FastQCFastQC Report
Thu 26 May 2016
SRR937464_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937464_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences857758
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38620.450243541884774No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32390.3776123335486233No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20700.24132680779427296No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8760.10212670706656189No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21600.051.278011
GTATCAA38100.039.6762961
ATCAACG48200.031.2400673
TCAACGC48500.031.046834
TATCAAC49850.030.301332
CAACGCA49700.030.2972095
AACGCAG51600.029.2719636
CGCAGAG58400.025.8650918
ACGCAGA58350.025.805857
GCAGAGT62000.023.826959
GAGTACT39600.022.610812-13
GTACATG33000.021.895691
TACATGG32900.021.6568412
AACCGCG550.002878675521.58995476-77
AGTACTT40300.021.56978412-13
GTACTTT41750.021.21762714-15
TAAGGTG4750.021.0002985
ACATGGG32400.020.6715893
AGAGTAC58850.020.66299210-11
CAGAGTA61800.020.48370410-11
Produced by FastQC (version 0.11.5)