FastQCFastQC Report
Thu 26 May 2016
SRR937465_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937465_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences690727
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30550.44228761869740146No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26590.3849567195143668No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16310.23612802163517568No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7160.1036588985228607No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19900.042.7534981
TGTACGC500.001614885638.0023162
GTATCAA31900.036.6535571
GTGTACG753.0656476E-431.6869531
TCAACGC40750.028.443454
ATCAACG41000.028.2700163
CAACGCA41450.027.9631065
TATCAAC41650.027.8288292
ACCGCGA350.00839261627.14254612-13
AACGCAG42950.027.0931876
ACGCAGA48950.023.5799147
CGCAGAG49050.023.5318418
ATCGCGG550.00287543121.59378886-87
GCAGAGT52000.021.5574469
GTACATG25850.021.3289321
GAGTACT32450.021.29790912-13
TACATGG26300.020.409992
GTACTTT33700.020.36550514-15
AGAGTAC46600.020.18217310-11
AGTACTT33950.019.86722612-13
Produced by FastQC (version 0.11.5)