FastQCFastQC Report
Thu 26 May 2016
SRR937464_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937464_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences857758
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43460.5066697133690388No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33770.39370078740157477No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22710.2647599905800937No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12610.147011161656318No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9160.10679002702393915No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20550.052.020261
GTATCAA37700.044.613241
CCATGCG704.303254E-640.7220549
ATCAACG47600.034.8272173
TCAACGC47800.034.685554
TATCAAC48700.034.3331762
CAACGCA49050.033.609885
AACGCAG50250.032.7127086
CGCAGAG57250.028.6299138
ACGCAGA57350.028.5799927
GCAGAGT60100.026.5608069
GTAGTAC1806.692062E-926.3892963
GGACCGA1501.0578206E-625.3381656
GTACATG32500.025.1447281
TACATGG32750.024.3667892
GAGTACT39500.024.17549712-13
ACATGGG32600.023.1675343
ACCGAGT2503.8198777E-1022.804358
GTACTTT42600.022.13743814-15
AGTACTT41400.022.09056512-13
Produced by FastQC (version 0.11.5)