1.5547082061737818 2316 96-97 -2.0224699515568005 2316 98-99 -2.1295663570080556 2316 100-101 -1.8647566216139424 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3240.0 3 673.0 4 585.0 5 644.0 6 779.0 7 848.0 8 1033.0 9 1328.0 10 1668.0 11 2224.0 12 2763.0 13 3717.0 14 4111.0 15 5184.0 16 6287.0 17 8286.0 18 7038.0 19 4767.0 20 4345.0 21 4636.0 22 5030.0 23 5500.0 24 5981.0 25 6773.0 26 7590.0 27 8973.0 28 10446.0 29 12537.0 30 14988.0 31 18667.0 32 23598.0 33 30986.0 34 42545.0 35 60734.0 36 90305.0 37 135945.0 38 125782.0 39 20183.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.807643023387584 19.227561839509296 15.62542676743463 22.339368369668495 2 14.649785132224647 19.87908150742924 42.06049410148381 23.410639258862297 3 18.262304224566623 25.089318910628805 30.82141446298276 25.826962401821817 4 12.001661431125402 16.89997865119096 38.82201047689173 32.27634944079191 5 12.822649969796943 36.95358603224757 35.61187328655732 14.611890711398171 6 30.14168909654157 37.08220579379992 18.399994196043153 14.376110913615358 7 26.96504158123323 32.83270428204715 22.210371582843948 17.99188255387567 8 25.271528175809504 34.92334815825177 20.73684439639849 19.068279269540234 9 25.484895577137102 16.86581955949847 21.403603470266365 36.24568139309807 10-11 23.58574143231432 26.923498310515438 28.638160076213637 20.852600180956603 12-13 24.884292093988414 24.491403985474385 29.046221490217704 21.578082430319498 14-15 22.035320872974708 25.668532214246593 27.661198182088565 24.63494873069013 16-17 20.58559986766105 28.977079386684405 28.5387791586464 21.898541587008147 18-19 20.56302164379686 26.9614169486853 31.647532926355957 20.82802848116188 20-21 21.123986439674617 26.088754404457816 32.0437772456115 20.74348191025607 22-23 20.93846892687336 25.987081216837943 31.98465241596044 21.08979744032825 24-25 20.702354018979836 26.167452838742484 32.06776916933661 21.062423972941076 26-27 20.49453224246619 26.319455175851182 31.95429617811983 21.231716403562803 28-29 20.538131818063096 26.4226698512106 31.982828420603575 21.05636991012273 30-31 20.66036668011412 26.16753397900456 32.0515685631445 21.120530777736825 32-33 20.465333617763537 26.26444462825988 31.876862038290692 21.39335971568589 34-35 20.851920826528904 26.35659983270349 31.68955688190071 21.101922458866895 36-37 20.829554934890638 26.112807863905115 31.725271137872248 21.332366063332003 38-39 20.559902973750106 26.414482560518092 31.679549338602463 21.34606512712934 40-41 20.828413011121135 26.398846396003005 31.615089117069335 21.15765147580652 42-43 20.559766019428743 26.33971576467311 31.507802253919987 21.592715961978158 44-45 20.79856349983676 26.511843871295387 31.37534008053107 21.31425254833678 46-47 21.034224349873735 26.780624507150357 30.698683969029272 21.486467173946636 48-49 20.955126163454207 26.97182316874539 30.482373247064746 21.590677420735656 50-51 21.09075183556368 27.035736079356433 30.37425255063355 21.49925953444634 52-53 21.17988633197102 27.13410563347576 30.094162177224597 21.591845857328625 54-55 20.989865230127556 27.056432389757983 30.31832334755795 21.635379032556507 56-57 20.985551816177324 27.353704885691076 30.09189683184403 21.56884646628757 58-59 21.097908107578895 27.024465242096024 30.179817897664435 21.697808752660645 60-61 20.99788073821677 27.026317430891904 30.420762673141947 21.555039157749377 62-63 20.823617555054465 27.036541963821403 30.66303251228812 21.476807968836013 64-65 20.821073320737884 26.964291542313816 30.506271735307024 21.708363401641275 66-67 20.775415906543888 27.092335423595422 30.206330266245473 21.92591840361522 68-69 20.840456202063425 26.98388061866512 30.397853166760346 21.777810012511107 70-71 20.83726813672372 26.95220646366708 30.354560237669375 21.855965161939825 72-73 20.8387196931117 27.003181760806456 30.144318170277163 22.01378037580469 74-75 20.842279192029384 27.27147441290989 29.95472428379939 21.931522111261337 76-77 20.858853870065616 27.094195354978183 30.028981512044194 22.017969262912008 78-79 20.96069710605521 27.003066378720625 29.93944863217166 22.096787883052507 80-81 20.777565744284708 27.207330314575984 29.838044611169984 22.177059329969325 82-83 20.973841184266554 27.218341710547598 29.799113700644376 22.008703404541468 84-85 20.872415806326448 27.173986357850765 29.681284291221083 22.272313544601705 86-87 20.812117941621043 27.060540386852693 29.918199252057708 22.20914241946855 88-89 20.91263937419346 26.79132697361692 29.88713691621699 22.408896735972633 90-91 20.78477312375101 26.813287248434115 30.101152243374692 22.30078738444018 92-93 20.761166107753766 26.75477252136092 30.083804292712223 22.40025707817309 94-95 20.54001865685175 26.715059387109363 30.11195561816417 22.632966337874716 96-97 20.615100506357944 26.870481602182977 29.931767523604286 22.582650367854793 98-99 20.599506867082614 26.97345656578406 29.86660457167148 22.560431995461844 100-101 20.569380874088267 26.805170466866496 30.000268343255993 22.625180315789244 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1079.0 1 651.5 2 237.5 3 247.0 4 192.5 5 171.5 6 323.5 7 687.0 8 1202.5 9 1419.0 10 1211.0 11 1029.5 12 1025.0 13 1079.5 14 1099.5 15 1182.0 16 1268.0 17 1292.5 18 1358.0 19 1425.5 20 1622.0 21 1919.5 22 2200.5 23 2613.5 24 3183.0 25 3987.0 26 5160.0 27 6407.0 28 7341.0 29 8533.0 30 10135.0 31 11590.0 32 13053.5 33 14502.5 34 16255.5 35 17966.0 36 20037.5 37 21975.0 38 22821.5 39 23434.0 40 23725.0 41 24212.0 42 24738.0 43 25164.5 44 25866.0 45 26460.5 46 26590.0 47 26219.5 48 25749.5 49 24863.5 50 23556.0 51 21836.0 52 20343.5 53 19611.5 54 18735.0 55 16916.5 56 14743.5 57 13046.0 58 11719.0 59 10161.5 60 8220.0 61 6639.5 62 5255.0 63 3855.0 64 2717.5 65 1984.0 66 1445.0 67 1043.0 68 787.5 69 573.5 70 441.5 71 300.0 72 193.0 73 166.5 74 127.0 75 94.5 76 73.0 77 48.0 78 36.5 79 31.5 80 15.5 81 8.0 82 6.5 83 4.0 84 3.5 85 4.0 86 3.5 87 3.0 88 2.0 89 1.5 90 1.5 91 2.0 92 2.0 93 1.5 94 1.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3493420700218755 2 0.3132930955355734 3 0.3160438204963755 4 0.3132930955355734 5 0.29751262076044516 6 0.22324304681878657 7 0.2307713467115083 8 0.22758629675689526 9 0.22555944678577786 10-11 0.23330490917540503 12-13 0.23156760920016156 14-15 0.21347073445804202 16-17 0.22983030922491807 18-19 0.21709010940646595 20-21 0.21868263438377245 22-23 0.21687294690956052 24-25 0.20507378457769856 26-27 0.2244736343012507 28-29 0.22657287177133656 30-31 0.23525937164755395 32-33 0.23598324663723874 34-35 0.22172290934044853 36-37 0.21745204690130832 38-39 0.20688347205191052 40-41 0.2039879720931714 42-43 0.2083312220312801 44-45 0.22469079679815615 46-47 0.2165110094147181 48-49 0.22606615927855722 50-51 0.23648995913001808 52-53 0.24655182148663654 54-55 0.24083320906812677 56-57 0.19790742217981921 58-59 0.18523960986033555 60-61 0.19341939724377358 62-63 0.19421565973242683 64-65 0.1917544847674986 66-67 0.20550810957150945 68-69 0.19392610973655294 70-71 0.19776264718188227 72-73 0.21513564693431703 74-75 0.20196112212205403 76-77 0.21636623441678116 78-79 0.2139050594518529 80-81 0.19841413467259855 82-83 0.21086478449517682 84-85 0.1910306097778138 86-87 0.19819697217569315 88-89 0.20854838452818553 90-91 0.20550810957150945 92-93 0.20883793452405944 94-95 0.20789689703746922 96-97 0.2015991846272116 98-99 0.21144388448692464 100-101 0.18987240979431816 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 690727.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.43106620445322 #Duplication Level Percentage of deduplicated Percentage of total 1 85.86526553045255 59.61716935707806 2 8.536510080168403 11.853979930623094 3 2.29398270934305 4.778209960928048 4 1.009560653745163 2.803794903503659 5 0.5526400024694991 1.9185192299344491 6 0.36262480367288075 1.510645605071318 7 0.23744399358315255 1.154019274682514 8 0.18773538635275042 1.0427734431021134 9 0.13778982950272242 0.8610205297053456 >10 0.7605611563788235 9.377540655074661 >50 0.03962073281535369 1.8412225398189497 >100 0.015013958322869243 1.5525044365621985 >500 4.170543976098445E-4 0.20385398169880456 >1k 8.34108795219689E-4 1.4847461522167904 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4052 0.5866282916405469 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3050 0.4415637437077166 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2067 0.2992499207356886 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1086 0.15722564775953451 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 846 0.12247964825466502 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 7.238749896847814E-5 0.0 16-17 0.0 0.0 0.0 1.447749979369563E-4 0.0 18-19 0.0 0.0 0.0 1.447749979369563E-4 0.0 20-21 0.0 0.0 0.0 2.1716249690543443E-4 0.0 22-23 0.0 0.0 0.0 7.962624886532595E-4 0.0 24-25 1.447749979369563E-4 0.0 0.0 0.0013029749814326066 0.0 26-27 1.447749979369563E-4 0.0 0.0 0.0013029749814326066 0.0 28-29 1.447749979369563E-4 0.0 0.0 0.002533562463896735 0.0 30-31 1.447749979369563E-4 0.0 0.0 0.003546987449455429 0.0 32-33 1.447749979369563E-4 0.0 0.0 0.005935774915415208 0.0 34-35 1.447749979369563E-4 0.0 0.0 0.009120824870028245 0.0 36-37 1.447749979369563E-4 0.0 0.0 0.011943937329798893 0.0 38-39 1.447749979369563E-4 0.0 0.0 0.014332724795758672 0.0 40-41 1.447749979369563E-4 0.0 0.0 0.01780732474624562 0.0 42-43 1.447749979369563E-4 0.0 0.0 0.027434862109053215 0.0 44-45 1.447749979369563E-4 0.0 0.0 0.042781011890370584 0.0 46-47 1.447749979369563E-4 0.0 0.0 0.06087788663249012 0.0 48-49 1.447749979369563E-4 0.0 0.0 0.09743357361157157 0.0 50-51 1.447749979369563E-4 0.0 0.0 0.1348579105782748 0.0 52-53 1.447749979369563E-4 0.0 0.0 0.17061733506870297 0.0 54-55 1.447749979369563E-4 0.0 0.0 0.23084373421047677 0.0 56-57 1.447749979369563E-4 0.0 0.0 0.29193878333987233 0.0 58-59 1.447749979369563E-4 0.0 0.0 0.37221651969591457 0.0 60-61 1.447749979369563E-4 0.0 0.0 0.4658859433611253 0.0 62-63 1.447749979369563E-4 0.0 0.0 0.6038565163950447 0.0 64-65 1.447749979369563E-4 0.0 0.0 0.7068639274271891 0.0 66-67 1.447749979369563E-4 0.0 0.0 0.8559097878032855 0.0 68-69 1.447749979369563E-4 0.0 0.0 1.044913547609982 0.0 70-71 1.447749979369563E-4 0.0 0.0 1.1837527706315232 0.0 72-73 2.1716249690543443E-4 0.0 0.0 1.389333267702001 0.0 74-75 2.895499958739126E-4 0.0 0.0 1.6434133890813594 0.0 76-77 2.895499958739126E-4 0.0 0.0 1.8521789361064502 0.0 78-79 2.895499958739126E-4 0.0 0.0 2.053343795739851 0.0 80-81 4.343249938108688E-4 0.0 0.0 2.3593257538796077 0.0 82-83 4.343249938108688E-4 0.0 0.0 2.710694673872601 0.0 84-85 4.343249938108688E-4 0.0 0.0 3.1170780930816373 0.0 86-87 4.343249938108688E-4 0.0 0.0 3.596790048745742 0.0 88-89 4.343249938108688E-4 7.238749896847814E-5 0.0 4.259280439305254 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1515 0.0 59.621944 1 GTATCAA 2975 0.0 44.104946 1 ATCAACG 3695 0.0 35.24574 3 TCAACGC 3780 0.0 34.455677 4 TATCAAC 3825 0.0 34.050316 2 CAACGCA 3865 0.0 33.700367 5 AACGCAG 3975 0.0 32.863495 6 CGCAGAG 4420 0.0 29.447367 8 TGGTATC 670 0.0 29.087818 2 ACGCAGA 4530 0.0 28.73231 7 GCAGAGT 4780 0.0 26.631372 9 GTGGTAT 825 0.0 25.931267 1 GTAGGAC 140 1.7693834E-5 23.765146 3 TACATGG 2400 0.0 23.568817 2 GAGTACT 3280 0.0 23.53069 12-13 ACATGGG 2435 0.0 22.642769 3 GTACATG 2490 0.0 22.529306 1 CAGAGTA 4740 0.0 22.345879 10-11 GTCCGTG 130 2.9228904E-4 21.941841 1 AGAGTAC 4610 0.0 21.688128 10-11 >>END_MODULE