##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937464_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 857758 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.26698439419976 33.0 31.0 34.0 31.0 34.0 2 32.4734353978628 34.0 31.0 34.0 31.0 34.0 3 32.521181965076394 34.0 31.0 34.0 31.0 34.0 4 36.14919709288634 37.0 35.0 37.0 35.0 37.0 5 36.07460962182807 37.0 35.0 37.0 35.0 37.0 6 36.11763108009485 37.0 35.0 37.0 35.0 37.0 7 36.06398891062514 37.0 35.0 37.0 35.0 37.0 8 36.03488279911117 37.0 35.0 37.0 35.0 37.0 9 37.75801682992173 39.0 37.0 39.0 35.0 39.0 10-11 37.674457131265456 39.0 37.0 39.0 35.0 39.0 12-13 37.587902998281564 39.0 37.0 39.0 35.0 39.0 14-15 38.814332830472 40.0 38.0 41.0 35.0 41.0 16-17 38.779563116869795 40.0 38.0 41.0 35.0 41.0 18-19 38.77313298156356 40.0 38.0 41.0 35.0 41.0 20-21 38.67941190872017 40.0 38.0 41.0 34.0 41.0 22-23 38.532613511036914 40.0 38.0 41.0 34.0 41.0 24-25 38.47432317739968 40.0 38.0 41.0 34.0 41.0 26-27 38.42761886219657 40.0 38.0 41.0 34.0 41.0 28-29 38.304752039619565 40.0 38.0 41.0 34.0 41.0 30-31 38.379616978215296 40.0 38.0 41.0 34.0 41.0 32-33 38.31978366858718 40.0 38.0 41.0 34.0 41.0 34-35 38.199816265193675 40.0 38.0 41.0 34.0 41.0 36-37 38.03730597674402 40.0 38.0 41.0 33.5 41.0 38-39 37.90242760778681 40.0 37.0 41.0 33.0 41.0 40-41 37.73327325422788 40.0 37.0 41.0 33.0 41.0 42-43 37.5239822887108 40.0 37.0 41.0 33.0 41.0 44-45 37.23280225891219 40.0 37.0 41.0 32.0 41.0 46-47 36.89663984480471 40.0 36.5 41.0 31.0 41.0 48-49 36.574157862707196 40.0 36.0 41.0 30.5 41.0 50-51 36.347807889871035 39.0 36.0 41.0 30.0 41.0 52-53 36.11942121204349 39.0 35.5 41.0 29.5 41.0 54-55 35.902261477013326 39.0 35.0 40.5 29.0 41.0 56-57 35.653467528137305 39.0 35.0 40.0 28.0 41.0 58-59 35.396269110868104 38.5 35.0 40.0 28.0 41.0 60-61 35.08323384917424 38.0 34.5 40.0 27.5 41.0 62-63 34.725318213295594 38.0 34.0 40.0 26.5 41.0 64-65 34.342393775400524 37.0 34.0 40.0 26.0 41.0 66-67 33.940724540021776 37.0 33.5 39.5 26.0 41.0 68-69 33.514207970080136 36.0 33.0 39.0 25.0 40.0 70-71 33.102626265217 36.0 32.5 39.0 24.0 40.0 72-73 32.625317980129594 35.0 32.0 38.0 22.5 40.0 74-75 32.147762539084454 35.0 31.5 37.0 22.0 39.0 76-77 30.88888649246058 34.0 30.5 36.0 20.5 38.5 78-79 31.477145068888895 34.5 31.0 36.0 21.0 39.0 80-81 31.271271151070582 35.0 31.0 36.0 20.0 38.0 82-83 30.92097479708729 34.0 31.0 35.5 19.0 37.0 84-85 30.54857314067604 34.0 31.0 35.0 18.0 37.0 86-87 30.138573467108436 34.0 30.5 35.0 13.0 36.0 88-89 29.682701880950106 34.0 30.0 35.0 7.0 36.0 90-91 29.1855144457994 34.0 29.0 35.0 2.0 35.5 92-93 28.83129449098697 34.0 29.0 35.0 2.0 35.0 94-95 28.411707031587 34.0 29.0 35.0 2.0 35.0 96-97 27.947336544806344 34.0 29.0 35.0 2.0 35.0 98-99 27.217673283140467 33.0 27.0 35.0 2.0 35.0 100-101 24.757282357028437 31.0 21.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.012942729571534528 1101 2 -0.02596912227700443 1101 3 0.046241517429358225 1101 4 0.01769847939169722 1101 5 0.09948491145394911 1101 6 0.03069098733634945 1101 7 0.05648792554192994 1101 8 -0.032124701072412165 1101 9 -0.02354954448306046 1101 10-11 -0.003608027238442446 1101 12-13 -0.0402487426324214 1101 14-15 -0.07997046150591558 1101 16-17 -0.06540631055482748 1101 18-19 -0.051275188617005085 1101 20-21 -0.03936887264503497 1101 22-23 0.027535044954291266 1101 24-25 -0.039461127980047195 1101 26-27 -0.06710634775669178 1101 28-29 -0.16938780147309984 1101 30-31 -0.18265855263287278 1101 32-33 -0.14541684385962128 1101 34-35 -0.06892959376097707 1101 36-37 0.0876776579991656 1101 38-39 -0.12431423043008749 1101 40-41 -0.22268766652209138 1101 42-43 -0.27766478545864004 1101 44-45 -0.17782094624241296 1101 46-47 -0.3282953107984028 1101 48-49 -0.2679861825039822 1101 50-51 -0.18165615286928016 1101 52-53 -0.3054109309888915 1101 54-55 -0.5187386547677377 1101 56-57 -0.46757597231036385 1101 58-59 -0.36475490134511546 1101 60-61 -0.5037223223080147 1101 62-63 -0.8300182063204602 1101 64-65 -0.669707910007638 1101 66-67 -0.5444775086051266 1101 68-69 -0.586548224110679 1101 70-71 -0.44091500048308063 1101 72-73 -0.46440414627631554 1101 74-75 -0.550223957700176 1101 76-77 -0.24386903133945026 1101 78-79 -0.42041801617792984 1101 80-81 -0.3126843227727676 1101 82-83 -0.15044545202765747 1101 84-85 -0.2639789390710021 1101 86-87 -0.31268956909295653 1101 88-89 -0.17659096553432718 1101 90-91 -0.15884872300999575 1101 92-93 -0.19896805590408917 1101 94-95 -0.0657922986935553 1101 96-97 -0.2685525950290959 1101 98-99 -0.05886613643430394 1101 100-101 0.9118967748568174 1102 1 -0.09359623943957729 1102 2 0.005020423181711919 1102 3 -0.13748862726232858 1102 4 -0.02181297815138805 1102 5 -0.008592299375592916 1102 6 -0.05703596556907797 1102 7 -0.0688257172479183 1102 8 -0.12152223176190802 1102 9 -0.09886443201714457 1102 10-11 -0.09411665593648166 1102 12-13 -0.16600361449582834 1102 14-15 -0.11194761324286162 1102 16-17 -0.1846829438230415 1102 18-19 -0.1285987002632254 1102 20-21 -0.03586000416995461 1102 22-23 -0.05518443293446751 1102 24-25 -0.11841525456922142 1102 26-27 0.029004612674853547 1102 28-29 -0.08963638344736324 1102 30-31 -0.09695561936032959 1102 32-33 -0.05707701820242761 1102 34-35 -0.058979501453364946 1102 36-37 -0.092243569344852 1102 38-39 -0.1601251943993276 1102 40-41 -0.05805713936636181 1102 42-43 0.08444015565186191 1102 44-45 0.07974742624795539 1102 46-47 0.0771018346755099 1102 48-49 0.15718929367620404 1102 50-51 0.3011382062119168 1102 52-53 0.16847204208925604 1102 54-55 0.25493408756103264 1102 56-57 0.04991235657465154 1102 58-59 -0.020440958506391382 1102 60-61 0.13416585773178724 1102 62-63 0.1903382753248053 1102 64-65 0.004274249531881935 1102 66-67 0.09055365585965092 1102 68-69 0.1700141985022654 1102 70-71 0.12413697086421394 1102 72-73 0.16048693860344798 1102 74-75 0.30121090483420687 1102 76-77 0.264391812020623 1102 78-79 0.1454024209500382 1102 80-81 -0.006423848905193097 1102 82-83 0.04199790228289402 1102 84-85 0.20631261933567302 1102 86-87 0.12402861688231326 1102 88-89 0.23780535316736362 1102 90-91 0.18245130096770978 1102 92-93 0.030803867645026628 1102 94-95 0.3273670456013882 1102 96-97 0.525593569389823 1102 98-99 0.51481518807838 1102 100-101 1.1789638560888314 1103 1 -0.02213787675890444 1103 2 0.009800242153488625 1103 3 -0.07909501103442551 1103 4 -0.01313583206653135 1103 5 -0.024171940467944353 1103 6 -0.11383446740449443 1103 7 -0.012169463708978867 1103 8 -0.15291905479121226 1103 9 -0.2197437198365506 1103 10-11 -0.14581424434448564 1103 12-13 -0.03181651517337514 1103 14-15 -0.1999675445769853 1103 16-17 -0.14411593935120237 1103 18-19 -0.15358053943702288 1103 20-21 -0.16168414660607056 1103 22-23 -0.13198677409283732 1103 24-25 -0.19351544984989033 1103 26-27 -0.16885448141065496 1103 28-29 -0.24325832890688304 1103 30-31 -0.3329034211407844 1103 32-33 -0.2149008126135854 1103 34-35 -0.07374915588668074 1103 36-37 -0.12329236741580019 1103 38-39 -0.1433758027524803 1103 40-41 -0.14330253839354867 1103 42-43 -0.18139636100499956 1103 44-45 -0.2319966207298947 1103 46-47 -0.23931292821176697 1103 48-49 -0.24956556693428666 1103 50-51 -0.1357877942907848 1103 52-53 -0.08756867683086966 1103 54-55 -0.12061867827065242 1103 56-57 0.015740310712693883 1103 58-59 -0.09530568451959454 1103 60-61 0.014307857439000315 1103 62-63 0.02093091211104081 1103 64-65 -0.02380528798161663 1103 66-67 0.17384624817603367 1103 68-69 0.12001046966341988 1103 70-71 0.08454636974776975 1103 72-73 0.09825399942219093 1103 74-75 0.19193383001159958 1103 76-77 0.419452122536228 1103 78-79 0.03124540092755268 1103 80-81 0.08884245924052436 1103 82-83 0.03127887167050858 1103 84-85 0.024519986968801533 1103 86-87 0.17425124237155032 1103 88-89 0.25691910484879443 1103 90-91 0.2346819752246354 1103 92-93 0.4174236890474603 1103 94-95 0.4535937645270387 1103 96-97 0.6000474326049705 1103 98-99 0.5456153968933535 1103 100-101 1.2300171840084388 1104 1 0.07439514775629874 1104 2 0.08917834909632205 1104 3 0.0592323713252938 1104 4 0.05137111558849483 1104 5 0.012085075330872996 1104 6 0.031171759658150222 1104 7 0.021677294830077187 1104 8 0.013722261882406883 1104 9 0.044978727858385525 1104 10-11 -0.0600641280106089 1104 12-13 -0.038264574543561025 1104 14-15 -0.04067107465136388 1104 16-17 -0.11567023527145892 1104 18-19 -0.012629809281925475 1104 20-21 -0.007473236671835082 1104 22-23 -0.10848958944800557 1104 24-25 -0.09963589193369415 1104 26-27 -0.37745166801320806 1104 28-29 -0.13474969158696837 1104 30-31 -0.18778274262437833 1104 32-33 -0.0907552454238143 1104 34-35 0.014765866619598 1104 36-37 0.0399026051535003 1104 38-39 -0.014099497365883451 1104 40-41 -0.09304630267174474 1104 42-43 -0.12176489468610896 1104 44-45 0.09708331005709425 1104 46-47 -0.1721389604961132 1104 48-49 -0.0180445824108304 1104 50-51 -0.005270217709963276 1104 52-53 0.03718792558736794 1104 54-55 0.19442814893927363 1104 56-57 0.01159972818491184 1104 58-59 -0.11456327106974129 1104 60-61 -0.06025914711043612 1104 62-63 -0.10870041867323721 1104 64-65 -0.015032809941985192 1104 66-67 -0.2963707728634546 1104 68-69 -0.27224063983801017 1104 70-71 -0.15270735255987233 1104 72-73 -0.20802712647892463 1104 74-75 -0.11694165433641501 1104 76-77 0.05687405836209081 1104 78-79 -0.14456257362808245 1104 80-81 -0.13059448478518476 1104 82-83 -0.023466195245944732 1104 84-85 0.04505122242706605 1104 86-87 0.05940749119503508 1104 88-89 0.2076718490482925 1104 90-91 0.16042481786744034 1104 92-93 0.204597445931487 1104 94-95 0.17954088795540102 1104 96-97 0.07421378176924875 1104 98-99 0.3459695187269247 1104 100-101 0.8590664689127472 1105 1 0.08184724227503182 1105 2 0.03625733674756759 1105 3 -0.021799152964625534 1105 4 -0.044372371674768374 1105 5 -0.1079455866878476 1105 6 -0.07247418033122699 1105 7 -0.05443873439141811 1105 8 -0.05065150090749171 1105 9 -0.12209082894677437 1105 10-11 -0.01620245545554866 1105 12-13 -0.0743705138581845 1105 14-15 -0.050739797430779277 1105 16-17 -0.07873733158299245 1105 18-19 -0.05780999571766898 1105 20-21 -0.0035114182603379618 1105 22-23 -0.11681533181329229 1105 24-25 -0.14555085269211787 1105 26-27 -0.10427410840546969 1105 28-29 -0.07893195014123222 1105 30-31 -0.27750523880143163 1105 32-33 -0.19453260527335914 1105 34-35 -0.002241040180571474 1105 36-37 -0.07800337040946914 1105 38-39 -0.23633087321019985 1105 40-41 -0.2892871454535779 1105 42-43 -0.18797461253747372 1105 44-45 -0.17531122256288256 1105 46-47 -0.16639650105384618 1105 48-49 -0.04188963721879446 1105 50-51 -0.08196998648946874 1105 52-53 -0.007335161547530333 1105 54-55 0.16548008057583985 1105 56-57 -0.09019613401174809 1105 58-59 -0.07807523341540445 1105 60-61 -0.07296568224740696 1105 62-63 -0.03209440913725814 1105 64-65 0.06099652140160572 1105 66-67 0.047571870017392826 1105 68-69 0.1694799482011362 1105 70-71 0.11150815718050211 1105 72-73 0.23951281142813485 1105 74-75 0.21415832597022444 1105 76-77 0.39574075625568383 1105 78-79 0.32967560309593225 1105 80-81 0.31718397036063095 1105 82-83 0.2003065197942142 1105 84-85 0.2118763775221133 1105 86-87 0.2685341493409865 1105 88-89 0.18349530235422762 1105 90-91 0.3459351309919292 1105 92-93 0.42900058400236674 1105 94-95 0.5103450755021335 1105 96-97 0.41559197203539 1105 98-99 0.8770077513141885 1105 100-101 1.0925728841634132 1106 1 -0.0824316623742547 1106 2 0.094313312209799 1106 3 0.02732729483555829 1106 4 0.008513923449108063 1106 5 -0.06139388373948407 1106 6 -0.15127208261206704 1106 7 -0.07847173466706892 1106 8 -0.0944871245610841 1106 9 -0.032528684806706565 1106 10-11 -0.1571993636548754 1106 12-13 -0.08085829150135737 1106 14-15 -0.06946748897406252 1106 16-17 0.12662732005832567 1106 18-19 -0.0937374744692292 1106 20-21 0.017375793342772283 1106 22-23 -0.15803758505301602 1106 24-25 -0.22573313023082875 1106 26-27 -0.2633078372996849 1106 28-29 -0.11954014472978969 1106 30-31 -0.19727862965173415 1106 32-33 0.024674482166140876 1106 34-35 -0.046074908126804814 1106 36-37 -0.08339558665642954 1106 38-39 0.051081933972746185 1106 40-41 0.022974627003243597 1106 42-43 -0.151810552218393 1106 44-45 0.2341192961959777 1106 46-47 0.23387888164646853 1106 48-49 -0.03376641816445414 1106 50-51 -0.0760989010585007 1106 52-53 0.043824395382159764 1106 54-55 -0.0509077797611468 1106 56-57 -0.14881569153838115 1106 58-59 -0.005234007299605992 1106 60-61 0.15067149380264055 1106 62-63 0.16192222794573752 1106 64-65 0.11889623890829171 1106 66-67 0.03410515559548344 1106 68-69 0.19384609394943197 1106 70-71 0.29660580084564714 1106 72-73 0.2612489075425728 1106 74-75 0.36346631391202777 1106 76-77 0.17974793007598322 1106 78-79 0.1725972932802975 1106 80-81 0.2982245254450717 1106 82-83 0.23272741200958436 1106 84-85 0.3246906710869233 1106 86-87 0.5685450347266539 1106 88-89 0.6817641748916259 1106 90-91 0.47665325041865714 1106 92-93 0.6090650535768383 1106 94-95 0.7219797970732813 1106 96-97 0.7600077691879825 1106 98-99 0.8647340400472885 1106 100-101 1.2539601387813626 1107 1 -0.07956321038803083 1107 2 -0.056620768139907796 1107 3 -0.11273207809212238 1107 4 -0.06922556698491178 1107 5 -0.015417050336949956 1107 6 -0.15348668868281123 1107 7 -2.3023931215959692E-4 1107 8 -0.013535215806157908 1107 9 -0.07083393272070282 1107 10-11 -0.08975562229276335 1107 12-13 -0.039509509637987605 1107 14-15 -0.0907329752061159 1107 16-17 -0.09308091798703089 1107 18-19 0.05536986575170033 1107 20-21 0.026672697232591247 1107 22-23 0.1095176096649837 1107 24-25 0.0963040594505955 1107 26-27 -0.07004317328145504 1107 28-29 0.17484494055940303 1107 30-31 0.09270616559015821 1107 32-33 0.018085409346504377 1107 34-35 2.665502491367988E-4 1107 36-37 0.09657369394779636 1107 38-39 -1.8041214622144253E-4 1107 40-41 0.05156050186560179 1107 42-43 0.09407786221042613 1107 44-45 0.224413351259507 1107 46-47 -0.08223082812032345 1107 48-49 0.005702427575421609 1107 50-51 0.1539577655875064 1107 52-53 -0.10521012313727596 1107 54-55 0.0291356653560797 1107 56-57 -0.11295332765298127 1107 58-59 -0.2854328053800046 1107 60-61 -0.11352408979357165 1107 62-63 -0.05056375000322788 1107 64-65 0.13924912977490322 1107 66-67 0.22402363660710023 1107 68-69 0.3231139104759322 1107 70-71 0.3126735735758288 1107 72-73 0.2774550931521844 1107 74-75 0.3409008828385396 1107 76-77 0.42144109144680897 1107 78-79 0.2936305764131397 1107 80-81 0.45331977209888663 1107 82-83 0.29175534042326845 1107 84-85 0.1843436266150711 1107 86-87 0.3663682339647636 1107 88-89 0.35814143468846993 1107 90-91 0.45591249361008224 1107 92-93 0.6181693156605945 1107 94-95 0.6884756167977351 1107 96-97 0.669924225653002 1107 98-99 0.9408998586768362 1107 100-101 1.4700660266999819 1108 1 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0.0031034658704989226 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 4.0 4 5.0 5 11.0 6 27.0 7 77.0 8 194.0 9 339.0 10 679.0 11 1083.0 12 1761.0 13 2414.0 14 3300.0 15 4480.0 16 5995.0 17 8391.0 18 7712.0 19 4637.0 20 3944.0 21 4011.0 22 4237.0 23 4666.0 24 5255.0 25 6144.0 26 7478.0 27 9230.0 28 11488.0 29 14315.0 30 18095.0 31 22691.0 32 29382.0 33 38958.0 34 53916.0 35 78205.0 36 121796.0 37 193623.0 38 171567.0 39 17642.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.95970373343008 20.481660660685183 15.619440481287352 21.939195124597383 2 15.49912679333798 21.231629433942906 40.21215774146088 23.057086031258233 3 18.92550113202092 25.570265739287773 30.33699481671987 25.167238311971442 4 12.99399131223492 17.857017946786858 37.49763919427158 31.651351546706646 5 13.116384375757804 38.10099516872167 34.56252681452741 14.220093640993117 6 30.807873549416037 37.773591152749376 17.501789549033646 13.916745748800944 7 27.741274345444754 33.4440483213214 21.049177040610523 17.765500292623326 8 25.902760452248767 35.98380895310799 19.6737308191821 18.439699775461143 9 26.071805800703697 17.34137134249987 20.593570680774764 35.99325217602167 10-11 24.21269722280676 27.74045487374301 27.5634228674988 20.48342503595143 12-13 25.274729758956006 25.15242684997724 28.151762869932778 21.421080521133977 14-15 22.446599157338085 26.55614986977679 26.511032249189164 24.48621872369596 16-17 21.257219402209017 29.849444715176077 27.277623758682516 21.61571212393239 18-19 21.22778219497807 28.056981106559192 30.10691826832277 20.608318430139967 20-21 21.811454979143303 27.14489401439567 30.622156832113482 20.421494174347544 22-23 21.70595957311372 27.04471953235573 30.302327262383038 20.94699363214751 24-25 21.51475195015803 27.355899322763833 30.385449856660035 20.743898870418104 26-27 21.32177680210363 27.49615420263024 30.30208527031919 20.87998372494694 28-29 21.117549331079307 27.669882538109537 30.326853012329124 20.885715118482032 30-31 21.342516219666454 27.55868517233942 30.224714516382882 20.87408409161124 32-33 21.31586065067303 27.51487016151409 30.15430925738961 21.014959930423267 34-35 21.57974626876112 27.613499378612616 30.034112185488215 20.77264216713805 36-37 21.704794187168286 27.369273949805162 30.0088311673171 20.91710069570945 38-39 21.349069779913588 27.676991305199394 30.02818990060087 20.945749014286147 40-41 21.70369929945194 27.66628543981571 29.904157004839366 20.725858255892987 42-43 21.497226440778753 27.749583893243475 29.663179434561197 21.09001023141657 44-45 21.530702477917686 27.92214340550028 29.56386806745271 20.983286049129326 46-47 21.870620851102526 28.138647497312764 28.859421888224883 21.13130976335983 48-49 21.77887003094113 28.266539047143834 28.654935308093894 21.299655613821148 50-51 21.820198704063383 28.40935322083851 28.65639259558057 21.114055479517532 52-53 21.94103698245892 28.482858801666673 28.43779947257851 21.138304743295894 54-55 21.850102243290063 28.517600535349132 28.4360507275945 21.196246493766306 56-57 21.798630849260515 28.856973645247265 28.29405263489236 21.050342870599867 58-59 21.767153439548217 28.390058734514863 28.3261712510988 21.516616574838125 60-61 21.61909303090149 28.564058860424502 28.715500176040326 21.10134793263368 62-63 21.567913094369274 28.510605555413065 28.865017872173738 21.056463478043923 64-65 21.603949834306317 28.497623162723727 28.63122735738248 21.267199645587475 66-67 21.535386437666567 28.56114428545114 28.46012511687446 21.443344160007836 68-69 21.60737643950858 28.485715085140562 28.52581963677401 21.381088838576847 70-71 21.674003623399607 28.53421361269729 28.376010483143265 21.415772280759843 72-73 21.6839714698085 28.505942235455688 28.2276586169992 21.58242767773661 74-75 21.698311178677436 28.918412885685708 27.88263123165275 21.500644703984108 76-77 21.728447883901985 28.595769436134667 28.187903814362556 21.487878865600788 78-79 21.797173561773832 28.63027800381926 28.064267543992592 21.508280890414312 80-81 21.652519303252554 28.941296894108703 27.900092916758474 21.506090885880266 82-83 21.73667204698775 28.93178767142851 27.85385812157427 21.47768216000947 84-85 21.69364014941312 28.952872378625155 27.746993317446922 21.606494154514806 86-87 21.602829702550135 28.896320407387634 27.817111586251603 21.68373830381063 88-89 21.639087015218745 28.68303181083709 27.76937084818795 21.908510325756218 90-91 21.70831181217303 28.846380306010495 27.701376849022306 21.743931032794166 92-93 21.486169104578277 28.772681088750552 27.916181620091507 21.824968186579664 94-95 21.456109998391153 28.796700234798163 27.747569827387213 21.99961993942347 96-97 21.53701859965165 28.923309371640954 27.545881239230646 21.99379078947675 98-99 21.280444228377036 29.058870985605477 27.458476001944604 22.202208784072877 100-101 21.253551819545997 28.989843282703266 27.521369215504066 22.235235682246675 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 298.0 1 209.0 2 148.0 3 179.0 4 177.5 5 181.0 6 267.0 7 550.5 8 869.5 9 1058.5 10 1085.0 11 1058.0 12 1128.0 13 1217.0 14 1331.5 15 1376.0 16 1387.5 17 1476.0 18 1587.0 19 1675.0 20 1820.0 21 2073.0 22 2443.5 23 2919.0 24 3513.5 25 4380.5 26 5690.0 27 7439.0 28 8828.5 29 10291.0 30 12187.5 31 13860.0 32 15945.5 33 18245.0 34 20466.0 35 22263.5 36 24680.5 37 27254.5 38 28712.5 39 29405.5 40 29550.5 41 30322.5 42 30774.0 43 31004.0 44 32206.5 45 33340.5 46 32907.5 47 32152.0 48 32102.5 49 31033.5 50 29561.5 51 27660.0 52 25744.5 53 25087.5 54 24317.0 55 22067.0 56 19068.0 57 16727.5 58 15136.0 59 13306.5 60 10707.0 61 8549.5 62 6924.0 63 5032.5 64 3423.0 65 2501.5 66 1819.0 67 1392.5 68 1046.0 69 726.5 70 517.0 71 372.0 72 303.0 73 244.5 74 184.0 75 132.0 76 85.5 77 59.0 78 48.5 79 31.5 80 18.0 81 12.0 82 10.0 83 7.5 84 6.5 85 4.0 86 0.5 87 1.0 88 1.5 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0649367304064783 2 0.0 3 0.0 4 0.0 5 0.0016321619850820396 6 0.0 7 0.0 8 0.0 9 0.0 10-11 4.080404962705099E-4 12-13 0.006004024445123216 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0013989959872131765 24-25 8.160809925410198E-4 26-27 4.080404962705099E-4 28-29 0.0038472389648362354 30-31 3.4974899680329414E-4 32-33 0.0 34-35 0.0 36-37 5.82914994672157E-5 38-39 4.663319957377256E-4 40-41 1.165829989344314E-4 42-43 0.012299506387582511 44-45 1.7487449840164707E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 5.82914994672157E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.165829989344314E-4 82-83 2.914574973360785E-4 84-85 2.331659978688628E-4 86-87 0.0 88-89 0.0 90-91 0.008743724920082355 92-93 0.002623117476024706 94-95 0.0 96-97 0.0 98-99 1.165829989344314E-4 100-101 0.011541716894508707 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 857758.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.26503962274612 #Duplication Level Percentage of deduplicated Percentage of total 1 83.39722742204397 54.429233521268685 2 9.930173079461797 12.96186278983602 3 2.7728220777409 5.429050283117551 4 1.1734930762294216 3.063522884685257 5 0.647802193475139 2.1139417912428393 6 0.4198980109249361 1.6442796192316942 7 0.28234001609642667 1.2898852636334046 8 0.20718253953452312 1.081742132148945 9 0.17063410998305462 1.002279775413247 >10 0.9194129510449234 10.82378566124918 >50 0.05411160413472978 2.3912635595760983 >100 0.024007130893278772 2.5321784346691483 >500 3.583153747674385E-4 0.16463368860671743 >1k 5.374730621511578E-4 1.0723405953211687 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3862 0.450243541884774 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3239 0.3776123335486233 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2070 0.24132680779427296 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 876 0.10212670706656189 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 1.1658299893443139E-4 0.0 4 0.0 0.0 0.0 1.1658299893443139E-4 0.0 5 0.0 0.0 0.0 1.1658299893443139E-4 0.0 6 0.0 0.0 0.0 1.1658299893443139E-4 0.0 7 0.0 0.0 0.0 1.1658299893443139E-4 0.0 8 0.0 0.0 0.0 2.3316599786886278E-4 0.0 9 0.0 0.0 0.0 2.3316599786886278E-4 0.0 10-11 0.0 0.0 0.0 2.3316599786886278E-4 0.0 12-13 0.0 0.0 0.0 2.3316599786886278E-4 0.0 14-15 0.0 0.0 0.0 2.3316599786886278E-4 0.0 16-17 0.0 0.0 0.0 2.3316599786886278E-4 0.0 18-19 0.0 0.0 0.0 2.914574973360785E-4 0.0 20-21 0.0 0.0 0.0 4.0804049627050985E-4 0.0 22-23 0.0 0.0 0.0 8.160809925410197E-4 0.0 24-25 0.0 0.0 0.0 9.326639914754511E-4 0.0 26-27 0.0 0.0 0.0 0.0012824129882787454 0.0 28-29 0.0 0.0 0.0 0.001981910981885334 0.0 30-31 0.0 0.0 0.0 0.0034391984685657262 0.0 32-33 5.8291499467215696E-5 0.0 0.0 0.005246234952049412 0.0 34-35 1.1658299893443139E-4 0.0 0.0 0.007694477929672472 0.0 36-37 1.1658299893443139E-4 0.0 0.0 0.010317595405697178 0.0 38-39 1.1658299893443139E-4 0.0 0.0 0.013698502374795689 0.0 40-41 1.1658299893443139E-4 0.0 0.0 0.01824523933323851 0.0 42-43 1.1658299893443139E-4 0.0 0.0 0.02722213025118973 0.0 44-45 1.748744984016471E-4 0.0 0.0 0.04529249508602659 0.0 46-47 2.914574973360785E-4 0.0 0.0 0.06266336192725687 0.0 48-49 3.497489968032942E-4 0.0 0.0 0.10189354106869303 0.0 50-51 3.497489968032942E-4 0.0 0.0 0.14019105621865374 0.0 52-53 3.497489968032942E-4 0.0 0.0 0.17650666038672913 0.0 54-55 3.497489968032942E-4 0.0 0.0 0.23386549586246935 0.0 56-57 4.6633199573772557E-4 0.0 0.0 0.2964122747907918 0.0 58-59 4.6633199573772557E-4 0.0 0.0 0.37306559659018046 0.0 60-61 4.6633199573772557E-4 0.0 0.0 0.45712193882190544 0.0 62-63 4.6633199573772557E-4 0.0 0.0 0.5690999092984268 0.0 64-65 4.6633199573772557E-4 0.0 0.0 0.6690698308847017 0.0 66-67 4.6633199573772557E-4 0.0 0.0 0.8040146521513061 0.0 68-69 4.6633199573772557E-4 0.0 0.0 0.9699705511344692 0.0 70-71 4.6633199573772557E-4 0.0 0.0 1.1073053238792294 0.0 72-73 4.6633199573772557E-4 0.0 0.0 1.2886501787217375 0.0 74-75 4.6633199573772557E-4 0.0 0.0 1.5057277227376487 0.0 76-77 4.6633199573772557E-4 0.0 0.0 1.7026364079379033 0.0 78-79 4.6633199573772557E-4 0.0 0.0 1.8986124291466824 0.0 80-81 4.6633199573772557E-4 0.0 0.0 2.1645965412155874 0.0 82-83 5.82914994672157E-4 0.0 0.0 2.4914369787282657 0.0 84-85 5.82914994672157E-4 0.0 0.0 2.850104574950044 0.0 86-87 5.82914994672157E-4 0.0 0.0 3.2872325294546947 0.0 88-89 5.82914994672157E-4 0.0 0.0 3.839544486906563 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2160 0.0 51.27801 1 GTATCAA 3810 0.0 39.676296 1 ATCAACG 4820 0.0 31.240067 3 TCAACGC 4850 0.0 31.04683 4 TATCAAC 4985 0.0 30.30133 2 CAACGCA 4970 0.0 30.297209 5 AACGCAG 5160 0.0 29.271963 6 CGCAGAG 5840 0.0 25.865091 8 ACGCAGA 5835 0.0 25.80585 7 GCAGAGT 6200 0.0 23.82695 9 GAGTACT 3960 0.0 22.6108 12-13 GTACATG 3300 0.0 21.89569 1 TACATGG 3290 0.0 21.656841 2 AACCGCG 55 0.0028786755 21.589954 76-77 AGTACTT 4030 0.0 21.569784 12-13 GTACTTT 4175 0.0 21.217627 14-15 TAAGGTG 475 0.0 21.000298 5 ACATGGG 3240 0.0 20.671589 3 AGAGTAC 5885 0.0 20.662992 10-11 CAGAGTA 6180 0.0 20.483704 10-11 >>END_MODULE