##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937464_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 857758 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.717927434078142 33.0 31.0 34.0 30.0 34.0 2 31.81493031834154 33.0 31.0 34.0 30.0 34.0 3 31.846815768550105 33.0 31.0 34.0 30.0 34.0 4 35.45093371323846 37.0 35.0 37.0 33.0 37.0 5 35.46159989181098 37.0 35.0 37.0 33.0 37.0 6 35.482261896712124 37.0 35.0 37.0 33.0 37.0 7 35.37149056027457 37.0 35.0 37.0 33.0 37.0 8 35.383777242532275 37.0 35.0 37.0 33.0 37.0 9 37.009470037003446 39.0 37.0 39.0 33.0 39.0 10-11 36.906073158163494 39.0 37.0 39.0 33.0 39.0 12-13 36.7566085072946 39.0 37.0 39.0 33.0 39.0 14-15 37.92893450133954 40.0 38.0 41.0 33.0 41.0 16-17 37.85490837742114 40.0 38.0 41.0 33.0 41.0 18-19 37.837040284089454 40.0 38.0 41.0 33.0 41.0 20-21 37.7347591045493 40.0 38.0 41.0 32.0 41.0 22-23 37.577797001018936 40.0 37.0 41.0 32.0 41.0 24-25 37.514004532746995 40.0 37.0 41.0 32.0 41.0 26-27 37.523394127481176 40.0 37.0 41.0 32.0 41.0 28-29 37.43501430473397 40.0 37.0 41.0 32.0 41.0 30-31 37.20225459861639 39.5 37.0 41.0 31.5 41.0 32-33 36.9463671571702 39.0 36.0 41.0 31.0 41.0 34-35 36.963605702307646 39.5 36.0 41.0 31.0 41.0 36-37 36.82934114283982 40.0 36.0 41.0 30.5 41.0 38-39 36.69923568185899 40.0 36.0 41.0 30.0 41.0 40-41 36.48102961441339 39.5 35.5 41.0 30.0 41.0 42-43 36.210797217863316 39.0 35.0 41.0 30.0 41.0 44-45 35.96513060793371 39.0 35.0 41.0 28.5 41.0 46-47 35.60078658549381 39.0 35.0 41.0 27.0 41.0 48-49 35.256178316028524 39.0 35.0 40.5 25.5 41.0 50-51 34.302488580695254 38.0 34.0 39.5 23.5 40.5 52-53 34.408939351192295 38.0 34.0 39.5 24.0 40.5 54-55 34.79323480515483 39.0 35.0 40.0 24.0 41.0 56-57 34.58071623931225 38.5 34.0 40.0 23.0 41.0 58-59 34.32884449926436 38.0 34.0 40.0 22.5 41.0 60-61 34.03895387743396 38.0 34.0 40.0 22.0 41.0 62-63 33.729835804504305 38.0 33.5 40.0 20.5 41.0 64-65 33.31673910357 37.0 33.0 40.0 19.0 41.0 66-67 32.970695114472846 36.5 32.5 39.5 18.0 41.0 68-69 32.50240277560803 36.0 32.0 39.0 13.5 41.0 70-71 32.036522538991186 35.5 31.5 39.0 9.5 40.0 72-73 31.574991431149577 35.0 31.0 38.0 7.5 40.0 74-75 31.090958638683638 35.0 31.0 37.5 4.5 39.5 76-77 30.60483259847183 35.0 30.0 37.0 2.0 39.0 78-79 30.0930087507199 34.5 30.0 36.0 2.0 39.0 80-81 29.59282862998655 34.0 29.0 36.0 2.0 38.0 82-83 29.102426325373823 34.0 29.0 35.5 2.0 37.0 84-85 28.56728004868506 34.0 28.5 35.0 2.0 37.0 86-87 28.104406487610724 34.0 27.5 35.0 2.0 36.0 88-89 27.64474303941205 33.5 26.5 35.0 2.0 36.0 90-91 27.14650344269596 33.0 26.0 35.0 2.0 35.5 92-93 26.635533565411222 33.0 25.0 35.0 2.0 35.0 94-95 26.09429466119815 32.5 24.5 35.0 2.0 35.0 96-97 25.379022987835732 32.0 21.0 34.5 2.0 35.0 98-99 24.48316658078386 32.0 10.0 34.0 2.0 35.0 100-101 21.98257317331928 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.06106109175392049 1101 2 -0.03732865600138524 1101 3 -0.03632121914077757 1101 4 -0.0031144248516028483 1101 5 -0.125148862312372 1101 6 -0.1281964670882374 1101 7 -0.1458626526958824 1101 8 -0.24803300353639202 1101 9 -0.09209045679495631 1101 10-11 -0.24894892616554642 1101 12-13 -0.15166380081965514 1101 14-15 -0.253107710956165 1101 16-17 -0.2721362653726942 1101 18-19 -0.19263535607794324 1101 20-21 -0.14578618640084784 1101 22-23 -0.1609389700148398 1101 24-25 -0.2784745320474329 1101 26-27 -0.17758619040808554 1101 28-29 -0.26376419203143797 1101 30-31 -0.19563195504711217 1101 32-33 -0.5078432767269518 1101 34-35 -0.31305381538351185 1101 36-37 -0.09603628460912716 1101 38-39 0.018334271697369786 1101 40-41 -0.23214954926822173 1101 42-43 -0.033675054640937674 1101 44-45 0.09367530947569946 1101 46-47 0.1586348490205367 1101 48-49 0.04618597654835099 1101 50-51 0.16486399114815953 1101 52-53 0.15200914356589124 1101 54-55 -0.07731962889989319 1101 56-57 -0.04795628092539772 1101 58-59 0.09825623119618143 1101 60-61 0.06692020558522671 1101 62-63 -0.013616211830346003 1101 64-65 -0.06963677263662049 1101 66-67 -0.03532525374925655 1101 68-69 -0.07752350769445826 1101 70-71 -0.026996454167324657 1101 72-73 -0.11700668444738582 1101 74-75 -0.12107724628882721 1101 76-77 -0.07753273858970644 1101 78-79 -0.0949849985176563 1101 80-81 -0.2697940121746676 1101 82-83 -0.3044525468206807 1101 84-85 -0.34984050680147405 1101 86-87 -0.038034462123270885 1101 88-89 -0.35824434006584127 1101 90-91 -0.498918729489116 1101 92-93 -0.24418772302721692 1101 94-95 -0.2500216692112538 1101 96-97 -0.12673743830702477 1101 98-99 0.0037548992858909003 1101 100-101 0.47472507499031735 1102 1 -0.12511694015193342 1102 2 -0.04041824220909618 1102 3 0.034194851817503036 1102 4 -0.012493245069919112 1102 5 -0.004459771905942489 1102 6 0.04852969835268084 1102 7 -0.016372565445863074 1102 8 -0.010537769596538737 1102 9 -0.1299457493819105 1102 10-11 -0.007623810764648908 1102 12-13 0.0019016052486691137 1102 14-15 0.11924868159162116 1102 16-17 0.11055674532293835 1102 18-19 0.0508303918364561 1102 20-21 0.05956451045938849 1102 22-23 -0.022297392150846918 1102 24-25 -0.07550031397610724 1102 26-27 -0.06607512332161747 1102 28-29 0.07178740654772042 1102 30-31 0.004107893408551888 1102 32-33 -0.10269606279211274 1102 34-35 0.2517551032744194 1102 36-37 0.26032350674553584 1102 38-39 0.18229001645405418 1102 40-41 0.1312797341914873 1102 42-43 0.12873622457615852 1102 44-45 0.14936058048272827 1102 46-47 0.31124376741642124 1102 48-49 0.19669684578600055 1102 50-51 0.2023900816423705 1102 52-53 0.029414611792809353 1102 54-55 0.3491241761390924 1102 56-57 0.11187673991161518 1102 58-59 0.1600518861216358 1102 60-61 0.38945833079077374 1102 62-63 0.28155588388655417 1102 64-65 0.35062163626578524 1102 66-67 0.24510350167684436 1102 68-69 0.23192482166223982 1102 70-71 0.15208784646330287 1102 72-73 0.2474862002015108 1102 74-75 0.38633455047981613 1102 76-77 0.3629727454913301 1102 78-79 0.16446326532619082 1102 80-81 0.3490712003686838 1102 82-83 0.41854567516045194 1102 84-85 0.43758469389715415 1102 86-87 0.31001502366633105 1102 88-89 0.32253445054066177 1102 90-91 0.40038604810129286 1102 92-93 0.10043806099786678 1102 94-95 0.25951110621461027 1102 96-97 0.4555526028219461 1102 98-99 0.22103909011697453 1102 100-101 0.14655186162411837 1103 1 -0.029782960486535615 1103 2 -0.12508774279327994 1103 3 -0.11222836310448159 1103 4 -0.1091406046377088 1103 5 -0.23330551674840905 1103 6 -0.22010275690815462 1103 7 -0.21414879644604667 1103 8 -0.1684085197560492 1103 9 -0.0953214091792276 1103 10-11 -0.2013321548011362 1103 12-13 -0.0675418034072095 1103 14-15 -0.07962824739195895 1103 16-17 -0.1115080370371686 1103 18-19 -0.04919432898400089 1103 20-21 -0.14917519725489825 1103 22-23 -0.08191461993871485 1103 24-25 -0.12409537036443652 1103 26-27 -0.36991334143382915 1103 28-29 -0.2622184988281546 1103 30-31 -0.20673791772438221 1103 32-33 -0.3554913108703701 1103 34-35 -0.1296878468723861 1103 36-37 -0.24128818026402143 1103 38-39 -0.08968665482532856 1103 40-41 0.06268567216017829 1103 42-43 -0.06178120014963895 1103 44-45 -0.16035884511036613 1103 46-47 0.09609114742377045 1103 48-49 0.111011581880895 1103 50-51 0.03224316539209582 1103 52-53 0.09454983053755228 1103 54-55 0.2568588927335256 1103 56-57 0.16659744988253777 1103 58-59 0.1348532218745646 1103 60-61 0.18581332177250687 1103 62-63 0.2443310238561125 1103 64-65 0.1503104853803947 1103 66-67 0.3583000004352854 1103 68-69 0.46944218838387286 1103 70-71 0.28827401771920336 1103 72-73 0.21261396126856624 1103 74-75 0.16478213819729604 1103 76-77 0.20909530551872635 1103 78-79 0.16838821346771127 1103 80-81 0.11012237384807833 1103 82-83 0.08994520505054737 1103 84-85 -0.009912564715772731 1103 86-87 0.09809958820700615 1103 88-89 -0.06874158573127076 1103 90-91 0.1505731805689834 1103 92-93 0.13560584493513517 1103 94-95 -0.2809889172632616 1103 96-97 0.008150636757143559 1103 98-99 0.15808800419624092 1103 100-101 0.7735271104205346 1104 1 0.03836233864589289 1104 2 0.029487981149813436 1104 3 -0.1310255084442744 1104 4 -0.2699230610369767 1104 5 -0.07562002633145681 1104 6 -0.011255207750501484 1104 7 0.016533379396562964 1104 8 -0.05570621551697741 1104 9 0.1310786257585832 1104 10-11 -0.05646033289963981 1104 12-13 -0.0026154514117635586 1104 14-15 -0.03138808908752111 1104 16-17 -0.008066888426476737 1104 18-19 -0.034912745277061674 1104 20-21 0.026794289385158265 1104 22-23 -0.12520287044281986 1104 24-25 -0.04267106720060809 1104 26-27 0.018320672634665414 1104 28-29 0.0013835293720845243 1104 30-31 -0.0029811993487456334 1104 32-33 0.10164172539115413 1104 34-35 0.3119991374372262 1104 36-37 0.05771548971220142 1104 38-39 0.2777066530777006 1104 40-41 0.37161047604912056 1104 42-43 0.3916080688218244 1104 44-45 0.2902715414494139 1104 46-47 0.31061533809386077 1104 48-49 0.36430544954188093 1104 50-51 0.3285591086301949 1104 52-53 0.24325765856520576 1104 54-55 0.3690646131978994 1104 56-57 0.3651500154775178 1104 58-59 0.4375539749952537 1104 60-61 0.25998351108425055 1104 62-63 0.38205226529296255 1104 64-65 0.6473562072302315 1104 66-67 0.6399751932332762 1104 68-69 0.49128622719496917 1104 70-71 0.5429337731244033 1104 72-73 0.4942910364917843 1104 74-75 0.5836914007835716 1104 76-77 0.5487789376054941 1104 78-79 0.4085182815211468 1104 80-81 0.5194101846176622 1104 82-83 0.6201276346440814 1104 84-85 0.7809834998107519 1104 86-87 0.671901951629529 1104 88-89 0.6401379646552279 1104 90-91 0.8609929312756464 1104 92-93 1.0038861516047923 1104 94-95 0.9505203962237836 1104 96-97 0.8763097152422255 1104 98-99 0.7702824301092548 1104 100-101 1.165999593128575 1105 1 -0.020481590266555827 1105 2 -0.13541765939058692 1105 3 -0.20597170476122173 1105 4 -0.0998124094376891 1105 5 -0.20939602882961594 1105 6 -0.16561509103397754 1105 7 -0.09416372646350624 1105 8 -0.3828098551801986 1105 9 -0.06288924188945799 1105 10-11 -0.16428110916341154 1105 12-13 -0.07615722056630858 1105 14-15 -0.11375460377791313 1105 16-17 -0.01868325933961046 1105 18-19 -0.11989118065105941 1105 20-21 -0.2125361833104975 1105 22-23 -0.27431027459851265 1105 24-25 -0.08783987699174389 1105 26-27 -0.11517758281573265 1105 28-29 -0.3131599023417664 1105 30-31 -0.16696337018515095 1105 32-33 -0.062233348066662586 1105 34-35 -0.09343426956969125 1105 36-37 0.04678528032701479 1105 38-39 0.039600935019194594 1105 40-41 -0.020402020998837145 1105 42-43 0.13422011504081865 1105 44-45 0.2964510209640068 1105 46-47 0.4641321727818095 1105 48-49 0.28501219808899947 1105 50-51 -0.0054332854944334485 1105 52-53 0.026475695489871498 1105 54-55 0.15882853605327085 1105 56-57 0.29002493123150686 1105 58-59 0.1695064975112075 1105 60-61 0.3220478150179815 1105 62-63 0.27218761597999475 1105 64-65 0.43529869005345745 1105 66-67 0.4837730781188938 1105 68-69 0.33446310420402625 1105 70-71 0.34741083584737 1105 72-73 0.41014544996298596 1105 74-75 0.473505851632936 1105 76-77 0.3356892841537835 1105 78-79 0.464283455022823 1105 80-81 0.590839428557242 1105 82-83 0.6979866442797054 1105 84-85 0.5490368617661545 1105 86-87 0.5820919729783895 1105 88-89 0.380980354189969 1105 90-91 0.5614360711981767 1105 92-93 0.6435709479353946 1105 94-95 0.6436424691896612 1105 96-97 0.6466890125735816 1105 98-99 0.6102809079926637 1105 100-101 0.8468309669329486 1106 1 -0.18100942612898763 1106 2 -0.15946382747147325 1106 3 -0.20124358482534177 1106 4 0.13409521577496974 1106 5 0.022801537408838612 1106 6 -0.11430893253925234 1106 7 -0.4783721921332855 1106 8 -0.3649648455150327 1106 9 -0.5871460948542335 1106 10-11 -0.9233967418062221 1106 12-13 -0.40386702320724055 1106 14-15 -0.5953293328283706 1106 16-17 -0.3537665939897252 1106 18-19 -0.5857229206799488 1106 20-21 -0.3971549577916491 1106 22-23 -0.4259147277746749 1106 24-25 -0.35776496095844124 1106 26-27 -0.6857328779413621 1106 28-29 -0.27550085720077533 1106 30-31 -0.27041842671015104 1106 32-33 -0.238929054133159 1106 34-35 -0.19815694681274465 1106 36-37 -0.5837844818273084 1106 38-39 -0.2672234117259862 1106 40-41 -0.12417047128861469 1106 42-43 -0.3678011430639714 1106 44-45 -0.19780825487693932 1106 46-47 -0.3038480263505292 1106 48-49 -0.10910943029446685 1106 50-51 -0.4017570098413543 1106 52-53 -0.26507048575882663 1106 54-55 -0.5361541790077169 1106 56-57 -0.3623480828440009 1106 58-59 -0.36995390338702094 1106 60-61 -0.5324062389981137 1106 62-63 -0.49773966262092273 1106 64-65 -0.6339982018177963 1106 66-67 -0.46000566061605497 1106 68-69 -0.5210513970609654 1106 70-71 -0.44948323003730195 1106 72-73 -0.5710443402475427 1106 74-75 -0.4116432342580225 1106 76-77 -0.10709660206584815 1106 78-79 -0.41684769456169235 1106 80-81 -0.521309451915954 1106 82-83 -0.4556812039780205 1106 84-85 -0.6031443412796484 1106 86-87 -0.47233583321258266 1106 88-89 -0.18435522293737705 1106 90-91 0.013880981490306254 1106 92-93 -0.04782397912374137 1106 94-95 0.20791408765338204 1106 96-97 0.5024681867239487 1106 98-99 0.46858566718861994 1106 100-101 0.7554586884988304 1107 1 0.05670539789040063 1107 2 0.014581072013918117 1107 3 0.04970103143011784 1107 4 0.12390017756681715 1107 5 0.27208106738348903 1107 6 0.1781771073856362 1107 7 0.2714454953800143 1107 8 0.2571514667526884 1107 9 0.11396988480940706 1107 10-11 0.12500799811184038 1107 12-13 0.10899711112349308 1107 14-15 0.02152419122551663 1107 16-17 -0.01760354361776706 1107 18-19 0.07996194418155511 1107 20-21 0.17345936814795948 1107 22-23 0.03641527745195816 1107 24-25 0.09464476595501026 1107 26-27 0.21725363816514687 1107 28-29 0.2611558313433875 1107 30-31 0.31913987093538054 1107 32-33 0.2636434628534161 1107 34-35 0.3612190796633712 1107 36-37 0.40117447978687437 1107 38-39 0.42309171514904165 1107 40-41 0.5773700434007125 1107 42-43 0.6135208652036184 1107 44-45 0.49941925251368957 1107 46-47 0.35391941522168224 1107 48-49 0.2617250134406959 1107 50-51 0.4164325083785343 1107 52-53 0.3828551803273328 1107 54-55 0.4356374827525187 1107 56-57 0.3891135158454162 1107 58-59 0.37185029816691895 1107 60-61 0.6776802380635658 1107 62-63 0.5803463764776282 1107 64-65 0.5128155130392784 1107 66-67 0.29778006385141964 1107 68-69 0.3445357524394055 1107 70-71 0.5330204523650508 1107 72-73 0.6111219865845179 1107 74-75 0.7378473237111507 1107 76-77 0.5603406318250634 1107 78-79 0.589981534933063 1107 80-81 0.768764175472441 1107 82-83 0.5641526385907589 1107 84-85 0.4277196649675261 1107 86-87 0.609046808159647 1107 88-89 0.41745106513907615 1107 90-91 0.5319510927783959 1107 92-93 0.7230118074229388 1107 94-95 0.7992836314486631 1107 96-97 0.7089192598369358 1107 98-99 0.9980510835944756 1107 100-101 1.3299248176277167 1108 1 0.1494232091282619 1108 2 0.15923999318933468 1108 3 0.09436100529990199 1108 4 0.10966073170513368 1108 5 0.06971461043693239 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0.1527731845531548 2316 2 0.13231515853239273 2316 3 0.030431229752569067 2316 4 -0.11590859166019385 2316 5 0.15217056925422412 2316 6 0.20735080459542843 2316 7 0.1720519561434699 2316 8 0.27791296627583506 2316 9 0.12699167337957817 2316 10-11 -0.14869410249217196 2316 12-13 0.16553490487792288 2316 14-15 0.12759980318843844 2316 16-17 0.07196557579142393 2316 18-19 0.4353283883162504 2316 20-21 0.2035745467848855 2316 22-23 0.4853796478821053 2316 24-25 0.5351052359620994 2316 26-27 0.319331010820612 2316 28-29 0.27702651825801894 2316 30-31 0.13034839073095128 2316 32-33 -0.11927659681450109 2316 34-35 -0.010397089363110013 2316 36-37 -0.2807392951868337 2316 38-39 -0.3307047781383119 2316 40-41 -0.5018794614045134 2316 42-43 -0.7100552969757246 2316 44-45 -0.7023695621978803 2316 46-47 -0.34318644641722784 2316 48-49 -0.5044696898092482 2316 50-51 -0.5764269052130331 2316 52-53 -0.29155119729231416 2316 54-55 -0.2963276457535784 2316 56-57 -0.5362526837486428 2316 58-59 -0.5022051636480569 2316 60-61 -0.8584445265660747 2316 62-63 -0.6462144040960709 2316 64-65 -0.47290942765962285 2316 66-67 -0.6170280940240147 2316 68-69 -0.43891740580283667 2316 70-71 -0.13461214979179914 2316 72-73 -0.6841975057491361 2316 74-75 -0.932047279277139 2316 76-77 -0.6631572773525498 2316 78-79 -0.7522408503162445 2316 80-81 -0.5567071765927061 2316 82-83 -1.0327893808257649 2316 84-85 -0.7262920240746062 2316 86-87 -0.7747413707462805 2316 88-89 -0.5313115592709714 2316 90-91 -0.20922710397899635 2316 92-93 -0.5289200635574254 2316 94-95 -0.4314037722634616 2316 96-97 -0.47447398595085133 2316 98-99 -0.38170217490942804 2316 100-101 0.3399570969909327 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3994.0 3 1037.0 4 737.0 5 882.0 6 891.0 7 1038.0 8 1390.0 9 1752.0 10 2045.0 11 2718.0 12 3479.0 13 4428.0 14 5123.0 15 6450.0 16 7805.0 17 10009.0 18 8563.0 19 5862.0 20 5742.0 21 6232.0 22 6568.0 23 7242.0 24 7965.0 25 8972.0 26 10393.0 27 12072.0 28 14016.0 29 16802.0 30 20239.0 31 25003.0 32 31889.0 33 40937.0 34 55687.0 35 79362.0 36 116037.0 37 166970.0 38 139141.0 39 18285.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.866794069346795 19.25229821577806 15.498477596414023 22.382430118461116 2 14.7927051273962 19.911805326733923 41.9140861428735 23.381403402996376 3 18.408997176114863 25.158164439610097 30.51490046301435 25.917937921260688 4 12.171403534394626 16.914619541404996 38.568599386592666 32.34537753760771 5 12.892027994905769 37.23672987720097 35.41075163284144 14.46049049505182 6 30.521687501753142 37.05353386130097 18.252751259922768 14.172027377023122 7 27.299406740438425 32.86314357442392 21.930968710116268 17.90648097502139 8 25.43292142788694 35.01679330791938 20.53481485290371 19.015470411289964 9 25.618558929767136 16.920696614271407 21.305211051500518 36.15553340446094 10-11 23.76703939864246 26.997396174199217 28.3809415845472 20.854622842611118 12-13 25.08759463609951 24.497455723511827 28.830789835983573 21.584159804405093 14-15 22.164905687716615 25.79349220705467 27.36990139920119 24.671700706027526 16-17 20.884963095372356 28.992294175841447 28.15708264404375 21.965660084742446 18-19 20.840515151054806 27.088550497867946 31.184260805446456 20.88667354563079 20-21 21.50230627441014 26.251274366322257 31.434797722645307 20.811621636622295 22-23 21.198977579785293 26.18511648287446 31.354297816402543 21.261608120937705 24-25 20.99774148734684 26.418171854936425 31.450518943815265 21.133567713901478 26-27 20.77056868380171 26.638288076961054 31.33208967649927 21.25905356273797 28-29 20.787971467822413 26.64820540269533 31.41250874319462 21.15131438628763 30-31 20.905044588413965 26.410564015242045 31.417286899753933 21.267104496590058 32-33 20.739276827944234 26.491298397586853 31.2924605590857 21.476964215383212 34-35 21.11925161159118 26.562575787591964 31.10413353927105 21.214039061545805 36-37 21.14535463193084 26.433489789456093 31.071788705947785 21.349366872665282 38-39 20.82921690847194 26.654685947212 31.109772563022926 21.406324581293134 40-41 21.167431198020243 26.607969509103103 31.020830460985533 21.203768831891125 42-43 20.93645258291556 26.537769673841222 30.818548874741403 21.707228868501815 44-45 21.07662964655395 26.703449146332485 30.806300626065696 21.41362058104787 46-47 21.274583996844363 26.98838551330188 30.168680331341584 21.568350158512175 48-49 21.195573724026893 27.086775229451625 29.927270920933058 21.79038012558842 50-51 21.41695838422563 27.177245445991833 29.87896886852548 21.526827301257065 52-53 21.406007592204837 27.3267933281191 29.59592027132721 21.671278808348855 54-55 21.265443588423437 27.21537074254871 29.777979331991933 21.74120633703592 56-57 21.27099097499077 27.57476829889559 29.538736031332814 21.615504694780824 58-59 21.380342851401657 27.2029286124431 29.640109419914403 21.77661911624084 60-61 21.326632473120917 27.225866068075177 29.905392405107655 21.54210905369625 62-63 21.127553693798 27.205305502770926 30.07460206100534 21.592538742425738 64-65 21.12440852345375 27.122379352218918 29.957144417530312 21.79606770679702 66-67 21.1142237325835 27.254258989057412 29.654280951340695 21.9772363270184 68-69 21.13445299612604 27.133948306720313 29.83117438420883 21.900424312944818 70-71 21.150901878409535 27.196778834611525 29.747939018427772 21.904380268551172 72-73 21.084086097883535 27.131943698040555 29.666419769240477 22.117550434835437 74-75 21.160873108367433 27.483635790082456 29.369295163320803 21.986195938229304 76-77 21.206415160259237 27.282542460913056 29.457074033978074 22.053968344849626 78-79 21.19101379594388 27.265844304170916 29.456136609930862 22.087005289954345 80-81 21.099439829092084 27.454742057916846 29.29426877805163 22.151549334939443 82-83 21.256860127870738 27.48514064905061 29.194718103143614 22.063281119935045 84-85 21.222965268431285 27.305254131149994 29.238809911021384 22.232970689397337 86-87 21.07611346155419 27.282977378494554 29.408774961530238 22.232134198421015 88-89 21.166123873643624 27.089217647831994 29.33740306398142 22.40725541454296 90-91 21.188107242803646 27.122801072661733 29.45636615390008 22.232725530634543 92-93 21.12999650669511 26.914804252251663 29.54991278421073 22.405286456842497 94-95 20.940924111881362 26.96855855861119 29.511306195815166 22.579211133692283 96-97 20.907979302339033 27.078310279133326 29.403145778063916 22.61056464046373 98-99 20.903749820343748 27.026551733405313 29.37984414601058 22.68985430024036 100-101 21.08109623639678 26.716133090888277 29.609644375623173 22.59312629709177 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1307.0 1 793.0 2 285.5 3 283.5 4 224.0 5 191.0 6 327.0 7 713.0 8 1208.0 9 1430.0 10 1283.0 11 1089.5 12 1098.5 13 1174.5 14 1251.0 15 1339.0 16 1378.0 17 1424.5 18 1549.5 19 1704.5 20 1876.0 21 2146.0 22 2543.5 23 3054.0 24 3679.0 25 4554.5 26 5937.0 27 7623.5 28 8898.5 29 10158.0 30 12004.0 31 13985.5 32 15938.0 33 17918.0 34 20107.5 35 22086.5 36 24507.0 37 26917.5 38 28122.0 39 28848.0 40 29159.0 41 29897.0 42 30682.0 43 31207.5 44 32298.0 45 33099.0 46 33051.0 47 32695.5 48 32169.5 49 31007.0 50 29399.5 51 27341.5 52 25716.5 53 24814.0 54 23858.5 55 21865.0 56 19091.5 57 16884.0 58 15222.0 59 13223.0 60 10792.5 61 8662.5 62 6839.0 63 5054.0 64 3550.5 65 2629.0 66 1941.5 67 1470.5 68 1062.5 69 736.5 70 536.0 71 382.0 72 284.0 73 231.0 74 179.5 75 122.0 76 84.5 77 61.5 78 43.5 79 30.5 80 22.0 81 10.0 82 9.5 83 10.0 84 6.0 85 5.5 86 4.0 87 2.5 88 3.0 89 1.0 90 1.0 91 1.0 92 1.0 93 1.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.25683234665255233 2 0.22430568994984598 3 0.2150956330340259 4 0.21952578699353428 5 0.22010870198820648 6 0.2506534477090275 7 0.2501871157132898 8 0.24016097780492868 9 0.23538107484861698 10-11 0.24412479976869936 12-13 0.24622329374951907 14-15 0.23130066988591189 16-17 0.2455820872553797 18-19 0.23421524485927264 20-21 0.2262293094322641 22-23 0.22681222442693627 24-25 0.21975895299140316 26-27 0.22716197342373956 28-29 0.24394992527029769 30-31 0.24464942326390426 32-33 0.24610671075058466 34-35 0.2517609861989046 36-37 0.2246554389466493 38-39 0.22599614343439522 40-41 0.22104136597968194 42-43 0.22529664544078867 44-45 0.24447454876550262 46-47 0.25012882421382254 48-49 0.2547921441711998 50-51 0.255666516663208 52-53 0.2806152784351763 54-55 0.25467556117226536 56-57 0.2231398599605017 58-59 0.20862527659316496 60-61 0.2038453736368533 62-63 0.21177301756439465 64-65 0.20326245864218112 66-67 0.20343733314058277 68-69 0.20821723609689446 70-71 0.20920819158783713 72-73 0.21008256407984535 74-75 0.21573683952816528 76-77 0.22634589243119854 78-79 0.22040015948554253 80-81 0.21666950351964076 82-83 0.21165643456546018 84-85 0.21241422405853397 86-87 0.22045845098500977 88-89 0.21515392453349313 90-91 0.2265207669296002 92-93 0.2139298030446816 94-95 0.21375492854627995 96-97 0.22296498546210006 98-99 0.22815292891468225 100-101 0.20477803762832875 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 857758.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.59696716618475 #Duplication Level Percentage of deduplicated Percentage of total 1 84.88185571261133 57.37756013610219 2 9.1896594454426 12.42386215604006 3 2.390520440784342 4.8477579523737795 4 1.075766418043052 2.9087418895576143 5 0.5872354755336485 1.9847668579233466 6 0.36870711369574244 1.4954089595057907 7 0.26921769350299557 1.273880971179459 8 0.1776514251177126 0.9606958040566358 9 0.1442247515453208 0.8774240215284184 >10 0.8454363141841883 10.136235380217766 >50 0.051605246001081906 2.3898772314685313 >100 0.017084537090635572 1.837048543319665 >500 3.4514214912684336E-4 0.17381269079034284 >1k 6.902842982536867E-4 1.3129274059364489 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4346 0.5066697133690388 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3377 0.39370078740157477 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2271 0.2647599905800937 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1261 0.147011161656318 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 916 0.10679002702393915 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 2.3316599786886278E-4 0.0 4 0.0 0.0 0.0 2.3316599786886278E-4 0.0 5 0.0 0.0 0.0 3.497489968032942E-4 0.0 6 0.0 0.0 0.0 3.497489968032942E-4 0.0 7 0.0 0.0 0.0 3.497489968032942E-4 0.0 8 0.0 0.0 0.0 4.6633199573772557E-4 0.0 9 0.0 0.0 0.0 4.6633199573772557E-4 0.0 10-11 0.0 0.0 0.0 5.82914994672157E-4 0.0 12-13 0.0 0.0 0.0 6.994979936065884E-4 0.0 14-15 0.0 0.0 0.0 6.994979936065884E-4 0.0 16-17 0.0 0.0 0.0 6.994979936065884E-4 0.0 18-19 0.0 0.0 0.0 6.994979936065884E-4 0.0 20-21 0.0 0.0 0.0 7.57789493073804E-4 0.0 22-23 0.0 0.0 0.0 0.0012824129882787452 0.0 24-25 0.0 0.0 0.0 0.0013989959872131768 0.0 26-27 0.0 0.0 0.0 0.0017487449840164707 0.0 28-29 0.0 0.0 0.0 0.0025065344770902748 0.0 30-31 0.0 0.0 0.0 0.004022113463237883 0.0 32-33 0.0 0.0 0.0 0.005887441446188785 0.0 34-35 0.0 0.0 0.0 0.00839397592327906 0.0 36-37 0.0 0.0 0.0 0.011191967897705414 0.0 38-39 0.0 0.0 0.0 0.014689457865738356 0.0 40-41 0.0 0.0 0.0 0.01923619482418118 0.0 42-43 0.0 0.0 0.0 0.027863336745329103 0.0 44-45 0.0 0.0 0.0 0.04575882708176432 0.0 46-47 1.1658299893443139E-4 0.0 0.0 0.06272165342672409 0.0 48-49 1.1658299893443139E-4 0.0 0.0 0.10160208357135696 0.0 50-51 1.1658299893443139E-4 0.0 0.0 0.13937497522611272 0.0 52-53 1.1658299893443139E-4 0.0 0.0 0.17539912189685203 0.0 54-55 1.1658299893443139E-4 0.0 0.0 0.23281624887205948 0.0 56-57 1.1658299893443139E-4 0.0 0.0 0.2948966958046442 0.0 58-59 1.1658299893443139E-4 0.0 0.0 0.3702093131162869 0.0 60-61 1.1658299893443139E-4 0.0 0.0 0.45438223834694635 0.0 62-63 1.1658299893443139E-4 0.0 0.0 0.56589387682773 0.0 64-65 1.1658299893443139E-4 0.0 0.0 0.6656306324161361 0.0 66-67 1.1658299893443139E-4 0.0 0.0 0.8008669111800764 0.0 68-69 1.1658299893443139E-4 0.0 0.0 0.9657152716733625 0.0 70-71 1.748744984016471E-4 0.0 0.0 1.103691250912262 0.0 72-73 2.914574973360785E-4 0.0 0.0 1.2848029397569012 0.0 74-75 3.497489968032942E-4 0.0 0.0 1.5008895282818697 0.0 76-77 3.497489968032942E-4 0.0 0.0 1.6948253470092962 0.0 78-79 3.497489968032942E-4 0.0 0.0 1.8898687042266 0.0 80-81 4.6633199573772557E-4 0.0 0.0 2.153287990318948 0.0 82-83 4.6633199573772557E-4 0.0 0.0 2.475756565371585 0.0 84-85 4.6633199573772557E-4 0.0 0.0 2.8301688821322566 0.0 86-87 4.6633199573772557E-4 0.0 0.0 3.260068690702972 0.0 88-89 4.6633199573772557E-4 0.0 0.0 3.804044963731029 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2055 0.0 52.02026 1 GTATCAA 3770 0.0 44.61324 1 CCATGCG 70 4.303254E-6 40.722054 9 ATCAACG 4760 0.0 34.827217 3 TCAACGC 4780 0.0 34.68555 4 TATCAAC 4870 0.0 34.333176 2 CAACGCA 4905 0.0 33.60988 5 AACGCAG 5025 0.0 32.712708 6 CGCAGAG 5725 0.0 28.629913 8 ACGCAGA 5735 0.0 28.579992 7 GCAGAGT 6010 0.0 26.560806 9 GTAGTAC 180 6.692062E-9 26.389296 3 GGACCGA 150 1.0578206E-6 25.338165 6 GTACATG 3250 0.0 25.144728 1 TACATGG 3275 0.0 24.366789 2 GAGTACT 3950 0.0 24.175497 12-13 ACATGGG 3260 0.0 23.167534 3 ACCGAGT 250 3.8198777E-10 22.80435 8 GTACTTT 4260 0.0 22.137438 14-15 AGTACTT 4140 0.0 22.090565 12-13 >>END_MODULE