##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937465_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 690727 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.18705074508453 33.0 31.0 34.0 31.0 34.0 2 32.40628207671048 34.0 31.0 34.0 31.0 34.0 3 32.486551126566646 34.0 31.0 34.0 31.0 34.0 4 36.09184091544127 37.0 35.0 37.0 35.0 37.0 5 35.984322315473406 37.0 35.0 37.0 35.0 37.0 6 36.06712492779347 37.0 35.0 37.0 35.0 37.0 7 36.02445539265151 37.0 35.0 37.0 35.0 37.0 8 35.996625294798086 37.0 35.0 37.0 35.0 37.0 9 37.70812346990924 39.0 37.0 39.0 35.0 39.0 10-11 37.62964383902758 39.0 37.0 39.0 35.0 39.0 12-13 37.560070766018995 39.0 37.0 39.0 35.0 39.0 14-15 38.790985439978456 40.0 38.0 41.0 35.0 41.0 16-17 38.765591181465325 40.0 38.0 41.0 35.0 41.0 18-19 38.764257079859334 40.0 38.0 41.0 35.0 41.0 20-21 38.66117945295319 40.0 38.0 41.0 34.0 41.0 22-23 38.53372171639447 40.0 38.0 41.0 34.0 41.0 24-25 38.39728575833868 40.0 38.0 41.0 34.0 41.0 26-27 38.35787510840028 40.0 38.0 41.0 34.0 41.0 28-29 38.2633754001219 40.0 38.0 41.0 34.0 41.0 30-31 38.33735614794267 40.0 38.0 41.0 34.0 41.0 32-33 38.26175464402 40.0 38.0 41.0 34.0 41.0 34-35 38.157389967382194 40.0 38.0 41.0 34.0 41.0 36-37 38.00484706693093 40.0 37.5 41.0 33.0 41.0 38-39 37.871485405956335 40.0 37.0 41.0 33.0 41.0 40-41 37.706044500938866 40.0 37.0 41.0 33.0 41.0 42-43 37.50551303192144 40.0 37.0 41.0 33.0 41.0 44-45 37.222765289325594 40.0 37.0 41.0 32.0 41.0 46-47 36.88440947002216 40.0 36.5 41.0 31.0 41.0 48-49 36.58984736371968 40.0 36.0 41.0 30.5 41.0 50-51 36.374595896787014 39.0 36.0 41.0 30.0 41.0 52-53 36.15777434500171 39.0 35.5 41.0 30.0 41.0 54-55 35.94085000296789 39.0 35.0 40.5 29.0 41.0 56-57 35.696906303069085 39.0 35.0 40.0 28.5 41.0 58-59 35.46386416051494 38.5 35.0 40.0 28.0 41.0 60-61 35.150015128987285 38.0 34.5 40.0 28.0 41.0 62-63 34.79508184854508 38.0 34.0 40.0 27.0 41.0 64-65 34.453591650536325 37.0 34.0 40.0 26.0 41.0 66-67 34.03711162297116 37.0 34.0 39.5 26.0 41.0 68-69 33.64448038081615 36.0 33.0 39.0 25.5 40.5 70-71 33.24671252173435 36.0 33.0 39.0 25.0 40.0 72-73 32.79792233400461 35.0 32.0 38.0 23.5 40.0 74-75 32.33408785236425 35.0 32.0 37.5 22.5 39.5 76-77 31.0107719837215 34.0 30.5 36.0 21.0 38.5 78-79 31.631530257250695 35.0 31.5 36.0 22.0 39.0 80-81 31.47256079464101 35.0 32.0 36.0 21.0 38.0 82-83 31.13746386054114 34.5 31.0 36.0 20.0 37.0 84-85 30.764140535985995 34.0 31.0 35.0 18.5 37.0 86-87 30.382594715423025 34.0 31.0 35.0 17.5 36.0 88-89 29.997555474159835 34.0 30.5 35.0 12.5 36.0 90-91 29.557598009054228 34.0 30.0 35.0 7.0 35.5 92-93 29.188134385944085 34.0 29.5 35.0 2.0 35.0 94-95 28.779947794135744 34.0 29.0 35.0 2.0 35.0 96-97 28.310662533823056 34.0 29.0 35.0 2.0 35.0 98-99 27.828170174323574 34.0 29.0 35.0 2.0 35.0 100-101 25.951340399318397 31.5 23.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.19543397090075842 1101 2 0.287319799695787 1101 3 -8.365978057014445E-4 1101 4 0.032806568298227035 1101 5 0.035446158441040154 1101 6 0.07232683482716595 1101 7 0.08356817459559096 1101 8 0.12222142456983676 1101 9 0.18503891104042225 1101 10-11 0.10738489290211106 1101 12-13 0.11836286301152654 1101 14-15 0.13354249942190677 1101 16-17 0.017852099342597683 1101 18-19 0.0779934704069305 1101 20-21 0.1660706720897167 1101 22-23 0.21686986410265519 1101 24-25 0.24830906145649578 1101 26-27 0.20270290428771887 1101 28-29 -0.0340885309450627 1101 30-31 -0.05071522612444568 1101 32-33 0.05077029857679349 1101 34-35 0.06920070142187029 1101 36-37 -0.048167767847878906 1101 38-39 -0.038044257343628374 1101 40-41 0.05863412908839649 1101 42-43 -0.053094724435815976 1101 44-45 -0.12425839481250733 1101 46-47 0.023992413816706915 1101 48-49 0.12009161571400284 1101 50-51 -0.13828504444894207 1101 52-53 -0.10046063155579787 1101 54-55 -0.172070299050894 1101 56-57 0.07470664674529104 1101 58-59 -0.20904765668985448 1101 60-61 -0.05457390995045586 1101 62-63 -0.22147311944996773 1101 64-65 -0.25874866478412173 1101 66-67 -0.14540712147269375 1101 68-69 -0.20854515951148045 1101 70-71 -0.19524440646287644 1101 72-73 -0.3961528547919997 1101 74-75 -0.17207253251955734 1101 76-77 -0.24537702289672225 1101 78-79 -0.17004962951072855 1101 80-81 -0.10810291793508853 1101 82-83 -0.12096867303404935 1101 84-85 -0.19998505124399557 1101 86-87 -0.19112309140425054 1101 88-89 -0.4157821957812118 1101 90-91 -0.41423007922793786 1101 92-93 -0.3227608225041614 1101 94-95 -0.30491222288156905 1101 96-97 -0.3369134372681657 1101 98-99 -0.09820338007943974 1101 100-101 -0.042925561028724246 1102 1 0.11018151540932308 1102 2 0.200277567735057 1102 3 0.20575665034934332 1102 4 0.13708262997119647 1102 5 0.023530928520180794 1102 6 0.18370148283482735 1102 7 0.0636563551289484 1102 8 0.1375604586067425 1102 9 0.27047737512562975 1102 10-11 0.2942416502650218 1102 12-13 0.1186196260703909 1102 14-15 0.21191441693020607 1102 16-17 0.19100652029518272 1102 18-19 0.24873734458324037 1102 20-21 0.17273583170111095 1102 22-23 0.31071208965543207 1102 24-25 0.25418079206620803 1102 26-27 0.30576857511125155 1102 28-29 0.18268713023093142 1102 30-31 0.36245881027983984 1102 32-33 0.34278821592133113 1102 34-35 0.2287880649413907 1102 36-37 0.29066780055521946 1102 38-39 0.17494626244803868 1102 40-41 0.25600704694913645 1102 42-43 0.2796150202785128 1102 44-45 0.3072143482535594 1102 46-47 0.25322308583282904 1102 48-49 0.3272909492870397 1102 50-51 0.23220446965033403 1102 52-53 0.20563519935971897 1102 54-55 0.3691079096418477 1102 56-57 0.31398133782862203 1102 58-59 0.38654207065180657 1102 60-61 0.36815687420482135 1102 62-63 0.2796464920857602 1102 64-65 0.28218969072191413 1102 66-67 0.5309152305303186 1102 68-69 0.544355328560826 1102 70-71 0.5386751951049291 1102 72-73 0.5884591615411594 1102 74-75 0.5939415614138923 1102 76-77 0.4641180483205396 1102 78-79 0.5117577510254776 1102 80-81 0.57222084381727 1102 82-83 0.5864731837055146 1102 84-85 0.48864101274296345 1102 86-87 0.5426376463645752 1102 88-89 0.7379979616407972 1102 90-91 0.7459896245111004 1102 92-93 0.7575579552858791 1102 94-95 0.7177465608180249 1102 96-97 0.5722000048342082 1102 98-99 0.6994263898394983 1102 100-101 1.1035090369257183 1103 1 0.1004832553696744 1103 2 0.05391166214809573 1103 3 0.09719633906529168 1103 4 0.1522907644693987 1103 5 0.07069706903849493 1103 6 -0.046725798568886034 1103 7 -0.06095270441146994 1103 8 -0.07218552186458993 1103 9 0.046206029409454175 1103 10-11 -0.04176708624478209 1103 12-13 0.012669255319352146 1103 14-15 0.039416068791304326 1103 16-17 0.14792598288970282 1103 18-19 0.11045087516102114 1103 20-21 0.00293001715004948 1103 22-23 0.033226956405293606 1103 24-25 -0.01672442781485728 1103 26-27 0.06023410627636849 1103 28-29 -0.05199277579652062 1103 30-31 -0.04247841899848481 1103 32-33 0.0401232133517766 1103 34-35 -0.01206429538456888 1103 36-37 -0.04736721890002826 1103 38-39 0.007107998003434091 1103 40-41 0.028723807833067383 1103 42-43 0.3343558616264346 1103 44-45 0.20949024576475495 1103 46-47 -0.04608129710528175 1103 48-49 0.06659423098442119 1103 50-51 0.1463664254538699 1103 52-53 0.34603421558466607 1103 54-55 0.19819718355712013 1103 56-57 -0.07783827893959483 1103 58-59 0.24538319832230826 1103 60-61 0.08648151997238784 1103 62-63 -0.21222084511414607 1103 64-65 -0.030841657930906763 1103 66-67 -0.08455720174337245 1103 68-69 0.04536479923111614 1103 70-71 -0.10591220670109891 1103 72-73 0.13117628950532634 1103 74-75 0.04065986403484345 1103 76-77 0.03386696543381973 1103 78-79 0.1807578244415211 1103 80-81 0.12897695570332957 1103 82-83 -0.0902392456315404 1103 84-85 0.041458353613677446 1103 86-87 -0.12703601929874253 1103 88-89 -0.10397032262614303 1103 90-91 -0.0827454169689652 1103 92-93 0.09107421694067952 1103 94-95 -0.06497497210907355 1103 96-97 -0.005349367458570953 1103 98-99 0.2407486128774572 1103 100-101 0.7274757060395842 1104 1 0.14875073484683554 1104 2 0.18122433862583875 1104 3 0.04769187920404505 1104 4 0.10049653517456392 1104 5 0.13295467511213843 1104 6 0.09778118574212158 1104 7 0.06686579700035367 1104 8 0.008697848556337817 1104 9 0.259229385244673 1104 10-11 0.1436196220597239 1104 12-13 0.18913624112632732 1104 14-15 0.03680653572101278 1104 16-17 0.06772982379326464 1104 18-19 0.15424680612199637 1104 20-21 0.045517952150142094 1104 22-23 0.07554888919883496 1104 24-25 0.13116391670708794 1104 26-27 -0.0770496792762998 1104 28-29 0.08593147201094808 1104 30-31 0.14592126684777185 1104 32-33 0.13712893543790727 1104 34-35 0.20610987294947591 1104 36-37 0.31400542728430736 1104 38-39 0.04575779137254443 1104 40-41 0.38670527650184283 1104 42-43 0.37609109359532056 1104 44-45 0.4232377446895228 1104 46-47 0.4499057627522305 1104 48-49 0.30652261140965464 1104 50-51 0.331708254651474 1104 52-53 0.31957424213813823 1104 54-55 0.09345262047392566 1104 56-57 0.18615512009176172 1104 58-59 0.23761490541134123 1104 60-61 0.30419941738622214 1104 62-63 0.4291357193902172 1104 64-65 0.4078230951405146 1104 66-67 0.3866701483323638 1104 68-69 0.28186366790363593 1104 70-71 0.3080783873860753 1104 72-73 0.22432007094407425 1104 74-75 0.3383696271105592 1104 76-77 0.18767654712314297 1104 78-79 0.3724285426942302 1104 80-81 0.3550947259596988 1104 82-83 0.48981152385814397 1104 84-85 0.548779725418381 1104 86-87 0.5674681600680884 1104 88-89 0.6756293183410023 1104 90-91 0.48219885813995944 1104 92-93 0.5762052078339615 1104 94-95 0.3808105992580586 1104 96-97 0.6687016264266461 1104 98-99 0.9703248793097536 1104 100-101 1.3818883507279551 1105 1 -0.01692441223369201 1105 2 0.07464630686909146 1105 3 0.07645165821423205 1105 4 0.08566591501135434 1105 5 -0.08230875191694054 1105 6 0.07007970123039087 1105 7 0.007789768490980009 1105 8 -0.05163152748482247 1105 9 0.030318835511423003 1105 10-11 0.06854001933530895 1105 12-13 -0.03526831995162638 1105 14-15 -0.08719974814967912 1105 16-17 -0.10970649402720056 1105 18-19 0.05895549178085702 1105 20-21 0.013676203420622812 1105 22-23 0.027594178404491743 1105 24-25 0.047067723931427 1105 26-27 -0.1344091092997104 1105 28-29 -0.14394379948178937 1105 30-31 0.0014396861801628802 1105 32-33 0.04107915474077117 1105 34-35 -0.2873453070264773 1105 36-37 -0.018776793368111555 1105 38-39 -0.008698906854057498 1105 40-41 -0.09223163174942783 1105 42-43 -0.3203259930504032 1105 44-45 -0.3414656956242439 1105 46-47 -0.40491810963037267 1105 48-49 -0.2098835891681219 1105 50-51 -0.20027648903830197 1105 52-53 -0.16592132435912532 1105 54-55 -0.12176467914861888 1105 56-57 -0.1600567654911842 1105 58-59 -0.4173375965391912 1105 60-61 -0.4191211496382081 1105 62-63 -0.3336053292570682 1105 64-65 -0.31636203127494156 1105 66-67 -0.42309845023795134 1105 68-69 -0.4454772320816147 1105 70-71 -0.4798383407838003 1105 72-73 -0.4460040597519992 1105 74-75 -0.1929561536287423 1105 76-77 -0.17957141272756516 1105 78-79 -0.19397120004663293 1105 80-81 -0.1790494393187494 1105 82-83 -5.036976588357334E-4 1105 84-85 0.1880159610002714 1105 86-87 0.1918118329694245 1105 88-89 0.26069748146859695 1105 90-91 0.14520520527110037 1105 92-93 0.13606869706913827 1105 94-95 -0.039691451434645586 1105 96-97 0.12372279231661665 1105 98-99 0.2750069428654136 1105 100-101 0.7431496321736653 1106 1 -0.2340996641933799 1106 2 -0.11026410746461579 1106 3 -0.16004050303996564 1106 4 -0.12888475688114198 1106 5 -0.12297224575792853 1106 6 -0.19755747682112457 1106 7 -0.1363871631999487 1106 8 -0.07014356555873036 1106 9 -0.19305042540683104 1106 10-11 -0.3870345887273956 1106 12-13 -0.4534864050580083 1106 14-15 -0.3211723125393817 1106 16-17 -0.37830601810335196 1106 18-19 -0.3237782708171295 1106 20-21 -0.18070019635541001 1106 22-23 -0.24775926504455015 1106 24-25 -0.15294277438884052 1106 26-27 -0.23710017872860334 1106 28-29 -0.30736765166543734 1106 30-31 -0.21391585693294246 1106 32-33 -0.19039130998486797 1106 34-35 -0.5759266340141593 1106 36-37 -0.377136955742138 1106 38-39 -0.23255808938484535 1106 40-41 -0.3739015645601782 1106 42-43 -0.7228840837558863 1106 44-45 -0.37808733616176227 1106 46-47 -0.4696227568868139 1106 48-49 -0.41190626553990484 1106 50-51 -0.7328912706732424 1106 52-53 -0.7596013355573419 1106 54-55 -0.6648427866514197 1106 56-57 -0.5976092570925289 1106 58-59 -0.7465824173678541 1106 60-61 -0.463161603165652 1106 62-63 -0.36461036845079065 1106 64-65 -0.48171057550786145 1106 66-67 -0.7192735622200388 1106 68-69 -0.3226993765384947 1106 70-71 -0.08630880550944653 1106 72-73 -0.42498992201404207 1106 74-75 -0.5583501905828285 1106 76-77 -0.49847538809150294 1106 78-79 -0.6820556065415957 1106 80-81 -0.797732949957048 1106 82-83 -0.566468143347528 1106 84-85 -0.5981332551251519 1106 86-87 -0.3927696339958615 1106 88-89 -0.32325408628055996 1106 90-91 -0.5463191508319234 1106 92-93 -0.5351764037147326 1106 94-95 -1.0462529016026174 1106 96-97 -0.6035308471010765 1106 98-99 -0.30841970887031067 1106 100-101 0.12108916184891783 1107 1 0.10034440051384053 1107 2 0.15047296248298636 1107 3 -4.22708553756479E-4 1107 4 -0.015127352005322336 1107 5 -0.06871729018718042 1107 6 -0.11438078509179661 1107 7 0.09804835741977769 1107 8 -0.022802200783154092 1107 9 0.2159479227362553 1107 10-11 0.08447443952505296 1107 12-13 -0.053729911551513965 1107 14-15 -0.002093365170956929 1107 16-17 0.03127973605477763 1107 18-19 0.030011031985331726 1107 20-21 0.20504755370154726 1107 22-23 0.1081268992297666 1107 24-25 0.13812936618987948 1107 26-27 0.1545145114058215 1107 28-29 0.16006513484629892 1107 30-31 -0.010096649824255621 1107 32-33 0.010732949527550772 1107 34-35 0.5388382611783626 1107 36-37 0.1317453352125213 1107 38-39 0.2204850297766896 1107 40-41 0.21527734863189352 1107 42-43 0.2991418617897921 1107 44-45 0.4540320247404921 1107 46-47 0.30839473001226025 1107 48-49 0.0971504672010397 1107 50-51 -0.31193832011502565 1107 52-53 -0.15345528862182078 1107 54-55 0.07626424228455875 1107 56-57 -0.20826587021624476 1107 58-59 0.04906081001879414 1107 60-61 0.1721304622250983 1107 62-63 0.37527200794474425 1107 64-65 0.21930332287445253 1107 66-67 0.4465564958797543 1107 68-69 -0.01162695115685608 1107 70-71 0.10196372240124418 1107 72-73 -0.059712381572019524 1107 74-75 0.10617689417451714 1107 76-77 0.17024897964602204 1107 78-79 0.22717414397104818 1107 80-81 0.28309767774857875 1107 82-83 -0.0918013627596963 1107 84-85 0.0651228966178401 1107 86-87 0.12153459247941711 1107 88-89 0.10848577473561605 1107 90-91 0.3601586915011801 1107 92-93 0.2923647503066853 1107 94-95 0.2899605012226303 1107 96-97 0.4219376536963715 1107 98-99 0.584594075590843 1107 100-101 1.1643151244827763 1108 1 -0.09216911808663042 1108 2 -0.1694282487225749 1108 3 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-0.344659659189567 2316 70-71 -0.34974082305330256 2316 72-73 -0.2766285672382125 2316 74-75 -0.35967207167444926 2316 76-77 -0.07961573896869822 2316 78-79 -0.4896518541049488 2316 80-81 -0.3665986906977885 2316 82-83 -0.4048575195658515 2316 84-85 -0.43828920054425424 2316 86-87 -0.5054645657697421 2316 88-89 -0.3559051584053172 2316 90-91 -0.21164566155317388 2316 92-93 -0.441822358880426 2316 94-95 -0.39508940257727687 2316 96-97 -0.2223173968086769 2316 98-99 -0.4815434905467235 2316 100-101 -1.3965765301588782 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 3.0 4 5.0 5 12.0 6 42.0 7 71.0 8 147.0 9 335.0 10 537.0 11 926.0 12 1407.0 13 1975.0 14 2548.0 15 3528.0 16 4812.0 17 6699.0 18 5948.0 19 3601.0 20 3079.0 21 3008.0 22 3167.0 23 3518.0 24 3989.0 25 4857.0 26 5790.0 27 7230.0 28 8871.0 29 11111.0 30 14220.0 31 17958.0 32 22768.0 33 30339.0 34 41986.0 35 61150.0 36 96422.0 37 157462.0 38 145031.0 39 16172.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.05353508577521 20.687592253795533 15.545715150698703 21.713157509730557 2 15.555928753328017 21.38572837025337 40.229497326729664 22.82884554968895 3 18.927448905283853 25.839441631787956 30.307198068122425 24.925911394805762 4 13.086212063521478 18.042005017901428 37.558543389790756 31.313239528786337 5 13.231720914338007 38.22759405616581 34.52848111346978 14.012203916026401 6 30.704894987455244 37.97694313382856 17.391530952170683 13.926630926545508 7 27.616989056457907 33.67495407013191 21.070697974742554 17.637358898667635 8 25.952076580182908 36.0841548108008 19.62584349533173 18.337925113684566 9 26.111184302915625 17.527909000227297 20.6289894560369 35.731917240820174 10-11 24.230339917217655 27.891554840045345 27.467581258586964 20.410523984150032 12-13 25.302409284453663 25.339907311825776 28.131990160749265 21.225693242971293 14-15 22.426732992919053 26.77200978099886 26.517205784629816 24.284051441452267 16-17 21.25977412204822 30.155546257783467 27.13394727584125 21.450732344327065 18-19 21.311386408812744 28.227939547751863 30.053190334242036 20.407483709193357 20-21 21.902937050383148 27.44506874640777 30.454868566018124 20.19712563719096 22-23 21.741623095203796 27.24071209391312 30.32638964795609 20.69127516292699 24-25 21.52868362951971 27.616417532303018 30.219913858626807 20.63498497955047 26-27 21.33828759472926 27.784618480611133 30.208036993362708 20.669056931296904 28-29 21.183851814745267 27.92575101020265 30.24201429558001 20.648382879472074 30-31 21.35431219570105 27.797306316388386 30.216858469409768 20.631523018500797 32-33 21.283155284215038 27.835888853338588 30.03509345949992 20.845862402946462 34-35 21.603759517146425 27.858690915513655 29.939759123358435 20.597790443981488 36-37 21.772711939738855 27.595200419268394 29.929335323506972 20.70275231748578 38-39 21.440380931974573 27.888804115084543 29.915364536206056 20.75545041673483 40-41 21.672672416164417 27.94591785177067 29.782605863097867 20.598803868967046 42-43 21.440403344212353 28.105733059572042 29.503522890826584 20.950340705389024 44-45 21.52080344332855 28.2319932476941 29.50876395450011 20.73843935447724 46-47 21.952522487176555 28.393344982894835 28.683908403754305 20.970224126174305 48-49 21.68772901594986 28.646773616783477 28.565916780435685 21.099580586830978 50-51 21.79196701446447 28.759480952677396 28.52610365600302 20.92244837685511 52-53 21.942533012318904 28.67522190387809 28.386902495486638 20.99534258831637 54-55 21.640387591624478 28.879571813466104 28.405795632717414 21.074244962192008 56-57 21.787478989528424 29.195326083966606 28.193410710743898 20.823784215761073 58-59 21.713209415586764 28.68586286622645 28.270575784644297 21.330351933542484 60-61 21.537307793093362 28.947833224993374 28.5984911549715 20.91636782694176 62-63 21.485695506328838 28.87378081354862 28.811817114431605 20.828706565690933 64-65 21.54130469874835 28.82754606924738 28.64997940574164 20.98116982626264 66-67 21.483813431355657 28.94392430004908 28.347161758552947 21.22510051004232 68-69 21.473896343996977 28.922714762851314 28.492226306485772 21.111162586665934 70-71 21.64697485403061 28.888475475839225 28.171911623550262 21.29263804657991 72-73 21.527349484455485 28.834878084797733 28.203658179944004 21.43411425080278 74-75 21.59507301727021 29.31954303219651 27.90791441481222 21.17746953572106 76-77 21.64545471655227 28.93017067524507 28.14483869893605 21.279535909266613 78-79 21.702640840737367 28.987501574428105 27.982039213755943 21.32781837107859 80-81 21.545415192977835 29.351538306740576 27.84189701575297 21.26114948452862 82-83 21.704384309518485 29.271800841001006 27.689331998503018 21.334482850977487 84-85 21.4489486717874 29.40244367664826 27.720144805105967 21.428462846458377 86-87 21.402667045011995 29.24107498331468 27.707111492673665 21.64914647899966 88-89 21.40809610743463 29.09282538542724 27.775952004192682 21.723126502945448 90-91 21.594876795651704 29.1892627575158 27.668619142662504 21.547241304169987 92-93 21.290434198467977 29.131154257652508 27.802253008088652 21.776158535790863 94-95 21.20374619784662 29.228624333492103 27.67779455559143 21.88983491306985 96-97 21.348521195783572 29.39547751861444 27.40264967201224 21.853351613589737 98-99 21.138902300693545 29.58015944082064 27.341936352521586 21.93900190596423 100-101 20.98015452423563 29.51873345727706 27.32032503373702 22.180786984750288 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 323.0 1 214.0 2 121.0 3 149.5 4 146.0 5 151.5 6 233.5 7 435.5 8 657.5 9 829.0 10 895.5 11 900.0 12 981.0 13 1072.5 14 1110.5 15 1134.5 16 1192.0 17 1282.0 18 1346.0 19 1400.5 20 1519.0 21 1760.0 22 2048.5 23 2455.0 24 3034.5 25 3744.0 26 4788.5 27 6115.0 28 7302.5 29 8622.5 30 10215.0 31 11557.5 32 13194.5 33 14959.0 34 16688.0 35 18176.0 36 20192.5 37 22348.0 38 23275.5 39 23861.0 40 24172.0 41 24666.5 42 25079.5 43 25387.0 44 26023.5 45 26651.5 46 26481.5 47 25836.5 48 25465.5 49 24498.0 50 23378.0 51 21897.5 52 20336.5 53 19791.5 54 19352.0 55 17533.5 56 14896.0 57 13065.0 58 11904.0 59 10333.5 60 8307.0 61 6668.5 62 5307.0 63 3849.0 64 2619.0 65 1836.0 66 1337.0 67 1061.5 68 790.0 69 526.0 70 380.5 71 271.5 72 191.0 73 167.5 74 142.0 75 93.5 76 57.0 77 35.0 78 23.5 79 14.5 80 10.5 81 7.5 82 4.5 83 3.0 84 2.0 85 2.0 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05443539922429556 2 0.0 3 0.0 4 0.0 5 0.01867597473386736 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.003474599950486951 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 5.067124927793469E-4 24-25 2.895499958739126E-4 26-27 0.0012305874824641283 28-29 0.003474599950486951 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.014622274791632585 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 7.238749896847814E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 1.447749979369563E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.619374948423907E-4 84-85 7.962624886532595E-4 86-87 0.0 88-89 0.0 90-91 0.009482762364870636 92-93 8.686499876217377E-4 94-95 0.0 96-97 0.0 98-99 7.238749896847814E-5 100-101 0.013174524812263022 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 690727.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.81896258605896 #Duplication Level Percentage of deduplicated Percentage of total 1 84.21733177918254 56.273147412509125 2 9.457027132401578 12.63817484270571 3 2.6034501549970215 5.218795155042461 4 1.1500735668508315 3.0738689053848427 5 0.6299256364055307 2.104548876549026 6 0.3864470535337139 1.549319472693717 7 0.273721122559483 1.2802833013121306 8 0.1888913801072084 1.009722084817008 9 0.16432713781844005 0.988215198638805 >10 0.8592859133866008 10.311633834250882 >50 0.04909804531529706 2.2170673576399413 >100 0.0195520954231505 2.1650791860027443 >500 2.1724550463829247E-4 0.10393541380433542 >1k 6.517365139148774E-4 1.066208958649223 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3055 0.44228761869740146 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2659 0.3849567195143668 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1631 0.23612802163517568 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 716 0.1036588985228607 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 1.447749979369563E-4 0.0 8 0.0 0.0 0.0 1.447749979369563E-4 0.0 9 0.0 0.0 0.0 1.447749979369563E-4 0.0 10-11 0.0 0.0 0.0 1.447749979369563E-4 0.0 12-13 0.0 0.0 0.0 1.447749979369563E-4 0.0 14-15 0.0 0.0 0.0 1.447749979369563E-4 0.0 16-17 0.0 0.0 0.0 2.895499958739126E-4 0.0 18-19 0.0 0.0 0.0 3.619374948423907E-4 0.0 20-21 0.0 0.0 0.0 4.343249938108688E-4 0.0 22-23 0.0 0.0 0.0 7.962624886532595E-4 0.0 24-25 0.0 0.0 0.0 0.0011581999834956503 0.0 26-27 0.0 0.0 0.0 0.0013029749814326066 0.0 28-29 0.0 0.0 0.0 0.002605949962865213 0.0 30-31 0.0 0.0 0.0 0.0036917624473923848 0.0 32-33 0.0 0.0 0.0 0.006080549913352164 0.0 34-35 0.0 0.0 0.0 0.009265599867965203 0.0 36-37 0.0 0.0 0.0 0.01208871232773585 0.0 38-39 0.0 0.0 0.0 0.014549887292664106 0.0 40-41 0.0 0.0 0.0 0.018096874742119535 0.0 42-43 1.447749979369563E-4 0.0 0.0 0.027434862109053215 0.0 44-45 1.447749979369563E-4 0.0 0.0 0.04292578688830754 0.0 46-47 1.447749979369563E-4 0.0 0.0 0.060588336636616204 0.0 48-49 1.447749979369563E-4 0.0 0.0 0.09656492362394983 0.0 50-51 1.447749979369563E-4 0.0 0.0 0.13384448559271608 0.0 52-53 1.447749979369563E-4 0.0 0.0 0.16974868508108124 0.0 54-55 1.447749979369563E-4 0.0 0.0 0.22968553422698113 0.0 56-57 1.447749979369563E-4 0.0 0.0 0.2910701333522506 0.0 58-59 1.447749979369563E-4 0.0 0.0 0.37272323218869396 0.0 60-61 1.447749979369563E-4 0.0 0.0 0.4663202683549362 0.0 62-63 1.447749979369563E-4 0.0 0.0 0.6051594913764773 0.0 64-65 1.447749979369563E-4 0.0 0.0 0.7095422648890227 0.0 66-67 1.447749979369563E-4 0.0 0.0 0.8589500627599616 0.0 68-69 1.447749979369563E-4 0.0 0.0 1.04976351004087 0.0 70-71 1.447749979369563E-4 0.0 0.0 1.1892542205531274 0.0 72-73 1.447749979369563E-4 0.0 0.0 1.397802605081313 0.0 74-75 1.447749979369563E-4 0.0 0.0 1.6566603013925907 0.0 76-77 1.447749979369563E-4 0.0 0.0 1.8669459858960198 0.0 78-79 1.447749979369563E-4 0.0 0.0 2.0684003955252943 0.0 80-81 1.447749979369563E-4 0.0 0.0 2.378146503611412 0.0 82-83 1.447749979369563E-4 0.0 0.0 2.7332071860517972 0.0 84-85 1.447749979369563E-4 0.0 0.0 3.1468293551576814 0.0 86-87 1.447749979369563E-4 0.0 0.0 3.636386010681499 0.0 88-89 1.447749979369563E-4 0.0 0.0 4.309951688583189 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1990 0.0 42.753498 1 TGTACGC 50 0.0016148856 38.002316 2 GTATCAA 3190 0.0 36.653557 1 GTGTACG 75 3.0656476E-4 31.686953 1 TCAACGC 4075 0.0 28.44345 4 ATCAACG 4100 0.0 28.270016 3 CAACGCA 4145 0.0 27.963106 5 TATCAAC 4165 0.0 27.828829 2 ACCGCGA 35 0.008392616 27.142546 12-13 AACGCAG 4295 0.0 27.093187 6 ACGCAGA 4895 0.0 23.579914 7 CGCAGAG 4905 0.0 23.531841 8 ATCGCGG 55 0.002875431 21.593788 86-87 GCAGAGT 5200 0.0 21.557446 9 GTACATG 2585 0.0 21.328932 1 GAGTACT 3245 0.0 21.297909 12-13 TACATGG 2630 0.0 20.40999 2 GTACTTT 3370 0.0 20.365505 14-15 AGAGTAC 4660 0.0 20.182173 10-11 AGTACTT 3395 0.0 19.867226 12-13 >>END_MODULE